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The map label for this gene is sbp [H]

Identifier: 82703153

GI number: 82703153

Start: 2327271

End: 2328368

Strand: Direct

Name: sbp [H]

Synonym: Nmul_A2032

Alternate gene names: 82703153

Gene position: 2327271-2328368 (Clockwise)

Preceding gene: 82703151

Following gene: 82703154

Centisome position: 73.09

GC content: 52.55

Gene sequence:

>1098_bases
ATGACACCCGATAGTTCCCGTTTTGCTGTAGGTATATTTATAGTGTTGGTGAGTCTCGGCGGCGCCTACGCTACTCTGAA
TAATTTTTCCGATCGTCCTGCCGGCTCCGCTGAGCGGTTACACGAGGAATTGAACGTCGGTTTTGCCTCTCACTGGAGAG
CTCGGACGGGCGTGAACATCAAGGTCGATCAGGCCCGGAGCAGGTCGGGGAAGCCCGTACATATTACGCTCGATGGGCTT
GATATTCCCGCCCTTGCGCTGTCCTACGATGTGGACAAGCTGCATGATAAGGAAAGATTTATTGCACCTGACTTCCGTCA
GCTTTTAGCGCAGGATTTTCGGACTGGCTCTTATCCTTCCCCCTATACCTCGACCATCGTATTCCTGGTAAGGAAAGGCA
ATCCAAAGAAACTCAAGGACTGGGGCGATCTGGTACGCTCGGATATAAAAGTAGTTACACCCAATCCAAGGCATTCTGAA
AGCGGCCGCTGGAATTATCTTGCGGCGTGGGGATACGCCGTGAGGCGATCAGGCGGCAGCGAACAAGCTGCGCGTGAATT
TGTCAGTCAGTTGTTTGCCAATGTCCAGACAGTGGATTACGAGGGTAAAAAGCCGGGAAACTTGGGTGCCGCCTTTGTCT
TTCGCAACATCGGCGATGTACTCCTCACCTGGGAAAATGAAGCGTACCTGATCGTTCAGAACAGTGGAGCCGATAAGTTT
GAAGTCATCACCCCGTCCATATCAATAGTGGCTGAACCCGCTATAAGTGTGGTGGACGCAGCTGCACGTGGGAAAAGCAC
ACGCCGTGTAGCGGCTTCATACATCGAATACTTATATACACCCCAGGCGCAGCATATCGCCGCCAAGCACTATTACCGTC
CCCGCGATCCGGCCATTACCACGAAGTACGTGGACAGGTTTCCGCGCCTTGAGTTGTTTACAGTTGACGAGGTTTCCAGT
GGCTGGCAGAAAGCCCAGAAAATACATTTTGCCAGGGGCGGTGTTTTTGACCAGATCACCGGTGATGTTCCGAATTCTGT
CGCCGTGAGGGGCGCTATCGATAGGGACCATATTCAAGCCGGCAATGCTAAAGGCTGA

Upstream 100 bases:

>100_bases
CCAGGTAACACCAATTCCTGTGTCTGTTCTGATGGAGGAAGCGGTGAACAAGCACCAATTTATGCTCGATCCATTTTGTG
TTACTTGGAGGAAATTAAAA

Downstream 100 bases:

>100_bases
ACAGCAAATGACAAGGAAAAACGGGAAGCTGGCTGACAAAGCCATCCTGGACTTTCCCTTGTACAAAAATTCCCATCCAA
CCACTACAGGCCCTTTGCAG

Product: thiosulfate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MTPDSSRFAVGIFIVLVSLGGAYATLNNFSDRPAGSAERLHEELNVGFASHWRARTGVNIKVDQARSRSGKPVHITLDGL
DIPALALSYDVDKLHDKERFIAPDFRQLLAQDFRTGSYPSPYTSTIVFLVRKGNPKKLKDWGDLVRSDIKVVTPNPRHSE
SGRWNYLAAWGYAVRRSGGSEQAAREFVSQLFANVQTVDYEGKKPGNLGAAFVFRNIGDVLLTWENEAYLIVQNSGADKF
EVITPSISIVAEPAISVVDAAARGKSTRRVAASYIEYLYTPQAQHIAAKHYYRPRDPAITTKYVDRFPRLELFTVDEVSS
GWQKAQKIHFARGGVFDQITGDVPNSVAVRGAIDRDHIQAGNAKG

Sequences:

