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The map label for this gene is cls [H]

Identifier: 83719812

GI number: 83719812

Start: 2277365

End: 2278804

Strand: Reverse

Name: cls [H]

Synonym: BTH_I2012

Alternate gene names: 83719812

Gene position: 2278804-2277365 (Counterclockwise)

Preceding gene: 83721211

Following gene: 83721224

Centisome position: 59.82

GC content: 68.33

Gene sequence:

>1440_bases
ATGACTCTCGACTGGCTCCATCTCGGCACCCTCATCGGCCTCGCGCACGTGCTCGGCGTGATCGCGGCCTGCCATGCGAT
CCTGAACACACGCACGTCGCAAGGCGCGATCGCCTGGGCGGTGTCGTTGACGGCGGTGCCGTACCTGACGCTGATTCCGT
ACCTGTTCCTCGGCCGCAGCAAGTTCTCCGGCTACGTGGACGCCCGCCGCCACGAAACCGCGGCGCTGCGCACCCGCACG
CACCCCGCGCCGTGGGACGCCGAAGGCTCGACGCTCGGCGCGCCGAAAGACGCGCTCGGCATCGCCCGCGTGCGCGCGCT
CACGCGCCTGGTCGGCATGCCGTTCGTCTCGGGCAACGCGGTGCGCACGCTCGTCAACGGCGACGCGACGTTCGCCGCGA
TCCTCGCCGCGATCGACGCCGCGCGCAGCTACGTGATCGTCCAGTTCTTCATCGTCCGCGACGACGCGCTCGGCGCGATG
CTGCGCGACACGCTGATCGCGCGCGCGAAAGCGGGCGTGCGCTGCTATCTGCTGTACGACAGCATCGGCAGCTTCGATCT
GCCGTCGAGCTATGTGCACACGCTGCGCGAAGGCGGCGTCGAAGTGCATCCGTTCGCGACCAACAAGCAGTTCGTCAACC
GCTTCCAGCTCAACTTCCGCAACCACCGGAAGATCGTCGTCGTCGACGGCGAGCGCGCGTTCGTCGGCGGGCACAACGTC
GGCGTCGAATATCTCGGCGGCAATCCGCGCTTGTCGCCGTGGCGCGACACGCACATCGAAGTGCGCGGCCCCGCCGTCGC
GAACATCCAGTACGTGTTCGCCGAGGACTGGCACTGGGCGACCCAGTCGCTGCCGCCGCAGGCGCCGCCGCCCGCGCCCG
TGGCCGACGCCGACATGCACTGCCTCGTCGTGCCGATGGGCCCCGCCGACAAGCAGGAAACGGGCTCGCTCTTCTTCGTG
GAGGCGATCAACGCCGCGCGCGAGCGCGTGTGGATCACGACGCCCTACCTCGTGCCCGACGAAGCGGTGATTTCCGCGCT
GAAGCTCGCGGTGATGCGCGGCGTCGACGTGCGGGTGCTGATTCCGAGCCGGCGCGATCACTATGTCGTGTTCGAGGCAT
CGAAGCTCTATGCGCGCGATCTCGTCGACGCGGGCGTGCGGATCTTCCGCTACCGCCCCGGCTTCCTGCACCAGAAGGTC
ATGCTGATCGACCGGGCTGCCGCCGCCGTCGGCAGCGCGAACCTCGACAACCGGTCGTTCCGGCTGAATTTCGAGATCAT
GGTGCTGACCGTCGACGATGGCTTCGCGACCGAAGTCGAGGCGATGCTCGCGCGCGACTTCGAAGACGCGTTCGAAGTCG
ATCTGAGCGAATACCGGCGCTCGCCCGCGTGGCGGCGCGTCGCGATGCACGTCGCGAGGCTGTTCGCACCGATTCTCTGA

Upstream 100 bases:

>100_bases
GTACGGAGTCGCCGCTTGCGCGCACGCTTCGGTAAGATGCGCGTAAGCGGCGCCCGGTCGCGGCGCGCCGCGTGGCCTCC
CTCTGGACTCCGACGCGCTC

Downstream 100 bases:

>100_bases
CGGCGCGCGGCACGCGATCGGGCGCCGTGCAGCCCGAACGGCCCCGACTGTTCGGGCGGCCCGCACGCCCGATTCGGGCC
GCGCGGCGCGCCGCCGCGCT

Product: cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MTLDWLHLGTLIGLAHVLGVIAACHAILNTRTSQGAIAWAVSLTAVPYLTLIPYLFLGRSKFSGYVDARRHETAALRTRT
HPAPWDAEGSTLGAPKDALGIARVRALTRLVGMPFVSGNAVRTLVNGDATFAAILAAIDAARSYVIVQFFIVRDDALGAM
LRDTLIARAKAGVRCYLLYDSIGSFDLPSSYVHTLREGGVEVHPFATNKQFVNRFQLNFRNHRKIVVVDGERAFVGGHNV
GVEYLGGNPRLSPWRDTHIEVRGPAVANIQYVFAEDWHWATQSLPPQAPPPAPVADADMHCLVVPMGPADKQETGSLFFV
EAINAARERVWITTPYLVPDEAVISALKLAVMRGVDVRVLIPSRRDHYVVFEASKLYARDLVDAGVRIFRYRPGFLHQKV
MLIDRAAAAVGSANLDNRSFRLNFEIMVLTVDDGFATEVEAMLARDFEDAFEVDLSEYRRSPAWRRVAMHVARLFAPIL

Sequences:

>Translated_479_residues
MTLDWLHLGTLIGLAHVLGVIAACHAILNTRTSQGAIAWAVSLTAVPYLTLIPYLFLGRSKFSGYVDARRHETAALRTRT
HPAPWDAEGSTLGAPKDALGIARVRALTRLVGMPFVSGNAVRTLVNGDATFAAILAAIDAARSYVIVQFFIVRDDALGAM
LRDTLIARAKAGVRCYLLYDSIGSFDLPSSYVHTLREGGVEVHPFATNKQFVNRFQLNFRNHRKIVVVDGERAFVGGHNV
GVEYLGGNPRLSPWRDTHIEVRGPAVANIQYVFAEDWHWATQSLPPQAPPPAPVADADMHCLVVPMGPADKQETGSLFFV
EAINAARERVWITTPYLVPDEAVISALKLAVMRGVDVRVLIPSRRDHYVVFEASKLYARDLVDAGVRIFRYRPGFLHQKV
MLIDRAAAAVGSANLDNRSFRLNFEIMVLTVDDGFATEVEAMLARDFEDAFEVDLSEYRRSPAWRRVAMHVARLFAPIL
>Mature_478_residues
TLDWLHLGTLIGLAHVLGVIAACHAILNTRTSQGAIAWAVSLTAVPYLTLIPYLFLGRSKFSGYVDARRHETAALRTRTH
PAPWDAEGSTLGAPKDALGIARVRALTRLVGMPFVSGNAVRTLVNGDATFAAILAAIDAARSYVIVQFFIVRDDALGAML
RDTLIARAKAGVRCYLLYDSIGSFDLPSSYVHTLREGGVEVHPFATNKQFVNRFQLNFRNHRKIVVVDGERAFVGGHNVG
VEYLGGNPRLSPWRDTHIEVRGPAVANIQYVFAEDWHWATQSLPPQAPPPAPVADADMHCLVVPMGPADKQETGSLFFVE
AINAARERVWITTPYLVPDEAVISALKLAVMRGVDVRVLIPSRRDHYVVFEASKLYARDLVDAGVRIFRYRPGFLHQKVM
LIDRAAAAVGSANLDNRSFRLNFEIMVLTVDDGFATEVEAMLARDFEDAFEVDLSEYRRSPAWRRVAMHVARLFAPIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=467, Percent_Identity=28.2655246252677, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1787007, Length=331, Percent_Identity=30.8157099697885, Blast_Score=130, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 52965; Mature: 52833

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLDWLHLGTLIGLAHVLGVIAACHAILNTRTSQGAIAWAVSLTAVPYLTLIPYLFLGRS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCC
KFSGYVDARRHETAALRTRTHPAPWDAEGSTLGAPKDALGIARVRALTRLVGMPFVSGNA
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCE
VRTLVNGDATFAAILAAIDAARSYVIVQFFIVRDDALGAMLRDTLIARAKAGVRCYLLYD
EEEEECCCHHHHHHHHHHHHHCCEEEEEEEEEECCHHHHHHHHHHHHHHCCCEEEEEEEE
SIGSFDLPSSYVHTLREGGVEVHPFATNKQFVNRFQLNFRNHRKIVVVDGERAFVGGHNV
CCCCCCCCHHHHHHHHHCCEEEEEECCCHHHHHHHHCCCCCCCEEEEECCCEEEECCCCC
GVEYLGGNPRLSPWRDTHIEVRGPAVANIQYVFAEDWHWATQSLPPQAPPPAPVADADMH
CEEEECCCCCCCCCCCCEEEEECCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCE
CLVVPMGPADKQETGSLFFVEAINAARERVWITTPYLVPDEAVISALKLAVMRGVDVRVL
EEEEECCCCCCCCCCCEEEEEEHHHHHCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEE
IPSRRDHYVVFEASKLYARDLVDAGVRIFRYRPGFLHQKVMLIDRAAAAVGSANLDNRSF
ECCCCCCEEEEECHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEE
RLNFEIMVLTVDDGFATEVEAMLARDFEDAFEVDLSEYRRSPAWRRVAMHVARLFAPIL
EEEEEEEEEEECCCCHHHHHHHHHHCHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLDWLHLGTLIGLAHVLGVIAACHAILNTRTSQGAIAWAVSLTAVPYLTLIPYLFLGRS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCC
KFSGYVDARRHETAALRTRTHPAPWDAEGSTLGAPKDALGIARVRALTRLVGMPFVSGNA
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCE
VRTLVNGDATFAAILAAIDAARSYVIVQFFIVRDDALGAMLRDTLIARAKAGVRCYLLYD
EEEEECCCHHHHHHHHHHHHHCCEEEEEEEEEECCHHHHHHHHHHHHHHCCCEEEEEEEE
SIGSFDLPSSYVHTLREGGVEVHPFATNKQFVNRFQLNFRNHRKIVVVDGERAFVGGHNV
CCCCCCCCHHHHHHHHHCCEEEEEECCCHHHHHHHHCCCCCCCEEEEECCCEEEECCCCC
GVEYLGGNPRLSPWRDTHIEVRGPAVANIQYVFAEDWHWATQSLPPQAPPPAPVADADMH
CEEEECCCCCCCCCCCCEEEEECCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCE
CLVVPMGPADKQETGSLFFVEAINAARERVWITTPYLVPDEAVISALKLAVMRGVDVRVL
EEEEECCCCCCCCCCCEEEEEEHHHHHCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEE
IPSRRDHYVVFEASKLYARDLVDAGVRIFRYRPGFLHQKVMLIDRAAAAVGSANLDNRSF
ECCCCCCEEEEECHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEE
RLNFEIMVLTVDDGFATEVEAMLARDFEDAFEVDLSEYRRSPAWRRVAMHVARLFAPIL
EEEEEEEEEEECCCCHHHHHHHHHHCHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA