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The map label for this gene is rluF [H]

Identifier: 83719576

GI number: 83719576

Start: 2293064

End: 2294737

Strand: Reverse

Name: rluF [H]

Synonym: BTH_I2024

Alternate gene names: 83719576

Gene position: 2294737-2293064 (Counterclockwise)

Preceding gene: 83721536

Following gene: 83721055

Centisome position: 60.24

GC content: 73.89

Gene sequence:

>1674_bases
GTGAATATTCGAGCGTTTATGCGCACAAAATTGACCGTCAAGAATCCGCGCCCGGCCGCACCCGGCCGCGCCCCCGTCCG
TTCCGGCAGCCTCACCGCGCGCAAGGTCGCGCGGCCCGACCCGAAAGCGGCGGGCGCGAAGCCGGCTGCGGCGAAGTCCG
CAGCGCCCGCTGCCAAACCGTCGGCGCCGCGCGGCGCGGCGAGCGCCGCGCCGAAGCGCGCGCACGGGCCGTCGCGCCCA
GCCGCGCCCGAAGGCAAGCGCGTCGCAAAGCCGCGCACCACGCAGGACACCGGCCGCACGGGCGGCGAACGTGCGCCTGC
CAAGCGTGCCGGCTCGCCCGGCGCGGCGGCCGGGGAGCGTGTGCGCCGCACCGACGCGAAGCCGGAGCGGCGCGCCGCCG
ATCGCACGGCCGGCCGCGCTGCGCCGCGCGACGAGCGAGCGTCGCGCAGCTCGGATGCGCGCTCGTTCGATTCAGGCGCG
CGCGGCAAGGGCCGGTCCGAGCGCGAAGGCGCAAAACCCGGCGGCGCGCGCGGCGCGGCGGGCGCGAAGTTCGGCGGCGC
GGCGCGGACCGCGGCGCGCGGGGCAGACGCCCCAACCGGCCGCGCCCCTCGTGCGGACAGCCGCAGCGCGCGCGATGCGG
CGCCAGCGTCGTTCGCGGGCAAGCGCACGGCAGCCGGCAAGCGTGCGCCGCAGCGCACCGACGACCGCCATGGCGCATCC
GAGAAGCGCGCGCCTGCGCGCACCGAGCGCACCGCACGCTTCGACGAGCGGCCGGCAACCCGCGCGAGCGCATCCGGCGA
TCGCCGCCCCGCCGCTCGCGCGGCGACGGGCTCGCGCCCGAAGGTCGCGCAGCCGATCAAGCGCCGCATCGGCGAGCCGA
ACGAATCGGCCGCCCACATCGAGCGCGGCGATGAAACGGGCCTCGTGCGGCTGTCGAAGCGCATGTCGGAGCTTGGTCTC
TGCTCGCGCCGCGAAGCGGACGAGTGGATCGAGAAAGGCTGGGTGCTCGTCGACGGCGAGCGCATCGACACGCTCGGCAC
GAAGGTGCGCGCCGACCAGCGCATCGAGATCGATTCGAACGCGCGCGCCGAGCAGGCCGCGCAAGTGACGATCCTGCTGC
ACAAGCCGGTCGGCTACGTGTCGGGCCAGGCAGAGGACGGCTACGCGCCCGCCGCGACGCTCGTCACGCGCGAGAATCAC
TGGGGCGGCGACCGCTCGCCACTGCGCTTCTCGCCGCAGCACCTGCGCGCGCTGGCGCCCGCGGGCCGGCTCGACATCGA
TTCGACGGGCCTTCTCGTGCTGACGCAAAACGGCCGCGTCGCGAAACAGCTGATCGGCGAGCAATCCGACATCGACAAGG
AATATCTGGTGCGCGTGCGCTTCGGCGAGCGCACGACCGACATCGAACGGCACTTCCCCGCCGAGTCGCTCGCGAAGCTG
CGGCACGGCCTTGAGCTCGACGGCGTGCCGCTCAAGCCCGCGATGGTCAGCTGGCAGAACGGCGAGCAGTTGCGCTTCGT
GCTGCGCGAAGGCAAGAAGCGCCAGATTCGCCGGATGTGCGAGCTCGTCGGCCTCGAGGTGATCGGCCTGAAGCGCGTGC
GGATGGGCCGCGTGATGCTGGGCGCGTTGCCGCAAGGCCAATGGCGCTATCTCGGGCCGGACGAGTCGTTCTGA

Upstream 100 bases:

>100_bases
GCGAACGCGTCGAGGACGTCTTCATGCTCGACGGCACCGGCTTGTCCGACAACCGTCTGCAGATCCAAGTCGAAACCGAG
TTGCTGCGCGCGATCGCAGT

Downstream 100 bases:

>100_bases
GCATGGCAAGCCGGCCGCTCGCGGCCGGCCCCCGCAATCGTTGCGCCTTGAGCCCCGGCCTGCGCCGGCGGACCTCGCAC
GGAAACGCACGCGGGCGTTT

Product: pseudouridine synthase family protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MNIRAFMRTKLTVKNPRPAAPGRAPVRSGSLTARKVARPDPKAAGAKPAAAKSAAPAAKPSAPRGAASAAPKRAHGPSRP
AAPEGKRVAKPRTTQDTGRTGGERAPAKRAGSPGAAAGERVRRTDAKPERRAADRTAGRAAPRDERASRSSDARSFDSGA
RGKGRSEREGAKPGGARGAAGAKFGGAARTAARGADAPTGRAPRADSRSARDAAPASFAGKRTAAGKRAPQRTDDRHGAS
EKRAPARTERTARFDERPATRASASGDRRPAARAATGSRPKVAQPIKRRIGEPNESAAHIERGDETGLVRLSKRMSELGL
CSRREADEWIEKGWVLVDGERIDTLGTKVRADQRIEIDSNARAEQAAQVTILLHKPVGYVSGQAEDGYAPAATLVTRENH
WGGDRSPLRFSPQHLRALAPAGRLDIDSTGLLVLTQNGRVAKQLIGEQSDIDKEYLVRVRFGERTTDIERHFPAESLAKL
RHGLELDGVPLKPAMVSWQNGEQLRFVLREGKKRQIRRMCELVGLEVIGLKRVRMGRVMLGALPQGQWRYLGPDESF

Sequences:

>Translated_557_residues
MNIRAFMRTKLTVKNPRPAAPGRAPVRSGSLTARKVARPDPKAAGAKPAAAKSAAPAAKPSAPRGAASAAPKRAHGPSRP
AAPEGKRVAKPRTTQDTGRTGGERAPAKRAGSPGAAAGERVRRTDAKPERRAADRTAGRAAPRDERASRSSDARSFDSGA
RGKGRSEREGAKPGGARGAAGAKFGGAARTAARGADAPTGRAPRADSRSARDAAPASFAGKRTAAGKRAPQRTDDRHGAS
EKRAPARTERTARFDERPATRASASGDRRPAARAATGSRPKVAQPIKRRIGEPNESAAHIERGDETGLVRLSKRMSELGL
CSRREADEWIEKGWVLVDGERIDTLGTKVRADQRIEIDSNARAEQAAQVTILLHKPVGYVSGQAEDGYAPAATLVTRENH
WGGDRSPLRFSPQHLRALAPAGRLDIDSTGLLVLTQNGRVAKQLIGEQSDIDKEYLVRVRFGERTTDIERHFPAESLAKL
RHGLELDGVPLKPAMVSWQNGEQLRFVLREGKKRQIRRMCELVGLEVIGLKRVRMGRVMLGALPQGQWRYLGPDESF
>Mature_557_residues
MNIRAFMRTKLTVKNPRPAAPGRAPVRSGSLTARKVARPDPKAAGAKPAAAKSAAPAAKPSAPRGAASAAPKRAHGPSRP
AAPEGKRVAKPRTTQDTGRTGGERAPAKRAGSPGAAAGERVRRTDAKPERRAADRTAGRAAPRDERASRSSDARSFDSGA
RGKGRSEREGAKPGGARGAAGAKFGGAARTAARGADAPTGRAPRADSRSARDAAPASFAGKRTAAGKRAPQRTDDRHGAS
EKRAPARTERTARFDERPATRASASGDRRPAARAATGSRPKVAQPIKRRIGEPNESAAHIERGDETGLVRLSKRMSELGL
CSRREADEWIEKGWVLVDGERIDTLGTKVRADQRIEIDSNARAEQAAQVTILLHKPVGYVSGQAEDGYAPAATLVTRENH
WGGDRSPLRFSPQHLRALAPAGRLDIDSTGLLVLTQNGRVAKQLIGEQSDIDKEYLVRVRFGERTTDIERHFPAESLAKL
RHGLELDGVPLKPAMVSWQNGEQLRFVLREGKKRQIRRMCELVGLEVIGLKRVRMGRVMLGALPQGQWRYLGPDESF

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=249, Percent_Identity=35.3413654618474, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1787524, Length=256, Percent_Identity=32.8125, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788510, Length=252, Percent_Identity=28.5714285714286, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 59757; Mature: 59757

Theoretical pI: Translated: 11.81; Mature: 11.81

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIRAFMRTKLTVKNPRPAAPGRAPVRSGSLTARKVARPDPKAAGAKPAAAKSAAPAAKP
CCCHHEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHCCCCCCCC
SAPRGAASAAPKRAHGPSRPAAPEGKRVAKPRTTQDTGRTGGERAPAKRAGSPGAAAGER
CCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCHHHHH
VRRTDAKPERRAADRTAGRAAPRDERASRSSDARSFDSGARGKGRSEREGAKPGGARGAA
HHHCCCCHHHHHHHHHCCCCCCCHHHHHCCCCHHHCCCCCCCCCCCHHCCCCCCCCCCCC
GAKFGGAARTAARGADAPTGRAPRADSRSARDAAPASFAGKRTAAGKRAPQRTDDRHGAS
CCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCC
EKRAPARTERTARFDERPATRASASGDRRPAARAATGSRPKVAQPIKRRIGEPNESAAHI
HHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHCCCCCCCHHHH
ERGDETGLVRLSKRMSELGLCSRREADEWIEKGWVLVDGERIDTLGTKVRADQRIEIDSN
HCCCCHHHHHHHHHHHHHCCHHCCCHHHHHHCCCEEECCCCHHHHHHHHCCCCEEEECCC
ARAEQAAQVTILLHKPVGYVSGQAEDGYAPAATLVTRENHWGGDRSPLRFSPQHLRALAP
CCHHCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHCC
AGRLDIDSTGLLVLTQNGRVAKQLIGEQSDIDKEYLVRVRFGERTTDIERHFPAESLAKL
CCCCEECCCCEEEEECCCHHHHHHHCCCCCCCHHHEEEEECCCCCCHHHHHCCHHHHHHH
RHGLELDGVPLKPAMVSWQNGEQLRFVLREGKKRQIRRMCELVGLEVIGLKRVRMGRVML
HCCCCCCCCCCCCCEEECCCCHHHHHHHHCCHHHHHHHHHHHHCCHHCCHHHHHHHHHHH
GALPQGQWRYLGPDESF
HCCCCCCEEECCCCCCC
>Mature Secondary Structure
MNIRAFMRTKLTVKNPRPAAPGRAPVRSGSLTARKVARPDPKAAGAKPAAAKSAAPAAKP
CCCHHEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHCCCCCCCC
SAPRGAASAAPKRAHGPSRPAAPEGKRVAKPRTTQDTGRTGGERAPAKRAGSPGAAAGER
CCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCHHHHH
VRRTDAKPERRAADRTAGRAAPRDERASRSSDARSFDSGARGKGRSEREGAKPGGARGAA
HHHCCCCHHHHHHHHHCCCCCCCHHHHHCCCCHHHCCCCCCCCCCCHHCCCCCCCCCCCC
GAKFGGAARTAARGADAPTGRAPRADSRSARDAAPASFAGKRTAAGKRAPQRTDDRHGAS
CCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCC
EKRAPARTERTARFDERPATRASASGDRRPAARAATGSRPKVAQPIKRRIGEPNESAAHI
HHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHCCCCCCCHHHH
ERGDETGLVRLSKRMSELGLCSRREADEWIEKGWVLVDGERIDTLGTKVRADQRIEIDSN
HCCCCHHHHHHHHHHHHHCCHHCCCHHHHHHCCCEEECCCCHHHHHHHHCCCCEEEECCC
ARAEQAAQVTILLHKPVGYVSGQAEDGYAPAATLVTRENHWGGDRSPLRFSPQHLRALAP
CCHHCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHCC
AGRLDIDSTGLLVLTQNGRVAKQLIGEQSDIDKEYLVRVRFGERTTDIERHFPAESLAKL
CCCCEECCCCEEEEECCCHHHHHHHCCCCCCCHHHEEEEECCCCCCHHHHHCCHHHHHHH
RHGLELDGVPLKPAMVSWQNGEQLRFVLREGKKRQIRRMCELVGLEVIGLKRVRMGRVML
HCCCCCCCCCCCCCEEECCCCHHHHHHHHCCHHHHHHHHHHHHCCHHCCHHHHHHHHHHH
GALPQGQWRYLGPDESF
HCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]