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The map label for this gene is pit [H]

Identifier: 82750366

GI number: 82750366

Start: 679417

End: 680424

Strand: Direct

Name: pit [H]

Synonym: SAB0613

Alternate gene names: 82750366

Gene position: 679417-680424 (Clockwise)

Preceding gene: 82750365

Following gene: 82750368

Centisome position: 24.77

GC content: 36.9

Gene sequence:

>1008_bases
ATGTCATATATAATCATCGTCACTATAGCTGTAGTTATTTTCTCGCTGATATTTGACTTTATCAATGGATTCCATGATAC
AGCCAATGCAGTAGCTACTGCTGTATCTACTAGAGCGTTAACGCCTAAAACGGCAATTTTAATGGCAGCAGTGATGAACT
TTATAGGTGCTTTAACATTTACGGGTGTTGCAGGCACCATTACTAAAGACATTGTCGATCCATTTAAATTGGAAAATGGA
TTAGTTGTTGTGTTAGCTGCAATACTTGCGGCTATTATTTGGAATTTAGCTACTTGGTTTTACGGAATTCCAAGTTCGTC
TTCACATGCACTTATAGGTTCAATTGCGGGTGCAGCAATCGCATCTGAAGGCTCATTTGGAGTGTTACATTACCAAGGTT
TCACAAAAATTATTATTGTATTAATCGTTTCGCCGATTATCGCATTTTGTGTTGGTTTCTTGATGTATTCAATTTTTAAA
GTTATCTTTAAAAACGCAAATTTAACAAGAGCGAATCGTAATTTTAGATTTTTCCAAATTTTCACAGCAGCGTTACAATC
ATTCTCTCACGGTACGAATGATGCGCAAAAATCAATGGGTATTATTACGTTGGCATTGATTGTTGCTAATGTACAGAATG
ATGGCAGCGTTGAACCACAGTTATGGGTAAAATTTGCCTGTGCGACAGCAATGGGGCTTGGTACTGCAATTGGTGGCTGG
AAAATTATCAAAACTGTAGGTGGTAATATTATGAAAATACGTCCTGCAAATGGTGCAGCGGCCGATTTATCATCTGCATT
AACAATTTTTGTTGCGTCATCTCTGCATTTCCCATTATCAACTACACACGTTGTGTCATCATCAATTTTAGGTGTTGGTG
CTTCTAACAGAGCTAAAGGTGTTAAATGGAGCACTGCTCAACGTATGATTATTACATGGGTGATTACATTACCGATATCT
GCGTTTTTAGCAGCGTTTTTATTCTATATACTTAGCTTATTTTTCTAA

Upstream 100 bases:

>100_bases
TATTTATGAAAGCATGGAAGAAATTGCTGATAAATGTCAAATCGTAGCAAATAATTTTGAAACTATTATTATGAAAAATA
GCTAAGGGGAGTATATATTT

Downstream 100 bases:

>100_bases
TTGAAAATTAAGCTATACTGAACTTCAGTATCACAAACATATGGTGGTATTGAAGTTCATTTTTTTATGTAAATAGATAA
CATATTTTATAGAGTTTACA

Product: low-affinity inorganic phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MSYIIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKTAILMAAVMNFIGALTFTGVAGTITKDIVDPFKLENG
LVVVLAAILAAIIWNLATWFYGIPSSSSHALIGSIAGAAIASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFK
VIFKNANLTRANRNFRFFQIFTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVEPQLWVKFACATAMGLGTAIGGW
KIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLHFPLSTTHVVSSSILGVGASNRAKGVKWSTAQRMIITWVITLPIS
AFLAAFLFYILSLFF

Sequences:

>Translated_335_residues
MSYIIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKTAILMAAVMNFIGALTFTGVAGTITKDIVDPFKLENG
LVVVLAAILAAIIWNLATWFYGIPSSSSHALIGSIAGAAIASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFK
VIFKNANLTRANRNFRFFQIFTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVEPQLWVKFACATAMGLGTAIGGW
KIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLHFPLSTTHVVSSSILGVGASNRAKGVKWSTAQRMIITWVITLPIS
AFLAAFLFYILSLFF
>Mature_334_residues
SYIIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKTAILMAAVMNFIGALTFTGVAGTITKDIVDPFKLENGL
VVVLAAILAAIIWNLATWFYGIPSSSSHALIGSIAGAAIASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFKV
IFKNANLTRANRNFRFFQIFTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVEPQLWVKFACATAMGLGTAIGGWK
IIKTVGGNIMKIRPANGAAADLSSALTIFVASSLHFPLSTTHVVSSSILGVGASNRAKGVKWSTAQRMIITWVITLPISA
FLAAFLFYILSLFF

Specific function: Low-affinity inorganic phosphate transport [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=165, Percent_Identity=32.1212121212121, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI5803173, Length=158, Percent_Identity=29.746835443038, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1789360, Length=217, Percent_Identity=36.8663594470046, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1789907, Length=217, Percent_Identity=38.7096774193548, Blast_Score=120, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI25146401, Length=174, Percent_Identity=36.7816091954023, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17539280, Length=166, Percent_Identity=31.3253012048193, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71981576, Length=175, Percent_Identity=35.4285714285714, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI32566716, Length=177, Percent_Identity=28.2485875706215, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17536725, Length=169, Percent_Identity=28.4023668639053, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6319773, Length=160, Percent_Identity=29.375, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI21356511, Length=187, Percent_Identity=29.9465240641711, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 35643; Mature: 35512

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYIIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKTAILMAAVMNFIGALTF
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
TGVAGTITKDIVDPFKLENGLVVVLAAILAAIIWNLATWFYGIPSSSSHALIGSIAGAAI
HCCHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
ASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFKVIFKNANLTRANRNFRFFQI
CCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHH
FTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVEPQLWVKFACATAMGLGTAIGGW
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCH
KIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLHFPLSTTHVVSSSILGVGASNRAKG
HHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCC
VKWSTAQRMIITWVITLPISAFLAAFLFYILSLFF
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SYIIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKTAILMAAVMNFIGALTF
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
TGVAGTITKDIVDPFKLENGLVVVLAAILAAIIWNLATWFYGIPSSSSHALIGSIAGAAI
HCCHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
ASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFKVIFKNANLTRANRNFRFFQI
CCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHH
FTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVEPQLWVKFACATAMGLGTAIGGW
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCH
KIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLHFPLSTTHVVSSSILGVGASNRAKG
HHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCC
VKWSTAQRMIITWVITLPISAFLAAFLFYILSLFF
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]