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The map label for this gene is gyrB [H]

Identifier: 83594042

GI number: 83594042

Start: 3131976

End: 3133973

Strand: Direct

Name: gyrB [H]

Synonym: Rru_A2710

Alternate gene names: 83594042

Gene position: 3131976-3133973 (Clockwise)

Preceding gene: 83594038

Following gene: 83594045

Centisome position: 71.95

GC content: 66.17

Gene sequence:

>1998_bases
ATGTCCGACCTATTCCAAGACGCGGCGGCGGCCCAACCGGCCGACGCCTATACCGCCCGCAGCATCGAAGTGCTGGAAGG
GCTTGAACCGGTGCGTCGGCGCCCCGGCATGTACATTGGCGGAACCGACGAACGCGCCCTCCATCATCTGGTGGCGGAAA
TCCTTGATAACGCCATGGACGAGGCGGTGGCTGGCTATGCCCAGGTCATCGATATCGATCTGGCCGTCGATGGATCCGTC
TGCGTGCGCGACAACGGCCGGGGCATCCCGATCGATCCGCACCCGCGCTTCCCCGACAAAAGCGCCCTCGAGGTCATTCT
GACCACCTTGCATTCCGGCGGCAAGTTCGGCGGCGACGCCTATAAGGTTTCGGGCGGCTTGCACGGAGTCGGCATCTCGG
TGGTCAATGCGCTGTCCGACAGCCTGCGCGTCGAGGTCGCCCGCGACCGCACGCTGTGGGCCCAGGATTTCTGCCGGGGC
GCGCCGCTTGGTCCCTTGCAGAAGATCGGACCGGCGCCCAATCGCCGGGGCACCCAGGTGCGGTTCCATCCCGATGCCGA
GATCTTCGGCCCGCGCGCCCGTTTCAAACCGGCCCTGCTCCATCGCATGGCCCGGTCCAAGGCCTATCTGTTTCGCGGCG
TGCGCATCCGCTGGAGTTGCGCCCCCGAGCTGCTGCCCGATGACGCCGGCGTTCCGGCCGAGGATGTCTTCCACTTCCCC
AACGGGCTGGGGGATTACCTGGGCCACGCCCTGGCCAACCGGCCGCTGGTTGGCGGACGCACCTTCGCCGGCACCGCCAA
TCTGCCCGACGACATGGGCACGGTGGAATGGGCGCTGTGCTGGCCCGAGGACGACGAACCGTTCTTCAATTCCTACGTCA
ACACCATCCCAACCCCCGAGGGCGGCACCCATGAAGCCGGCCTGCGCAACGCCCTGACGCGCGGCTTGCGCTCCTTTGCC
GAAATGTCGGGCAATAAAAAGGGCGCGGCCGCCCTGACCGCCGAGGATGTGGTCGGCGGCGCGGCGGTGATGTTCTCGAT
GTTCCTGCGCGACCCGCAGTTCCAGGGGCAAACCAAGGAAAAACTGGCCAGCCAGGAAGCCACCCGGCTGGTCGAACAGG
CGCTGCGCGATCCCTTCGATCACTGGCTGACCGGCGCCCCCGATTCGGCCAATCTGCTGCTGACCCATGCCCTGGAGCGC
GCCGACGAACGCCTGCGCCGCCGTCAGGCCAAGGAAACCAGCCGCAAGACCGCCACCAAGAAGCTGCGCCTGCCCGGCAA
GCTGTCGGATTGCACCCGCTCGACCCGCCAGGGCACCGAATTGTTCCTGGTCGAGGGTGACAGCGCCGGCGGCTCGGCCA
AGCAGGCCCGCGACCGCCAGACCCAGGCGGTGCTGCCGTTGCGCGGCAAGATCCTCAATGTCGCCAGCGCCTCGCTCGAC
AAGCTGCGCGGCAATCAGGAACTGTCGGATCTGGCCGAGGCCCTGGGCTGCGGCACCCGGGGCGCCTTTGATCTGGAACA
TCTGCGCTACGACAAGGTGGTGATCATGACCGACGCCGATGTCGACGGCGCCCATATCGCCAGCTTGCTGATGACCTTTT
TCTTCCGCGAGATGCCAGCCCTGGTCTCGGCGGGCCATCTCTATCTGGCGATGCCGCCGCTCTACCGCCTGACCCAGGGC
GGCACCTCGGTCTATGCCCGCGACGATGCCCATAAGGACCAGTTGATGAAGACGGTCTTCAAGACCAAGGGCAAGGTCGA
GATCAGCCGCTTCAAGGGCCTGGGCGAAATGCCCCCCGCCCAATTGCGCGAAACCACCATGGCCTATGCCACCCGTCAGT
TGCTGCGGGTGACCGTGCCGGGCGGCAAGACCGAGGAAGATCGCGAAGACGCCCGCGAAACGGCGAAGCTGGTCGAGGAG
CTGATGGGCCGCAAACCCGAATTGCGCTTCCGCTTCATCCAGGAAAACGCCCGCTTCGCCGAAAACCTCGATATCTGA

Upstream 100 bases:

>100_bases
AAAAAAGCCGGGGTCGGATGACCGGCGCCGGAACGCTTTATCACCGGGGCATCGAACGGGAGGCCCTTGCCGTTGGAAGC
GGCCTGATTATAGTGGCGCC

Downstream 100 bases:

>100_bases
TCAGCCGCGCCCCGGTCCTTTATGCCAGTGCTTTGACAAAGAACAGGCTCAAGGGATCGGGGTGGTAATCGCCAAAGGGG
CCGGTTTGCGCAAAGCCGGC

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 665; Mature: 664

Protein sequence:

>665_residues
MSDLFQDAAAAQPADAYTARSIEVLEGLEPVRRRPGMYIGGTDERALHHLVAEILDNAMDEAVAGYAQVIDIDLAVDGSV
CVRDNGRGIPIDPHPRFPDKSALEVILTTLHSGGKFGGDAYKVSGGLHGVGISVVNALSDSLRVEVARDRTLWAQDFCRG
APLGPLQKIGPAPNRRGTQVRFHPDAEIFGPRARFKPALLHRMARSKAYLFRGVRIRWSCAPELLPDDAGVPAEDVFHFP
NGLGDYLGHALANRPLVGGRTFAGTANLPDDMGTVEWALCWPEDDEPFFNSYVNTIPTPEGGTHEAGLRNALTRGLRSFA
EMSGNKKGAAALTAEDVVGGAAVMFSMFLRDPQFQGQTKEKLASQEATRLVEQALRDPFDHWLTGAPDSANLLLTHALER
ADERLRRRQAKETSRKTATKKLRLPGKLSDCTRSTRQGTELFLVEGDSAGGSAKQARDRQTQAVLPLRGKILNVASASLD
KLRGNQELSDLAEALGCGTRGAFDLEHLRYDKVVIMTDADVDGAHIASLLMTFFFREMPALVSAGHLYLAMPPLYRLTQG
GTSVYARDDAHKDQLMKTVFKTKGKVEISRFKGLGEMPPAQLRETTMAYATRQLLRVTVPGGKTEEDREDARETAKLVEE
LMGRKPELRFRFIQENARFAENLDI

Sequences:

>Translated_665_residues
MSDLFQDAAAAQPADAYTARSIEVLEGLEPVRRRPGMYIGGTDERALHHLVAEILDNAMDEAVAGYAQVIDIDLAVDGSV
CVRDNGRGIPIDPHPRFPDKSALEVILTTLHSGGKFGGDAYKVSGGLHGVGISVVNALSDSLRVEVARDRTLWAQDFCRG
APLGPLQKIGPAPNRRGTQVRFHPDAEIFGPRARFKPALLHRMARSKAYLFRGVRIRWSCAPELLPDDAGVPAEDVFHFP
NGLGDYLGHALANRPLVGGRTFAGTANLPDDMGTVEWALCWPEDDEPFFNSYVNTIPTPEGGTHEAGLRNALTRGLRSFA
EMSGNKKGAAALTAEDVVGGAAVMFSMFLRDPQFQGQTKEKLASQEATRLVEQALRDPFDHWLTGAPDSANLLLTHALER
ADERLRRRQAKETSRKTATKKLRLPGKLSDCTRSTRQGTELFLVEGDSAGGSAKQARDRQTQAVLPLRGKILNVASASLD
KLRGNQELSDLAEALGCGTRGAFDLEHLRYDKVVIMTDADVDGAHIASLLMTFFFREMPALVSAGHLYLAMPPLYRLTQG
GTSVYARDDAHKDQLMKTVFKTKGKVEISRFKGLGEMPPAQLRETTMAYATRQLLRVTVPGGKTEEDREDARETAKLVEE
LMGRKPELRFRFIQENARFAENLDI
>Mature_664_residues
SDLFQDAAAAQPADAYTARSIEVLEGLEPVRRRPGMYIGGTDERALHHLVAEILDNAMDEAVAGYAQVIDIDLAVDGSVC
VRDNGRGIPIDPHPRFPDKSALEVILTTLHSGGKFGGDAYKVSGGLHGVGISVVNALSDSLRVEVARDRTLWAQDFCRGA
PLGPLQKIGPAPNRRGTQVRFHPDAEIFGPRARFKPALLHRMARSKAYLFRGVRIRWSCAPELLPDDAGVPAEDVFHFPN
GLGDYLGHALANRPLVGGRTFAGTANLPDDMGTVEWALCWPEDDEPFFNSYVNTIPTPEGGTHEAGLRNALTRGLRSFAE
MSGNKKGAAALTAEDVVGGAAVMFSMFLRDPQFQGQTKEKLASQEATRLVEQALRDPFDHWLTGAPDSANLLLTHALERA
DERLRRRQAKETSRKTATKKLRLPGKLSDCTRSTRQGTELFLVEGDSAGGSAKQARDRQTQAVLPLRGKILNVASASLDK
LRGNQELSDLAEALGCGTRGAFDLEHLRYDKVVIMTDADVDGAHIASLLMTFFFREMPALVSAGHLYLAMPPLYRLTQGG
TSVYARDDAHKDQLMKTVFKTKGKVEISRFKGLGEMPPAQLRETTMAYATRQLLRVTVPGGKTEEDREDARETAKLVEEL
MGRKPELRFRFIQENARFAENLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=625, Percent_Identity=24.8, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI19913406, Length=627, Percent_Identity=24.0829346092504, Blast_Score=129, Evalue=1e-29,
Organism=Escherichia coli, GI1789408, Length=647, Percent_Identity=43.7403400309119, Blast_Score=481, Evalue=1e-137,
Organism=Escherichia coli, GI48994957, Length=570, Percent_Identity=42.6315789473684, Blast_Score=429, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17535065, Length=581, Percent_Identity=24.0963855421687, Blast_Score=119, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI212645845, Length=556, Percent_Identity=24.8201438848921, Blast_Score=107, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI212645657, Length=162, Percent_Identity=32.0987654320988, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=644, Percent_Identity=25.6211180124224, Blast_Score=130, Evalue=7e-31,
Organism=Drosophila melanogaster, GI17136538, Length=540, Percent_Identity=25.9259259259259, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72918; Mature: 72787

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLFQDAAAAQPADAYTARSIEVLEGLEPVRRRPGMYIGGTDERALHHLVAEILDNAMD
CCHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHH
EAVAGYAQVIDIDLAVDGSVCVRDNGRGIPIDPHPRFPDKSALEVILTTLHSGGKFGGDA
HHHHHHHHEEEEEEEECCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCE
YKVSGGLHGVGISVVNALSDSLRVEVARDRTLWAQDFCRGAPLGPLQKIGPAPNRRGTQV
EEECCCCCHHHHHHHHHHHHHHEEEEHHCCHHHHHHHHCCCCCCCHHHCCCCCCCCCCEE
RFHPDAEIFGPRARFKPALLHRMARSKAYLFRGVRIRWSCAPELLPDDAGVPAEDVFHFP
EECCCHHHCCCHHHCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHCC
NGLGDYLGHALANRPLVGGRTFAGTANLPDDMGTVEWALCWPEDDEPFFNSYVNTIPTPE
CHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCC
GGTHEAGLRNALTRGLRSFAEMSGNKKGAAALTAEDVVGGAAVMFSMFLRDPQFQGQTKE
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHH
KLASQEATRLVEQALRDPFDHWLTGAPDSANLLLTHALERADERLRRRQAKETSRKTATK
HHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLRLPGKLSDCTRSTRQGTELFLVEGDSAGGSAKQARDRQTQAVLPLRGKILNVASASLD
HHCCCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHEECCCCCHHHHHHHHHHH
KLRGNQELSDLAEALGCGTRGAFDLEHLRYDKVVIMTDADVDGAHIASLLMTFFFREMPA
HHCCCCHHHHHHHHHCCCCCCCCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
LVSAGHLYLAMPPLYRLTQGGTSVYARDDAHKDQLMKTVFKTKGKVEISRFKGLGEMPPA
HHHCCCEEEECCCHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCEEHHHHCCCCCCCHH
QLRETTMAYATRQLLRVTVPGGKTEEDREDARETAKLVEELMGRKPELRFRFIQENARFA
HHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHH
ENLDI
HCCCC
>Mature Secondary Structure 
SDLFQDAAAAQPADAYTARSIEVLEGLEPVRRRPGMYIGGTDERALHHLVAEILDNAMD
CHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHH
EAVAGYAQVIDIDLAVDGSVCVRDNGRGIPIDPHPRFPDKSALEVILTTLHSGGKFGGDA
HHHHHHHHEEEEEEEECCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCE
YKVSGGLHGVGISVVNALSDSLRVEVARDRTLWAQDFCRGAPLGPLQKIGPAPNRRGTQV
EEECCCCCHHHHHHHHHHHHHHEEEEHHCCHHHHHHHHCCCCCCCHHHCCCCCCCCCCEE
RFHPDAEIFGPRARFKPALLHRMARSKAYLFRGVRIRWSCAPELLPDDAGVPAEDVFHFP
EECCCHHHCCCHHHCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHCC
NGLGDYLGHALANRPLVGGRTFAGTANLPDDMGTVEWALCWPEDDEPFFNSYVNTIPTPE
CHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCC
GGTHEAGLRNALTRGLRSFAEMSGNKKGAAALTAEDVVGGAAVMFSMFLRDPQFQGQTKE
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHH
KLASQEATRLVEQALRDPFDHWLTGAPDSANLLLTHALERADERLRRRQAKETSRKTATK
HHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLRLPGKLSDCTRSTRQGTELFLVEGDSAGGSAKQARDRQTQAVLPLRGKILNVASASLD
HHCCCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHEECCCCCHHHHHHHHHHH
KLRGNQELSDLAEALGCGTRGAFDLEHLRYDKVVIMTDADVDGAHIASLLMTFFFREMPA
HHCCCCHHHHHHHHHCCCCCCCCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
LVSAGHLYLAMPPLYRLTQGGTSVYARDDAHKDQLMKTVFKTKGKVEISRFKGLGEMPPA
HHHCCCEEEECCCHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCEEHHHHCCCCCCCHH
QLRETTMAYATRQLLRVTVPGGKTEEDREDARETAKLVEELMGRKPELRFRFIQENARFA
HHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHH
ENLDI
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9797224 [H]