>Translated_365_residues
MTPDSSRFAVGIFIVLVSLGGAYATLNNFSDRPAGSAERLHEELNVGFASHWRARTGVNIKVDQARSRSGKPVHITLDGL
DIPALALSYDVDKLHDKERFIAPDFRQLLAQDFRTGSYPSPYTSTIVFLVRKGNPKKLKDWGDLVRSDIKVVTPNPRHSE
SGRWNYLAAWGYAVRRSGGSEQAAREFVSQLFANVQTVDYEGKKPGNLGAAFVFRNIGDVLLTWENEAYLIVQNSGADKF
EVITPSISIVAEPAISVVDAAARGKSTRRVAASYIEYLYTPQAQHIAAKHYYRPRDPAITTKYVDRFPRLELFTVDEVSS
GWQKAQKIHFARGGVFDQITGDVPNSVAVRGAIDRDHIQAGNAKG
>Mature_364_residues
TPDSSRFAVGIFIVLVSLGGAYATLNNFSDRPAGSAERLHEELNVGFASHWRARTGVNIKVDQARSRSGKPVHITLDGLD
IPALALSYDVDKLHDKERFIAPDFRQLLAQDFRTGSYPSPYTSTIVFLVRKGNPKKLKDWGDLVRSDIKVVTPNPRHSES
GRWNYLAAWGYAVRRSGGSEQAAREFVSQLFANVQTVDYEGKKPGNLGAAFVFRNIGDVLLTWENEAYLIVQNSGADKFE
VITPSISIVAEPAISVVDAAARGKSTRRVAASYIEYLYTPQAQHIAAKHYYRPRDPAITTKYVDRFPRLELFTVDEVSSG
WQKAQKIHFARGGVFDQITGDVPNSVAVRGAIDRDHIQAGNAKG

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=339, Percent_Identity=45.4277286135693, Blast_Score=300, Evalue=7e-83,
Organism=Escherichia coli, GI1788765, Length=308, Percent_Identity=37.012987012987, Blast_Score=207, Evalue=6e-55,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40382; Mature: 40251

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPDSSRFAVGIFIVLVSLGGAYATLNNFSDRPAGSAERLHEELNVGFASHWRARTGVNI
CCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCEE
KVDQARSRSGKPVHITLDGLDIPALALSYDVDKLHDKERFIAPDFRQLLAQDFRTGSYPS
EEECCCCCCCCEEEEEECCCCCCHHEEECCHHHHCCCCCCCCHHHHHHHHHHHCCCCCCC
PYTSTIVFLVRKGNPKKLKDWGDLVRSDIKVVTPNPRHSESGRWNYLAAWGYAVRRSGGS
CCCEEEEEEEECCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEEEHHHHHHCCCCC
EQAAREFVSQLFANVQTVDYEGKKPGNLGAAFVFRNIGDVLLTWENEAYLIVQNSGADKF
HHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHCCCCEEEEECCCEEEEEECCCCCEE
EVITPSISIVAEPAISVVDAAARGKSTRRVAASYIEYLYTPQAQHIAAKHYYRPRDPAIT
EEECCCCEEEECHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHH
TKYVDRFPRLELFTVDEVSSGWQKAQKIHFARGGVFDQITGDVPNSVAVRGAIDRDHIQA
HHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCEEEEECCCHHHHCC
GNAKG
CCCCC
>Mature Secondary Structure 
TPDSSRFAVGIFIVLVSLGGAYATLNNFSDRPAGSAERLHEELNVGFASHWRARTGVNI
CCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCEE
KVDQARSRSGKPVHITLDGLDIPALALSYDVDKLHDKERFIAPDFRQLLAQDFRTGSYPS
EEECCCCCCCCEEEEEECCCCCCHHEEECCHHHHCCCCCCCCHHHHHHHHHHHCCCCCCC
PYTSTIVFLVRKGNPKKLKDWGDLVRSDIKVVTPNPRHSESGRWNYLAAWGYAVRRSGGS
CCCEEEEEEEECCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEEEHHHHHHCCCCC
EQAAREFVSQLFANVQTVDYEGKKPGNLGAAFVFRNIGDVLLTWENEAYLIVQNSGADKF
HHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHCCCCEEEEECCCEEEEEECCCCCEE
EVITPSISIVAEPAISVVDAAARGKSTRRVAASYIEYLYTPQAQHIAAKHYYRPRDPAIT
EEECCCCEEEECHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHH
TKYVDRFPRLELFTVDEVSSGWQKAQKIHFARGGVFDQITGDVPNSVAVRGAIDRDHIQA
HHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCEEEEECCCHHHHCC
GNAKG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]