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The map label for this gene is anfA [H]

Identifier: 82703247

GI number: 82703247

Start: 2422046

End: 2423020

Strand: Direct

Name: anfA [H]

Synonym: Nmul_A2128

Alternate gene names: 82703247

Gene position: 2422046-2423020 (Clockwise)

Preceding gene: 82703243

Following gene: 82703253

Centisome position: 76.06

GC content: 54.36

Gene sequence:

>975_bases
ATGATTGGACAATCCCCTGCCTTCGCAAAGGTTACCCAGTTCATTGAGAAAATTGCAGGCTGCGACGCACCTGTTCTTAT
CGAAGGTGAAACAGGCACAGGAAAAGAACTGGCCGCACGGGCAATCCACGACAGCGGCGCGAGGCGAAACGGGGCGTTTG
TGCCGATAAATTGCCGCGCCATCCCGGATACCCTGATTGAAAACGAACTGTTCGGACACGAACGGGATTCCTGTATCGGA
GGCTCGCCGGAACACCCGAATCCGAGTGCCCCCGCGCAGTCCGGCACACTCTTCCTGGATGAAGCAGATGCGTTATCTCC
CAAGGCGCAGGTTACTCTGCTGCGATTCTTCCAGGATCAGGAATACCTTCCGCTGGGACGCCGCATAGCGCGCTCTGCAA
ACAGAAGAATAATTATTGCCAGTAACAGAAACTTAGGGCACTTGGCCGAGAAAGGCGAATTCCGCCTTGATCTTCTGTTT
CGCCTTAAAATCATGTATCTCACCCTGCCGCCACTCCGTGAGCGCCAAGGCGACGCGGCTCTGTTAGCAGATTACTTCCT
GCGCGAATGCTCCGCGCGTTTTAACCGCGGCAACAAAACCTTACATCCGAACACTGTCGCATGGTTCGACAAATACAATT
GGCCCGGAAATATACGGGAACTGGAAAATCTGATCTATCGCGAGTATCTTCTCGCCGACGAAGATGTCATAAATATCGCA
TCGCTCGCGCCCACAGTGGCCGAACGGCGCTCCGGGTTTGACCGCCGCCTCCCCGAGCTGAACGGCTTGAGCTTCGCCGA
GGCCAAAAACCGCGCCATAGCAGCTTTCGAACAATATTACCTTGCCGAGGCCATGGTCAGAGCGCAGGGTAACGTGACAA
GAGCTGCGAGCCTTGTCGGAAAAGATCGACGCGCGTTCGGGAAATTGCTGAAAAAGCATAGTATGAGCAAGAACAAGCTC
TTTCTGGAACACTGA

Upstream 100 bases:

>100_bases
TAATAGTTATACCACGGGGCCATGCAGAAAGATGAGTTCCACAGGCTTTTTTGAATCCCTTACGTAAGAATCGATCTTCC
CGCTTCCAGGTGCAGAGAAC

Downstream 100 bases:

>100_bases
TTCTTCCCTCTTCAGGTTTCCTCTCCAAATTTTCGCGCCGGCACTCGATTCCCTGTCTGCCGTCGACGAGGCACGTATTC
GACAGAACCTGCAGGGCGTT

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MIGQSPAFAKVTQFIEKIAGCDAPVLIEGETGTGKELAARAIHDSGARRNGAFVPINCRAIPDTLIENELFGHERDSCIG
GSPEHPNPSAPAQSGTLFLDEADALSPKAQVTLLRFFQDQEYLPLGRRIARSANRRIIIASNRNLGHLAEKGEFRLDLLF
RLKIMYLTLPPLRERQGDAALLADYFLRECSARFNRGNKTLHPNTVAWFDKYNWPGNIRELENLIYREYLLADEDVINIA
SLAPTVAERRSGFDRRLPELNGLSFAEAKNRAIAAFEQYYLAEAMVRAQGNVTRAASLVGKDRRAFGKLLKKHSMSKNKL
FLEH

Sequences:

>Translated_324_residues
MIGQSPAFAKVTQFIEKIAGCDAPVLIEGETGTGKELAARAIHDSGARRNGAFVPINCRAIPDTLIENELFGHERDSCIG
GSPEHPNPSAPAQSGTLFLDEADALSPKAQVTLLRFFQDQEYLPLGRRIARSANRRIIIASNRNLGHLAEKGEFRLDLLF
RLKIMYLTLPPLRERQGDAALLADYFLRECSARFNRGNKTLHPNTVAWFDKYNWPGNIRELENLIYREYLLADEDVINIA
SLAPTVAERRSGFDRRLPELNGLSFAEAKNRAIAAFEQYYLAEAMVRAQGNVTRAASLVGKDRRAFGKLLKKHSMSKNKL
FLEH
>Mature_324_residues
MIGQSPAFAKVTQFIEKIAGCDAPVLIEGETGTGKELAARAIHDSGARRNGAFVPINCRAIPDTLIENELFGHERDSCIG
GSPEHPNPSAPAQSGTLFLDEADALSPKAQVTLLRFFQDQEYLPLGRRIARSANRRIIIASNRNLGHLAEKGEFRLDLLF
RLKIMYLTLPPLRERQGDAALLADYFLRECSARFNRGNKTLHPNTVAWFDKYNWPGNIRELENLIYREYLLADEDVINIA
SLAPTVAERRSGFDRRLPELNGLSFAEAKNRAIAAFEQYYLAEAMVRAQGNVTRAASLVGKDRRAFGKLLKKHSMSKNKL
FLEH

Specific function: AnfA is essential for nitrogen fixation under Mo- and V- deficient conditions. It is required for the regulation of nitrogenase 3 transcription. Interacts with sigma-54 [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=321, Percent_Identity=35.5140186915888, Blast_Score=197, Evalue=9e-52,
Organism=Escherichia coli, GI1788905, Length=318, Percent_Identity=36.1635220125786, Blast_Score=194, Evalue=8e-51,
Organism=Escherichia coli, GI1789233, Length=291, Percent_Identity=36.7697594501718, Blast_Score=184, Evalue=5e-48,
Organism=Escherichia coli, GI1790437, Length=307, Percent_Identity=35.1791530944625, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI87082117, Length=254, Percent_Identity=40.9448818897638, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1789087, Length=240, Percent_Identity=41.25, Blast_Score=178, Evalue=4e-46,
Organism=Escherichia coli, GI1790299, Length=332, Percent_Identity=34.6385542168675, Blast_Score=174, Evalue=6e-45,
Organism=Escherichia coli, GI87082152, Length=320, Percent_Identity=34.6875, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI87081872, Length=330, Percent_Identity=33.9393939393939, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI1786524, Length=324, Percent_Identity=36.4197530864198, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1787583, Length=329, Percent_Identity=31.306990881459, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1789828, Length=283, Percent_Identity=30.7420494699647, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI87081858, Length=316, Percent_Identity=26.5822784810127, Blast_Score=104, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 36264; Mature: 36264

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGQSPAFAKVTQFIEKIAGCDAPVLIEGETGTGKELAARAIHDSGARRNGAFVPINCRA
CCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEECCC
IPDTLIENELFGHERDSCIGGSPEHPNPSAPAQSGTLFLDEADALSPKAQVTLLRFFQDQ
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCC
EYLPLGRRIARSANRRIIIASNRNLGHLAEKGEFRLDLLFRLKIMYLTLPPLRERQGDAA
CCCHHHHHHHHCCCCEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCHHHCCCCHH
LLADYFLRECSARFNRGNKTLHPNTVAWFDKYNWPGNIRELENLIYREYLLADEDVINIA
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHH
SLAPTVAERRSGFDRRLPELNGLSFAEAKNRAIAAFEQYYLAEAMVRAQGNVTRAASLVG
HHHHHHHHHHCCHHHCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KDRRAFGKLLKKHSMSKNKLFLEH
HHHHHHHHHHHHHCCCCCCEEECC
>Mature Secondary Structure
MIGQSPAFAKVTQFIEKIAGCDAPVLIEGETGTGKELAARAIHDSGARRNGAFVPINCRA
CCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEECCC
IPDTLIENELFGHERDSCIGGSPEHPNPSAPAQSGTLFLDEADALSPKAQVTLLRFFQDQ
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCC
EYLPLGRRIARSANRRIIIASNRNLGHLAEKGEFRLDLLFRLKIMYLTLPPLRERQGDAA
CCCHHHHHHHHCCCCEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCHHHCCCCHH
LLADYFLRECSARFNRGNKTLHPNTVAWFDKYNWPGNIRELENLIYREYLLADEDVINIA
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHH
SLAPTVAERRSGFDRRLPELNGLSFAEAKNRAIAAFEQYYLAEAMVRAQGNVTRAASLVG
HHHHHHHHHHCCHHHCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KDRRAFGKLLKKHSMSKNKLFLEH
HHHHHHHHHHHHHCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2722750 [H]