BacMap

Definition	Staphylococcus aureus RF122, complete genome.
Accession	NC_007622
Length	2,742,531

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The map label for this gene is dkgA [H]

Identifier: 82750405

GI number: 82750405

Start: 718501

End: 719340

Strand: Direct

Name: dkgA [H]

Synonym: SAB0652

Alternate gene names: 82750405

Gene position: 718501-719340 (Clockwise)

Preceding gene: 82750404

Following gene: 82750406

Centisome position: 26.2

GC content: 32.14

Gene sequence:

>840_bases
ATGTTGAATGAGATACAAATATTAAATAATGGATACCCGATGCCTTCAGTTGGGTTAGGTGTTTATAAAATCTCTGACGA
AGATATGACTAAAGTTGTAAATGTTGCAATTGACGCAGGCTATAGAGCGTTTGATACAGCATACTTTTATGATAATGAGG
CTTCACTAGGGCGAGCATTAAAGGATAATGGCGTCGATAGAGAAGATTTGTTTATAACAACGAAGTTATGGAATGACTAT
CAAGGTTATGAGAAAACATTCGAATATTTCAACAAATCGATTGAAAATTTACAAACTGATTATCTCGATTTATTTCTAAT
ACATTGGCCTTGTGAAGCAGATGGTCTATTTTTAGAAACGTATAAAGCTATGGAAGAACTTTACGAGCAAGGTAAGGTAA
AAGCAATAGGTGTATGTAATTTTAATGTTCATCATCTAGAAAAATTAATGGCTCAATCAAGTATCAAACCAATGGTGAAT
CAAATTGAGGTACATCCATATTTTAACCAACAAGAATTACAAGAATTTTGTGATCGTCACGATATTAAAGTGACTGCATG
GATGCCTTTGATGAGAAATAGAGGACTACTAGACGACCCTGTCATTGTTAAAATTGCTGAAAAATATCATAAAACACCAG
CACAAGTTGTATTGCGTTGGCATTTAGCACACAATAGAATTATTATTCCAAAATCTCAGACACCTAAACGCATTCAAGAA
AATATAGATATTTTAGATTTTAATTTAGAATTAACAGAAGTAGCTGAAATTGATGCTTTAAATAGAAATGCAAGACAAGG
TAAAAATCCAGATGATGTGAAAATTGGGGATTTAAAATAA

Upstream 100 bases:

>100_bases
TGTTTTTTATTGAAAGCGATAATTCGTATTAATTGAGTTTGCTGAAAAATTTAGGGTAATGTAAAGATATAAAAGATACA
TAGATTGGAGAGATATAAAG

Downstream 100 bases:

>100_bases
CTGGATGTTAAATTTTACGTTTATGAATGCCTTTTAATGTGTACATTAAAATAAATGAGTTGGTTTTTACTATTTGATAA
AACAACACTCAGGTACATTC

Product: oxidoreductase

Products: NA

Alternate protein names: 2,5-DKG reductase A; 2,5-DKGR A; 25DKGR-A; AKR5C [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNVAIDAGYRAFDTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDY
QGYEKTFEYFNKSIENLQTDYLDLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN
QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDDPVIVKIAEKYHKTPAQVVLRWHLAHNRIIIPKSQTPKRIQE
NIDILDFNLELTEVAEIDALNRNARQGKNPDDVKIGDLK

Sequences:

>Translated_279_residues
MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNVAIDAGYRAFDTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDY
QGYEKTFEYFNKSIENLQTDYLDLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN
QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDDPVIVKIAEKYHKTPAQVVLRWHLAHNRIIIPKSQTPKRIQE
NIDILDFNLELTEVAEIDALNRNARQGKNPDDVKIGDLK
>Mature_279_residues
MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNVAIDAGYRAFDTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDY
QGYEKTFEYFNKSIENLQTDYLDLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN
QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDDPVIVKIAEKYHKTPAQVVLRWHLAHNRIIIPKSQTPKRIQE
NIDILDFNLELTEVAEIDALNRNARQGKNPDDVKIGDLK

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI223468663, Length=291, Percent_Identity=40.893470790378, Blast_Score=213, Evalue=1e-55,
Organism=Homo sapiens, GI24497577, Length=297, Percent_Identity=37.7104377104377, Blast_Score=210, Evalue=1e-54,
Organism=Homo sapiens, GI5174391, Length=297, Percent_Identity=37.7104377104377, Blast_Score=210, Evalue=1e-54,
Organism=Homo sapiens, GI93277124, Length=303, Percent_Identity=39.9339933993399, Blast_Score=208, Evalue=4e-54,
Organism=Homo sapiens, GI291291012, Length=286, Percent_Identity=39.5104895104895, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI310109922, Length=277, Percent_Identity=37.9061371841155, Blast_Score=197, Evalue=6e-51,
Organism=Homo sapiens, GI310109920, Length=295, Percent_Identity=36.271186440678, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI5453543, Length=295, Percent_Identity=35.9322033898305, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI45446745, Length=295, Percent_Identity=36.271186440678, Blast_Score=191, Evalue=6e-49,
Organism=Homo sapiens, GI4503285, Length=295, Percent_Identity=36.271186440678, Blast_Score=191, Evalue=6e-49,
Organism=Homo sapiens, GI4502049, Length=292, Percent_Identity=40.4109589041096, Blast_Score=188, Evalue=6e-48,
Organism=Homo sapiens, GI24497583, Length=293, Percent_Identity=34.4709897610922, Blast_Score=181, Evalue=6e-46,
Organism=Homo sapiens, GI5174695, Length=296, Percent_Identity=36.1486486486487, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI24497585, Length=295, Percent_Identity=34.5762711864407, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI300116273, Length=275, Percent_Identity=34.9090909090909, Blast_Score=161, Evalue=6e-40,
Organism=Homo sapiens, GI300116271, Length=279, Percent_Identity=34.4086021505376, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI310109924, Length=154, Percent_Identity=38.3116883116883, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI310109926, Length=112, Percent_Identity=39.2857142857143, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI207028673, Length=112, Percent_Identity=39.2857142857143, Blast_Score=88, Evalue=9e-18,
Organism=Escherichia coli, GI87082198, Length=265, Percent_Identity=45.2830188679245, Blast_Score=261, Evalue=4e-71,
Organism=Escherichia coli, GI1786400, Length=254, Percent_Identity=36.2204724409449, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1788081, Length=262, Percent_Identity=31.6793893129771, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI87081735, Length=294, Percent_Identity=22.7891156462585, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17537077, Length=290, Percent_Identity=37.9310344827586, Blast_Score=198, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17537075, Length=290, Percent_Identity=38.2758620689655, Blast_Score=197, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17561298, Length=273, Percent_Identity=37.3626373626374, Blast_Score=195, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI71998625, Length=266, Percent_Identity=36.4661654135338, Blast_Score=186, Evalue=9e-48,
Organism=Caenorhabditis elegans, GI17564128, Length=295, Percent_Identity=34.5762711864407, Blast_Score=178, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17537079, Length=296, Percent_Identity=32.4324324324324, Blast_Score=173, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI17538386, Length=270, Percent_Identity=35.5555555555556, Blast_Score=170, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI17566692, Length=294, Percent_Identity=34.3537414965986, Blast_Score=168, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17561300, Length=269, Percent_Identity=36.8029739776952, Blast_Score=167, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17562292, Length=274, Percent_Identity=33.9416058394161, Blast_Score=164, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17550248, Length=291, Percent_Identity=32.9896907216495, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17552492, Length=276, Percent_Identity=31.1594202898551, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17550246, Length=75, Percent_Identity=45.3333333333333, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6324694, Length=277, Percent_Identity=36.8231046931408, Blast_Score=186, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6319625, Length=273, Percent_Identity=37.3626373626374, Blast_Score=177, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6322556, Length=267, Percent_Identity=34.8314606741573, Blast_Score=176, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6320576, Length=269, Percent_Identity=35.6877323420074, Blast_Score=173, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6321896, Length=311, Percent_Identity=33.4405144694534, Blast_Score=163, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6320079, Length=279, Percent_Identity=32.258064516129, Blast_Score=132, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24663317, Length=288, Percent_Identity=40.625, Blast_Score=212, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24657054, Length=287, Percent_Identity=39.3728222996516, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI21356425, Length=287, Percent_Identity=39.0243902439024, Blast_Score=195, Evalue=3e-50,
Organism=Drosophila melanogaster, GI24644950, Length=297, Percent_Identity=38.3838383838384, Blast_Score=192, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24662789, Length=293, Percent_Identity=37.8839590443686, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI221378297, Length=317, Percent_Identity=35.9621451104101, Blast_Score=184, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24662781, Length=289, Percent_Identity=39.4463667820069, Blast_Score=177, Evalue=8e-45,
Organism=Drosophila melanogaster, GI45553081, Length=287, Percent_Identity=34.4947735191638, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24662785, Length=287, Percent_Identity=39.0243902439024, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI20129731, Length=283, Percent_Identity=34.2756183745583, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI281366140, Length=255, Percent_Identity=33.7254901960784, Blast_Score=150, Evalue=8e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 32388; Mature: 32388

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNVAIDAGYRAFDTAYFYDNEASLGRAL
CCCCEEEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCHHEEEEEECCCHHHHHHH
KDNGVDREDLFITTKLWNDYQGYEKTFEYFNKSIENLQTDYLDLFLIHWPCEADGLFLET
HHCCCCHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCHHHH
YKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVNQIEVHPYFNQQELQEFCDRH
HHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHC
DIKVTAWMPLMRNRGLLDDPVIVKIAEKYHKTPAQVVLRWHLAHNRIIIPKSQTPKRIQE
CCEEEEECHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHEEECCCEEEECCCCCHHHHHC
NIDILDFNLELTEVAEIDALNRNARQGKNPDDVKIGDLK
CCCEEEECCCHHHHHHHHHHCCHHHCCCCCCCCEECCCC
>Mature Secondary Structure
MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNVAIDAGYRAFDTAYFYDNEASLGRAL
CCCCEEEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCHHEEEEEECCCHHHHHHH
KDNGVDREDLFITTKLWNDYQGYEKTFEYFNKSIENLQTDYLDLFLIHWPCEADGLFLET
HHCCCCHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCHHHH
YKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVNQIEVHPYFNQQELQEFCDRH
HHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHC
DIKVTAWMPLMRNRGLLDDPVIVKIAEKYHKTPAQVVLRWHLAHNRIIIPKSQTPKRIQE
CCEEEEECHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHEEECCCEEEECCCCCHHHHHC
NIDILDFNLELTEVAEIDALNRNARQGKNPDDVKIGDLK
CCCEEEECCCHHHHHHHHHHCCHHHCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]