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The map label for this gene is grpE [H]

Identifier: 83719694

GI number: 83719694

Start: 1465523

End: 1466059

Strand: Direct

Name: grpE [H]

Synonym: BTH_I1306

Alternate gene names: 83719694

Gene position: 1465523-1466059 (Clockwise)

Preceding gene: 83719179

Following gene: 83718472

Centisome position: 38.47

GC content: 68.16

Gene sequence:

>537_bases
ATGGAAAACACGCAAGAGAACCCGACTGACCAAACGACTGAAGAAACCGGTCGCGAGGCGCAGGCCGCGGAAAACGCGGC
GCCGGCTGCCGAAGCCGCGCTCGCCGAGGCTCAAGCCAAGATCGCCGAGTTGCAGGAGAGCTTCCTGCGCGCGAAGGCGG
AAACCGAGAACGTGCGCCGCCGCGCGCAGGACGACGTCGCGAAGGCGCACAAGTTCGCGATCGAGAGTTTCGCCGAGAAC
CTGCTGCCCGTGCTCGACAGCCTCGAGGCGGCGGTGGGCGACACGTCGGGCGATCTCGCGAAGGTCCGCGAAGGCGTCGA
GCTCACGCTGCGCCAGCTGACGAGCGCGCTCGAGAAGGGCCGCGTCGCGGCGCTCAACCCGGTCGGCGAGAAATTCGATC
CGCATCTGCACCAGGCGATCTCGATGGTGCCCGCCGACCAGGAGCCGAACACGGTCGTCGCCGTGCTGCAGAAGGGCTAC
ACGATCGCCGACCGCGTGCTGCGCCCGGCGCTCGTGACGGTCGCGCAGCCGAAGTAA

Upstream 100 bases:

>100_bases
CCCATTTGCACCGATATTGTGCGGCGCCGGCCGCTCGCGTGAACAGGCGCGGAACCGGCTGCCGTTTTGGCTTTAACCTC
TAAATCGACTTTCTAGCGAT

Downstream 100 bases:

>100_bases
GGGGCGGCGATGGCGGGCACGGGCGTCGACGTGACCGCGTTCGCGGCGTTCGACATGCAGGAGCTGGACGCCGCGAACTT
CGACGCCGGGCTCGCGAGCG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 178; Mature: 178

Protein sequence:

>178_residues
MENTQENPTDQTTEETGREAQAAENAAPAAEAALAEAQAKIAELQESFLRAKAETENVRRRAQDDVAKAHKFAIESFAEN
LLPVLDSLEAAVGDTSGDLAKVREGVELTLRQLTSALEKGRVAALNPVGEKFDPHLHQAISMVPADQEPNTVVAVLQKGY
TIADRVLRPALVTVAQPK

Sequences:

>Translated_178_residues
MENTQENPTDQTTEETGREAQAAENAAPAAEAALAEAQAKIAELQESFLRAKAETENVRRRAQDDVAKAHKFAIESFAEN
LLPVLDSLEAAVGDTSGDLAKVREGVELTLRQLTSALEKGRVAALNPVGEKFDPHLHQAISMVPADQEPNTVVAVLQKGY
TIADRVLRPALVTVAQPK
>Mature_178_residues
MENTQENPTDQTTEETGREAQAAENAAPAAEAALAEAQAKIAELQESFLRAKAETENVRRRAQDDVAKAHKFAIESFAEN
LLPVLDSLEAAVGDTSGDLAKVREGVELTLRQLTSALEKGRVAALNPVGEKFDPHLHQAISMVPADQEPNTVVAVLQKGY
TIADRVLRPALVTVAQPK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=185, Percent_Identity=36.7567567567568, Blast_Score=103, Evalue=6e-23,
Organism=Homo sapiens, GI40255109, Length=167, Percent_Identity=36.5269461077844, Blast_Score=88, Evalue=5e-18,
Organism=Escherichia coli, GI1788967, Length=152, Percent_Identity=45.3947368421053, Blast_Score=123, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17552458, Length=150, Percent_Identity=36, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324806, Length=183, Percent_Identity=31.6939890710383, Blast_Score=92, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24653432, Length=179, Percent_Identity=35.7541899441341, Blast_Score=94, Evalue=4e-20,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 19133; Mature: 19133

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENTQENPTDQTTEETGREAQAAENAAPAAEAALAEAQAKIAELQESFLRAKAETENVRR
CCCCCCCCCCCHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAQDDVAKAHKFAIESFAENLLPVLDSLEAAVGDTSGDLAKVREGVELTLRQLTSALEKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC
RVAALNPVGEKFDPHLHQAISMVPADQEPNTVVAVLQKGYTIADRVLRPALVTVAQPK
CEEEECCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MENTQENPTDQTTEETGREAQAAENAAPAAEAALAEAQAKIAELQESFLRAKAETENVRR
CCCCCCCCCCCHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAQDDVAKAHKFAIESFAENLLPVLDSLEAAVGDTSGDLAKVREGVELTLRQLTSALEKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC
RVAALNPVGEKFDPHLHQAISMVPADQEPNTVVAVLQKGYTIADRVLRPALVTVAQPK
CEEEECCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA