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The map label for this gene is ypdA [H]

Identifier: 82751076

GI number: 82751076

Start: 1470190

End: 1471317

Strand: Reverse

Name: ypdA [H]

Synonym: SAB1342c

Alternate gene names: 82751076

Gene position: 1471317-1470190 (Counterclockwise)

Preceding gene: 82751077

Following gene: 82751074

Centisome position: 53.65

GC content: 33.42

Gene sequence:

>1128_bases
GTGTTATTCAACTCCGTAGCTAACAATTCTCTATGCACATTAAACAAATTGTCAAAAATATATCATAAATCTTCAAGCAC
AGTCTTAGCGCATCAATCACTGAACTGTTATAATAGTTTGGGATTAAAGGAGGCCGAAACAATGCAAAAAGTTGAAAGTA
TCATAATTGGTGGAGGGCCATGCGGATTAAGTGTGGCTATTGAACAAAAAAGAAAAGGTATTGATACCTTAATTATTGAA
AAGGGTAATGTCGTTGAATCAATCTACAATTATCCTACTCACCAAACATTTTTCTCATCAAGTGATAAATTAAGTATTGG
GGACGTGCCGTTTATCGTTGAAGAAAGTAAACCAAGACGTAATCAAGCGCTAGTTTATTACCGAGAAGTTGTAAAACATC
ATCAATTAAAAGTAAATGCATTTGAAGAAGTATTAACTGTTAAAAAAATGAATAATAAATTTACTATTACTACGACGAAA
GATGTTTATGAATGTCGATTTTTAACAATCGCGACAGGCTATTATGGTCAGCATAATACATTAGAAGTTGAAGGTGCGGA
TTTACCTAAAGTGTTCCATTATTTTAAAGAAGCACATCCGTATTTTGATCAAGATGTTGTTATTATTGGTGGTAAGAATT
CGGCTATCGATGCTGCTTTGGAGTTGGAAAAAGCTGGTGCTAACGTGACGGTTCTATATCGTGGTGGAGATTATTCGCCT
TCAATTAAACCTTGGATACTTCCAAATTTCACAGCATTAGTAAATCATGAAAAAATTGACATGGAATTTAATGCTAATGT
TACCCAAATAACTGAAGATACTGTGACTTATGAAGTAAATGGTGAAAGTAAAACAATACACAATGATTATGTATTTGCGA
TGATTGGTTATCATCCCGATTATGAATTTTTAAAATCTGTAGGCATTCAAATTAATACAAATGAATTTGGAACAGCGCCT
ATGTATAATAAAGAAACATACGAAACAAATATCGAAAATTGCTACATTGCAGGTGTGATTGCTGCAGGGAACGATGCGAA
TACCATTTTTATTGAAAATGGTAAATTCCACGGTGGCATTATTGCTCAAAGCATGCTAGCTAAGAAACAAACGCCGTTAG
AATCATAA

Upstream 100 bases:

>100_bases
TATCAATTAAACTTTTTACATAGTCATCAAAAAGATAAAACACCACAATCAACAAATTTAACGAGGAAGAATAAAAAATA
AATCAACATATTAAATTGTA

Downstream 100 bases:

>100_bases
AAATAAAGGTCTATGTACTAAATAACTTAGTTTTACAACGACTGACATTCATGATATGTCAAATGAGGTTGATGACTAAT
TATTGTACATAGACCTTTTT

Product: hypothetical protein

Products: NADP+; reduced thioredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MLFNSVANNSLCTLNKLSKIYHKSSSTVLAHQSLNCYNSLGLKEAETMQKVESIIIGGGPCGLSVAIEQKRKGIDTLIIE
KGNVVESIYNYPTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKKMNNKFTITTTK
DVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVVIIGGKNSAIDAALELEKAGANVTVLYRGGDYSP
SIKPWILPNFTALVNHEKIDMEFNANVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAP
MYNKETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQSMLAKKQTPLES

Sequences:

>Translated_375_residues
MLFNSVANNSLCTLNKLSKIYHKSSSTVLAHQSLNCYNSLGLKEAETMQKVESIIIGGGPCGLSVAIEQKRKGIDTLIIE
KGNVVESIYNYPTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKKMNNKFTITTTK
DVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVVIIGGKNSAIDAALELEKAGANVTVLYRGGDYSP
SIKPWILPNFTALVNHEKIDMEFNANVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAP
MYNKETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQSMLAKKQTPLES
>Mature_375_residues
MLFNSVANNSLCTLNKLSKIYHKSSSTVLAHQSLNCYNSLGLKEAETMQKVESIIIGGGPCGLSVAIEQKRKGIDTLIIE
KGNVVESIYNYPTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKKMNNKFTITTTK
DVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVVIIGGKNSAIDAALELEKAGANVTVLYRGGDYSP
SIKPWILPNFTALVNHEKIDMEFNANVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAP
MYNKETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQSMLAKKQTPLES

Specific function: Unknown

COG id: COG0492

COG function: function code O; Thioredoxin reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 808 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.8.1.9 [C]

Molecular weight: Translated: 41921; Mature: 41921

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFNSVANNSLCTLNKLSKIYHKSSSTVLAHQSLNCYNSLGLKEAETMQKVESIIIGGGP
CCCCCCCCCCEEEHHHHHHHHHCCCCEEEEECCCCHHHHCCCCHHHHHHHHHHEEECCCC
CGLSVAIEQKRKGIDTLIIEKGNVVESIYNYPTHQTFFSSSDKLSIGDVPFIVEESKPRR
CCEEEEEHHHHCCCCEEEEECCCHHHHHHCCCCCCEEECCCCCEEECCCCEEEECCCCCC
NQALVYYREVVKHHQLKVNAFEEVLTVKKMNNKFTITTTKDVYECRFLTIATGYYGQHNT
CCHHHHHHHHHHHHCEEEHHHHHHHHHHHCCCEEEEEECCCCEEEEEEEEEECCCCCCCE
LEVEGADLPKVFHYFKEAHPYFDQDVVIIGGKNSAIDAALELEKAGANVTVLYRGGDYSP
EEECCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCHHEEEHHHCCCCEEEEEECCCCCC
SIKPWILPNFTALVNHEKIDMEFNANVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPD
CCCCEECCCCCEEECCEEEEEEECCCEEEEECCEEEEEECCCCEEEECCEEEEEEECCCC
YEFLKSVGIQINTNEFGTAPMYNKETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGI
HHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEECCCCCCHH
IAQSMLAKKQTPLES
HHHHHHHHCCCCCCC
>Mature Secondary Structure
MLFNSVANNSLCTLNKLSKIYHKSSSTVLAHQSLNCYNSLGLKEAETMQKVESIIIGGGP
CCCCCCCCCCEEEHHHHHHHHHCCCCEEEEECCCCHHHHCCCCHHHHHHHHHHEEECCCC
CGLSVAIEQKRKGIDTLIIEKGNVVESIYNYPTHQTFFSSSDKLSIGDVPFIVEESKPRR
CCEEEEEHHHHCCCCEEEEECCCHHHHHHCCCCCCEEECCCCCEEECCCCEEEECCCCCC
NQALVYYREVVKHHQLKVNAFEEVLTVKKMNNKFTITTTKDVYECRFLTIATGYYGQHNT
CCHHHHHHHHHHHHCEEEHHHHHHHHHHHCCCEEEEEECCCCEEEEEEEEEECCCCCCCE
LEVEGADLPKVFHYFKEAHPYFDQDVVIIGGKNSAIDAALELEKAGANVTVLYRGGDYSP
EEECCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCHHEEEHHHCCCCEEEEEECCCCCC
SIKPWILPNFTALVNHEKIDMEFNANVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPD
CCCCEECCCCCEEECCEEEEEEECCCEEEEECCEEEEEECCCCEEEECCEEEEEEECCCC
YEFLKSVGIQINTNEFGTAPMYNKETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGI
HHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEECCCCCCHH
IAQSMLAKKQTPLES
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: NA

Kcat value (1/min): 1365 [C]

Specific activity: NA

Km value (mM): 0.125 {thioredoxin} 0.008 {NADPH}} 0.002 {thioredoxin}} 0.0017 {thioredoxin}} [C]

Substrates: NADPH; oxidized thioredoxin [C]

Specific reaction: NADPH + oxidized thioredoxin = NADP+ + reduced thioredoxin [C]

General reaction: Redox reaction [C]

Inhibitor: Ca2+; N-Ethyl maleimide; NADP+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377 [H]