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The map label for this gene is cysD

Identifier: 83593624

GI number: 83593624

Start: 2647248

End: 2648138

Strand: Direct

Name: cysD

Synonym: Rru_A2289

Alternate gene names: 83593624

Gene position: 2647248-2648138 (Clockwise)

Preceding gene: 83593613

Following gene: 83593625

Centisome position: 60.82

GC content: 64.09

Gene sequence:

>891_bases
GTGAAGCACCTGGACTCCCTGGAAGCCGAAAGCATCCACATCCTGCGCGAAACGGCCGCCCAGTTCCGCAAGCCGGTCTT
GATGTATTCGATCGGCAAGGACAGCTCGGTTCTGCTGCATCTGGCGCGCAAGGCGTTCTATCCCTCGCGCCCGCCCTTTC
CCTTTCTGCACATCGACACCACGTGGAAATTCCACGAGATGATCGCCTTTCGCGACAAGCGCGTCGCCGAGGAGGGGGTG
GATCTGATCGTCCACACCAACCGCGCCGGGGTCGAAGCCGGGGTCAGCCCCTTCACCCATGGCGCGGCCTATACCGACAT
CATGAAGACCCAGGCCCTGCGCGAGGCGCTGACCCAGGGCGGCTTCGATGGCGTCATCGGCGGGGCGCGGCGCGACGAGG
AGAAATCCCGGGCCAAGGAGCGGGTCTTCAGCTTCCGCGACGCCCATCACCGCTGGGACCCCAAGAACCAGCGTCCCGAA
TTGTGGAACACCTATAACGGCCGGGTCGGCAAGGGCGAAAGCGTGCGGGTCTTCCCGCTGTCGAATTGGACCGAGCTTGA
TATCTGGCTGTATATCCTGCGCGAATCGATCCCGGTGGTGCCGCTTTATCTGGCGAAGCCCCGGCCGGTGGTCGAACGCG
ACGGCGCCCTGATCATGGTCGATGACAACCGCCTGCCCCTTGAACCGGGCGAAACGCCGCAAACCCGCTGGGTCCGCTTC
CGCACGCTCGGCTGTTACCCGCTGACCGGGGCGATCGAAAGCCGAGCGGCCTCGCTGCCCGAGATCATCGCCGAAATGCT
GGTGGCCCGCACCTCCGAACGCCAGGGGCGGCTGATCGACCAGGATCAGGCCGCATCGATGGAAATGAAAAAGCAGGAAG
GATACTTCTGA

Upstream 100 bases:

>100_bases
TGGTGGCGGGCGGGGGATTGATGTACGACACTATTCCACACTTCGTTTGGACTTTAATTTTAAGTTTAACAATTTAAATA
CGTGAATAGGATCTTTTGTC

Downstream 100 bases:

>100_bases
TGGACGGCGTCAGCGACGCGGCCCGCCAGGTCGAAAACTATCTGGCCCACCACGAGGACAAGGGGTTGTTGCGCCTGCTC
ACCTGCGGCAGCGTCGACGA

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MKHLDSLEAESIHILRETAAQFRKPVLMYSIGKDSSVLLHLARKAFYPSRPPFPFLHIDTTWKFHEMIAFRDKRVAEEGV
DLIVHTNRAGVEAGVSPFTHGAAYTDIMKTQALREALTQGGFDGVIGGARRDEEKSRAKERVFSFRDAHHRWDPKNQRPE
LWNTYNGRVGKGESVRVFPLSNWTELDIWLYILRESIPVVPLYLAKPRPVVERDGALIMVDDNRLPLEPGETPQTRWVRF
RTLGCYPLTGAIESRAASLPEIIAEMLVARTSERQGRLIDQDQAASMEMKKQEGYF

Sequences:

>Translated_296_residues
MKHLDSLEAESIHILRETAAQFRKPVLMYSIGKDSSVLLHLARKAFYPSRPPFPFLHIDTTWKFHEMIAFRDKRVAEEGV
DLIVHTNRAGVEAGVSPFTHGAAYTDIMKTQALREALTQGGFDGVIGGARRDEEKSRAKERVFSFRDAHHRWDPKNQRPE
LWNTYNGRVGKGESVRVFPLSNWTELDIWLYILRESIPVVPLYLAKPRPVVERDGALIMVDDNRLPLEPGETPQTRWVRF
RTLGCYPLTGAIESRAASLPEIIAEMLVARTSERQGRLIDQDQAASMEMKKQEGYF
>Mature_296_residues
MKHLDSLEAESIHILRETAAQFRKPVLMYSIGKDSSVLLHLARKAFYPSRPPFPFLHIDTTWKFHEMIAFRDKRVAEEGV
DLIVHTNRAGVEAGVSPFTHGAAYTDIMKTQALREALTQGGFDGVIGGARRDEEKSRAKERVFSFRDAHHRWDPKNQRPE
LWNTYNGRVGKGESVRVFPLSNWTELDIWLYILRESIPVVPLYLAKPRPVVERDGALIMVDDNRLPLEPGETPQTRWVRF
RTLGCYPLTGAIESRAASLPEIIAEMLVARTSERQGRLIDQDQAASMEMKKQEGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily

Homologues:

Organism=Escherichia coli, GI1789109, Length=297, Percent_Identity=67.6767676767677, Blast_Score=435, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSD_RHORT (Q2RS06)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_427376.1
- ProteinModelPortal:   Q2RS06
- SMR:   Q2RS06
- STRING:   Q2RS06
- GeneID:   3835717
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A2289
- NMPDR:   fig|1085.1.peg.3765
- eggNOG:   COG0175
- HOGENOM:   HBG758022
- OMA:   SNAVHET
- PhylomeDB:   Q2RS06
- ProtClustDB:   PRK05253
- BioCyc:   RRUB269796:RRU_A2289-MONOMER
- HAMAP:   MF_00064
- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF002936
- TIGRFAMs:   TIGR02039

Pfam domain/function: PF01507 PAPS_reduct

EC number: =2.7.7.4

Molecular weight: Translated: 33842; Mature: 33842

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLDSLEAESIHILRETAAQFRKPVLMYSIGKDSSVLLHLARKAFYPSRPPFPFLHIDT
CCCCHHCCHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEEECC
TWKFHEMIAFRDKRVAEEGVDLIVHTNRAGVEAGVSPFTHGAAYTDIMKTQALREALTQG
CHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
GFDGVIGGARRDEEKSRAKERVFSFRDAHHRWDPKNQRPELWNTYNGRVGKGESVRVFPL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEC
SNWTELDIWLYILRESIPVVPLYLAKPRPVVERDGALIMVDDNRLPLEPGETPQTRWVRF
CCCCHHHHHHHHHHHCCCEEEEEECCCCCCEECCCEEEEEECCCCCCCCCCCCCHHEEEE
RTLGCYPLTGAIESRAASLPEIIAEMLVARTSERQGRLIDQDQAASMEMKKQEGYF
EEECCEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKHLDSLEAESIHILRETAAQFRKPVLMYSIGKDSSVLLHLARKAFYPSRPPFPFLHIDT
CCCCHHCCHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEEECC
TWKFHEMIAFRDKRVAEEGVDLIVHTNRAGVEAGVSPFTHGAAYTDIMKTQALREALTQG
CHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
GFDGVIGGARRDEEKSRAKERVFSFRDAHHRWDPKNQRPELWNTYNGRVGKGESVRVFPL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEC
SNWTELDIWLYILRESIPVVPLYLAKPRPVVERDGALIMVDDNRLPLEPGETPQTRWVRF
CCCCHHHHHHHHHHHCCCEEEEEECCCCCCEECCCEEEEEECCCCCCCCCCCCCHHEEEE
RTLGCYPLTGAIESRAASLPEIIAEMLVARTSERQGRLIDQDQAASMEMKKQEGYF
EEECCEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA