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The map label for this gene is yoaE [H]

Identifier: 83720470

GI number: 83720470

Start: 1245787

End: 1247862

Strand: Direct

Name: yoaE [H]

Synonym: BTH_I1105

Alternate gene names: 83720470

Gene position: 1245787-1247862 (Clockwise)

Preceding gene: 83719756

Following gene: 83721557

Centisome position: 32.7

GC content: 61.99

Gene sequence:

>2076_bases
ATGACCACCCCCGCACAATTCGCCCGCGCAACTTGTCCGCATGATTGTCCCGATACGTGCGCAATTCGTGTCACGGTTGA
GAACGGCAAAGCCATCAAGGTTGCCGGTGATCCAGACCATCCACCGACTCAAGGCGTCCTTTGCACCAAGGTCAGCAGAT
ATGCGGAGCGCGTCCATCACCCGGAACGGCTTACGGTGCCGCTCAAACGAATCGGCAGGAAAGGGGAAGGCCAGTTCGAG
CCAATCAGTTGGGACGAGGCGAACAGAGTGGTGGCGGAGCGCCTGGCGGAAATTGCTCGCCGAGCGCCGGAAGCCATTGT
TCCCTACAGCTATGCAGGAACGATGGGGCTGATTCAGGGTGAAAGCATTGCTCAGCGCTTCTTCAATAAGCTCGGCGCAA
CGCGCCTCGAAAGGACGATCTGCTCGGCGGCCGGTGCGGCAGGGCTGCGCTACACGTACGGTGCCAGCGTCGGGATGCAT
TTCGAATTCTTCGAGGAAAGCGAACTGATCCTGATCTGGGGCGCGAATCCGATCGCCTCCAGTCTGCATTTCTGGACACG
CGCACAAGAAGCGAAGCGTCGCGGCGCACGGTTGATCGCGATCGATCCGTATCGATCGCTGACAGCGGAAAAATGTCATC
AGCACATCGCGCTGAAGCCGGGCACGGACGGCGCGCTCGCGCTCGGCATCATGCACGTGTTGATCGAAGAGGGCTTTCTT
GATCGAGACTACATTCGCGACCACACGCTCGGCTTCGATGCGTTGAAAGCACGCGCGCTGATCTATCCGCCCAAGCGCGT
GGCCGAAATCTGCGGTGTGACGGCCGACGAAGTCGTCGAGTTGGCGCGCCTCTACGGACGCACGCGCAAGGCTTCGATTC
GTCTCAATTACGGCATGCAGCGCGTGCGCGGCGGCGGCAACGCCGTTCGCGCGATCGCCTGTCTGCCGGCGCTCACGGGG
GCATGGCGCGACCGTGCCGGCGGCTTGCTCCTATCGTCGTCAGAGTGGGCGCCCGTGGACGCCGCAGCTATCGGCCGTCC
CGATTTGCTGCCAGATTGGCCGAACAAGCTGCCACGTTCGATCAATATGAACGCAATTGGCGACGCGTTGCTGCATCCCG
GCGATCCCGACTTCGGGCCCAAGATCGAGGCGATCGTCGTATATAACTCGAACCCTGTCGCGGTCGCACCCGATTCGGAG
AAAGTCGCCGCAGGCTTCGCGCGAGAGGATCTGTTCACGGTCGTTCTCGAGCATTTCAAGACAGATACCGCCGATTACGC
CGATATTCTGCTGCCCGCAACGACGCAGTTCGAGCATCTCGATGTCCATAAATCGTATGGGCACACCTACGTGATGGCGA
ACCTGCCGTCGATTGCGCCGGTCGGTGACGCGCGGCCGAACACTGAAATTTTCCGCGGCATCGCGCGCGGTATGGGGCTT
GACGAGCCGGCGTTGTACGACGATGACGAAACGCTCGCTCGCACGTCGTTGCGCTGGCGCGATGAATTGCTGCAAAGCGA
CTGGGACACCCTCAAACAGGCGGGTTGGCTCAAGCTGAAGCTTGCCGATGCGCCGTTTGCGAACGGCGGCTTTCGGACGC
CGTCCGGCAAGTGTGAGTTCTATAGCGAACGGCTCGCAGAAATGGGGCTCGATCCGTTGCCGGACTACCTGCCGCCGCAC
GAGTCGACGGACGGTTCGCCAGAACTGGCCGCCCGCTATCCGCTCGCGATGATCTCGCCGCCGGCCCGCCATTTCCTGAA
CAGTACGTTCGTCAATGTCGCGAGCCTGCGCGCAACGGAAGGGGAGCCGCACCTCGACATCCATCCGAACGATGCCGCAC
GGCGCGGCATCGCCGACGGCGACCCGGTGCGCATCTTCAACGATCGCGGTTCGCTACAGGCCAGAGCCCGCCTGACCGAT
CGCGCACGCGAAGGGGTTGTCGTTGGCCTGTCGATCTGGTGGAAAAAGCTCGCGCCGGACGGGCGCAACGCAAATCAGCT
AACGAGCCAGGCTCTTACGGATCTGGGCCGCGGCGCGACTTTTTATGACTGTCTGGTCGAGGTCGAACACGCGTGA

Upstream 100 bases:

>100_bases
TGCCGACACGCTTACGTCGCCTCGCCACCAGCTCGGATAAAAAATTTCCGTATCCGCCGAGCGTAAAATTGCCCAATCGA
TAAAAACGGCGTAATAAGAC

Downstream 100 bases:

>100_bases
CGCAATTCGACGACGTCCCTCACGGCATCCTTACGCAAAGTGGAAAGGAGTGTCTAACCCGGTTGTCTGGACCGGGCCGA
CCCGTTACGATGCCATTTTT

Product: molybdopterin oxidoreductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 691; Mature: 690

Protein sequence:

>691_residues
MTTPAQFARATCPHDCPDTCAIRVTVENGKAIKVAGDPDHPPTQGVLCTKVSRYAERVHHPERLTVPLKRIGRKGEGQFE
PISWDEANRVVAERLAEIARRAPEAIVPYSYAGTMGLIQGESIAQRFFNKLGATRLERTICSAAGAAGLRYTYGASVGMH
FEFFEESELILIWGANPIASSLHFWTRAQEAKRRGARLIAIDPYRSLTAEKCHQHIALKPGTDGALALGIMHVLIEEGFL
DRDYIRDHTLGFDALKARALIYPPKRVAEICGVTADEVVELARLYGRTRKASIRLNYGMQRVRGGGNAVRAIACLPALTG
AWRDRAGGLLLSSSEWAPVDAAAIGRPDLLPDWPNKLPRSINMNAIGDALLHPGDPDFGPKIEAIVVYNSNPVAVAPDSE
KVAAGFAREDLFTVVLEHFKTDTADYADILLPATTQFEHLDVHKSYGHTYVMANLPSIAPVGDARPNTEIFRGIARGMGL
DEPALYDDDETLARTSLRWRDELLQSDWDTLKQAGWLKLKLADAPFANGGFRTPSGKCEFYSERLAEMGLDPLPDYLPPH
ESTDGSPELAARYPLAMISPPARHFLNSTFVNVASLRATEGEPHLDIHPNDAARRGIADGDPVRIFNDRGSLQARARLTD
RAREGVVVGLSIWWKKLAPDGRNANQLTSQALTDLGRGATFYDCLVEVEHA

Sequences:

>Translated_691_residues
MTTPAQFARATCPHDCPDTCAIRVTVENGKAIKVAGDPDHPPTQGVLCTKVSRYAERVHHPERLTVPLKRIGRKGEGQFE
PISWDEANRVVAERLAEIARRAPEAIVPYSYAGTMGLIQGESIAQRFFNKLGATRLERTICSAAGAAGLRYTYGASVGMH
FEFFEESELILIWGANPIASSLHFWTRAQEAKRRGARLIAIDPYRSLTAEKCHQHIALKPGTDGALALGIMHVLIEEGFL
DRDYIRDHTLGFDALKARALIYPPKRVAEICGVTADEVVELARLYGRTRKASIRLNYGMQRVRGGGNAVRAIACLPALTG
AWRDRAGGLLLSSSEWAPVDAAAIGRPDLLPDWPNKLPRSINMNAIGDALLHPGDPDFGPKIEAIVVYNSNPVAVAPDSE
KVAAGFAREDLFTVVLEHFKTDTADYADILLPATTQFEHLDVHKSYGHTYVMANLPSIAPVGDARPNTEIFRGIARGMGL
DEPALYDDDETLARTSLRWRDELLQSDWDTLKQAGWLKLKLADAPFANGGFRTPSGKCEFYSERLAEMGLDPLPDYLPPH
ESTDGSPELAARYPLAMISPPARHFLNSTFVNVASLRATEGEPHLDIHPNDAARRGIADGDPVRIFNDRGSLQARARLTD
RAREGVVVGLSIWWKKLAPDGRNANQLTSQALTDLGRGATFYDCLVEVEHA
>Mature_690_residues
TTPAQFARATCPHDCPDTCAIRVTVENGKAIKVAGDPDHPPTQGVLCTKVSRYAERVHHPERLTVPLKRIGRKGEGQFEP
ISWDEANRVVAERLAEIARRAPEAIVPYSYAGTMGLIQGESIAQRFFNKLGATRLERTICSAAGAAGLRYTYGASVGMHF
EFFEESELILIWGANPIASSLHFWTRAQEAKRRGARLIAIDPYRSLTAEKCHQHIALKPGTDGALALGIMHVLIEEGFLD
RDYIRDHTLGFDALKARALIYPPKRVAEICGVTADEVVELARLYGRTRKASIRLNYGMQRVRGGGNAVRAIACLPALTGA
WRDRAGGLLLSSSEWAPVDAAAIGRPDLLPDWPNKLPRSINMNAIGDALLHPGDPDFGPKIEAIVVYNSNPVAVAPDSEK
VAAGFAREDLFTVVLEHFKTDTADYADILLPATTQFEHLDVHKSYGHTYVMANLPSIAPVGDARPNTEIFRGIARGMGLD
EPALYDDDETLARTSLRWRDELLQSDWDTLKQAGWLKLKLADAPFANGGFRTPSGKCEFYSERLAEMGLDPLPDYLPPHE
STDGSPELAARYPLAMISPPARHFLNSTFVNVASLRATEGEPHLDIHPNDAARRGIADGDPVRIFNDRGSLQARARLTDR
AREGVVVGLSIWWKKLAPDGRNANQLTSQALTDLGRGATFYDCLVEVEHA

Specific function: Terminal Reductase During Anaerobic Growth On Various Sulfoxide And N-Oxide Compounds. Allows E.Coli To Grow Anaerobically On Me(2)So As Respiratory Oxidant. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=764, Percent_Identity=28.5340314136126, Blast_Score=231, Evalue=2e-61,
Organism=Escherichia coli, GI1787870, Length=765, Percent_Identity=29.0196078431373, Blast_Score=228, Evalue=8e-61,
Organism=Escherichia coli, GI171474008, Length=760, Percent_Identity=27.8947368421053, Blast_Score=226, Evalue=5e-60,
Organism=Escherichia coli, GI3868721, Length=711, Percent_Identity=26.3009845288326, Blast_Score=193, Evalue=3e-50,
Organism=Escherichia coli, GI87081994, Length=706, Percent_Identity=26.3456090651558, Blast_Score=163, Evalue=3e-41,
Organism=Escherichia coli, GI145693196, Length=682, Percent_Identity=26.6862170087977, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI1787231, Length=735, Percent_Identity=24.0816326530612, Blast_Score=139, Evalue=7e-34,
Organism=Escherichia coli, GI1788534, Length=348, Percent_Identity=22.4137931034483, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI3868720, Length=400, Percent_Identity=23.75, Blast_Score=81, Evalue=3e-16,
Organism=Escherichia coli, GI1787741, Length=307, Percent_Identity=23.4527687296417, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI3868719, Length=267, Percent_Identity=25.8426966292135, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787477, Length=297, Percent_Identity=23.5690235690236, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1787778, Length=289, Percent_Identity=24.5674740484429, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 75835; Mature: 75704

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00490 MOLYBDOPTERIN_PROK_2 ; PS00932 MOLYBDOPTERIN_PROK_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPAQFARATCPHDCPDTCAIRVTVENGKAIKVAGDPDHPPTQGVLCTKVSRYAERVHH
CCCHHHHHHCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCC
PERLTVPLKRIGRKGEGQFEPISWDEANRVVAERLAEIARRAPEAIVPYSYAGTMGLIQG
CCCEEECHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECC
ESIAQRFFNKLGATRLERTICSAAGAAGLRYTYGASVGMHFEFFEESELILIWGANPIAS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEECCCCEEEEECCCHHHH
SLHFWTRAQEAKRRGARLIAIDPYRSLTAEKCHQHIALKPGTDGALALGIMHVLIEEGFL
HHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCEEECCCCCCHHHHHHHHHHHHCCCC
DRDYIRDHTLGFDALKARALIYPPKRVAEICGVTADEVVELARLYGRTRKASIRLNYGMQ
CHHHHHHHCCCHHHHHCCEEECCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCHH
RVRGGGNAVRAIACLPALTGAWRDRAGGLLLSSSEWAPVDAAAIGRPDLLPDWPNKLPRS
HHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHCCCCCCCCCCHHHCCCC
INMNAIGDALLHPGDPDFGPKIEAIVVYNSNPVAVAPDSEKVAAGFAREDLFTVVLEHFK
CCCHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHC
TDTADYADILLPATTQFEHLDVHKSYGHTYVMANLPSIAPVGDARPNTEIFRGIARGMGL
CCCCCHHHEEECCCCCCHHEECHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCC
DEPALYDDDETLARTSLRWRDELLQSDWDTLKQAGWLKLKLADAPFANGGFRTPSGKCEF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCHHH
YSERLAEMGLDPLPDYLPPHESTDGSPELAARYPLAMISPPARHFLNSTFVNVASLRATE
HHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHCCCHHHHHHHHHHHHHHHEEECC
GEPHLDIHPNDAARRGIADGDPVRIFNDRGSLQARARLTDRAREGVVVGLSIWWKKLAPD
CCCCEEECCCHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCEEEEHHHHHHHHCCC
GRNANQLTSQALTDLGRGATFYDCLVEVEHA
CCCHHHHHHHHHHHHCCCCCCEEHHEEECCC
>Mature Secondary Structure 
TTPAQFARATCPHDCPDTCAIRVTVENGKAIKVAGDPDHPPTQGVLCTKVSRYAERVHH
CCHHHHHHCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCC
PERLTVPLKRIGRKGEGQFEPISWDEANRVVAERLAEIARRAPEAIVPYSYAGTMGLIQG
CCCEEECHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECC
ESIAQRFFNKLGATRLERTICSAAGAAGLRYTYGASVGMHFEFFEESELILIWGANPIAS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEECCCCEEEEECCCHHHH
SLHFWTRAQEAKRRGARLIAIDPYRSLTAEKCHQHIALKPGTDGALALGIMHVLIEEGFL
HHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCEEECCCCCCHHHHHHHHHHHHCCCC
DRDYIRDHTLGFDALKARALIYPPKRVAEICGVTADEVVELARLYGRTRKASIRLNYGMQ
CHHHHHHHCCCHHHHHCCEEECCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCHH
RVRGGGNAVRAIACLPALTGAWRDRAGGLLLSSSEWAPVDAAAIGRPDLLPDWPNKLPRS
HHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHCCCCCCCCCCHHHCCCC
INMNAIGDALLHPGDPDFGPKIEAIVVYNSNPVAVAPDSEKVAAGFAREDLFTVVLEHFK
CCCHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHC
TDTADYADILLPATTQFEHLDVHKSYGHTYVMANLPSIAPVGDARPNTEIFRGIARGMGL
CCCCCHHHEEECCCCCCHHEECHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCC
DEPALYDDDETLARTSLRWRDELLQSDWDTLKQAGWLKLKLADAPFANGGFRTPSGKCEF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCHHH
YSERLAEMGLDPLPDYLPPHESTDGSPELAARYPLAMISPPARHFLNSTFVNVASLRATE
HHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHCCCHHHHHHHHHHHHHHHEEECC
GEPHLDIHPNDAARRGIADGDPVRIFNDRGSLQARARLTDRAREGVVVGLSIWWKKLAPD
CCCCEEECCCHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCEEEEHHHHHHHHCCC
GRNANQLTSQALTDLGRGATFYDCLVEVEHA
CCCHHHHHHHHHHHHCCCCCCEEHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]