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The map label for this gene is phoB [H]

Identifier: 82703349

GI number: 82703349

Start: 2534765

End: 2535469

Strand: Direct

Name: phoB [H]

Synonym: Nmul_A2231

Alternate gene names: 82703349

Gene position: 2534765-2535469 (Clockwise)

Preceding gene: 82703348

Following gene: 82703350

Centisome position: 79.6

GC content: 58.3

Gene sequence:

>705_bases
ATGGCAGCAACGATACTGGTGGTGGAAGATGAACCCGCGATCCAGGAGCTGATCTCATATAGTCTTCGGCAGGCCAAGCA
TACGGTTTCCTCGACAAAAAACGCAGAGGAAGCCATGAAAATCATCAATCATACGCTACCCGACCTGGTGCTCCTGGATT
GGATGCTGCCCGGGATGAGTGGCGCCGATTTTGCCCGCATGCTGCGGCGCGCTGCCCGCACAAAGGATATTCCCATTATT
ATGCTCACCGCCCGTGCGGACGAGAGCGACAAGGTGACCGGCCTGGAAAGCGGCGCCGACGATTACATTACCAAGCCTTT
TTCGCCCCGGGAACTGCTGGCGAGAATCAAGGCCGTGCTACGGCGGCGCGCTCCCGAAGCCGCCGACGATGTTGTGGAGA
TCGGTGGACTGCGCCTGGATTCGGCCACCCATCGCGTCACTGCGAATGAGATTGAAGTCGTGCTCGGGCCCACCGAATTC
CGCTTGCTGCACTATCTCATGACTCATTCCGAACGGGTACATAGCCGTTCCCAATTGCTCGATCAGGTATGGGGCGATCA
TGTATTTGTCGAGGACCGTACCGTGGATGTGCATATCCGCCGCCTGCGCAAGGCCCTCGAAAACGTCGACAAGGATGGAC
TGGTGCAGACAGTGCGGGGCTCCGGCTATCGCCTTTCGGCAGGTCAAGCAGTGAACGCGGGATGA

Upstream 100 bases:

>100_bases
GCTTGCAAATGTCACAAAATCGCAATACTGTTTACATATCATCGAAATATAAGAAAAAATCAAAAACGGAGAAGATTCTG
ACGACAGAAGGAATGATCTA

Downstream 100 bases:

>100_bases
AAGCGGGAATGATCTGTTGTAATCGTTCCAACTGACGTTCGTGTCCGGTTTCTGGGGGCATTTTGCCAATCTCGTACTGA
TTGCCGTTGTAAGCGGAGTA

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MAATILVVEDEPAIQELISYSLRQAKHTVSSTKNAEEAMKIINHTLPDLVLLDWMLPGMSGADFARMLRRAARTKDIPII
MLTARADESDKVTGLESGADDYITKPFSPRELLARIKAVLRRRAPEAADDVVEIGGLRLDSATHRVTANEIEVVLGPTEF
RLLHYLMTHSERVHSRSQLLDQVWGDHVFVEDRTVDVHIRRLRKALENVDKDGLVQTVRGSGYRLSAGQAVNAG

Sequences:

>Translated_234_residues
MAATILVVEDEPAIQELISYSLRQAKHTVSSTKNAEEAMKIINHTLPDLVLLDWMLPGMSGADFARMLRRAARTKDIPII
MLTARADESDKVTGLESGADDYITKPFSPRELLARIKAVLRRRAPEAADDVVEIGGLRLDSATHRVTANEIEVVLGPTEF
RLLHYLMTHSERVHSRSQLLDQVWGDHVFVEDRTVDVHIRRLRKALENVDKDGLVQTVRGSGYRLSAGQAVNAG
>Mature_233_residues
AATILVVEDEPAIQELISYSLRQAKHTVSSTKNAEEAMKIINHTLPDLVLLDWMLPGMSGADFARMLRRAARTKDIPIIM
LTARADESDKVTGLESGADDYITKPFSPRELLARIKAVLRRRAPEAADDVVEIGGLRLDSATHRVTANEIEVVLGPTEFR
LLHYLMTHSERVHSRSQLLDQVWGDHVFVEDRTVDVHIRRLRKALENVDKDGLVQTVRGSGYRLSAGQAVNAG

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=227, Percent_Identity=58.5903083700441, Blast_Score=280, Evalue=8e-77,
Organism=Escherichia coli, GI1788394, Length=226, Percent_Identity=41.5929203539823, Blast_Score=167, Evalue=4e-43,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=37.6681614349776, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1790860, Length=224, Percent_Identity=37.9464285714286, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1789809, Length=226, Percent_Identity=38.0530973451327, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1786784, Length=221, Percent_Identity=37.10407239819, Blast_Score=141, Evalue=3e-35,
Organism=Escherichia coli, GI1786911, Length=224, Percent_Identity=35.7142857142857, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1790863, Length=236, Percent_Identity=33.8983050847458, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1787375, Length=219, Percent_Identity=32.4200913242009, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1787229, Length=235, Percent_Identity=35.7446808510638, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI2367329, Length=178, Percent_Identity=39.8876404494382, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1789402, Length=222, Percent_Identity=32.4324324324324, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1790552, Length=230, Percent_Identity=33.4782608695652, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI145693140, Length=232, Percent_Identity=33.1896551724138, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1790299, Length=132, Percent_Identity=26.5151515151515, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26068; Mature: 25937

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATILVVEDEPAIQELISYSLRQAKHTVSSTKNAEEAMKIINHTLPDLVLLDWMLPGMS
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCCC
GADFARMLRRAARTKDIPIIMLTARADESDKVTGLESGADDYITKPFSPRELLARIKAVL
CHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHH
RRRAPEAADDVVEIGGLRLDSATHRVTANEIEVVLGPTEFRLLHYLMTHSERVHSRSQLL
HHCCCCHHHHHHHHCCEEECCCHHEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
DQVWGDHVFVEDRTVDVHIRRLRKALENVDKDGLVQTVRGSGYRLSAGQAVNAG
HHHCCCEEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEECCCCCCCCC
>Mature Secondary Structure 
AATILVVEDEPAIQELISYSLRQAKHTVSSTKNAEEAMKIINHTLPDLVLLDWMLPGMS
CEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCCC
GADFARMLRRAARTKDIPIIMLTARADESDKVTGLESGADDYITKPFSPRELLARIKAVL
CHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHH
RRRAPEAADDVVEIGGLRLDSATHRVTANEIEVVLGPTEFRLLHYLMTHSERVHSRSQLL
HHCCCCHHHHHHHHCCEEECCCHHEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
DQVWGDHVFVEDRTVDVHIRRLRKALENVDKDGLVQTVRGSGYRLSAGQAVNAG
HHHCCCEEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]