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The map label for this gene is gltX

Identifier: 82750235

GI number: 82750235

Start: 537195

End: 538649

Strand: Direct

Name: gltX

Synonym: SAB0478

Alternate gene names: 82750235

Gene position: 537195-538649 (Clockwise)

Preceding gene: 82750234

Following gene: 82750236

Centisome position: 19.59

GC content: 34.23

Gene sequence:

>1455_bases
ATGAGCGATCGTATAAGAGTAAGATATGCACCAAGTCCAACTGGTTATCTTCATATTGGTAATGCAAGAACAGCATTATT
CAATTACTTGTATGCTAAACATTACAACGGAGATTTTGTGATTCGAATTGAAGATACTGATAAAAAACGTAATTTAGAAG
ATGGAGAAACATCACAATTTGATAATCTTAAATGGTTAGGATTAGATTGGGATGAGTCTGTAGATAAAGACAATGGCTAC
GGACCATATCGTCAATCTGAACGTCAACATATATACCAACCATTAATAGATCAGTTACTAGCAGAAGATAAAGCATATAA
ATGCTATATGACAGAAGAAGAATTAGAAGTTGAACGTGAAGCGCAAATCGCTCGTGGTGAAATGCCTCGCTATGGTGGTC
AACATGCGCATTTGACTGAAGAACAACGTCAACAATTTGAAGCAGAAGGACGCCAACCATCAATTCGTTTCCGAGTACCT
CAAAACCAAACGTATTCATTTGATGATATGGTAAAAGGGAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTAT
CGTTAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGGAAATTTCAGATGTTATTC
GTGGTGATGATCATATTTCAAACACGCCTAAACAAATTATGATTTATGAAGCATTTGGCTGGGAGCCACCTCGTTTTGGT
CATATGTCATTAATTGTTAATGAAGAACGTAAAAAGTTAAGTAAACGTGATGGGCAAATTTTACAATTTATTGAGCAATA
TCGTGACTTAGGTTATTTACCTGAAGCGTTATTTAATTTTATTGCGTTATTAGGTTGGTCTCCTGAAGGTGAAGAAGAAA
TCTTTTCTAAAGAAGAATTTATCAAAATCTTTGATGAAAAGCGTTTGTCAAAATCACCAGCATTTTTCGATAAGCAAAAA
TTAGCATGGGTTAATAACCAATATATGAAACAAAAAGATACTGAAACAGTATTCCAATTAGCATTACCTCATTTAATTAA
AGCGAATTTGATTCCTGAGGTGCCGTCAGAAGAGGATTTATCTTGGGGACGCAAATTAATTGCTCTTTATCAAAAAGAAA
TGAGCTATGCCGGTGAAATTGTTCCTTTATCAGAAATGTTCTTTAAAGAAATGCCAGCTCTTGGTGAAGAAGAACAACAA
GTGATCAATGGAGAGCAAGTACCAGAGTTAATGACGCACTTATTCAGTAAATTAGAAGCACTTGAACCATTTGAAGTGGC
TGAAATTAAAAAGACAATTAAAGAAGTTCAAAAAGAAACAGGAATAAAAGGCAAGCAATTATTTATGCCTATTCGTGTTG
CTGTAACAGGCCAAATGCATGGTCCTGAATTACCAAATACAATTGAAGTACTTGGAAAAGAAAAAGTGCTAAACCGTTTA
AAACAATATAAGTAA

Upstream 100 bases:

>100_bases
TAAGACGATTTACACATTTGTTATGACGGTCGAAGATAGCAAATGAACAATTTATATGAACTAACTTGAAAATCTGATAT
TTAATTTAGGAGTGAAAAAA

Downstream 100 bases:

>100_bases
TGAAATAGACAAGATCAAGGTAGTATATACTTGAATTTTATAACGTAACCACATATGATAAAGTTGTATTAACAAATTAC
AACAATATGAATAGAGTATT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIEDTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGY
GPYRQSERQHIYQPLIDQLLAEDKAYKCYMTEEELEVEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMEISDVIRGDDHISNTPKQIMIYEAFGWEPPRFG
HMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEALFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQK
LAWVNNQYMKQKDTETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMPALGEEEQQ
VINGEQVPELMTHLFSKLEALEPFEVAEIKKTIKEVQKETGIKGKQLFMPIRVAVTGQMHGPELPNTIEVLGKEKVLNRL
KQYK

Sequences:

>Translated_484_residues
MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIEDTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGY
GPYRQSERQHIYQPLIDQLLAEDKAYKCYMTEEELEVEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMEISDVIRGDDHISNTPKQIMIYEAFGWEPPRFG
HMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEALFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQK
LAWVNNQYMKQKDTETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMPALGEEEQQ
VINGEQVPELMTHLFSKLEALEPFEVAEIKKTIKEVQKETGIKGKQLFMPIRVAVTGQMHGPELPNTIEVLGKEKVLNRL
KQYK
>Mature_483_residues
SDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIEDTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYG
PYRQSERQHIYQPLIDQLLAEDKAYKCYMTEEELEVEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVPQ
NQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMEISDVIRGDDHISNTPKQIMIYEAFGWEPPRFGH
MSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEALFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKL
AWVNNQYMKQKDTETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMPALGEEEQQV
INGEQVPELMTHLFSKLEALEPFEVAEIKKTIKEVQKETGIKGKQLFMPIRVAVTGQMHGPELPNTIEVLGKEKVLNRLK
QYK

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=490, Percent_Identity=31.0204081632653, Blast_Score=251, Evalue=8e-67,
Organism=Escherichia coli, GI1788743, Length=480, Percent_Identity=37.7083333333333, Blast_Score=328, Evalue=7e-91,
Organism=Escherichia coli, GI226510925, Length=251, Percent_Identity=29.8804780876494, Blast_Score=121, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71993394, Length=492, Percent_Identity=32.520325203252, Blast_Score=241, Evalue=5e-64,
Organism=Saccharomyces cerevisiae, GI6324540, Length=501, Percent_Identity=32.7345309381238, Blast_Score=261, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6321192, Length=213, Percent_Identity=25.8215962441315, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6324742, Length=146, Percent_Identity=31.5068493150685, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24665321, Length=498, Percent_Identity=32.9317269076305, Blast_Score=275, Evalue=4e-74,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 56347; Mature: 56215

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIEDTDKKRNLEDGETSQF
CCCCEEEEECCCCCCEEEECCHHHHHHHHHHHEECCCEEEEEEECCCCCCCCCCCCCCCC
DNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAEDKAYKCYMTEEELEVERE
CCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHH
AQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVPQNQTYSFDDMVKGNISFDSN
HHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHCCCCCCCCC
GIGDWVIVKKDGIPTYNFAVAIDDHYMEISDVIRGDDHISNTPKQIMIYEAFGWEPPRFG
CCCCEEEEECCCCCCEEEEEEECCCHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCC
HMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEALFNFIALLGWSPEGEEEIFSKEEF
CEEEEECHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
IKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDTETVFQLALPHLIKANLIPEVPSEEDL
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
SWGRKLIALYQKEMSYAGEIVPLSEMFFKEMPALGEEEQQVINGEQVPELMTHLFSKLEA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHCHHHHHHHHHHHHHHHHH
LEPFEVAEIKKTIKEVQKETGIKGKQLFMPIRVAVTGQMHGPELPNTIEVLGKEKVLNRL
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
KQYK
HCCC
>Mature Secondary Structure 
SDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIEDTDKKRNLEDGETSQF
CCCEEEEECCCCCCEEEECCHHHHHHHHHHHEECCCEEEEEEECCCCCCCCCCCCCCCC
DNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAEDKAYKCYMTEEELEVERE
CCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHH
AQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVPQNQTYSFDDMVKGNISFDSN
HHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHCCCCCCCCC
GIGDWVIVKKDGIPTYNFAVAIDDHYMEISDVIRGDDHISNTPKQIMIYEAFGWEPPRFG
CCCCEEEEECCCCCCEEEEEEECCCHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCC
HMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEALFNFIALLGWSPEGEEEIFSKEEF
CEEEEECHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
IKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDTETVFQLALPHLIKANLIPEVPSEEDL
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
SWGRKLIALYQKEMSYAGEIVPLSEMFFKEMPALGEEEQQVINGEQVPELMTHLFSKLEA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHCHHHHHHHHHHHHHHHHH
LEPFEVAEIKKTIKEVQKETGIKGKQLFMPIRVAVTGQMHGPELPNTIEVLGKEKVLNRL
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
KQYK
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA