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The map label for this gene is murD [H]

Identifier: 83720203

GI number: 83720203

Start: 1260080

End: 1261594

Strand: Direct

Name: murD [H]

Synonym: BTH_I1116

Alternate gene names: 83720203

Gene position: 1260080-1261594 (Clockwise)

Preceding gene: 83719046

Following gene: 83719280

Centisome position: 33.08

GC content: 71.55

Gene sequence:

>1515_bases
ATGTTCGGAGATCGGCAGCGGCCGATGGTGCTCGTGTTGGGGCTCGGCGAATCGGGCCTCGCGATCGCGCGGTGGTGCGC
GAGGCACGGGTGCCGGTTGCGCATCGCCGATACGCGCGAGACGCCGCCCAACCTTGCCGCGCTGACGGCGGCGGGCATCG
ACGCCGAATTCGTCGGCGGCGCGTTTTCGCCGGCGCTCATCGACGGCGGGATCGAGCTCGTCGCGCTGAGCCCCGGGTTG
TCGCCGCTCGCGGAAGACCTCGCGCCGCTCGTCGCGGCCGCGCGCGAGCGGGGCATTCCCGTGTGGGGCGAGCTCGAGTT
CTTCGCGCAGGCGCTCACGACGCTCGGCGCGAACGGCTATGCGCCGAAGGTGATCGCGATCACCGGCACGAACGGCAAGA
CGACGACGACGAGCCTCGCGGGCCTGCTGTGCGAGCGTGCGGGCAAGACGGTCGCGGTCGCGGGCAACATCAGCCCGGCG
ATGCTCGACAAGCTGACCGAGGCGATCGACGCGGCGGCGCTGCCTGACGTGTGGGTGCTCGAGCTGTCGAGCTTCCAGCT
CGACACCGCGCACACGTTCGCGCCCGACGCGGCGACGATCCTCAACATCACGCAGGACCATCTCGACTGGCATGGCGGCT
TCGCCGCGTATGCAGCCGCGAAGGGCCGGATTTTCGGGCCGCGCACGGTGCGCGTGCTCAACCGCGACGATGCCGAAGTG
ATGAAGTTCGCGCCGCCCGCCGCGGCAGCCGACGCGCCGCGCGCGATCACGTTCGGCCTGAACGAGCCCGCCGCCGATGG
CGATTACGGCCTGCTGCGCGAAAACGGCATCGCGTGGCTCGTCGAGGCGGTCGATCGCGACGCGGCCGATGCGCCCGCCA
CGCCGTCGCGCCGCCGCAAGCAGGAAGCGGCGAATCCGCCCGACATCGCGCTCAAGCGCCTGATGCCGGCTGACGCGCTG
CGCATTCGCGGGCTGCACAACGCGGCGAATGCGTTGGCCGCCTATGCGCTCGCGCGCGCGATCGACCTGCCTGCCGCGCC
GTTGCTGCACGGCCTGCGCGAATATCGCGGCGAGCCGCATCGCGTCGAAGTGATCGCGACGCTCGACGGCGTCGATTATG
TCGACGACAGCAAGGGCACGAACGTCGGCGCGACGGTCGCGGCGCTCGACGGCCTTGCGCAGCGCGCGGTGCTGATCGCG
GGCGGCGACGGCAAGGGCCAGGATTTCGAGCCGCTCGCGGCGCCCGTCGCGCGCTGGTGCCGCGCGGTGATGCTGATCGG
CCGCGACGCGCCCGCGCTTCGCGAAGCGCTCGCCGACACGGGCGTGCCGCTCGCCGATCACGCGACGCTCGAAAGCGCGG
TGCGCGCGGCGGGCGCGCTCGCGCAGCCGGGCGATGCGGTGCTGCTGTCGCCCGCATGCGCGAGCCTCGACATGTTCCGC
AATTACGCGCATCGGGCCGACGTTTTCCGCAGCGCGGTCGAAGACATCGCTTTGGAAAAAGGAACGACGCTATGA

Upstream 100 bases:

>100_bases
CAGGTGGTGGTGCGTTTCTGGATCATCACGTTGATGCTGTGCCTGTTCGGTTTGTCCACCCTCAAGCTGCGGTAAAGGGA
AGGTAAGGATGTTTGGCGAG

Downstream 100 bases:

>100_bases
GCTGGTCCGATCGCCTCGTTTCCCGTTTCAATGGCGCCCGTGACGCGGGAGGCGGCGCCGCGCCGCGCACCGCCGCGCGG
GCGGCCTCGGGCGCGCGCGC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRIADTRETPPNLAALTAAGIDAEFVGGAFSPALIDGGIELVALSPGL
SPLAEDLAPLVAAARERGIPVWGELEFFAQALTTLGANGYAPKVIAITGTNGKTTTTSLAGLLCERAGKTVAVAGNISPA
MLDKLTEAIDAAALPDVWVLELSSFQLDTAHTFAPDAATILNITQDHLDWHGGFAAYAAAKGRIFGPRTVRVLNRDDAEV
MKFAPPAAAADAPRAITFGLNEPAADGDYGLLRENGIAWLVEAVDRDAADAPATPSRRRKQEAANPPDIALKRLMPADAL
RIRGLHNAANALAAYALARAIDLPAAPLLHGLREYRGEPHRVEVIATLDGVDYVDDSKGTNVGATVAALDGLAQRAVLIA
GGDGKGQDFEPLAAPVARWCRAVMLIGRDAPALREALADTGVPLADHATLESAVRAAGALAQPGDAVLLSPACASLDMFR
NYAHRADVFRSAVEDIALEKGTTL

Sequences:

>Translated_504_residues
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRIADTRETPPNLAALTAAGIDAEFVGGAFSPALIDGGIELVALSPGL
SPLAEDLAPLVAAARERGIPVWGELEFFAQALTTLGANGYAPKVIAITGTNGKTTTTSLAGLLCERAGKTVAVAGNISPA
MLDKLTEAIDAAALPDVWVLELSSFQLDTAHTFAPDAATILNITQDHLDWHGGFAAYAAAKGRIFGPRTVRVLNRDDAEV
MKFAPPAAAADAPRAITFGLNEPAADGDYGLLRENGIAWLVEAVDRDAADAPATPSRRRKQEAANPPDIALKRLMPADAL
RIRGLHNAANALAAYALARAIDLPAAPLLHGLREYRGEPHRVEVIATLDGVDYVDDSKGTNVGATVAALDGLAQRAVLIA
GGDGKGQDFEPLAAPVARWCRAVMLIGRDAPALREALADTGVPLADHATLESAVRAAGALAQPGDAVLLSPACASLDMFR
NYAHRADVFRSAVEDIALEKGTTL
>Mature_504_residues
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRIADTRETPPNLAALTAAGIDAEFVGGAFSPALIDGGIELVALSPGL
SPLAEDLAPLVAAARERGIPVWGELEFFAQALTTLGANGYAPKVIAITGTNGKTTTTSLAGLLCERAGKTVAVAGNISPA
MLDKLTEAIDAAALPDVWVLELSSFQLDTAHTFAPDAATILNITQDHLDWHGGFAAYAAAKGRIFGPRTVRVLNRDDAEV
MKFAPPAAAADAPRAITFGLNEPAADGDYGLLRENGIAWLVEAVDRDAADAPATPSRRRKQEAANPPDIALKRLMPADAL
RIRGLHNAANALAAYALARAIDLPAAPLLHGLREYRGEPHRVEVIATLDGVDYVDDSKGTNVGATVAALDGLAQRAVLIA
GGDGKGQDFEPLAAPVARWCRAVMLIGRDAPALREALADTGVPLADHATLESAVRAAGALAQPGDAVLLSPACASLDMFR
NYAHRADVFRSAVEDIALEKGTTL

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=493, Percent_Identity=38.1338742393509, Blast_Score=235, Evalue=4e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 52622; Mature: 52622

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRIADTRETPPNLAALTAAGIDAEFVGG
CCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCCHHHCCC
AFSPALIDGGIELVALSPGLSPLAEDLAPLVAAARERGIPVWGELEFFAQALTTLGANGY
CCCHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
APKVIAITGTNGKTTTTSLAGLLCERAGKTVAVAGNISPAMLDKLTEAIDAAALPDVWVL
CCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEE
ELSSFQLDTAHTFAPDAATILNITQDHLDWHGGFAAYAAAKGRIFGPRTVRVLNRDDAEV
EECCEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCEECCCEEEEECCCCHHH
MKFAPPAAAADAPRAITFGLNEPAADGDYGLLRENGIAWLVEAVDRDAADAPATPSRRRK
HHCCCCCCCCCCCCEEEECCCCCCCCCCCCEECCCCCEEHHHHHCCCCCCCCCCCHHHHH
QEAANPPDIALKRLMPADALRIRGLHNAANALAAYALARAIDLPAAPLLHGLREYRGEPH
HHCCCCHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
RVEVIATLDGVDYVDDSKGTNVGATVAALDGLAQRAVLIAGGDGKGQDFEPLAAPVARWC
EEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHHH
RAVMLIGRDAPALREALADTGVPLADHATLESAVRAAGALAQPGDAVLLSPACASLDMFR
HHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHH
NYAHRADVFRSAVEDIALEKGTTL
HHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRIADTRETPPNLAALTAAGIDAEFVGG
CCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCCHHHCCC
AFSPALIDGGIELVALSPGLSPLAEDLAPLVAAARERGIPVWGELEFFAQALTTLGANGY
CCCHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
APKVIAITGTNGKTTTTSLAGLLCERAGKTVAVAGNISPAMLDKLTEAIDAAALPDVWVL
CCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEE
ELSSFQLDTAHTFAPDAATILNITQDHLDWHGGFAAYAAAKGRIFGPRTVRVLNRDDAEV
EECCEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCEECCCEEEEECCCCHHH
MKFAPPAAAADAPRAITFGLNEPAADGDYGLLRENGIAWLVEAVDRDAADAPATPSRRRK
HHCCCCCCCCCCCCEEEECCCCCCCCCCCCEECCCCCEEHHHHHCCCCCCCCCCCHHHHH
QEAANPPDIALKRLMPADALRIRGLHNAANALAAYALARAIDLPAAPLLHGLREYRGEPH
HHCCCCHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
RVEVIATLDGVDYVDDSKGTNVGATVAALDGLAQRAVLIAGGDGKGQDFEPLAAPVARWC
EEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHHH
RAVMLIGRDAPALREALADTGVPLADHATLESAVRAAGALAQPGDAVLLSPACASLDMFR
HHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHH
NYAHRADVFRSAVEDIALEKGTTL
HHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA