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The map label for this gene is mrcA [H]

Identifier: 82703198

GI number: 82703198

Start: 2369142

End: 2371475

Strand: Direct

Name: mrcA [H]

Synonym: Nmul_A2078

Alternate gene names: 82703198

Gene position: 2369142-2371475 (Clockwise)

Preceding gene: 82703197

Following gene: 162139861

Centisome position: 74.4

GC content: 55.18

Gene sequence:

>2334_bases
ATGCTAGCGCGTTGGCGTTGGTGGGCTTACCCTTTGGCCGCGCTTTTTGCATTGGGTGTCATCGGGGCTCTGGTTTTCGG
TTTCGCAGCATTGGTGACCATCCGTGCCTTACCCTCGCTCGAGTCTTTGACAGACTATCGCCCCAAGGTACCGTTACGCG
TATATAGCGCAGAGGGCATCCTGATCGGCGAGTTTGGAGAAGAACGGCGGCAAGTGGTCAAAATCGATTCCGTTCCGCTC
CGTCTGAAACAGGCAATCCTGGCTGCCGAGGATGACCGGTTTTATCAGCATGGGGGAGTAGACTACACTGGCATACTGCG
TGCTGCTTATTCCAATTTCACCTCCGGCGGGCTGCGCCAGGGGGCCAGCACCATTACGATGCAGGTGGCAAGAAATTTTT
TCCTGACGAAGGAAAAAACACTGAACCGTAAATTCAGCGAAGCCTTGCTTGCATTCAAGATCGAACACAGTTTAAGCAAG
GACCAGATTCTCGAACTCTACTTCAACCAGATTTATCTCGGTCAGCGTTCGTACGGTTTTGCCGCTGCCGCCCAGGTTTA
CTTCGGTAAAAGCCTGAACAGGATCAATCTGGCGGAAGCCGCAATGCTTGCGGGACTCCCCAAAGCGCCTTCGCGCTTCA
ATCCGGTCGTCAATCCGCAACGCGCGAGGGCGCGGCAAGTATATGTGCTCCGGCGTATGCGCGACTTGGGCTATATCCCG
AACGAGGAGTTCGAACAAGCCGAAAAACAACCGCTGCAAATCATCAGGCGGGAATCACAGGAATACGCTCTGCGGGCGGA
TTACGTCGCGGAAATGGCGCGCCAGGCCATGTATCAGCGCTACCAAGAGGATGCATACACGAGAGGTTACAAGGTCTATA
CCACTATCCGTTTTCTCGACCAGGAAGCCGCCTATAATGCAGTACGCCAGGGCGTGCTGGACTATGACTATCGGCACGGT
TATCGTGGTCCAGAAGCAGTCATAAAGCTGTCGGCAGGGGACTTGAAGCGGGAAGAAATACTCGATGAAGCGTTGCAGGA
AGTGACTGACAGCGATGGCATTCTTGCCGCAGTCGCGCTGGAGGCAAGCCCGAAGCGGGTCAAGGCGTACCGCAAAGGCG
GCGAAATCATCGAAATCAAGGGCGACGGACTCAAATTCGCGGAGAAATTCCTGACTGCCAAACCTGCTGCCAATCAGCAG
GTCATTCGACCCGGCTCTCTGATTCGTGTCCACAAAAACGAAAAGGGCACCTGGGAGATTACGCAACTGCCGCAGGTGGA
AGCCTCGCTGGTTGCCATTAATCCCCACGACGGGGCGATCCGCGCCATGGTGGGAGGCTTTGATTTCAACCGCAACCAGT
TCAACCACGTTACCCAGGCATGGCGCCAGCCTGGCTCCAGCTTCAAGCCATTCATTTATTCCGCTTCTCTGGAAAAAGGC
TTCACCCCGGCGACTGTCATCAATGATGCCCCGCTTTCGTTTACGGCCGAAGAGACCGGGAGCGATCAATGGGATCCCCG
AAACTTCGGGGACAGTTATGATGGGCCGTTGCGCATGCGTGAAGCGCTGGCAAGATCAAAGAATCTGGTTTCCATCCGCA
TTCTCCAGGCAATCGATGTGCAGTACGCGCAGGATTACGTGAGACGCTTCGGTTTTGATCCCAAACAGCACCCCGCCTAC
CTCGCGATGGCGCTTGGCGCCGGTTCAGTCACTCCGATGCAGATGGCGGTAGGTTACGCTGCATTCGCCAATGGCGGATT
TCTGATATCCCCCTATCTCATACAACGCGTAGAGGATGTCAACGGCAATGTCGTGGAGCAATCGAAGCCCGCGCTGGCGG
GTGAAAACGCCAAGCAGATTATCGATCCACGCAATGCCTTTCTCATGTCCAGCATGATGCGGGATGTGGTGCAACGCGGA
ACCGCAACCAGAGCGAAACAGTTGGGCCGTAACGATCTGGCGGGTAAAACCGGCACTACCAGTAATTATGTGGACGCCTG
GTTCTGCGGATATCAGCCCGATCTGGTCGCCATCGCCTGGATCGGTTTCGACAATCCGAGATCGTTGGGAGACAATGAAA
CCGGCGGTCGGGCGGCACTCCCCATCTGGATGAATTATATGGACAAGGCGTTGAAAGGCGTACCAGTCGCCTCCTATTCC
GCTCCAAGCGGGATCACAACTGCCAGGATTAATCCGGAAACCGGCCTGCGAGGGAGCGGCGCGACGCTGGAGGAATACTT
CATGGAAGAGCAGCTTCCTCCTGAAGAAGAGGATCCGATAGATATCACGATCAGATCGGTGGAGGACATCATAAACGATT
TTCTGTCGAACTAG

Upstream 100 bases:

>100_bases
AATGCTCCCCGAGCGATTGCTGTGTCTTATATGAAACGTAGTATTATTTTTATAGAACGTCCATGCTTGTCTTAATCTTC
CTCTGCAAGAACCTACTCAC

Downstream 100 bases:

>100_bases
AAAGGGGGCGATTCCCGTTTCCTGCCTGTTCGCCCTGTTTCAGTCGTCCTCCACCAGTCTTATTTCGGCTCAGCGCTGTC
TCAGCCATTCAGCGACTTCG

Product: penicillin-binding protein 1A

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 777; Mature: 777

Protein sequence:

>777_residues
MLARWRWWAYPLAALFALGVIGALVFGFAALVTIRALPSLESLTDYRPKVPLRVYSAEGILIGEFGEERRQVVKIDSVPL
RLKQAILAAEDDRFYQHGGVDYTGILRAAYSNFTSGGLRQGASTITMQVARNFFLTKEKTLNRKFSEALLAFKIEHSLSK
DQILELYFNQIYLGQRSYGFAAAAQVYFGKSLNRINLAEAAMLAGLPKAPSRFNPVVNPQRARARQVYVLRRMRDLGYIP
NEEFEQAEKQPLQIIRRESQEYALRADYVAEMARQAMYQRYQEDAYTRGYKVYTTIRFLDQEAAYNAVRQGVLDYDYRHG
YRGPEAVIKLSAGDLKREEILDEALQEVTDSDGILAAVALEASPKRVKAYRKGGEIIEIKGDGLKFAEKFLTAKPAANQQ
VIRPGSLIRVHKNEKGTWEITQLPQVEASLVAINPHDGAIRAMVGGFDFNRNQFNHVTQAWRQPGSSFKPFIYSASLEKG
FTPATVINDAPLSFTAEETGSDQWDPRNFGDSYDGPLRMREALARSKNLVSIRILQAIDVQYAQDYVRRFGFDPKQHPAY
LAMALGAGSVTPMQMAVGYAAFANGGFLISPYLIQRVEDVNGNVVEQSKPALAGENAKQIIDPRNAFLMSSMMRDVVQRG
TATRAKQLGRNDLAGKTGTTSNYVDAWFCGYQPDLVAIAWIGFDNPRSLGDNETGGRAALPIWMNYMDKALKGVPVASYS
APSGITTARINPETGLRGSGATLEEYFMEEQLPPEEEDPIDITIRSVEDIINDFLSN

Sequences:

