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The map label for this gene is mak [H]

Identifier: 83593962

GI number: 83593962

Start: 3059652

End: 3060584

Strand: Direct

Name: mak [H]

Synonym: Rru_A2630

Alternate gene names: 83593962

Gene position: 3059652-3060584 (Clockwise)

Preceding gene: 83593960

Following gene: 83593963

Centisome position: 70.29

GC content: 69.88

Gene sequence:

>933_bases
ATGACCCTACGCCTGGGATTGGATCTTGGCGGCACCAAGACCGAGATCATCGCCCTTGATGACGAAGGACGGATCCTGCT
GCGCCGCCGCCGCCCCTCGCCGCGCGCGGCCTATGGCGCCACCCTCGACTGCCTCGCCGCCCTGGTGACCGAGGCCGAAG
CCGAATTGGGACGGCAAGGGAGCGTCGGCGTCGCCATGCCCGGCGCCATCAGCCCGGCCAGCGGCTTGGTGAAGAACGCC
AATTCCCACTGGCTGAACGGCCAGCGCCTGGACCATGACCTTGCCGAGCGCCTAGGCCGGCCGGTGCGCGTGGCCAATGA
CGCCGATTGTTTCGCCCTGTCGGAAGCCACCGACGGCGCGGCGGCCGGGGCGTCGAGCGTTTTCGGGGTGATCTTGGGCA
CCGGCGTTGGCGCCGGCATCGTCGTCAACGGCCGCCTGCTGGCCGGCCCCAATGCCATCGCCGGCGAATGGGGCCACATG
CCCCTGCCCTGGCCCGGCGACGACGAACGCCCCGGCCCCGACTGCTATTGCGGGCTGAAGGGCTGCGTCGAGACCTTTTG
TTCGGGCCCCGGACTGGCCGCCGATCACCAGAAAAGCACCGGCCACGCCATCGAAGGCCCCGCCCTGCTCGCCCTGGCCC
AAGCCGGCGACGCCCAGGCCCAGGCCAGCCTTGATCGCCATGCCGACCGTTTGGCCCGGGCCCTGGCGGTCGTCATCAAT
ATCCTCGATCCCCAGGTGATCGTCCTCGGCGGCGGCCTTGGGCAAATGCCCCATCTTTATCAGGCCCTGCCCCGGCTATG
GACGCCCTGGGTGTTCAGCGACCGCGTCGACACCCGTCTTGTCGCCCCCCGTCATGGCGATTCCAGCGGCGTTCGCGGCG
CCGCCTGGCTCTGGCCCCCCTCTTCGACACTGGAAACCGATCCCCATGCGTGA

Upstream 100 bases:

>100_bases
GCAAGACCGCACAAGGATCGGCCGTTCTGTTTAGGGAGCCGTTAATCCGCCGACGCCCGCTTTGCCCGTTCCATTTTTTC
CAAAAAGGAGTCCCGTGCCG

Downstream 100 bases:

>100_bases
TGACTACTTCTACGAGACGGCAAGCGGTCACGGCCTGCCCCATGATCCGATCAAGGCCATCGTCGCCCCGCGACCCATCG
GCTGGATTTCGACCATCGAT

Product: N-acetylglucosamine kinase

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MTLRLGLDLGGTKTEIIALDDEGRILLRRRRPSPRAAYGATLDCLAALVTEAEAELGRQGSVGVAMPGAISPASGLVKNA
NSHWLNGQRLDHDLAERLGRPVRVANDADCFALSEATDGAAAGASSVFGVILGTGVGAGIVVNGRLLAGPNAIAGEWGHM
PLPWPGDDERPGPDCYCGLKGCVETFCSGPGLAADHQKSTGHAIEGPALLALAQAGDAQAQASLDRHADRLARALAVVIN
ILDPQVIVLGGGLGQMPHLYQALPRLWTPWVFSDRVDTRLVAPRHGDSSGVRGAAWLWPPSSTLETDPHA

Sequences:

>Translated_310_residues
MTLRLGLDLGGTKTEIIALDDEGRILLRRRRPSPRAAYGATLDCLAALVTEAEAELGRQGSVGVAMPGAISPASGLVKNA
NSHWLNGQRLDHDLAERLGRPVRVANDADCFALSEATDGAAAGASSVFGVILGTGVGAGIVVNGRLLAGPNAIAGEWGHM
PLPWPGDDERPGPDCYCGLKGCVETFCSGPGLAADHQKSTGHAIEGPALLALAQAGDAQAQASLDRHADRLARALAVVIN
ILDPQVIVLGGGLGQMPHLYQALPRLWTPWVFSDRVDTRLVAPRHGDSSGVRGAAWLWPPSSTLETDPHA
>Mature_309_residues
TLRLGLDLGGTKTEIIALDDEGRILLRRRRPSPRAAYGATLDCLAALVTEAEAELGRQGSVGVAMPGAISPASGLVKNAN
SHWLNGQRLDHDLAERLGRPVRVANDADCFALSEATDGAAAGASSVFGVILGTGVGAGIVVNGRLLAGPNAIAGEWGHMP
LPWPGDDERPGPDCYCGLKGCVETFCSGPGLAADHQKSTGHAIEGPALLALAQAGDAQAQASLDRHADRLARALAVVINI
LDPQVIVLGGGLGQMPHLYQALPRLWTPWVFSDRVDTRLVAPRHGDSSGVRGAAWLWPPSSTLETDPHA

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Homo sapiens, GI298566325, Length=271, Percent_Identity=27.6752767527675, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI298566315, Length=270, Percent_Identity=28.1481481481481, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI4885285, Length=271, Percent_Identity=28.0442804428044, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI190014632, Length=271, Percent_Identity=28.0442804428044, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI87081733, Length=300, Percent_Identity=54.6666666666667, Blast_Score=331, Evalue=3e-92,
Organism=Escherichia coli, GI1787363, Length=303, Percent_Identity=39.6039603960396, Blast_Score=205, Evalue=3e-54,
Organism=Escherichia coli, GI1786891, Length=267, Percent_Identity=26.2172284644195, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787878, Length=214, Percent_Identity=28.9719626168224, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87082230, Length=269, Percent_Identity=34.9442379182156, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790522, Length=304, Percent_Identity=26.9736842105263, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 32272; Mature: 32141

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRLGLDLGGTKTEIIALDDEGRILLRRRRPSPRAAYGATLDCLAALVTEAEAELGRQG
CEEEEECCCCCCCEEEEEECCCCCEEEEECCCCCCHHHCCHHHHHHHHHHHHHHHHCCCC
SVGVAMPGAISPASGLVKNANSHWLNGQRLDHDLAERLGRPVRVANDADCFALSEATDGA
CCCEECCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHCCCCEEECCCCCEEEECCCCCCC
AAGASSVFGVILGTGVGAGIVVNGRLLAGPNAIAGEWGHMPLPWPGDDERPGPDCYCGLK
CCCHHHHHHHHHHCCCCCCEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHCHH
GCVETFCSGPGLAADHQKSTGHAIEGPALLALAQAGDAQAQASLDRHADRLARALAVVIN
HHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
ILDPQVIVLGGGLGQMPHLYQALPRLWTPWVFSDRVDTRLVAPRHGDSSGVRGAAWLWPP
HCCCEEEEEECCCCCCCHHHHHHHHHCCCCEECCCCCCEEECCCCCCCCCCCCEEEECCC
SSTLETDPHA
CCCCCCCCCC
>Mature Secondary Structure 
TLRLGLDLGGTKTEIIALDDEGRILLRRRRPSPRAAYGATLDCLAALVTEAEAELGRQG
EEEEECCCCCCCEEEEEECCCCCEEEEECCCCCCHHHCCHHHHHHHHHHHHHHHHCCCC
SVGVAMPGAISPASGLVKNANSHWLNGQRLDHDLAERLGRPVRVANDADCFALSEATDGA
CCCEECCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHCCCCEEECCCCCEEEECCCCCCC
AAGASSVFGVILGTGVGAGIVVNGRLLAGPNAIAGEWGHMPLPWPGDDERPGPDCYCGLK
CCCHHHHHHHHHHCCCCCCEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHCHH
GCVETFCSGPGLAADHQKSTGHAIEGPALLALAQAGDAQAQASLDRHADRLARALAVVIN
HHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
ILDPQVIVLGGGLGQMPHLYQALPRLWTPWVFSDRVDTRLVAPRHGDSSGVRGAAWLWPP
HCCCEEEEEECCCCCCCHHHHHHHHHCCCCEECCCCCCEEECCCCCCCCCCCCEEEECCC
SSTLETDPHA
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]