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The map label for this gene is citH [H]

Identifier: 83720354

GI number: 83720354

Start: 1422251

End: 1423534

Strand: Direct

Name: citH [H]

Synonym: BTH_I1274

Alternate gene names: 83720354

Gene position: 1422251-1423534 (Clockwise)

Preceding gene: 83721163

Following gene: 83719281

Centisome position: 37.34

GC content: 69.39

Gene sequence:

>1284_bases
ATGCACGCAGCCGACGCGGCCTTGCCCGCGGCCGATACCGTCTCCCCGCGCGTGTGGCGGGCCGTCGCCGCCGCGTCGAT
CGGCAACGCGCTCGAATGGTTCGATCTCGTCGTCTACGGCTTCTTCGCGGTCACGATCTCGCGACTGTTCTTTCCCGCCG
GCAACGACGCCGTGTCGCTGCTGCTGACGCTCGGCACGTTCGGCGTGTCGTTCTTCATGCGTCCGCTCGGCGCGATCGTG
CTCGGCGCGTACGCGGACCGCCACGGCCGCAAGGCCGCGCTCACGCTGTCGATCCTGCTGATGATGGCGGGCACGCTCGT
CATCGCGGTGCTGCCGACCTACGCGACGATCGGGATCGCGGCGCCGCTCATGCTCGTCGGCGCGCGGCTGATGCAGGGCT
TTTCCGCGGGCGGCGAGTTCGGCAGCGCGACCGCGTTCCTCGCCGAGCACGTGCCGGGGCGGCGCGGCTTTTTCTCGAGC
TGGCAGGTCGCGAGCCAGGGGCTCACGACGCTTCTCGCGGCGATCTTCGGCACGGCGCTCAACGCGTGGCTCACGCCCGC
GCAGATGGCGTCGTGGGGCTGGCGCGTGCCGTTCTGCTTCGGGCTGCTGCTCGGGCCCGTTGCGTACTACATCCGCTCGA
AGGTCGACGAGACACCCGAATTCCTCGCATCGGAGCCCGCCGCGAACCCGCTGCGCGACACGTTCGCCGCGCACAAGGGG
CGGCTTGCCGCCGCGATCGGCGCGGTCGTGCTCGGCACCGTCGCGACCTATCTCGTGCTGTTCATGCCGACATACGGTGT
CAAGGAACTGAAGCTCGCGCCGTCGTCCGCGTTCGCGGCGATCCTCGCCGTCGGCGTGATCCAGATGGGCTTCGCACCGG
TGGTCGGCCACTGGTCCGATCGTCACGGCCGCGTGCGAACGATGTTCGTGCCGGCGCTCGCGATCCTCGTGCTGATCTAT
CCGGCGTTCGCATGGCTCGCCGCGCATCCGACGTTCGGCGCGCTGATCGCCGTGCAGATCGCGTTCGCATTCCTGATGAC
CGGCTATTTCGCCGCGCTGCCGGGCCTGCTGTCCGAGATCTTTCCGGTTCGGACACGCACGACCGGCATGTCGCTCGCGT
ACAACGTCGCGGTGACGATCTTCGGCGGATTCGGCCCGTTCATCATCGCGTGGCTGATCCGCGCGACGGGCACGAAGGCC
GCGCCGAGCTTCTACCTGATCTTCGCCGCGCTCGTGAGCATCGCGGCGCTCGTCGCGCTGCGGCGGCGCTTCGGGTTCCG
CTGA

Upstream 100 bases:

>100_bases
GCGCTGTCCGATTCCGCGCCGCTCTCGCGCGTGCGCCCGGCGCGGGCGGGCAGGCAGGGCGGCCCGCGCCGGACATCGAC
GACAACGATCAGGAGACACC

Downstream 100 bases:

>100_bases
CGCGCGGCGTCCGGCGGGCAAAAGAACGGCGGACGAAAAAAAGCCGGTTCGCGAGGAACCGGCCTTGTCGTGCGGGCAGG
GCGCTTATACCTGCGCGCCT

Product: major facilitator family transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MHAADAALPAADTVSPRVWRAVAAASIGNALEWFDLVVYGFFAVTISRLFFPAGNDAVSLLLTLGTFGVSFFMRPLGAIV
LGAYADRHGRKAALTLSILLMMAGTLVIAVLPTYATIGIAAPLMLVGARLMQGFSAGGEFGSATAFLAEHVPGRRGFFSS
WQVASQGLTTLLAAIFGTALNAWLTPAQMASWGWRVPFCFGLLLGPVAYYIRSKVDETPEFLASEPAANPLRDTFAAHKG
RLAAAIGAVVLGTVATYLVLFMPTYGVKELKLAPSSAFAAILAVGVIQMGFAPVVGHWSDRHGRVRTMFVPALAILVLIY
PAFAWLAAHPTFGALIAVQIAFAFLMTGYFAALPGLLSEIFPVRTRTTGMSLAYNVAVTIFGGFGPFIIAWLIRATGTKA
APSFYLIFAALVSIAALVALRRRFGFR

Sequences:

>Translated_427_residues
MHAADAALPAADTVSPRVWRAVAAASIGNALEWFDLVVYGFFAVTISRLFFPAGNDAVSLLLTLGTFGVSFFMRPLGAIV
LGAYADRHGRKAALTLSILLMMAGTLVIAVLPTYATIGIAAPLMLVGARLMQGFSAGGEFGSATAFLAEHVPGRRGFFSS
WQVASQGLTTLLAAIFGTALNAWLTPAQMASWGWRVPFCFGLLLGPVAYYIRSKVDETPEFLASEPAANPLRDTFAAHKG
RLAAAIGAVVLGTVATYLVLFMPTYGVKELKLAPSSAFAAILAVGVIQMGFAPVVGHWSDRHGRVRTMFVPALAILVLIY
PAFAWLAAHPTFGALIAVQIAFAFLMTGYFAALPGLLSEIFPVRTRTTGMSLAYNVAVTIFGGFGPFIIAWLIRATGTKA
APSFYLIFAALVSIAALVALRRRFGFR
>Mature_427_residues
MHAADAALPAADTVSPRVWRAVAAASIGNALEWFDLVVYGFFAVTISRLFFPAGNDAVSLLLTLGTFGVSFFMRPLGAIV
LGAYADRHGRKAALTLSILLMMAGTLVIAVLPTYATIGIAAPLMLVGARLMQGFSAGGEFGSATAFLAEHVPGRRGFFSS
WQVASQGLTTLLAAIFGTALNAWLTPAQMASWGWRVPFCFGLLLGPVAYYIRSKVDETPEFLASEPAANPLRDTFAAHKG
RLAAAIGAVVLGTVATYLVLFMPTYGVKELKLAPSSAFAAILAVGVIQMGFAPVVGHWSDRHGRVRTMFVPALAILVLIY
PAFAWLAAHPTFGALIAVQIAFAFLMTGYFAALPGLLSEIFPVRTRTTGMSLAYNVAVTIFGGFGPFIIAWLIRATGTKA
APSFYLIFAALVSIAALVALRRRFGFR

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=430, Percent_Identity=31.6279069767442, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1788942, Length=440, Percent_Identity=33.4090909090909, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI1788292, Length=418, Percent_Identity=31.1004784688995, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1789941, Length=449, Percent_Identity=33.630289532294, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI87082231, Length=484, Percent_Identity=26.0330578512397, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87082404, Length=381, Percent_Identity=22.5721784776903, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45308; Mature: 45308

Theoretical pI: Translated: 10.61; Mature: 10.61

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCC
>Mature Secondary Structure
MHAADAALPAADTVSPRVWRAVAAASIGNALEWFDLVVYGFFAVTISRLFFPAGNDAVSL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LLTLGTFGVSFFMRPLGAIVLGAYADRHGRKAALTLSILLMMAGTLVIAVLPTYATIGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
APLMLVGARLMQGFSAGGEFGSATAFLAEHVPGRRGFFSSWQVASQGLTTLLAAIFGTAL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
NAWLTPAQMASWGWRVPFCFGLLLGPVAYYIRSKVDETPEFLASEPAANPLRDTFAAHKG
HHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHCC
RLAAAIGAVVLGTVATYLVLFMPTYGVKELKLAPSSAFAAILAVGVIQMGFAPVVGHWSD
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEECCHHHHHHHHHHHHHHHCCHHHHCCCCC
RHGRVRTMFVPALAILVLIYPAFAWLAAHPTFGALIAVQIAFAFLMTGYFAALPGLLSEI
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FPVRTRTTGMSLAYNVAVTIFGGFGPFIIAWLIRATGTKAAPSFYLIFAALVSIAALVAL
HCCCCCCCCHHHHHHHHEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
RRRFGFR
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]