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The map label for this gene is tilS [H]

Identifier: 82750216

GI number: 82750216

Start: 505447

End: 506742

Strand: Direct

Name: tilS [H]

Synonym: SAB0458

Alternate gene names: 82750216

Gene position: 505447-506742 (Clockwise)

Preceding gene: 82750215

Following gene: 82750217

Centisome position: 18.43

GC content: 30.79

Gene sequence:

>1296_bases
ATGCAGTTAAATAGTAATGGTTGGCATGTTGATGACCATATTGTTGTCGCTGTTTCTACAGGTATTGATAGTATGTGTTT
ATTGTATCAATTACTAAATGATTATAAAGATAGTTATAGAAAACTAACATGTTTACATGTCAATCATGGCGTTAGGTCAG
CTTCAATCGAGGAAGCCAGATTTTTAGAAGCATACTGCGAAGGTCATCACATCGATTTACATATCAAAAAGTTGGATTTG
TCGCATAGTCTCAATCGAAATAACAGCATTCAGAATGAAGCTCGAATTAAACGTTACGAATGGTTTGATGAAATGATGAA
TGTATTAGAAGCGGATGTATTGCTAACGGCGCATCATTTGGACGATCAATTAGAAACTATTATGTATCGCATTTTTAATG
GGAAATCAACGCGTAATAAACTAGGATTTGATGAGTTATCGAAGCGAAAAGGTTATCAGATTTATAGACCACTTTTAGCT
GTCTCTAAAAAAGAAATAAAACAATTCCAAGAGAGATATCATATACCATATTTTGAAGATGAATCTAATAAAGATAACAA
ATATGTTAGAAATGATATTCGTAATAGAATTATTCCAGCTATTGATGAAAATAATCAACTTAAAGTATCGCATTTATTAA
AATTAAAACAATGGCATGATGAACAATATGATATTTTGCAATATTCAGCTAAACAATTTATTCAAGAATTTGTGAAGTTT
GATGAACAGTCAAAATATTTAGAGGTTTCTAGACAAGCTTTTAATAACTTGCCAAACTCATTAAAGGTGGTTGTGTTGGA
TTGCCTATTATCAAAGTATTATGAGTTGTTTAATATTAGTGCTAAAACATACGAAGAGTGGTTTAAACAATTTAGTAGTA
AGAAAGCACAATTCAGTATTAATCTCACGGATAAATGGATAATTCAAATCGCATATGGTAAATTAATAATAATGGCTAAA
AATAATGGCGATACATATTTTAGAGTTCAAACTATTGAAAAGCCAGGTAATTATATTTTTAACAAATATCGATTAGAGAT
ACATTCTAATTTACCAAAATGTTTATTTCCGCTTACAGTGAGAACACGACAAAGTGGCGATACATTTAAACTGAATGGGC
ACGATGGTTATAAGAAAGTGAATCGCCTGTTTATAGATTGTAAAGTGCAACAGTGGGTTCGGGATCAAATGCCAATCGTA
TTGGATAAACAACAGCGCATTATTGCGGTAGGAGATTTATATCAACAACAAACAATAAAACAATGGATTATAATTAGTAA
AAATGGAGATGAATAG

Upstream 100 bases:

>100_bases
ATCTAAGACTGTTTCTTAAATAAGGAGCAGTCTCTTTTATTTGTAATGATATAACTAAGACTTATACCATTTTTGAAAAT
TGTAAAAGTGAGGTGATGTT

Downstream 100 bases:

>100_bases
CGTTATGCATAATGATTTGAAAGAAGTATTGTTAACTGAAGAAGATATTCAAAATATCTGTAAGGAATTGGGAGCACAAT
TAACAAAGGATTATCAAGGT

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTCLHVNHGVRSASIEEARFLEAYCEGHHIDLHIKKLDL
SHSLNRNNSIQNEARIKRYEWFDEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLA
VSKKEIKQFQERYHIPYFEDESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQWHDEQYDILQYSAKQFIQEFVKF
DEQSKYLEVSRQAFNNLPNSLKVVVLDCLLSKYYELFNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAK
NNGDTYFRVQTIEKPGNYIFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGHDGYKKVNRLFIDCKVQQWVRDQMPIV
LDKQQRIIAVGDLYQQQTIKQWIIISKNGDE

Sequences:

>Translated_431_residues
MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTCLHVNHGVRSASIEEARFLEAYCEGHHIDLHIKKLDL
SHSLNRNNSIQNEARIKRYEWFDEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLA
VSKKEIKQFQERYHIPYFEDESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQWHDEQYDILQYSAKQFIQEFVKF
DEQSKYLEVSRQAFNNLPNSLKVVVLDCLLSKYYELFNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAK
NNGDTYFRVQTIEKPGNYIFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGHDGYKKVNRLFIDCKVQQWVRDQMPIV
LDKQQRIIAVGDLYQQQTIKQWIIISKNGDE
>Mature_431_residues
MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTCLHVNHGVRSASIEEARFLEAYCEGHHIDLHIKKLDL
SHSLNRNNSIQNEARIKRYEWFDEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLA
VSKKEIKQFQERYHIPYFEDESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQWHDEQYDILQYSAKQFIQEFVKF
DEQSKYLEVSRQAFNNLPNSLKVVVLDCLLSKYYELFNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAK
NNGDTYFRVQTIEKPGNYIFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGHDGYKKVNRLFIDCKVQQWVRDQMPIV
LDKQQRIIAVGDLYQQQTIKQWIIISKNGDE

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=192, Percent_Identity=32.2916666666667, Blast_Score=111, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 51173; Mature: 51173

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTCLHVNHGVRSASIEEAR
CCCCCCCCEECCEEEEEEECCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHH
FLEAYCEGHHIDLHIKKLDLSHSLNRNNSIQNEARIKRYEWFDEMMNVLEADVLLTAHHL
HHHHHHCCCEEEEEEEEECCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLAVSKKEIKQFQERYHIPYFED
HHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQWHDEQYDILQYSAKQFIQEFVKF
CCCCCHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DEQSKYLEVSRQAFNNLPNSLKVVVLDCLLSKYYELFNISAKTYEEWFKQFSSKKAQFSI
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEE
NLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIEKPGNYIFNKYRLEIHSNLPKCLFPLTV
ECCCEEEEEEEECEEEEEEECCCCEEEEEEEECCCCCEEEEEEEEEECCCCCHHEECEEE
RTRQSGDTFKLNGHDGYKKVNRLFIDCKVQQWVRDQMPIVLDKQQRIIAVGDLYQQQTIK
EECCCCCEEEECCCCHHHHHHHEEEEEEHHHHHHCCCCEEECCCCCEEEEHHHHHHHHHH
QWIIISKNGDE
EEEEEECCCCC
>Mature Secondary Structure
MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTCLHVNHGVRSASIEEAR
CCCCCCCCEECCEEEEEEECCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHH
FLEAYCEGHHIDLHIKKLDLSHSLNRNNSIQNEARIKRYEWFDEMMNVLEADVLLTAHHL
HHHHHHCCCEEEEEEEEECCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLAVSKKEIKQFQERYHIPYFED
HHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQWHDEQYDILQYSAKQFIQEFVKF
CCCCCHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DEQSKYLEVSRQAFNNLPNSLKVVVLDCLLSKYYELFNISAKTYEEWFKQFSSKKAQFSI
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEE
NLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIEKPGNYIFNKYRLEIHSNLPKCLFPLTV
ECCCEEEEEEEECEEEEEEECCCCEEEEEEEECCCCCEEEEEEEEEECCCCCHHEECEEE
RTRQSGDTFKLNGHDGYKKVNRLFIDCKVQQWVRDQMPIVLDKQQRIIAVGDLYQQQTIK
EECCCCCEEEECCCCHHHHHHHEEEEEEHHHHHHCCCCEEECCCCCEEEEHHHHHHHHHH
QWIIISKNGDE
EEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA