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The map label for this gene is recN [H]

Identifier: 82751124

GI number: 82751124

Start: 1515551

End: 1517230

Strand: Reverse

Name: recN [H]

Synonym: SAB1392c

Alternate gene names: 82751124

Gene position: 1517230-1515551 (Counterclockwise)

Preceding gene: 82751125

Following gene: 82751123

Centisome position: 55.32

GC content: 30.89

Gene sequence:

>1680_bases
ATGTTACAAACCTTATCAATCAAGCAATTTGCTATTATTGAAGAGTTAGAAATTCAATTTTCTGATGGTTTAACTGTTTT
AAGTGGTGAAACGGGTTCAGGTAAGTCAATTATTATAGATGCAATTGGACAATTAATTGGAATGAGAGCTTCATCTGATT
TTGTAAGACATGGTGAAAAAAAGGCTGTCATCGAAGGTATATTTGATATTGACGAGAGTAAAGATGCTATACATATATTG
GAAAATATGGATATTGATGTAGATGAAGATTTTTTATTAGTTAAACGGGAAATTTTTAGCTCTGGTAAAAGTCTTTGTAA
AATTAATAATCAAACAGTTACTTTACAAGATTTACGAAAAGTTATGCAAGAATTATTAGATATTCATGGCCAACATGAAA
CGCAGTCTTTGTTGAAACAAAAATACCATTTAACATTATTGGATAATTACGCAGAATCACGTTATCAAGATTTGTTGGAT
AAATATCACCAAACTTTTCAAAATTATAAAGCCAAAAAGAAAGAGTTAGAAGATTTAGAATCAGCAGACCAAGCATTGCT
GCAGCGTTTAGATTTAATGAAATTTCAATTAGAAGAATTGTCTGAAGCACATCTAAAAGAAGGCGAAATAGAGCAACTTG
AAATAGATATTAAACGCATTCAAAACTCTGAAAAATTAAGCTTAGCACTTAATAACGCACATATGACATTAACGGATGAA
AATGCTATCACTGATCGTTTGTATGAACTAAGCAACCATTTGTTAACAATAAACGATATAGTTCCAAACAAATATGACAA
ATTAAAAGAAGATATTGATCAATTTTATTACATTTTAGAAGATGCAAAACATGAGTTATACGATGAAATGGCTAATACAG
AATTCGACGAACAAGTGTTAAACGAGTATGAATCTCGTATGAATTTGCTGAATAATTTAAAACGCAAATATGGAAAAGAT
ATTTCAGAATTAATCGCATATCAAGAAAAACTTAATAATGAAATCAATAAAATTGAAAACTATGAACAAAGTACATCGCA
GTTACGAGAAGAAATAAATGCATTGTATAATCAAGTTATAGAGGTTGGACAAGCGCTATCAAAGCAGCGTCGTATAATCG
CTAGGGAGTTGAGAGACCACATTGTATCGGAAATTCAAAACTTACAAATGAAAGACGCAAATCTTGAGATTTCATTTAAA
AAATTAGAAGAACCGAATATTGATGGAATCGAATTTGTAGAATTTTTAATCAGTCCTAATAAAGGGGAACCATTAAAAAG
TTTAAATAAAATTGCGTCAGGTGGAGAACTTTCTAGAATTATGTTAGCGTTAAAAAGTATTTTTGTTAAATCGAGAGGTC
AAACTGCAATTTTATTCGATGAGGTTGACTCAGGTGTATCTGGACAAGCTGCACAAAAAATGGCAGAGAAAATGCGAGAT
ATAGCAGAATACATACAAGTTATTTGTATTTCTCACTTGCCACAAGTTGCATCGATGAGCGACCATCATTTATTAATTTC
GAAATCATCAAAAGATGATCGTACAACAACACAAGTTCAAGAGTTAATTGGCGATGATAAAGTAGATGAAATTGCACGAA
TGATTTCAGGTGCAAGTGTTACTGATTTAACTCGAGAAAATGCAAGAGAAATGATACAACACAATCAAAGACGTAGATAA

Upstream 100 bases:

>100_bases
TTGTGGTGATGATACTTGTTTAATTATTTGTCGAAGCAAAGAGGCAAGTGATGAAATCAAGTCAAGAATTTTCAATTTGT
TATAAGGATGCGATGAGTTT

Downstream 100 bases:

>100_bases
TTTCCAAAGTGTGTTGAATACTCATTTATGGATTTATTTATGTGCATTCAAACTAAATAGACAATCATGTAAAATAGGTA
ATTAATGGACATGAAAACTA

Product: DNA repair protein

Products: NA

Alternate protein names: Recombination protein N [H]

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQLIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHIL
ENMDIDVDEDFLLVKREIFSSGKSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLD
KYHQTFQNYKAKKKELEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIKRIQNSEKLSLALNNAHMTLTDE
NAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFYYILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKD
ISELIAYQEKLNNEINKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIIARELRDHIVSEIQNLQMKDANLEISFK
KLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVKSRGQTAILFDEVDSGVSGQAAQKMAEKMRD
IAEYIQVICISHLPQVASMSDHHLLISKSSKDDRTTTQVQELIGDDKVDEIARMISGASVTDLTRENAREMIQHNQRRR

Sequences:

>Translated_559_residues
MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQLIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHIL
ENMDIDVDEDFLLVKREIFSSGKSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLD
KYHQTFQNYKAKKKELEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIKRIQNSEKLSLALNNAHMTLTDE
NAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFYYILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKD
ISELIAYQEKLNNEINKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIIARELRDHIVSEIQNLQMKDANLEISFK
KLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVKSRGQTAILFDEVDSGVSGQAAQKMAEKMRD
IAEYIQVICISHLPQVASMSDHHLLISKSSKDDRTTTQVQELIGDDKVDEIARMISGASVTDLTRENAREMIQHNQRRR
>Mature_559_residues
MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQLIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHIL
ENMDIDVDEDFLLVKREIFSSGKSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLD
KYHQTFQNYKAKKKELEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIKRIQNSEKLSLALNNAHMTLTDE
NAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFYYILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKD
ISELIAYQEKLNNEINKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIIARELRDHIVSEIQNLQMKDANLEISFK
KLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVKSRGQTAILFDEVDSGVSGQAAQKMAEKMRD
IAEYIQVICISHLPQVASMSDHHLLISKSSKDDRTTTQVQELIGDDKVDEIARMISGASVTDLTRENAREMIQHNQRRR

Specific function: Involved in recombinational repair of damaged DNA. Seems to be the first protein recruited to repair centers, foci that are the site of double-strand DNA break(s), followed by recO and then recF [H]

COG id: COG0497

COG function: function code L; ATPase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recN family [H]

Homologues:

Organism=Escherichia coli, GI48994901, Length=558, Percent_Identity=34.2293906810036, Blast_Score=290, Evalue=1e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004604
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 64282; Mature: 64282

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQLIGMRASSDFVRHGEK
CCCCCCHHHHHHHHHHHHEECCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHCCCH
KAVIEGIFDIDESKDAIHILENMDIDVDEDFLLVKREIFSSGKSLCKINNQTVTLQDLRK
HHHHHHHHCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHEEECCCEEEHHHHHH
VMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLDKYHQTFQNYKAKKKELEDLE
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIKRIQNSEKLSLALNNAHMTLTDE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEECCEEEECCC
NAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFYYILEDAKHELYDEMANTEFDEQVL
HHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
NEYESRMNLLNNLKRKYGKDISELIAYQEKLNNEINKIENYEQSTSQLREEINALYNQVI
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVGQALSKQRRIIARELRDHIVSEIQNLQMKDANLEISFKKLEEPNIDGIEFVEFLISPN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHCCCCCCCCHHHHHHHHHCCC
KGEPLKSLNKIASGGELSRIMLALKSIFVKSRGQTAILFDEVDSGVSGQAAQKMAEKMRD
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
IAEYIQVICISHLPQVASMSDHHLLISKSSKDDRTTTQVQELIGDDKVDEIARMISGASV
HHHHHHHHHHHHCCHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCH
TDLTRENAREMIQHNQRRR
HHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQLIGMRASSDFVRHGEK
CCCCCCHHHHHHHHHHHHEECCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHCCCH
KAVIEGIFDIDESKDAIHILENMDIDVDEDFLLVKREIFSSGKSLCKINNQTVTLQDLRK
HHHHHHHHCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHEEECCCEEEHHHHHH
VMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLDKYHQTFQNYKAKKKELEDLE
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIKRIQNSEKLSLALNNAHMTLTDE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEECCEEEECCC
NAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFYYILEDAKHELYDEMANTEFDEQVL
HHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
NEYESRMNLLNNLKRKYGKDISELIAYQEKLNNEINKIENYEQSTSQLREEINALYNQVI
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVGQALSKQRRIIARELRDHIVSEIQNLQMKDANLEISFKKLEEPNIDGIEFVEFLISPN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHCCCCCCCCHHHHHHHHHCCC
KGEPLKSLNKIASGGELSRIMLALKSIFVKSRGQTAILFDEVDSGVSGQAAQKMAEKMRD
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
IAEYIQVICISHLPQVASMSDHHLLISKSSKDDRTTTQVQELIGDDKVDEIARMISGASV
HHHHHHHHHHHHCCHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCH
TDLTRENAREMIQHNQRRR
HHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 2507400; 9044256 [H]