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The map label for this gene is pinE [H]

Identifier: 82703144

GI number: 82703144

Start: 2319475

End: 2319711

Strand: Reverse

Name: pinE [H]

Synonym: Nmul_A2023

Alternate gene names: 82703144

Gene position: 2319711-2319475 (Counterclockwise)

Preceding gene: 82703145

Following gene: 82703143

Centisome position: 72.85

GC content: 58.23

Gene sequence:

>237_bases
ATGGCCAGCCTCGCCGAGATGGAGCGGGAACTGACGATAGAGCGCACGCATACCGGCCTGGAAGTCGCTCGCCAGCTAGG
GCGCAAGGGCGGCCGAAAACGCCAGATGACCGACAGCAAGATCGCTTCTGCAAAAAAACTGTTAACTAATGGCGTACCGC
CACGGGATGTGGCCAGGAACCTCGGCGTATCCATTCCAACGCTTTATCGCTGGATTCCGGCTTCCGAACAGCCTTAA

Upstream 100 bases:

>100_bases
CGGATACATTGGCGAGCTCCATAAGCAAGGCGTCCAGTTCAAGAGCCTCACCGACGCTATCGATACCGGTACCCCATAAG
GCCGTTTCTTCTTCCATGTC

Downstream 100 bases:

>100_bases
TGTACTTTGCGATCCGTTTTCTGAGACGACCCCTAAGTATGATTCCATACGACCATTTTAGGGTTGGAATTTGCTGCGGG
AGAGCTTGCCGCAATGGAAC

Product: resolvase helix-turn-helix region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 78; Mature: 77

Protein sequence:

>78_residues
MASLAEMERELTIERTHTGLEVARQLGRKGGRKRQMTDSKIASAKKLLTNGVPPRDVARNLGVSIPTLYRWIPASEQP

Sequences:

>Translated_78_residues
MASLAEMERELTIERTHTGLEVARQLGRKGGRKRQMTDSKIASAKKLLTNGVPPRDVARNLGVSIPTLYRWIPASEQP
>Mature_77_residues
ASLAEMERELTIERTHTGLEVARQLGRKGGRKRQMTDSKIASAKKLLTNGVPPRDVARNLGVSIPTLYRWIPASEQP

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=77, Percent_Identity=46.7532467532467, Blast_Score=69, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 8684; Mature: 8553

Theoretical pI: Translated: 11.22; Mature: 11.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLAEMERELTIERTHTGLEVARQLGRKGGRKRQMTDSKIASAKKLLTNGVPPRDVARN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
LGVSIPTLYRWIPASEQP
CCCCCHHHHHHCCCCCCC
>Mature Secondary Structure 
ASLAEMERELTIERTHTGLEVARQLGRKGGRKRQMTDSKIASAKKLLTNGVPPRDVARN
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
LGVSIPTLYRWIPASEQP
CCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]