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The map label for this gene is ampD [H]

Identifier: 83719444

GI number: 83719444

Start: 1297333

End: 1297920

Strand: Direct

Name: ampD [H]

Synonym: BTH_I1152

Alternate gene names: 83719444

Gene position: 1297333-1297920 (Clockwise)

Preceding gene: 83718688

Following gene: 83719828

Centisome position: 34.06

GC content: 67.01

Gene sequence:

>588_bases
ATGAGCGAGGCGCGCTTCGCCGTCGATGCCGACGGCTGGGTCGACGGCGCGCGGCGCGAGCCGTCGCCGAATTTCGAAGC
GCGGCCCGACGGCGCGGCCGTTTCGCTCGTCGTGATCCACAACATCAGTCTGCCGCCGGGCGAGTTCGGCGGCGATGCGA
TCGTCGAGCTGTTCCAGAATCGTCTCGACTGCGATGCGCATCCGTACTACGCCGCGCATCTGCGCGGCGTGCGGGTTTCC
GCGCATTTCCTGATCAGGCGGGACGGCACGCTGATCCAGTTCGTGTCGTGCGGCGCGCGTGCATGGCACGCGGGCGTGTC
GAGCTTCTTCGGCCGCGAGCGTTGTAACGATTTTTCGATCGGCATCGAGCTCGAGGGTGCGGACGACGTCGCGTTCGACG
CGGCGCAATATGCGACGCTCGCCGCGCTCGCTCGCTCGCTCGCCGCGCATTACCCGATCGACGCGTTCGCAGGCCACGAG
CACGTCGCGCCCGGACGCAAGACCGATCCCGGCCCGCACTTCGACTGGCAACGTTTCGCCGACGAAGCCGGTTTTCCGTC
TCGATATTTTCCCTACCGGAAGCACTGA

Upstream 100 bases:

>100_bases
ATGCGCCGAAGAGCGACGCCGTCGCCGCGGGCGGCGAGTATTTCTGCAGTCCGGAGCACGCGGCGCGTCACGCCGCACAC
TCGGGCAGCCGCAGCGGGCA

Downstream 100 bases:

>100_bases
GTGCGGGGTCTTTTTGACGCGCGCCGCATTGTTTCGCCGGGCATGCCTTGTCGTGTCGGCGTATCGCACTTGTCAATAGA
TCGAGAGCAAATTTTTCCTT

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 195; Mature: 194

Protein sequence:

>195_residues
MSEARFAVDADGWVDGARREPSPNFEARPDGAAVSLVVIHNISLPPGEFGGDAIVELFQNRLDCDAHPYYAAHLRGVRVS
AHFLIRRDGTLIQFVSCGARAWHAGVSSFFGRERCNDFSIGIELEGADDVAFDAAQYATLAALARSLAAHYPIDAFAGHE
HVAPGRKTDPGPHFDWQRFADEAGFPSRYFPYRKH

Sequences:

>Translated_195_residues
MSEARFAVDADGWVDGARREPSPNFEARPDGAAVSLVVIHNISLPPGEFGGDAIVELFQNRLDCDAHPYYAAHLRGVRVS
AHFLIRRDGTLIQFVSCGARAWHAGVSSFFGRERCNDFSIGIELEGADDVAFDAAQYATLAALARSLAAHYPIDAFAGHE
HVAPGRKTDPGPHFDWQRFADEAGFPSRYFPYRKH
>Mature_194_residues
SEARFAVDADGWVDGARREPSPNFEARPDGAAVSLVVIHNISLPPGEFGGDAIVELFQNRLDCDAHPYYAAHLRGVRVSA
HFLIRRDGTLIQFVSCGARAWHAGVSSFFGRERCNDFSIGIELEGADDVAFDAAQYATLAALARSLAAHYPIDAFAGHEH
VAPGRKTDPGPHFDWQRFADEAGFPSRYFPYRKH

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=172, Percent_Identity=59.8837209302326, Blast_Score=198, Evalue=2e-52,
Organism=Escherichia coli, GI1787092, Length=129, Percent_Identity=39.5348837209302, Blast_Score=75, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 21457; Mature: 21326

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEARFAVDADGWVDGARREPSPNFEARPDGAAVSLVVIHNISLPPGEFGGDAIVELFQN
CCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHH
RLDCDAHPYYAAHLRGVRVSAHFLIRRDGTLIQFVSCGARAWHAGVSSFFGRERCNDFSI
HCCCCCCCCHHHHHCCEEEEEEEEEECCCCEEEEEHHCCHHHHHHHHHHHHHHCCCCEEE
GIELEGADDVAFDAAQYATLAALARSLAAHYPIDAFAGHEHVAPGRKTDPGPHFDWQRFA
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCHHHHH
DEAGFPSRYFPYRKH
HHCCCCCCCCCCCCC
>Mature Secondary Structure 
SEARFAVDADGWVDGARREPSPNFEARPDGAAVSLVVIHNISLPPGEFGGDAIVELFQN
CCCCEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHH
RLDCDAHPYYAAHLRGVRVSAHFLIRRDGTLIQFVSCGARAWHAGVSSFFGRERCNDFSI
HCCCCCCCCHHHHHCCEEEEEEEEEECCCCEEEEEHHCCHHHHHHHHHHHHHHCCCCEEE
GIELEGADDVAFDAAQYATLAALARSLAAHYPIDAFAGHEHVAPGRKTDPGPHFDWQRFA
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCHHHHH
DEAGFPSRYFPYRKH
HHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 8419294 [H]