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The map label for this gene is birA [H]

Identifier: 82703202

GI number: 82703202

Start: 2374239

End: 2375228

Strand: Reverse

Name: birA [H]

Synonym: Nmul_A2082

Alternate gene names: 82703202

Gene position: 2375228-2374239 (Counterclockwise)

Preceding gene: 82703208

Following gene: 82703201

Centisome position: 74.59

GC content: 52.73

Gene sequence:

>990_bases
ATGAATCCATTCACTTTTTCCATTCTCCGGGTGCTGAGCGATAACGAATTTCATTCAGGGCAGGCTATCGCCGAAGCTCT
GGGAGTTTCCCGCGCCAGTGTATCGAATGCTCTTCGCGACGCGGATGAAGCTGGATTGACCATTCATAAAATCAAGGGAC
GCGGCTATCGCCTGCTCGACCAGGTGCAATGGCTGGAACGAAATGCAATTCTTGAGCACCTCGGTCATCAGGCGGACAAA
TTCAATCTGGAAATACTCGATACGATCGATTCCACCAACAGCCTTTTACTGCATGAGGCGGATAACCGGTTGAGCCTGCG
TGATGGGCTCATTCATGTGGTAGCGGCCGAGCTGCAAACGAAGGGGCGTGGACGACGAGGACGGCAATGGCACTCGGGCC
TGGGAGTCGGTCTCGCGTTTTCCGTGCTATGGCGGTTTCAGCAAAGTGCAAGCTTTCTTTCGGGTTTGAGTCTCGCCACA
GGCGTTGCAATAGTACGTGCGCTCGAATCTTCAGGGATACAAGGGGCCGTACTCAAATGGCCAAACGATGTGATGTTCAA
TTTCTGTAAACTGGCAGGTATATTGATAGAACTGCATGGCGATATGCTCGGTCCCACCGTTGCTGTAATCGGTGTAGGCA
TGAACCTGAAATTGTCCGACAGCGTTCAGGCGCGGATAGACCAGGGGGCAACGGATATTTTTTCCATCAGTGGAGAAACA
CCGGATCGCAATAAATTGCTGGCTGAATTGTTGCTGAATATTGCTCGAGTATTGAGAGAATTTGAGCAGTCGGGTTTTAC
GCCATTCAAGGAGGAATGGGTGGATCGCCATGTATGTGAAGGCAAAGCCGTCACCCTCAAGCTACCTGACGGGTCGGGCC
AGGAAGGACTGGTGCACGGGGTATCGGATAGCGGGTCGCTGTTGCTGCAAACGTCACTGGGTCTTCGCAGTTTCAGCGGC
GGCGAGATATCGCTGCGCAGGACAGCATAA

Upstream 100 bases:

>100_bases
CCAGGTACCCCTGCTGACTTTCTTCCTGCATTTCTTTTACTGTGAGCGAGAATGCGCGTGGCAAAGTTCCATTTCCGTTT
ATTTACACTACACTGATTAC

Downstream 100 bases:

>100_bases
ATGAACCCTCTTCTGCTGGCGGTGGATTCCGGCAACACGCGCGTGAAGTGGGGGCTGCATGATGGCCGCAACTGGCTCAT
GCAAGGGGTCGCGGCGCAGG

Product: biotin--acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MNPFTFSILRVLSDNEFHSGQAIAEALGVSRASVSNALRDADEAGLTIHKIKGRGYRLLDQVQWLERNAILEHLGHQADK
FNLEILDTIDSTNSLLLHEADNRLSLRDGLIHVVAAELQTKGRGRRGRQWHSGLGVGLAFSVLWRFQQSASFLSGLSLAT
GVAIVRALESSGIQGAVLKWPNDVMFNFCKLAGILIELHGDMLGPTVAVIGVGMNLKLSDSVQARIDQGATDIFSISGET
PDRNKLLAELLLNIARVLREFEQSGFTPFKEEWVDRHVCEGKAVTLKLPDGSGQEGLVHGVSDSGSLLLQTSLGLRSFSG
GEISLRRTA

Sequences:

>Translated_329_residues
MNPFTFSILRVLSDNEFHSGQAIAEALGVSRASVSNALRDADEAGLTIHKIKGRGYRLLDQVQWLERNAILEHLGHQADK
FNLEILDTIDSTNSLLLHEADNRLSLRDGLIHVVAAELQTKGRGRRGRQWHSGLGVGLAFSVLWRFQQSASFLSGLSLAT
GVAIVRALESSGIQGAVLKWPNDVMFNFCKLAGILIELHGDMLGPTVAVIGVGMNLKLSDSVQARIDQGATDIFSISGET
PDRNKLLAELLLNIARVLREFEQSGFTPFKEEWVDRHVCEGKAVTLKLPDGSGQEGLVHGVSDSGSLLLQTSLGLRSFSG
GEISLRRTA
>Mature_329_residues
MNPFTFSILRVLSDNEFHSGQAIAEALGVSRASVSNALRDADEAGLTIHKIKGRGYRLLDQVQWLERNAILEHLGHQADK
FNLEILDTIDSTNSLLLHEADNRLSLRDGLIHVVAAELQTKGRGRRGRQWHSGLGVGLAFSVLWRFQQSASFLSGLSLAT
GVAIVRALESSGIQGAVLKWPNDVMFNFCKLAGILIELHGDMLGPTVAVIGVGMNLKLSDSVQARIDQGATDIFSISGET
PDRNKLLAELLLNIARVLREFEQSGFTPFKEEWVDRHVCEGKAVTLKLPDGSGQEGLVHGVSDSGSLLLQTSLGLRSFSG
GEISLRRTA

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=325, Percent_Identity=34.1538461538462, Blast_Score=173, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR013196
- InterPro:   IPR008988
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB; PF08279 HTH_11 [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 35756; Mature: 35756

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPFTFSILRVLSDNEFHSGQAIAEALGVSRASVSNALRDADEAGLTIHKIKGRGYRLLD
CCCHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHH
QVQWLERNAILEHLGHQADKFNLEILDTIDSTNSLLLHEADNRLSLRDGLIHVVAAELQT
HHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHH
KGRGRRGRQWHSGLGVGLAFSVLWRFQQSASFLSGLSLATGVAIVRALESSGIQGAVLKW
CCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
PNDVMFNFCKLAGILIELHGDMLGPTVAVIGVGMNLKLSDSVQARIDQGATDIFSISGET
CHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEECCHHHHHHCCCCCEEEEECCCC
PDRNKLLAELLLNIARVLREFEQSGFTPFKEEWVDRHVCEGKAVTLKLPDGSGQEGLVHG
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEE
VSDSGSLLLQTSLGLRSFSGGEISLRRTA
CCCCCCEEEEECCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MNPFTFSILRVLSDNEFHSGQAIAEALGVSRASVSNALRDADEAGLTIHKIKGRGYRLLD
CCCHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHH
QVQWLERNAILEHLGHQADKFNLEILDTIDSTNSLLLHEADNRLSLRDGLIHVVAAELQT
HHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHH
KGRGRRGRQWHSGLGVGLAFSVLWRFQQSASFLSGLSLATGVAIVRALESSGIQGAVLKW
CCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
PNDVMFNFCKLAGILIELHGDMLGPTVAVIGVGMNLKLSDSVQARIDQGATDIFSISGET
CHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEECCHHHHHHCCCCCEEEEECCCC
PDRNKLLAELLLNIARVLREFEQSGFTPFKEEWVDRHVCEGKAVTLKLPDGSGQEGLVHG
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEE
VSDSGSLLLQTSLGLRSFSGGEISLRRTA
CCCCCCEEEEECCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3899863; 3536662; 8265357; 9278503; 1311302; 1328157; 1409631; 11353844 [H]