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The map label for this gene is proW [H]

Identifier: 83593811

GI number: 83593811

Start: 2876437

End: 2877510

Strand: Direct

Name: proW [H]

Synonym: Rru_A2476

Alternate gene names: 83593811

Gene position: 2876437-2877510 (Clockwise)

Preceding gene: 83593810

Following gene: 83593812

Centisome position: 66.08

GC content: 66.67

Gene sequence:

>1074_bases
ATGGCTGACCCCGCGAGCGACCCCTGGGGCGCGGCCCCGCCCTCCCCCGCCGAAACGCCGGCCGGCGATTGGCTGGGGGG
CGCCTCCGCTCCGCCGCCGGCCTTCGATTGGGCCGACCCCTTCGCCCGGCCGCTGATTCCGCTCGACGACTGGACCAATC
AGGGGCTCGACTGGCTGGTCTTGTCCTTCCGCCCGCTCTTCCAGGCGATCCGCTGGCCCATCGACGCCGTGCTGACGGCG
CTCAACGGCACGCTCGTCGGTGTGCCGCCCCCCGTCATGCTGGCGATCATCGCCCTCCTCGCTTGGCAAATCGCCGGGCG
CAAGATGGCGATCGGCTCTTTGATCTCGATGACTCTCATCGGTCTGATCGGCGCCTGGAGCGAAGCGATGCTGACCTTGT
CGCTGGTCTTGACCTCGGTGATGTTCTGCATCGCCCTCGGCCTGCCCTCGGGCATCCTGATGGCGCGCAGCGACCGGCTG
GCCAAACTCTCGCGGCCTTTGCTCGATGCCATGCAGACGACCCCGGCCTTCGTCTATCTGGTGCCCATCGTCATGCTGTT
TGGCATCGGCAATGTGCCCGGGGTGGTGGTGACGATCATCTTCGCCCTGCCGCCCTTGATCCGCCTGACCAATCTGGGGA
TTAGGCAGGTTCCCGAAGATCTGGTCGAAGCCGCGCGCAGTTTCGGCGCCTCGAAAACCCAGTTGCTGACCAAGGTGCAA
CTGCCGCTGGCCCTGCCGACGATCATGGCGGGCATCAACCAAACGCTGATGCTGGCGCTGTCGATGGTGGTGATCGCCTC
GATGATCGCCGTTGGCGGGCTGGGGCAAATGGTTCTGCGCGGCATCGGCCGGCTTGATATGGGGCTGGCCACCGTTGGCG
GCGTCGCCATCGTCTTGCTGGCCATTCTGATCGATCGCCTGACCCAGTCGATGGGCGTCGCCAGCGCCGCCCAGGCCAAG
GCACCCTGGTACACCAGCGGTCCCGTCGGACTGGTCGTGCGTCTGGTGTCCCTGCGCAAGGGCACCGCCCACCCCGCTCG
CGAAAGCCTGCCAAAAGGAGAACCGATCTCATGA

Upstream 100 bases:

>100_bases
GCGCCTTGCGGTCTTCCCGTCATCGACGACGCCGGTCGCTACCTTGGCGTCATCTCGCGGGCCCTTCTTCTTGAAACCCT
CGACCGGGAGACCCTGACCA

Downstream 100 bases:

>100_bases
TCTTGCCCGCCGCGCCGCGCCTCCGCTTTCTCGCCCCCGTGTCGGCCCCCGTTCTGGCGGTTGTCTGCGCCCTTGGCGTC
TTGCCGGCAAACGCCGCCGA

Product: binding-protein dependent transport system inner membrane protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MADPASDPWGAAPPSPAETPAGDWLGGASAPPPAFDWADPFARPLIPLDDWTNQGLDWLVLSFRPLFQAIRWPIDAVLTA
LNGTLVGVPPPVMLAIIALLAWQIAGRKMAIGSLISMTLIGLIGAWSEAMLTLSLVLTSVMFCIALGLPSGILMARSDRL
AKLSRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPLIRLTNLGIRQVPEDLVEAARSFGASKTQLLTKVQ
LPLALPTIMAGINQTLMLALSMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVAIVLLAILIDRLTQSMGVASAAQAK
APWYTSGPVGLVVRLVSLRKGTAHPARESLPKGEPIS

Sequences:

>Translated_357_residues
MADPASDPWGAAPPSPAETPAGDWLGGASAPPPAFDWADPFARPLIPLDDWTNQGLDWLVLSFRPLFQAIRWPIDAVLTA
LNGTLVGVPPPVMLAIIALLAWQIAGRKMAIGSLISMTLIGLIGAWSEAMLTLSLVLTSVMFCIALGLPSGILMARSDRL
AKLSRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPLIRLTNLGIRQVPEDLVEAARSFGASKTQLLTKVQ
LPLALPTIMAGINQTLMLALSMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVAIVLLAILIDRLTQSMGVASAAQAK
APWYTSGPVGLVVRLVSLRKGTAHPARESLPKGEPIS
>Mature_356_residues
ADPASDPWGAAPPSPAETPAGDWLGGASAPPPAFDWADPFARPLIPLDDWTNQGLDWLVLSFRPLFQAIRWPIDAVLTAL
NGTLVGVPPPVMLAIIALLAWQIAGRKMAIGSLISMTLIGLIGAWSEAMLTLSLVLTSVMFCIALGLPSGILMARSDRLA
KLSRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPLIRLTNLGIRQVPEDLVEAARSFGASKTQLLTKVQL
PLALPTIMAGINQTLMLALSMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVAIVLLAILIDRLTQSMGVASAAQAKA
PWYTSGPVGLVVRLVSLRKGTAHPARESLPKGEPIS

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine/L-proline [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=327, Percent_Identity=65.7492354740061, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1788451, Length=191, Percent_Identity=30.8900523560209, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788449, Length=190, Percent_Identity=28.4210526315789, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786564, Length=154, Percent_Identity=30.5194805194805, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 37456; Mature: 37325

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADPASDPWGAAPPSPAETPAGDWLGGASAPPPAFDWADPFARPLIPLDDWTNQGLDWLV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHH
LSFRPLFQAIRWPIDAVLTALNGTLVGVPPPVMLAIIALLAWQIAGRKMAIGSLISMTLI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GLIGAWSEAMLTLSLVLTSVMFCIALGLPSGILMARSDRLAKLSRPLLDAMQTTPAFVYL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VPIVMLFGIGNVPGVVVTIIFALPPLIRLTNLGIRQVPEDLVEAARSFGASKTQLLTKVQ
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHCCCHHHHHHHHHH
LPLALPTIMAGINQTLMLALSMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVAIVLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHHH
AILIDRLTQSMGVASAAQAKAPWYTSGPVGLVVRLVSLRKGTAHPARESLPKGEPIS
HHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCC
>Mature Secondary Structure 
ADPASDPWGAAPPSPAETPAGDWLGGASAPPPAFDWADPFARPLIPLDDWTNQGLDWLV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHH
LSFRPLFQAIRWPIDAVLTALNGTLVGVPPPVMLAIIALLAWQIAGRKMAIGSLISMTLI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GLIGAWSEAMLTLSLVLTSVMFCIALGLPSGILMARSDRLAKLSRPLLDAMQTTPAFVYL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VPIVMLFGIGNVPGVVVTIIFALPPLIRLTNLGIRQVPEDLVEAARSFGASKTQLLTKVQ
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHCCCHHHHHHHHHH
LPLALPTIMAGINQTLMLALSMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVAIVLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHHH
AILIDRLTQSMGVASAAQAKAPWYTSGPVGLVVRLVSLRKGTAHPARESLPKGEPIS
HHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2649479; 9205837; 9278503 [H]