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The map label for this gene is rtcA

Identifier: 82703277

GI number: 82703277

Start: 2452427

End: 2453488

Strand: Reverse

Name: rtcA

Synonym: Nmul_A2158

Alternate gene names: 82703277

Gene position: 2453488-2452427 (Counterclockwise)

Preceding gene: 82703284

Following gene: 82703276

Centisome position: 77.05

GC content: 57.72

Gene sequence:

>1062_bases
ATGCAGGAAATCGATGGTTCTTATGGAGAAGGTGGCGGCCAGTTGCTCCGCACGTCGGTTGCGCTTGCGGCGATAACGGG
ACAATCGGTTCGTGTGTACAACATTCGTGCAAAACGTTCCAATCCTGGTCTTGCGCCTCAACATCTGACTGCCGTAAAGG
CGGTGGCAGCGCTTTGCAGGGCCCGGACGGAAGGAATGGAAGTCAAATCGCAGGAAATCATTTTTCGCCCTGGCCCGTTA
CGCGGGGGCGAATATGATTTTCCGATAGGCACGGCAGGCAGTGTTACCTTGGTGCTCCAGGCAGCGCTTCCAGTTGCCTT
GGCATGCGGAGAAAAGGTGCGGATGAACATTTCGGGTGGGACCGATGTTCGCGCTGCGCCGCCCCTGGATTACTTCCGCT
ACGTATTGCTGCCGCTGGTTTATAGCATGGGCGCCAGGGCGAAGATCGAAGTGTTGCTCCGGGGGTATTATCCTCGCGGC
GGAGGAAAGGTGGTTGTGGACGTAGAACCTTGCCTGCCTTTGCGTCCGGTGCTCCTGAACGCATCGGAAGGGCTGGAGGG
TATAACCGGTTTCGTACACATTTCAAACCTGCCCAAGCACATCATCCACCGCATGGCGAACGGAGCACTGGCGGAACTTT
CGACTTTTCCCACCCCAGCTGTTGGCCTGGAAGTATTCGGGAAGGATGACGCGATAGGTGAGGGCGGAGCGGTGCTTTTG
ACCGCGCACAAGGAGCATAGCCGTCTGGGGGCATCTGCCGTCGCAGAAAGAGGCGTGCCAGCCGAACGCCTCGGTGCTGA
GGCGGGGCGGTGCTTGCGCGAGGAAATCCTGTCCGGCGCAACGCTGGATATTCATGCGGCAGACCAAGTATTGATCTACC
TCGCGCTGGCGAGCGGCGTATCTTGCTTTCTCACAAGGGAACTCTCCTCCCACGCCGCGACAACCATTTGGCTGCTGGAA
CAGTTTCTGCCAGTCCGCTTCCAGGTCACACAGGAGGCGCATTTGATTCGCGTCCGCGCAAAGCCGGAATTTAATGGTAT
GTCAAGCTTTTTGTGGCGATAA

Upstream 100 bases:

>100_bases
AGGCAGATGCCCAGCCGCGCGGTCATGGGATTCTTGATGCTACATGAATTGTAGGTCGAAAAATCTATTGTTCCCGGCTA
TATCAACCAAAGAGGTCAGA

Downstream 100 bases:

>100_bases
GAGGATTTGACGGGGGAAACGTCCTACTACTGTTATTCGAACCGTCCCTGCCTTCCCTTGCCCTGGGCTCCCAAGCCACA
TGGACCCGGCTTCAACGGGG

Product: RNA 3'-terminal-phosphate cyclase

Products: NA

Alternate protein names: RNA cyclase; RNA-3'-phosphate cyclase

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MQEIDGSYGEGGGQLLRTSVALAAITGQSVRVYNIRAKRSNPGLAPQHLTAVKAVAALCRARTEGMEVKSQEIIFRPGPL
RGGEYDFPIGTAGSVTLVLQAALPVALACGEKVRMNISGGTDVRAAPPLDYFRYVLLPLVYSMGARAKIEVLLRGYYPRG
GGKVVVDVEPCLPLRPVLLNASEGLEGITGFVHISNLPKHIIHRMANGALAELSTFPTPAVGLEVFGKDDAIGEGGAVLL
TAHKEHSRLGASAVAERGVPAERLGAEAGRCLREEILSGATLDIHAADQVLIYLALASGVSCFLTRELSSHAATTIWLLE
QFLPVRFQVTQEAHLIRVRAKPEFNGMSSFLWR

Sequences:

>Translated_353_residues
MQEIDGSYGEGGGQLLRTSVALAAITGQSVRVYNIRAKRSNPGLAPQHLTAVKAVAALCRARTEGMEVKSQEIIFRPGPL
RGGEYDFPIGTAGSVTLVLQAALPVALACGEKVRMNISGGTDVRAAPPLDYFRYVLLPLVYSMGARAKIEVLLRGYYPRG
GGKVVVDVEPCLPLRPVLLNASEGLEGITGFVHISNLPKHIIHRMANGALAELSTFPTPAVGLEVFGKDDAIGEGGAVLL
TAHKEHSRLGASAVAERGVPAERLGAEAGRCLREEILSGATLDIHAADQVLIYLALASGVSCFLTRELSSHAATTIWLLE
QFLPVRFQVTQEAHLIRVRAKPEFNGMSSFLWR
>Mature_353_residues
MQEIDGSYGEGGGQLLRTSVALAAITGQSVRVYNIRAKRSNPGLAPQHLTAVKAVAALCRARTEGMEVKSQEIIFRPGPL
RGGEYDFPIGTAGSVTLVLQAALPVALACGEKVRMNISGGTDVRAAPPLDYFRYVLLPLVYSMGARAKIEVLLRGYYPRG
GGKVVVDVEPCLPLRPVLLNASEGLEGITGFVHISNLPKHIIHRMANGALAELSTFPTPAVGLEVFGKDDAIGEGGAVLL
TAHKEHSRLGASAVAERGVPAERLGAEAGRCLREEILSGATLDIHAADQVLIYLALASGVSCFLTRELSSHAATTIWLLE
QFLPVRFQVTQEAHLIRVRAKPEFNGMSSFLWR

Specific function: Catalyzes the conversion of 3'-phosphate to a 2',3'- cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps:(A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA- N3'PP5'A;

COG id: COG0430

COG function: function code A; RNA 3'-terminal phosphate cyclase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4506589, Length=334, Percent_Identity=35.6287425149701, Blast_Score=198, Evalue=5e-51,
Organism=Homo sapiens, GI195927044, Length=347, Percent_Identity=34.2939481268012, Blast_Score=190, Evalue=2e-48,
Organism=Escherichia coli, GI48994937, Length=339, Percent_Identity=38.0530973451327, Blast_Score=210, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI32564710, Length=331, Percent_Identity=23.8670694864048, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324564, Length=294, Percent_Identity=22.108843537415, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI18543269, Length=337, Percent_Identity=35.6083086053413, Blast_Score=187, Evalue=7e-48,
Organism=Drosophila melanogaster, GI18860065, Length=334, Percent_Identity=21.5568862275449, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RTCA_NITMU (Q2Y720)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_412843.1
- ProteinModelPortal:   Q2Y720
- SMR:   Q2Y720
- STRING:   Q2Y720
- GeneID:   3784398
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2158
- eggNOG:   COG0430
- HOGENOM:   HBG683539
- OMA:   EIDGSYG
- PhylomeDB:   Q2Y720
- ProtClustDB:   PRK04204
- BioCyc:   NMUL323848:NMUL_A2158-MONOMER
- GO:   GO:0005737
- GO:   GO:0005654
- HAMAP:   MF_00200
- InterPro:   IPR013791
- InterPro:   IPR000228
- InterPro:   IPR020719
- InterPro:   IPR013796
- InterPro:   IPR020723
- InterPro:   IPR017770
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.20
- PANTHER:   PTHR11096
- TIGRFAMs:   TIGR03399

Pfam domain/function: PF01137 RTC; PF05189 RTC_insert; SSF52913 RNA3'-term_phos_cycl_insert; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =6.5.1.4

Molecular weight: Translated: 37750; Mature: 37750

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS01287 RTC

Important sites: ACT_SITE 311-311 BINDING 100-100

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEIDGSYGEGGGQLLRTSVALAAITGQSVRVYNIRAKRSNPGLAPQHLTAVKAVAALCR
CCCCCCCCCCCCHHHHHHHHHHEEECCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHH
ARTEGMEVKSQEIIFRPGPLRGGEYDFPIGTAGSVTLVLQAALPVALACGEKVRMNISGG
HHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCC
TDVRAAPPLDYFRYVLLPLVYSMGARAKIEVLLRGYYPRGGGKVVVDVEPCLPLRPVLLN
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCCEEEE
ASEGLEGITGFVHISNLPKHIIHRMANGALAELSTFPTPAVGLEVFGKDDAIGEGGAVLL
CCCCCCCCEEEEEECCCHHHHHHHHHCCHHHHHHCCCCCCCCEEEECCCCCCCCCCEEEE
TAHKEHSRLGASAVAERGVPAERLGAEAGRCLREEILSGATLDIHAADQVLIYLALASGV
EECCHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHCCC
SCFLTRELSSHAATTIWLLEQFLPVRFQVTQEAHLIRVRAKPEFNGMSSFLWR
EEEEEHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEEEECCCCCCCHHHHHCC
>Mature Secondary Structure
MQEIDGSYGEGGGQLLRTSVALAAITGQSVRVYNIRAKRSNPGLAPQHLTAVKAVAALCR
CCCCCCCCCCCCHHHHHHHHHHEEECCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHH
ARTEGMEVKSQEIIFRPGPLRGGEYDFPIGTAGSVTLVLQAALPVALACGEKVRMNISGG
HHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCC
TDVRAAPPLDYFRYVLLPLVYSMGARAKIEVLLRGYYPRGGGKVVVDVEPCLPLRPVLLN
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCCEEEE
ASEGLEGITGFVHISNLPKHIIHRMANGALAELSTFPTPAVGLEVFGKDDAIGEGGAVLL
CCCCCCCCEEEEEECCCHHHHHHHHHCCHHHHHHCCCCCCCCEEEECCCCCCCCCCEEEE
TAHKEHSRLGASAVAERGVPAERLGAEAGRCLREEILSGATLDIHAADQVLIYLALASGV
EECCHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHCCC
SCFLTRELSSHAATTIWLLEQFLPVRFQVTQEAHLIRVRAKPEFNGMSSFLWR
EEEEEHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEEEECCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA