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The map label for this gene is ecaIM [H]

Identifier: 82703297

GI number: 82703297

Start: 2473854

End: 2475875

Strand: Reverse

Name: ecaIM [H]

Synonym: Nmul_A2179

Alternate gene names: 82703297

Gene position: 2475875-2473854 (Counterclockwise)

Preceding gene: 82703298

Following gene: 82703296

Centisome position: 77.75

GC content: 49.75

Gene sequence:

>2022_bases
ATGACACCTGAACAACGACAACGCATCATCGAAATCCTACTTTCCGGTGGAGAAGTAGCGCCGGAATGGTCGCGCATCCT
GTTCCCGCCTGAGAAGCGTGAATACGAGCTGGTCTATCAAGGCAAGGAGCGCGAGGAAGACATCATCGCCAACACGCTGG
CAGTACCCTTACAGCCAGTACGAACCTTCAATAAAAACGGGGTGACGTGGCACAACAAATTGATCTTTGGGGACAACCTG
CAAGCCATGAAGACGTTGCTGGAAATGAAGCGGCGTGGGGAACTATGCAACGCCGACGGAACGTCTGGTATCCGCTTGGT
ATATATCGACCCGCCATTTGCAACTCGACAAGAATTTCAGGGCGCGCAAGACCAAAAAGCGTATCAGGATAAGATTTATG
GCGCTACATTTATTGAGTTTTTGCGAAAAAGGCTAATCCTCATTCGTGACCTTCTTTCTGATAATGGCCTTCTATACGTT
CACCTGGATTACCGTAAGTCTCATTACATAAAAGTTATCCTTGATGAGATTTTTGGCGAGCAAAACTTTATGAACGAAGT
TGCTTGGTGCTACGGAGAACGTGAGTTAGCAACACGTCATTGGAACAGGAAGCATGACAATATCTTGGTGTATGCAAAAA
ACTTCAAATCAGATCAACATGTATTTAACTGGAAAGAGGCGGCTGGTCAGTATTCACAAGGTACTTTGGCAAAATATGAG
CACATCGATGAAGATGGAAGGAAATTTCAATTAAGGGGAAGAAACGTCAAGGGAAGTCCATGGCGCGGTAAGCATGGTAT
TCCTTTGGATGTAGAGGCAGCAAATCCGGAATGGGTCTATAGGGATTACTTCGACACAAAAGAGGGTATTAGACCTCGTG
ATTGGTGGAGCGACATTCCATTTCTTAACAGAGCATCAAGTGACAGATACGACTATCCAAGCGCGAAAAATCCTGCACTT
TTAAATAGAATCATCAAGGTCTCCTCCAATATTGGAGATCTTGTTATGGATGCATTTGCCGGATCAGGAACCACCTGTGC
GGTTGCAGAAAAACTTAATCGTCGCTGGATCGGCATTGACTGCGGGAAACTTGCGATCTACACCATCCAGAAGCGGATGC
TGAATCTCAGCGAAAAGGGCAAGGCGCTCAAAGCCAAGCCCTTCACCCTTTACAACGCCGGCCTCTACGACTTCGCCCGT
CTTAAGGAATTGCCTTGGAGTGACTGGCGGCGGTTTGCCCTCACCCTCTTTAGTTGCCAGAGTGCCCCCCAACGAATCGG
TGGCATCCAATTTGATGGCACACTGAAAGCCAGCCCCGTCATTATTTTCGATCACCGCAAGGGGAATGGCGCGACAGTCA
GCGAGGAGACGTTACGCTCTATCCATGAAGCGGCGGGCTCCAAGGTTGGCACACGTATATTCATTATCGCGCCGGCAATG
GCCTTCGACTTCCAACAGGATTACATCCAGATCGGGGAGGTACGCTATTACGCTCTGCGGGTGCCGTATTCCATCATTCA
CGAGCTGCATCAACGCGACTTTCTTGCCTTAAAACAGCCGACTGACGAGATGGCGGTGAACGACACGGTAGATGCCGTAG
GCTTCGATTTCATTAAGACCCCTGAGTTGGAGTACGCCGTGGGTCGAGGCAACCCGGAGGGTGAATTGCTTGAGCAAGCA
TTCATTCGCATCGACACTTTTTTCAGCGAGGCCGCCGTGCGGGAGCCGATGCGAAAACGTGGCAACCGGGAAACATTGTC
CATGGTAATGTTGGATTATGACTACGATGCCGAGAATGATGTGTTCGATCTGGACGAGGTGTTTTATGCCGAAGCTATCG
AGGGGGCGGCTTGGGAGGTGCGTTTTCCTGCCAACCGCCCCGGCGAGAAGGTCATGGCGGTTTTCCTAGACATCTATGGC
AATGAGGCGCGTGTGGTGATACCTGCGGCTCATTTTGCGCTAGAGAAACTTGAAAAGCGCAACGGCTCTCCCATTGCAAA
GACAGAGGCAAAAAGAGCATGA

Upstream 100 bases:

>100_bases
TGTTACCGATTAAAAATGCTAGCCTGATGAAGGCCTGTAATAGTCGTTATATAGAACGCCAAGAGGTGCAGGCGCTGAAA
ACGAAGCTGAAAAATTTAAC

Downstream 100 bases:

>100_bases
GGACTACCGCACGCCATTTCGGTGACCGCCGCGCCTTCCGCAATGAAGACCTGGTTCTGGCAATCAAGCCCCATGATCCT
TGTAAATGGGATGAGAGCCG

Product: adenine-specific DNA-methyltransferase

Products: NA

Alternate protein names: M.EcaI; Adenine-specific methyltransferase EcaI [H]

Number of amino acids: Translated: 673; Mature: 672

Protein sequence:

>673_residues
MTPEQRQRIIEILLSGGEVAPEWSRILFPPEKREYELVYQGKEREEDIIANTLAVPLQPVRTFNKNGVTWHNKLIFGDNL
QAMKTLLEMKRRGELCNADGTSGIRLVYIDPPFATRQEFQGAQDQKAYQDKIYGATFIEFLRKRLILIRDLLSDNGLLYV
HLDYRKSHYIKVILDEIFGEQNFMNEVAWCYGERELATRHWNRKHDNILVYAKNFKSDQHVFNWKEAAGQYSQGTLAKYE
HIDEDGRKFQLRGRNVKGSPWRGKHGIPLDVEAANPEWVYRDYFDTKEGIRPRDWWSDIPFLNRASSDRYDYPSAKNPAL
LNRIIKVSSNIGDLVMDAFAGSGTTCAVAEKLNRRWIGIDCGKLAIYTIQKRMLNLSEKGKALKAKPFTLYNAGLYDFAR
LKELPWSDWRRFALTLFSCQSAPQRIGGIQFDGTLKASPVIIFDHRKGNGATVSEETLRSIHEAAGSKVGTRIFIIAPAM
AFDFQQDYIQIGEVRYYALRVPYSIIHELHQRDFLALKQPTDEMAVNDTVDAVGFDFIKTPELEYAVGRGNPEGELLEQA
FIRIDTFFSEAAVREPMRKRGNRETLSMVMLDYDYDAENDVFDLDEVFYAEAIEGAAWEVRFPANRPGEKVMAVFLDIYG
NEARVVIPAAHFALEKLEKRNGSPIAKTEAKRA

Sequences:

>Translated_673_residues
MTPEQRQRIIEILLSGGEVAPEWSRILFPPEKREYELVYQGKEREEDIIANTLAVPLQPVRTFNKNGVTWHNKLIFGDNL
QAMKTLLEMKRRGELCNADGTSGIRLVYIDPPFATRQEFQGAQDQKAYQDKIYGATFIEFLRKRLILIRDLLSDNGLLYV
HLDYRKSHYIKVILDEIFGEQNFMNEVAWCYGERELATRHWNRKHDNILVYAKNFKSDQHVFNWKEAAGQYSQGTLAKYE
HIDEDGRKFQLRGRNVKGSPWRGKHGIPLDVEAANPEWVYRDYFDTKEGIRPRDWWSDIPFLNRASSDRYDYPSAKNPAL
LNRIIKVSSNIGDLVMDAFAGSGTTCAVAEKLNRRWIGIDCGKLAIYTIQKRMLNLSEKGKALKAKPFTLYNAGLYDFAR
LKELPWSDWRRFALTLFSCQSAPQRIGGIQFDGTLKASPVIIFDHRKGNGATVSEETLRSIHEAAGSKVGTRIFIIAPAM
AFDFQQDYIQIGEVRYYALRVPYSIIHELHQRDFLALKQPTDEMAVNDTVDAVGFDFIKTPELEYAVGRGNPEGELLEQA
FIRIDTFFSEAAVREPMRKRGNRETLSMVMLDYDYDAENDVFDLDEVFYAEAIEGAAWEVRFPANRPGEKVMAVFLDIYG
NEARVVIPAAHFALEKLEKRNGSPIAKTEAKRA
>Mature_672_residues
TPEQRQRIIEILLSGGEVAPEWSRILFPPEKREYELVYQGKEREEDIIANTLAVPLQPVRTFNKNGVTWHNKLIFGDNLQ
AMKTLLEMKRRGELCNADGTSGIRLVYIDPPFATRQEFQGAQDQKAYQDKIYGATFIEFLRKRLILIRDLLSDNGLLYVH
LDYRKSHYIKVILDEIFGEQNFMNEVAWCYGERELATRHWNRKHDNILVYAKNFKSDQHVFNWKEAAGQYSQGTLAKYEH
IDEDGRKFQLRGRNVKGSPWRGKHGIPLDVEAANPEWVYRDYFDTKEGIRPRDWWSDIPFLNRASSDRYDYPSAKNPALL
NRIIKVSSNIGDLVMDAFAGSGTTCAVAEKLNRRWIGIDCGKLAIYTIQKRMLNLSEKGKALKAKPFTLYNAGLYDFARL
KELPWSDWRRFALTLFSCQSAPQRIGGIQFDGTLKASPVIIFDHRKGNGATVSEETLRSIHEAAGSKVGTRIFIIAPAMA
FDFQQDYIQIGEVRYYALRVPYSIIHELHQRDFLALKQPTDEMAVNDTVDAVGFDFIKTPELEYAVGRGNPEGELLEQAF
IRIDTFFSEAAVREPMRKRGNRETLSMVMLDYDYDAENDVFDLDEVFYAEAIEGAAWEVRFPANRPGEKVMAVFLDIYGN
EARVVIPAAHFALEKLEKRNGSPIAKTEAKRA

Specific function: This methylase recognizes the double-stranded sequence GGTNACC, causes specific methylation on A-5 on both strands and protects the DNA from cleavage by the EcaI endonuclease [H]

COG id: COG2189

COG function: function code L; Adenine specific DNA methylase Mod

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002295
- InterPro:   IPR002941
- InterPro:   IPR002052 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 77296; Mature: 77165

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPEQRQRIIEILLSGGEVAPEWSRILFPPEKREYELVYQGKEREEDIIANTLAVPLQPV
CCHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCEEEEECCCCCCHHHHHHHHHCCCHHH
RTFNKNGVTWHNKLIFGDNLQAMKTLLEMKRRGELCNADGTSGIRLVYIDPPFATRQEFQ
HHCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHC
GAQDQKAYQDKIYGATFIEFLRKRLILIRDLLSDNGLLYVHLDYRKSHYIKVILDEIFGE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCC
QNFMNEVAWCYGERELATRHWNRKHDNILVYAKNFKSDQHVFNWKEAAGQYSQGTLAKYE
HHHHHHHHHHCCCHHHHHHHCCCCCCCEEEEEECCCCCCHHCCHHHHCCCCCCCCHHHHH
HIDEDGRKFQLRGRNVKGSPWRGKHGIPLDVEAANPEWVYRDYFDTKEGIRPRDWWSDIP
HCCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCCCHHHCCCC
FLNRASSDRYDYPSAKNPALLNRIIKVSSNIGDLVMDAFAGSGTTCAVAEKLNRRWIGID
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCEEHHHHHHCCEEEEEE
CGKLAIYTIQKRMLNLSEKGKALKAKPFTLYNAGLYDFARLKELPWSDWRRFALTLFSCQ
CCHHHHHHHHHHHHCHHHCCCEEECCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHH
SAPQRIGGIQFDGTLKASPVIIFDHRKGNGATVSEETLRSIHEAAGSKVGTRIFIIAPAM
CCHHHHCCEEECCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECHH
AFDFQQDYIQIGEVRYYALRVPYSIIHELHQRDFLALKQPTDEMAVNDTVDAVGFDFIKT
HHHHHHHHHHHCCEEEEEEECCHHHHHHHHHCCCEEECCCCHHHHCCCCHHHCCCCCCCC
PELEYAVGRGNPEGELLEQAFIRIDTFFSEAAVREPMRKRGNRETLSMVMLDYDYDAEND
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCC
VFDLDEVFYAEAIEGAAWEVRFPANRPGEKVMAVFLDIYGNEARVVIPAAHFALEKLEKR
CCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHC
NGSPIAKTEAKRA
CCCCCCCCCCCCC
>Mature Secondary Structure 
TPEQRQRIIEILLSGGEVAPEWSRILFPPEKREYELVYQGKEREEDIIANTLAVPLQPV
CHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCEEEEECCCCCCHHHHHHHHHCCCHHH
RTFNKNGVTWHNKLIFGDNLQAMKTLLEMKRRGELCNADGTSGIRLVYIDPPFATRQEFQ
HHCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHC
GAQDQKAYQDKIYGATFIEFLRKRLILIRDLLSDNGLLYVHLDYRKSHYIKVILDEIFGE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCC
QNFMNEVAWCYGERELATRHWNRKHDNILVYAKNFKSDQHVFNWKEAAGQYSQGTLAKYE
HHHHHHHHHHCCCHHHHHHHCCCCCCCEEEEEECCCCCCHHCCHHHHCCCCCCCCHHHHH
HIDEDGRKFQLRGRNVKGSPWRGKHGIPLDVEAANPEWVYRDYFDTKEGIRPRDWWSDIP
HCCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCCCHHHCCCC
FLNRASSDRYDYPSAKNPALLNRIIKVSSNIGDLVMDAFAGSGTTCAVAEKLNRRWIGID
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCEEHHHHHHCCEEEEEE
CGKLAIYTIQKRMLNLSEKGKALKAKPFTLYNAGLYDFARLKELPWSDWRRFALTLFSCQ
CCHHHHHHHHHHHHCHHHCCCEEECCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHH
SAPQRIGGIQFDGTLKASPVIIFDHRKGNGATVSEETLRSIHEAAGSKVGTRIFIIAPAM
CCHHHHCCEEECCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECHH
AFDFQQDYIQIGEVRYYALRVPYSIIHELHQRDFLALKQPTDEMAVNDTVDAVGFDFIKT
HHHHHHHHHHHCCEEEEEEECCHHHHHHHHHCCCEEECCCCHHHHCCCCHHHCCCCCCCC
PELEYAVGRGNPEGELLEQAFIRIDTFFSEAAVREPMRKRGNRETLSMVMLDYDYDAEND
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCC
VFDLDEVFYAEAIEGAAWEVRFPANRPGEKVMAVFLDIYGNEARVVIPAAHFALEKLEKR
CCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHC
NGSPIAKTEAKRA
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2183182 [H]