>Translated_777_residues
MLARWRWWAYPLAALFALGVIGALVFGFAALVTIRALPSLESLTDYRPKVPLRVYSAEGILIGEFGEERRQVVKIDSVPL
RLKQAILAAEDDRFYQHGGVDYTGILRAAYSNFTSGGLRQGASTITMQVARNFFLTKEKTLNRKFSEALLAFKIEHSLSK
DQILELYFNQIYLGQRSYGFAAAAQVYFGKSLNRINLAEAAMLAGLPKAPSRFNPVVNPQRARARQVYVLRRMRDLGYIP
NEEFEQAEKQPLQIIRRESQEYALRADYVAEMARQAMYQRYQEDAYTRGYKVYTTIRFLDQEAAYNAVRQGVLDYDYRHG
YRGPEAVIKLSAGDLKREEILDEALQEVTDSDGILAAVALEASPKRVKAYRKGGEIIEIKGDGLKFAEKFLTAKPAANQQ
VIRPGSLIRVHKNEKGTWEITQLPQVEASLVAINPHDGAIRAMVGGFDFNRNQFNHVTQAWRQPGSSFKPFIYSASLEKG
FTPATVINDAPLSFTAEETGSDQWDPRNFGDSYDGPLRMREALARSKNLVSIRILQAIDVQYAQDYVRRFGFDPKQHPAY
LAMALGAGSVTPMQMAVGYAAFANGGFLISPYLIQRVEDVNGNVVEQSKPALAGENAKQIIDPRNAFLMSSMMRDVVQRG
TATRAKQLGRNDLAGKTGTTSNYVDAWFCGYQPDLVAIAWIGFDNPRSLGDNETGGRAALPIWMNYMDKALKGVPVASYS
APSGITTARINPETGLRGSGATLEEYFMEEQLPPEEEDPIDITIRSVEDIINDFLSN
>Mature_777_residues
MLARWRWWAYPLAALFALGVIGALVFGFAALVTIRALPSLESLTDYRPKVPLRVYSAEGILIGEFGEERRQVVKIDSVPL
RLKQAILAAEDDRFYQHGGVDYTGILRAAYSNFTSGGLRQGASTITMQVARNFFLTKEKTLNRKFSEALLAFKIEHSLSK
DQILELYFNQIYLGQRSYGFAAAAQVYFGKSLNRINLAEAAMLAGLPKAPSRFNPVVNPQRARARQVYVLRRMRDLGYIP
NEEFEQAEKQPLQIIRRESQEYALRADYVAEMARQAMYQRYQEDAYTRGYKVYTTIRFLDQEAAYNAVRQGVLDYDYRHG
YRGPEAVIKLSAGDLKREEILDEALQEVTDSDGILAAVALEASPKRVKAYRKGGEIIEIKGDGLKFAEKFLTAKPAANQQ
VIRPGSLIRVHKNEKGTWEITQLPQVEASLVAINPHDGAIRAMVGGFDFNRNQFNHVTQAWRQPGSSFKPFIYSASLEKG
FTPATVINDAPLSFTAEETGSDQWDPRNFGDSYDGPLRMREALARSKNLVSIRILQAIDVQYAQDYVRRFGFDPKQHPAY
LAMALGAGSVTPMQMAVGYAAFANGGFLISPYLIQRVEDVNGNVVEQSKPALAGENAKQIIDPRNAFLMSSMMRDVVQRG
TATRAKQLGRNDLAGKTGTTSNYVDAWFCGYQPDLVAIAWIGFDNPRSLGDNETGGRAALPIWMNYMDKALKGVPVASYS
APSGITTARINPETGLRGSGATLEEYFMEEQLPPEEEDPIDITIRSVEDIINDFLSN

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=804, Percent_Identity=41.044776119403, Blast_Score=574, Evalue=1e-165,
Organism=Escherichia coli, GI1786343, Length=272, Percent_Identity=32.3529411764706, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1788867, Length=327, Percent_Identity=31.1926605504587, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI1789601, Length=168, Percent_Identity=37.5, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 86744; Mature: 86744

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLARWRWWAYPLAALFALGVIGALVFGFAALVTIRALPSLESLTDYRPKVPLRVYSAEGI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEECCCE
LIGEFGEERRQVVKIDSVPLRLKQAILAAEDDRFYQHGGVDYTGILRAAYSNFTSGGLRQ
EEECCCCCCHHEEEECCCCHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHCCCCCHHHC
GASTITMQVARNFFLTKEKTLNRKFSEALLAFKIEHSLSKDQILELYFNQIYLGQRSYGF
CHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEECCCCCCH
AAAAQVYFGKSLNRINLAEAAMLAGLPKAPSRFNPVVNPQRARARQVYVLRRMRDLGYIP
HHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
NEEFEQAEKQPLQIIRRESQEYALRADYVAEMARQAMYQRYQEDAYTRGYKVYTTIRFLD
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEHHH
QEAAYNAVRQGVLDYDYRHGYRGPEAVIKLSAGDLKREEILDEALQEVTDSDGILAAVAL
HHHHHHHHHHHHHCCHHHCCCCCHHHEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEE
EASPKRVKAYRKGGEIIEIKGDGLKFAEKFLTAKPAANQQVIRPGSLIRVHKNEKGTWEI
CCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCCCCCCEECCCCEEEEECCCCCCEEE
TQLPQVEASLVAINPHDGAIRAMVGGFDFNRNQFNHVTQAWRQPGSSFKPFIYSASLEKG
EECCCCCEEEEEECCCCCCEEHEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCHHCC
FTPATVINDAPLSFTAEETGSDQWDPRNFGDSYDGPLRMREALARSKNLVSIRILQAIDV
CCCCEEECCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEHHHHHHH
QYAQDYVRRFGFDPKQHPAYLAMALGAGSVTPMQMAVGYAAFANGGFLISPYLIQRVEDV
HHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEECHHHHHHHHHC
NGNVVEQSKPALAGENAKQIIDPRNAFLMSSMMRDVVQRGTATRAKQLGRNDLAGKTGTT
CCCEECCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCC
SNYVDAWFCGYQPDLVAIAWIGFDNPRSLGDNETGGRAALPIWMNYMDKALKGVPVASYS
CCCCEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCC
APSGITTARINPETGLRGSGATLEEYFMEEQLPPEEEDPIDITIRSVEDIINDFLSN
CCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHCC
>Mature Secondary Structure
MLARWRWWAYPLAALFALGVIGALVFGFAALVTIRALPSLESLTDYRPKVPLRVYSAEGI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEECCCE
LIGEFGEERRQVVKIDSVPLRLKQAILAAEDDRFYQHGGVDYTGILRAAYSNFTSGGLRQ
EEECCCCCCHHEEEECCCCHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHCCCCCHHHC
GASTITMQVARNFFLTKEKTLNRKFSEALLAFKIEHSLSKDQILELYFNQIYLGQRSYGF
CHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEECCCCCCH
AAAAQVYFGKSLNRINLAEAAMLAGLPKAPSRFNPVVNPQRARARQVYVLRRMRDLGYIP
HHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
NEEFEQAEKQPLQIIRRESQEYALRADYVAEMARQAMYQRYQEDAYTRGYKVYTTIRFLD
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEHHH
QEAAYNAVRQGVLDYDYRHGYRGPEAVIKLSAGDLKREEILDEALQEVTDSDGILAAVAL
HHHHHHHHHHHHHCCHHHCCCCCHHHEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEE
EASPKRVKAYRKGGEIIEIKGDGLKFAEKFLTAKPAANQQVIRPGSLIRVHKNEKGTWEI
CCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCCCCCCEECCCCEEEEECCCCCCEEE
TQLPQVEASLVAINPHDGAIRAMVGGFDFNRNQFNHVTQAWRQPGSSFKPFIYSASLEKG
EECCCCCEEEEEECCCCCCEEHEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCHHCC
FTPATVINDAPLSFTAEETGSDQWDPRNFGDSYDGPLRMREALARSKNLVSIRILQAIDV
CCCCEEECCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEHHHHHHH
QYAQDYVRRFGFDPKQHPAYLAMALGAGSVTPMQMAVGYAAFANGGFLISPYLIQRVEDV
HHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEECHHHHHHHHHC
NGNVVEQSKPALAGENAKQIIDPRNAFLMSSMMRDVVQRGTATRAKQLGRNDLAGKTGTT
CCCEECCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCC
SNYVDAWFCGYQPDLVAIAWIGFDNPRSLGDNETGGRAALPIWMNYMDKALKGVPVASYS
CCCCEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCC
APSGITTARINPETGLRGSGATLEEYFMEEQLPPEEEDPIDITIRSVEDIINDFLSN
CCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA