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The map label for this gene is rlmL [H]

Identifier: 83721658

GI number: 83721658

Start: 1411914

End: 1413206

Strand: Reverse

Name: rlmL [H]

Synonym: BTH_I1265

Alternate gene names: 83721658

Gene position: 1413206-1411914 (Counterclockwise)

Preceding gene: 83720920

Following gene: 83720141

Centisome position: 37.1

GC content: 72.08

Gene sequence:

>1293_bases
ATGTCCTCGCCGACCCTATTCGACTTCTTCGCTCCCTGCCCGCGCGGCCTCGAGGCCGCGCTCGCCGCCGAGCTCGCGGA
AATCGCCGCGCGCCACGCGATCGCGCCGTACGACGCGGGCGCGCAAGTGCCGGGCGGCGTCCATTTCCGCGGCGGCTGGG
CGGCCGGCATGGCGGCCAACCTGCATTCGCGGATCGCGAGCCGCGTGCTGCTGAAGATCGCGCATCGCCCATACCGCAAC
GAGCAGGACGTCTATGCGCTCGCGCTCGAGCAGCCGTGGGAGCGCTGGTTCGCGTCGACTCAGACGCTGCGCGTCGACAT
CACCGCGATCAAGTCGCCGCTGAAGAGCCTCGAATTCGCGACGCTGCGCGTGAAGGACGCCATCTGCGACCGCCTGCGCG
ACAAGACGGGCGCGCGTCCGAGCATCGACACCGCGCAGCCCGACGTGCGCGTGTTCGCGTTCCTGACGGCGGGCGACTGC
ACGCTGTATCTCGACACGTCCGGCGAGCCGCTCTTCAAGCGCGGCTGGCGCCTCGACAAGGGCGCGGCGCCGCTGCGCGA
GAACCTCGCGGCCGGCATCCTGCGCCTGACCGGCTGGACGCCCGGCACGCCGCTCTACGATCCGATGTGCGGCAGCGGCA
CGTTCGTCGCGGAAGCCGCGCAGATCGCGCTCGGCGTGGCGCCGGGCGTCGAGCGCCGCTTCGGCTTCGAGAAGCTCAAG
CAATACGACATCACCGCGTGGCAGGCGCTGAAGGTCGCCGCGCTGGACGCGAAGCGCGTGGCGCGCGGCAAGCGCGACGA
TCTGCTGATCTTCGGCAGCGACATCTCCGGCGACATGCTCGAGAAGGCGCGCGCGAACCTCGAGCGCGCGGGCGTGCCGT
CGCTGTGGCTCAAGCAGATCGACGCGCGCAACATGACGCCCCCCGTCGCCGCGCCCGGCGTGATCGTCACGAACCCGCCG
TACGGCGAGCGGATCGAGGTGCGCGGCCGCGGCCCGCGCGGCGACGTGCGCGAGACGGGCAAGAGCCGCGGCGGCGGCGA
CGCGTTCCGCCGCACGCACGCCGATTCGCCCGACGGCGAGTTCTTCCGCGCGCTCGGCGACGCGCTCAAGCAGCGCTTTG
CCGGCTGGCAAGCGTTCATGCTGAGTTCGGACCGCAGCCTGCCGGGCCAGTTGCGGCTGCGCGAATCGGCGAAGACCCCG
CTGTTCAACGGCGCGCTCGAGTGCCGGCTGTTTCGCTTCGACCTGATCGCGGGCAGCGTCCGGCAGCGCCCGGCGACGGC
CGACGGCGAGTAG

Upstream 100 bases:

>100_bases
GGCCGGCGGCCGCGCGACGGACGCAGCCGCGCGACGGCGTGACGATCCGCGCGCGCACCCCGCGTACAATGGCGTACTTT
TGCACGCGACAGCCCGCCTT

Downstream 100 bases:

>100_bases
CGCGTCGCGCGTTTGCGGCGCGTTTGCGGCGCGTCTGTCGCGCATCCGTCGCACCGCGTTCGCGCGGGCCTCGCGACGGC
GGCGCAAACGTCTGCATCTG

Product: THUMP domain-containing protein

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MSSPTLFDFFAPCPRGLEAALAAELAEIAARHAIAPYDAGAQVPGGVHFRGGWAAGMAANLHSRIASRVLLKIAHRPYRN
EQDVYALALEQPWERWFASTQTLRVDITAIKSPLKSLEFATLRVKDAICDRLRDKTGARPSIDTAQPDVRVFAFLTAGDC
TLYLDTSGEPLFKRGWRLDKGAAPLRENLAAGILRLTGWTPGTPLYDPMCGSGTFVAEAAQIALGVAPGVERRFGFEKLK
QYDITAWQALKVAALDAKRVARGKRDDLLIFGSDISGDMLEKARANLERAGVPSLWLKQIDARNMTPPVAAPGVIVTNPP
YGERIEVRGRGPRGDVRETGKSRGGGDAFRRTHADSPDGEFFRALGDALKQRFAGWQAFMLSSDRSLPGQLRLRESAKTP
LFNGALECRLFRFDLIAGSVRQRPATADGE

Sequences:

>Translated_430_residues
MSSPTLFDFFAPCPRGLEAALAAELAEIAARHAIAPYDAGAQVPGGVHFRGGWAAGMAANLHSRIASRVLLKIAHRPYRN
EQDVYALALEQPWERWFASTQTLRVDITAIKSPLKSLEFATLRVKDAICDRLRDKTGARPSIDTAQPDVRVFAFLTAGDC
TLYLDTSGEPLFKRGWRLDKGAAPLRENLAAGILRLTGWTPGTPLYDPMCGSGTFVAEAAQIALGVAPGVERRFGFEKLK
QYDITAWQALKVAALDAKRVARGKRDDLLIFGSDISGDMLEKARANLERAGVPSLWLKQIDARNMTPPVAAPGVIVTNPP
YGERIEVRGRGPRGDVRETGKSRGGGDAFRRTHADSPDGEFFRALGDALKQRFAGWQAFMLSSDRSLPGQLRLRESAKTP
LFNGALECRLFRFDLIAGSVRQRPATADGE
>Mature_429_residues
SSPTLFDFFAPCPRGLEAALAAELAEIAARHAIAPYDAGAQVPGGVHFRGGWAAGMAANLHSRIASRVLLKIAHRPYRNE
QDVYALALEQPWERWFASTQTLRVDITAIKSPLKSLEFATLRVKDAICDRLRDKTGARPSIDTAQPDVRVFAFLTAGDCT
LYLDTSGEPLFKRGWRLDKGAAPLRENLAAGILRLTGWTPGTPLYDPMCGSGTFVAEAAQIALGVAPGVERRFGFEKLKQ
YDITAWQALKVAALDAKRVARGKRDDLLIFGSDISGDMLEKARANLERAGVPSLWLKQIDARNMTPPVAAPGVIVTNPPY
GERIEVRGRGPRGDVRETGKSRGGGDAFRRTHADSPDGEFFRALGDALKQRFAGWQAFMLSSDRSLPGQLRLRESAKTPL
FNGALECRLFRFDLIAGSVRQRPATADGE

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=387, Percent_Identity=31.5245478036176, Blast_Score=190, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 46942; Mature: 46811

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPTLFDFFAPCPRGLEAALAAELAEIAARHAIAPYDAGAQVPGGVHFRGGWAAGMAAN
CCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCHHHHHHHH
LHSRIASRVLLKIAHRPYRNEQDVYALALEQPWERWFASTQTLRVDITAIKSPLKSLEFA
HHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHH
TLRVKDAICDRLRDKTGARPSIDTAQPDVRVFAFLTAGDCTLYLDTSGEPLFKRGWRLDK
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCEEEEEECCCCHHHHCCCCCCC
GAAPLRENLAAGILRLTGWTPGTPLYDPMCGSGTFVAEAAQIALGVAPGVERRFGFEKLK
CCCHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCHHHHH
QYDITAWQALKVAALDAKRVARGKRDDLLIFGSDISGDMLEKARANLERAGVPSLWLKQI
HHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHH
DARNMTPPVAAPGVIVTNPPYGERIEVRGRGPRGDVRETGKSRGGGDAFRRTHADSPDGE
CCCCCCCCCCCCCEEECCCCCCCEEEEECCCCCCCHHHHCCCCCCCHHHHHCCCCCCCHH
FFRALGDALKQRFAGWQAFMLSSDRSLPGQLRLRESAKTPLFNGALECRLFRFDLIAGSV
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEHHHCCCCCCCCCCEEEEEEEHHHHHHH
RQRPATADGE
HCCCCCCCCC
>Mature Secondary Structure 
SSPTLFDFFAPCPRGLEAALAAELAEIAARHAIAPYDAGAQVPGGVHFRGGWAAGMAAN
CCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCHHHHHHHH
LHSRIASRVLLKIAHRPYRNEQDVYALALEQPWERWFASTQTLRVDITAIKSPLKSLEFA
HHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHH
TLRVKDAICDRLRDKTGARPSIDTAQPDVRVFAFLTAGDCTLYLDTSGEPLFKRGWRLDK
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCEEEEEECCCCHHHHCCCCCCC
GAAPLRENLAAGILRLTGWTPGTPLYDPMCGSGTFVAEAAQIALGVAPGVERRFGFEKLK
CCCHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCHHHHH
QYDITAWQALKVAALDAKRVARGKRDDLLIFGSDISGDMLEKARANLERAGVPSLWLKQI
HHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHH
DARNMTPPVAAPGVIVTNPPYGERIEVRGRGPRGDVRETGKSRGGGDAFRRTHADSPDGE
CCCCCCCCCCCCCEEECCCCCCCEEEEECCCCCCCHHHHCCCCCCCHHHHHCCCCCCCHH
FFRALGDALKQRFAGWQAFMLSSDRSLPGQLRLRESAKTPLFNGALECRLFRFDLIAGSV
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEHHHCCCCCCCCCCEEEEEEEHHHHHHH
RQRPATADGE
HCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA