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The map label for this gene is aniA [H]

Identifier: 82703119

GI number: 82703119

Start: 2295049

End: 2296404

Strand: Direct

Name: aniA [H]

Synonym: Nmul_A1998

Alternate gene names: 82703119

Gene position: 2295049-2296404 (Clockwise)

Preceding gene: 82703106

Following gene: 82703121

Centisome position: 72.08

GC content: 54.94

Gene sequence:

>1356_bases
ATGGGAAGGACCTTTTTACTGCTTACAGCCGTTTGCGCAACGTTCGGTTATTTATCCCCGGTCGCTGCCGAACAAGGCAG
CAACAGCGGTGTCACCTACCGACCCGACGTTACCTTTACCCTTCGTACGGATATCGGCGAGGGTAAACTGGTATTTGTCG
GTGAATCAGGTACGATCGCGGGCAAAATCAACCCTGACCTGGAGGTAAACCCGGGCGCAGTGGTTCAGATCAATCTGGTC
AACGGCGATGGTGCCATGCATGACATCGCCATTCCTGAGCTGGGCGCCAAATCGGATAATATCACAGCGAAAGGCGCTGC
CACTTCGATGGTATTTCGTGCAGTCCGTACCGGCACCTTTGAGTACCTCTGCACACTGCCCGGGCACAAGGCCGCCGGCA
TGTTCGGCCGTCTCATTGTCGGCAAGCCGCCGGAGGAAGCGAAAGGATATGTAACCAACGTGGCCCAGGACCCGCGCGCC
GTGGGAGAGCCGGTCGGAACGCGGGCTTCAAGGCATTTGACACTCAATCTCGAAGCAACCGAAATCGAAGGGCAACTTTC
GGACAAGAAGCTCTATAAATACTGGACCTTCAATAACAGGGTGCCGGGACCCCTCCTGCGCGTGAAGGTAGGGGATACCA
TCACTATCAATCTGCATAATAGCGCAAGCAGTACCAACATCCATTCGATAGATTTCCATGCCGTAACAGGACCGGGTGGA
GGTGCCGCGGTCACGCAGGCGGCACCGGGCGAGACAAAGAGCTTCACCTTCAAGTCGCTTCATCCTGGACTGTTCGTTTA
CCATTGTGCAACTCCCATGGTTGCGCATCATATTGCGAACGGCATGTATGGCATGGTTCTGGTCGAGCCCGAGGGAGGTC
TGCCCATGGCTGACAGGGAATTCTATGTAATGCAGGGTGAGCTCTACACCACCCACTCCCACAATGTGCGAGGTCTGCAG
GAATTTTCGCTGGAAAATCTGCTCGCGGAGAATCCCCAGCACCTAGTGTTCAATGGCACCGTGGATGCGCTTACGAAAAT
GTACACCATGGAAGCGAACACGGGCGATAACGTGCGCATCTATTTTGGTGTGGGCGGTCCCAACCTCACCTCCAGCTTCC
ACATCATCGGGGAAGTCTTCGATAAGGTGTACGACCAGGCTTCATTGACCAGTCCGCCATTGACAGATGTGCAAACGACG
CTCGTTCCGCCAGGCGGTGCCACCATTGTGGAATTCAAGGTCGATTATCCCGGCCGCTATATTCTGGTTGATCACGCGCT
GTCGCGTATGGAGAAAGGATTAGCAGGTTATCTCACGGTGCGTGGGAAGGCAAACGCGGAAATATTCAAGCCGTAA

Upstream 100 bases:

>100_bases
AAATATCGGGGAGCATTGCAGGAAAAACATCCTGCTGCCCATACGAGCTTAGCGGTATTTGTATCCGCAATCTTTGTATC
CGTTTATCTGGGAGAAAAGC

Downstream 100 bases:

>100_bases
AGAATTGAAAAAAATCAGAGGCGATTTCCCCGCAAACGATCGCCTCTGATCCTTTATATCATTGGTGTGGCTGCTTATAC
GGTTTCTTGTTCATTACCTC

Product: nitrite reductase (NO-forming)

Products: NA

Alternate protein names: Major outer membrane protein Pan 1 [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MGRTFLLLTAVCATFGYLSPVAAEQGSNSGVTYRPDVTFTLRTDIGEGKLVFVGESGTIAGKINPDLEVNPGAVVQINLV
NGDGAMHDIAIPELGAKSDNITAKGAATSMVFRAVRTGTFEYLCTLPGHKAAGMFGRLIVGKPPEEAKGYVTNVAQDPRA
VGEPVGTRASRHLTLNLEATEIEGQLSDKKLYKYWTFNNRVPGPLLRVKVGDTITINLHNSASSTNIHSIDFHAVTGPGG
GAAVTQAAPGETKSFTFKSLHPGLFVYHCATPMVAHHIANGMYGMVLVEPEGGLPMADREFYVMQGELYTTHSHNVRGLQ
EFSLENLLAENPQHLVFNGTVDALTKMYTMEANTGDNVRIYFGVGGPNLTSSFHIIGEVFDKVYDQASLTSPPLTDVQTT
LVPPGGATIVEFKVDYPGRYILVDHALSRMEKGLAGYLTVRGKANAEIFKP

Sequences:

>Translated_451_residues
MGRTFLLLTAVCATFGYLSPVAAEQGSNSGVTYRPDVTFTLRTDIGEGKLVFVGESGTIAGKINPDLEVNPGAVVQINLV
NGDGAMHDIAIPELGAKSDNITAKGAATSMVFRAVRTGTFEYLCTLPGHKAAGMFGRLIVGKPPEEAKGYVTNVAQDPRA
VGEPVGTRASRHLTLNLEATEIEGQLSDKKLYKYWTFNNRVPGPLLRVKVGDTITINLHNSASSTNIHSIDFHAVTGPGG
GAAVTQAAPGETKSFTFKSLHPGLFVYHCATPMVAHHIANGMYGMVLVEPEGGLPMADREFYVMQGELYTTHSHNVRGLQ
EFSLENLLAENPQHLVFNGTVDALTKMYTMEANTGDNVRIYFGVGGPNLTSSFHIIGEVFDKVYDQASLTSPPLTDVQTT
LVPPGGATIVEFKVDYPGRYILVDHALSRMEKGLAGYLTVRGKANAEIFKP
>Mature_450_residues
GRTFLLLTAVCATFGYLSPVAAEQGSNSGVTYRPDVTFTLRTDIGEGKLVFVGESGTIAGKINPDLEVNPGAVVQINLVN
GDGAMHDIAIPELGAKSDNITAKGAATSMVFRAVRTGTFEYLCTLPGHKAAGMFGRLIVGKPPEEAKGYVTNVAQDPRAV
GEPVGTRASRHLTLNLEATEIEGQLSDKKLYKYWTFNNRVPGPLLRVKVGDTITINLHNSASSTNIHSIDFHAVTGPGGG
AAVTQAAPGETKSFTFKSLHPGLFVYHCATPMVAHHIANGMYGMVLVEPEGGLPMADREFYVMQGELYTTHSHNVRGLQE
FSLENLLAENPQHLVFNGTVDALTKMYTMEANTGDNVRIYFGVGGPNLTSSFHIIGEVFDKVYDQASLTSPPLTDVQTTL
VPPGGATIVEFKVDYPGRYILVDHALSRMEKGLAGYLTVRGKANAEIFKP

Specific function: Catalyzes the reduction of nitrite to nitric oxide (NO), probably with azurin as electron donor. Essential for growth and survival in oxygen-depleted environments. Can also provide protection against killing by normal human sera [H]

COG id: COG2132

COG function: function code Q; Putative multicopper oxidases

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 2 plastocyanin-like domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001117
- InterPro:   IPR011707
- InterPro:   IPR008972
- InterPro:   IPR001287 [H]

Pfam domain/function: PF00394 Cu-oxidase; PF07732 Cu-oxidase_3 [H]

EC number: =1.7.2.1 [H]

Molecular weight: Translated: 48388; Mature: 48256

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00079 MULTICOPPER_OXIDASE1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRTFLLLTAVCATFGYLSPVAAEQGSNSGVTYRPDVTFTLRTDIGEGKLVFVGESGTIA
CCCEEHHHHHHHHHHHCCCCHHHCCCCCCCEEECCCEEEEEEECCCCCEEEEEECCCEEE
GKINPDLEVNPGAVVQINLVNGDGAMHDIAIPELGAKSDNITAKGAATSMVFRAVRTGTF
EECCCCCEECCCCEEEEEEECCCCCEEEEECCCCCCCCCCEEECCHHHHHHHHHHHCCCE
EYLCTLPGHKAAGMFGRLIVGKPPEEAKGYVTNVAQDPRAVGEPVGTRASRHLTLNLEAT
EEEEECCCCCCCCCCEEEEECCCCHHHCCCEEECCCCCHHHCCCCCCCCCEEEEEEEEEE
EIEGQLSDKKLYKYWTFNNRVPGPLLRVKVGDTITINLHNSASSTNIHSIDFHAVTGPGG
EECCCCCCCEEEEEEEECCCCCCCEEEEEECCEEEEEEECCCCCCEEEEEEEEEEECCCC
GAAVTQAAPGETKSFTFKSLHPGLFVYHCATPMVAHHIANGMYGMVLVEPEGGLPMADRE
CCEEECCCCCCCCEEEEECCCCCEEEEECCCHHHHHHHCCCCEEEEEECCCCCCCCCCCE
FYVMQGELYTTHSHNVRGLQEFSLENLLAENPQHLVFNGTVDALTKMYTMEANTGDNVRI
EEEEECEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCHHHHEEEEEEECCCCCCEEE
YFGVGGPNLTSSFHIIGEVFDKVYDQASLTSPPLTDVQTTLVPPGGATIVEFKVDYPGRY
EEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCE
ILVDHALSRMEKGLAGYLTVRGKANAEIFKP
EEHHHHHHHHHCCCEEEEEEECCCCCEECCC
>Mature Secondary Structure 
GRTFLLLTAVCATFGYLSPVAAEQGSNSGVTYRPDVTFTLRTDIGEGKLVFVGESGTIA
CCEEHHHHHHHHHHHCCCCHHHCCCCCCCEEECCCEEEEEEECCCCCEEEEEECCCEEE
GKINPDLEVNPGAVVQINLVNGDGAMHDIAIPELGAKSDNITAKGAATSMVFRAVRTGTF
EECCCCCEECCCCEEEEEEECCCCCEEEEECCCCCCCCCCEEECCHHHHHHHHHHHCCCE
EYLCTLPGHKAAGMFGRLIVGKPPEEAKGYVTNVAQDPRAVGEPVGTRASRHLTLNLEAT
EEEEECCCCCCCCCCEEEEECCCCHHHCCCEEECCCCCHHHCCCCCCCCCEEEEEEEEEE
EIEGQLSDKKLYKYWTFNNRVPGPLLRVKVGDTITINLHNSASSTNIHSIDFHAVTGPGG
EECCCCCCCEEEEEEEECCCCCCCEEEEEECCEEEEEEECCCCCCEEEEEEEEEEECCCC
GAAVTQAAPGETKSFTFKSLHPGLFVYHCATPMVAHHIANGMYGMVLVEPEGGLPMADRE
CCEEECCCCCCCCEEEEECCCCCEEEEECCCHHHHHHHCCCCEEEEEECCCCCCCCCCCE
FYVMQGELYTTHSHNVRGLQEFSLENLLAENPQHLVFNGTVDALTKMYTMEANTGDNVRI
EEEEECEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCHHHHEEEEEEECCCCCCEEE
YFGVGGPNLTSSFHIIGEVFDKVYDQASLTSPPLTDVQTTLVPPGGATIVEFKVDYPGRY
EEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCE
ILVDHALSRMEKGLAGYLTVRGKANAEIFKP
EEHHHHHHHHHCCCEEEEEEECCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1383156; 1398981 [H]