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The map label for this gene is lysS [H]

Identifier: 83719490

GI number: 83719490

Start: 2127370

End: 2128896

Strand: Reverse

Name: lysS [H]

Synonym: BTH_I1883

Alternate gene names: 83719490

Gene position: 2128896-2127370 (Counterclockwise)

Preceding gene: 83721546

Following gene: 83720600

Centisome position: 55.89

GC content: 65.75

Gene sequence:

>1527_bases
ATGACCGAACCGATCCAACCGCAGGCCGCTGTCGCAGCGGACGAAAACCAGATCGTCGCGGAACGCCGCGACAAGCTGCG
CGCGCTGCGCGACCAAGGCATCGCCTATCCGAACGACTTCCAGCCGACGCACCACGCGGCCGATCTGCAGACCGCATACG
CGGACGCCGACAAGGAAGCGCTCGAGGCGAAGTCGCTCGAGGTCGCGATCGCCGGCCGGATGATGCTCAAGCGCGTGATG
GGCAAGGCGAGCTTCGCGACGGTGCAGGACGGCTCGGGTCAGATCCAGTTCTTCGTGACGCCCGCCGACGTCGGCGCGGA
AACGTACGATGCGTTCAAGAAGTGGGACCTGGGCGACATCGTCGCCGCGCGCGGCGTGCTGTTTCGCACGAACAAGGGCG
AGCTGTCGGTGAAGTGCACGCAGCTGCGGCTGCTCGCGAAGGCGCTGCGCCCGCTGCCCGACAAGTTCCACGGCCTCGCT
GACCAGGAAACGCGCTATCGCCAGCGCTACGTCGACCTGATCGTCACGCCCGAGACGCGCACGACGTTTCGCGCGCGCAC
GAAGGCGATCGCGTCGATCCGCAAGTTCATGGGCGACGCCGATTTCATGGAAGTCGAGACGCCGATGCTGCACCCGATCC
CGGGCGGCGCGGCCGCGAAGCCGTTCGTCACGCATCACAACGCGCTCGACATGGAAATGTTCCTGCGCATCGCGCCCGAG
CTGTACCTGAAGCGCCTCATCGTCGGCGGCTTCGAGCGCGTGTTCGAGATCAACCGGAATTTCCGCAACGAGGGCGTGTC
GCCGCGCCACAATCCGGAATTCACGATGATGGAGTTCTACGCCGCGTACACCGACTACCGCTGGCTGATGGACTTCACCG
AGCGGCTGATCCGCCAGGCGGCCGTGGACGCGCTCGGCACCGCGACGATCCAGTACCAGGGCCGCGAGCTCGATCTCGCG
CAACCGTTTCATCGCCTGACGATCACGCAGGCGATCCAGAAGTACGCGCCCAGCTACACGGATGGCCAACTGTCGGACGA
CGCGTTCCTGCGCAGCGAACTGAAGCGCCTCGGCGTCGACGTCACGCAGCCCGCGTTCCTGAACGCCGGCATCGGCGCGC
TGCAGCTCGCCCTCTTCGAGGAAACCGCGGAAGCGCAACTGTGGGAGCCGACCTTCATCATCGACTACCCGATCGAAGTG
TCGCCGCTCGCGCGCGAATCGGACACGGTGGCCGGCATCACCGAGCGTTTCGAGCTCTTCATCACCGGCCGCGAAATCGC
GAACGGGTTCTCGGAGCTGAACGATCCCGAAGACCAGGCCGCGCGCTTCAAGAAGCAGGTCGAACAGAAGGACGCGGGCG
ACGAGGAAGCGATGTTCTTCGATGCGGACTACATCCGCGCGCTCGAATACGGGATGCCTCCGACAGGCGGCTGCGGGATC
GGCATCGACCGTCTCGTGATGCTGCTGACCGACAGCCCGACGATCCGCGACGTGCTGCTGTTCCCGCACCTGCGTCGCGA
GGACTGA

Upstream 100 bases:

>100_bases
CAGCGCGAGCCTCAAACAGGGCGTGTAAGCGCCCCTCGCGCGGGCGCGGCGCCAAGCCGGCCCGCGCCCCGCTGCCGCAT
CACCCTCGAACCCGACCATC

Downstream 100 bases:

>100_bases
CGCCACGCAAACCGCCCTCCGCGCCCGCCCTTCGTTCGCTTGGGTTCGCCTCGGCCCGCTTTCGCCGGGCGGCGCGCGAT
GGAGAACGGCGGCGGGCGGC

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MTEPIQPQAAVAADENQIVAERRDKLRALRDQGIAYPNDFQPTHHAADLQTAYADADKEALEAKSLEVAIAGRMMLKRVM
GKASFATVQDGSGQIQFFVTPADVGAETYDAFKKWDLGDIVAARGVLFRTNKGELSVKCTQLRLLAKALRPLPDKFHGLA
DQETRYRQRYVDLIVTPETRTTFRARTKAIASIRKFMGDADFMEVETPMLHPIPGGAAAKPFVTHHNALDMEMFLRIAPE
LYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTERLIRQAAVDALGTATIQYQGRELDLA
QPFHRLTITQAIQKYAPSYTDGQLSDDAFLRSELKRLGVDVTQPAFLNAGIGALQLALFEETAEAQLWEPTFIIDYPIEV
SPLARESDTVAGITERFELFITGREIANGFSELNDPEDQAARFKKQVEQKDAGDEEAMFFDADYIRALEYGMPPTGGCGI
GIDRLVMLLTDSPTIRDVLLFPHLRRED

Sequences:

>Translated_508_residues
MTEPIQPQAAVAADENQIVAERRDKLRALRDQGIAYPNDFQPTHHAADLQTAYADADKEALEAKSLEVAIAGRMMLKRVM
GKASFATVQDGSGQIQFFVTPADVGAETYDAFKKWDLGDIVAARGVLFRTNKGELSVKCTQLRLLAKALRPLPDKFHGLA
DQETRYRQRYVDLIVTPETRTTFRARTKAIASIRKFMGDADFMEVETPMLHPIPGGAAAKPFVTHHNALDMEMFLRIAPE
LYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTERLIRQAAVDALGTATIQYQGRELDLA
QPFHRLTITQAIQKYAPSYTDGQLSDDAFLRSELKRLGVDVTQPAFLNAGIGALQLALFEETAEAQLWEPTFIIDYPIEV
SPLARESDTVAGITERFELFITGREIANGFSELNDPEDQAARFKKQVEQKDAGDEEAMFFDADYIRALEYGMPPTGGCGI
GIDRLVMLLTDSPTIRDVLLFPHLRRED
>Mature_507_residues
TEPIQPQAAVAADENQIVAERRDKLRALRDQGIAYPNDFQPTHHAADLQTAYADADKEALEAKSLEVAIAGRMMLKRVMG
KASFATVQDGSGQIQFFVTPADVGAETYDAFKKWDLGDIVAARGVLFRTNKGELSVKCTQLRLLAKALRPLPDKFHGLAD
QETRYRQRYVDLIVTPETRTTFRARTKAIASIRKFMGDADFMEVETPMLHPIPGGAAAKPFVTHHNALDMEMFLRIAPEL
YLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTERLIRQAAVDALGTATIQYQGRELDLAQ
PFHRLTITQAIQKYAPSYTDGQLSDDAFLRSELKRLGVDVTQPAFLNAGIGALQLALFEETAEAQLWEPTFIIDYPIEVS
PLARESDTVAGITERFELFITGREIANGFSELNDPEDQAARFKKQVEQKDAGDEEAMFFDADYIRALEYGMPPTGGCGIG
IDRLVMLLTDSPTIRDVLLFPHLRRED

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=509, Percent_Identity=39.2927308447937, Blast_Score=352, Evalue=4e-97,
Organism=Homo sapiens, GI194272210, Length=509, Percent_Identity=39.2927308447937, Blast_Score=352, Evalue=6e-97,
Organism=Homo sapiens, GI40789249, Length=327, Percent_Identity=22.6299694189602, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1789256, Length=506, Percent_Identity=57.7075098814229, Blast_Score=598, Evalue=1e-172,
Organism=Escherichia coli, GI1790571, Length=506, Percent_Identity=57.1146245059289, Blast_Score=593, Evalue=1e-170,
Organism=Escherichia coli, GI87082379, Length=326, Percent_Identity=31.9018404907975, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788173, Length=251, Percent_Identity=26.6932270916335, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=488, Percent_Identity=41.1885245901639, Blast_Score=352, Evalue=3e-97,
Organism=Caenorhabditis elegans, GI17535927, Length=488, Percent_Identity=41.1885245901639, Blast_Score=352, Evalue=4e-97,
Organism=Caenorhabditis elegans, GI71994340, Length=488, Percent_Identity=41.1885245901639, Blast_Score=351, Evalue=5e-97,
Organism=Caenorhabditis elegans, GI32566633, Length=291, Percent_Identity=24.0549828178694, Blast_Score=82, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=519, Percent_Identity=41.4258188824663, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6324256, Length=551, Percent_Identity=30.6715063520871, Blast_Score=194, Evalue=3e-50,
Organism=Drosophila melanogaster, GI24640849, Length=528, Percent_Identity=39.0151515151515, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24640851, Length=523, Percent_Identity=39.3881453154876, Blast_Score=364, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24584738, Length=289, Percent_Identity=26.2975778546713, Blast_Score=83, Evalue=6e-16,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 57234; Mature: 57103

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPIQPQAAVAADENQIVAERRDKLRALRDQGIAYPNDFQPTHHAADLQTAYADADKEA
CCCCCCCCHHEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCHHH
LEAKSLEVAIAGRMMLKRVMGKASFATVQDGSGQIQFFVTPADVGAETYDAFKKWDLGDI
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECHHCCCHHHHHHHCCCHHHH
VAARGVLFRTNKGELSVKCTQLRLLAKALRPLPDKFHGLADQETRYRQRYVDLIVTPETR
HHHCCEEEECCCCCEEEEHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHEEEEECCCCC
TTFRARTKAIASIRKFMGDADFMEVETPMLHPIPGGAAAKPFVTHHNALDMEMFLRIAPE
HHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHH
LYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTERLIRQA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVDALGTATIQYQGRELDLAQPFHRLTITQAIQKYAPSYTDGQLSDDAFLRSELKRLGVD
HHHHHCCEEEEECCCEECHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
VTQPAFLNAGIGALQLALFEETAEAQLWEPTFIIDYPIEVSPLARESDTVAGITERFELF
CCCCHHHHCCHHHHHHHHHHHHHHCEECCCEEEEECCCCCCCCCCCCCHHHHHHEEEEEE
ITGREIANGFSELNDPEDQAARFKKQVEQKDAGDEEAMFFDADYIRALEYGMPPTGGCGI
EECHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHCCCCCCCCCCC
GIDRLVMLLTDSPTIRDVLLFPHLRRED
CHHHHEEHHCCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure 
TEPIQPQAAVAADENQIVAERRDKLRALRDQGIAYPNDFQPTHHAADLQTAYADADKEA
CCCCCCCHHEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCHHH
LEAKSLEVAIAGRMMLKRVMGKASFATVQDGSGQIQFFVTPADVGAETYDAFKKWDLGDI
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECHHCCCHHHHHHHCCCHHHH
VAARGVLFRTNKGELSVKCTQLRLLAKALRPLPDKFHGLADQETRYRQRYVDLIVTPETR
HHHCCEEEECCCCCEEEEHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHEEEEECCCCC
TTFRARTKAIASIRKFMGDADFMEVETPMLHPIPGGAAAKPFVTHHNALDMEMFLRIAPE
HHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHH
LYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTERLIRQA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVDALGTATIQYQGRELDLAQPFHRLTITQAIQKYAPSYTDGQLSDDAFLRSELKRLGVD
HHHHHCCEEEEECCCEECHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
VTQPAFLNAGIGALQLALFEETAEAQLWEPTFIIDYPIEVSPLARESDTVAGITERFELF
CCCCHHHHCCHHHHHHHHHHHHHHCEECCCEEEEECCCCCCCCCCCCCHHHHHHEEEEEE
ITGREIANGFSELNDPEDQAARFKKQVEQKDAGDEEAMFFDADYIRALEYGMPPTGGCGI
EECHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHCCCCCCCCCCC
GIDRLVMLLTDSPTIRDVLLFPHLRRED
CHHHHEEHHCCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA