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The map label for this gene is pheA [H]

Identifier: 82703310

GI number: 82703310

Start: 2490323

End: 2491390

Strand: Direct

Name: pheA [H]

Synonym: Nmul_A2192

Alternate gene names: 82703310

Gene position: 2490323-2491390 (Clockwise)

Preceding gene: 82703309

Following gene: 82703311

Centisome position: 78.21

GC content: 56.93

Gene sequence:

>1068_bases
ATGACCGCTCAACTCAAACAGCTGCGCGACAAGATAGATGCAATCGACAGCGAACTCCTGAAGCTGGTGAGCGCGCGGGC
AGATCTCGCCCGCGAAATCGGAGAAATCAAGAATGGCACTGCGTATCGGCCCGAACGTGAAGCTCAGGTGCTGGCGCGGA
TGCGCGAACTGAACCCGGGGCCCTTGGAGAATGAACAGGTTGCGCGGTTATTTACGGAGATCATGTCTCTCTGTCGATCC
ATGGAAGAACCTCTGACAGTCGCATACCTGGGACCGAGGGGCACGTTTTCCGAAGAAGCTGCGCTCAAGCGCTTCGGTAG
CGTTGTGACTTCGCTGCCGTGCAATTCGATAGATGACGTATTTAGCAAGGTGGAAGCCGGCAAGGCAAATTATGGGGTTG
TACCGGTAGAGAATTCGACCGAGGGCGCAGTGGGTAGGTCGCTCGATCTGTTGCTGCAAACCCGCTTGAAGGTGTGCGGC
GAAGTGGCGCTGGCCATACATCAGCTTCTGCTGGCGCATCATACCGACCTTGCACGCATTCGCAGGATTTACTCTCATCC
TCAATCGTTTGCTCAATGTCACGAGTGGCTCAATGTCCATTTGCCCCATTTACCCGCGTCGGCAAGAATCAACGCCGCGA
GCAATGCGGATGCTGCCAGACTGGCGGCGGAAGATGAAAGCGCCGCGGCAGTGGCGGGAAAAAAGGCGGGAGAAGTATAT
GGTCTCACTGTCTGTGCCGAGAACATCGAGGATGATCCCAGCAATACGACCCGCTTCCTGGTAATAGGTGAGCAGGAAGT
CGCCCCTTCCGGCAGGGATAAAACGTCGCTGGTAACGTCGGTCAGGAATCGGCCGGGCGCCATACATGAGTTACTGGCCC
CGTTCGCCCATCATGGAGTCAGCATGACCCGGCTTGAATCGCGCCCGTCCCGTGCGGGTTTATGGGAATACGTGTTTTTT
GTGGATGTCGAAGGCCACCAGCAGGAGCCGAAAGTCTCCCAGGCGCTGCGCGAACTGGTGGAAAAAGCGGCGTTTCTCAA
AGTGCTCGGCTCATATCCCGCAGCCTGA

Upstream 100 bases:

>100_bases
GGGTGTGTTGAATGTACGCTACCTGCCGGTACCGGCCAAATAGCTGCCATTTCATGTCATAGCTTTGGCAGGTACGGTTC
AAAACAGAAATTATCCCGCG

Downstream 100 bases:

>100_bases
CCCTCCTCCTCTGTTTTCTCATAAACCTCCATGCGCTGCCGCGAAATGCCGTAAAGTGAATCCCGTGCGATGATCCAGTT
ACCCTGCGAAAAATTCGGCG

Product: chorismate mutase

Products: NA

Alternate protein names: Chorismate mutase; CM; Prephenate dehydratase; PDT [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MTAQLKQLRDKIDAIDSELLKLVSARADLAREIGEIKNGTAYRPEREAQVLARMRELNPGPLENEQVARLFTEIMSLCRS
MEEPLTVAYLGPRGTFSEEAALKRFGSVVTSLPCNSIDDVFSKVEAGKANYGVVPVENSTEGAVGRSLDLLLQTRLKVCG
EVALAIHQLLLAHHTDLARIRRIYSHPQSFAQCHEWLNVHLPHLPASARINAASNADAARLAAEDESAAAVAGKKAGEVY
GLTVCAENIEDDPSNTTRFLVIGEQEVAPSGRDKTSLVTSVRNRPGAIHELLAPFAHHGVSMTRLESRPSRAGLWEYVFF
VDVEGHQQEPKVSQALRELVEKAAFLKVLGSYPAA

Sequences:

>Translated_355_residues
MTAQLKQLRDKIDAIDSELLKLVSARADLAREIGEIKNGTAYRPEREAQVLARMRELNPGPLENEQVARLFTEIMSLCRS
MEEPLTVAYLGPRGTFSEEAALKRFGSVVTSLPCNSIDDVFSKVEAGKANYGVVPVENSTEGAVGRSLDLLLQTRLKVCG
EVALAIHQLLLAHHTDLARIRRIYSHPQSFAQCHEWLNVHLPHLPASARINAASNADAARLAAEDESAAAVAGKKAGEVY
GLTVCAENIEDDPSNTTRFLVIGEQEVAPSGRDKTSLVTSVRNRPGAIHELLAPFAHHGVSMTRLESRPSRAGLWEYVFF
VDVEGHQQEPKVSQALRELVEKAAFLKVLGSYPAA
>Mature_354_residues
TAQLKQLRDKIDAIDSELLKLVSARADLAREIGEIKNGTAYRPEREAQVLARMRELNPGPLENEQVARLFTEIMSLCRSM
EEPLTVAYLGPRGTFSEEAALKRFGSVVTSLPCNSIDDVFSKVEAGKANYGVVPVENSTEGAVGRSLDLLLQTRLKVCGE
VALAIHQLLLAHHTDLARIRRIYSHPQSFAQCHEWLNVHLPHLPASARINAASNADAARLAAEDESAAAVAGKKAGEVYG
LTVCAENIEDDPSNTTRFLVIGEQEVAPSGRDKTSLVTSVRNRPGAIHELLAPFAHHGVSMTRLESRPSRAGLWEYVFFV
DVEGHQQEPKVSQALRELVEKAAFLKVLGSYPAA

Specific function: L-phenylalanine biosynthesis. [C]

COG id: COG0077

COG function: function code E; Prephenate dehydratase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 prephenate dehydratase domain [H]

Homologues:

Organism=Escherichia coli, GI1788951, Length=383, Percent_Identity=34.9869451697128, Blast_Score=195, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6324013, Length=321, Percent_Identity=26.4797507788162, Blast_Score=107, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR008242
- InterPro:   IPR002701
- InterPro:   IPR020822
- InterPro:   IPR010957
- InterPro:   IPR001086
- InterPro:   IPR018528 [H]

Pfam domain/function: PF01842 ACT; PF01817 CM_2; PF00800 PDT [H]

EC number: =5.4.99.5; =4.2.1.51 [H]

Molecular weight: Translated: 38804; Mature: 38672

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00858 PREPHENATE_DEHYDR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQLKQLRDKIDAIDSELLKLVSARADLAREIGEIKNGTAYRPEREAQVLARMRELNPG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCC
PLENEQVARLFTEIMSLCRSMEEPLTVAYLGPRGTFSEEAALKRFGSVVTSLPCNSIDDV
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHH
FSKVEAGKANYGVVPVENSTEGAVGRSLDLLLQTRLKVCGEVALAIHQLLLAHHTDLARI
HHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
RRIYSHPQSFAQCHEWLNVHLPHLPASARINAASNADAARLAAEDESAAAVAGKKAGEVY
HHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHCCCCCCEE
GLTVCAENIEDDPSNTTRFLVIGEQEVAPSGRDKTSLVTSVRNRPGAIHELLAPFAHHGV
EHHEEHHCCCCCCCCCEEEEEEECHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCC
SMTRLESRPSRAGLWEYVFFVDVEGHQQEPKVSQALRELVEKAAFLKVLGSYPAA
CHHHHHCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TAQLKQLRDKIDAIDSELLKLVSARADLAREIGEIKNGTAYRPEREAQVLARMRELNPG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCC
PLENEQVARLFTEIMSLCRSMEEPLTVAYLGPRGTFSEEAALKRFGSVVTSLPCNSIDDV
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHH
FSKVEAGKANYGVVPVENSTEGAVGRSLDLLLQTRLKVCGEVALAIHQLLLAHHTDLARI
HHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
RRIYSHPQSFAQCHEWLNVHLPHLPASARINAASNADAARLAAEDESAAAVAGKKAGEVY
HHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHCCCCCCEE
GLTVCAENIEDDPSNTTRFLVIGEQEVAPSGRDKTSLVTSVRNRPGAIHELLAPFAHHGV
EHHEEHHCCCCCCCCCEEEEEEECHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCC
SMTRLESRPSRAGLWEYVFFVDVEGHQQEPKVSQALRELVEKAAFLKVLGSYPAA
CHHHHHCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1919506; 10368439 [H]