Click here to switch to the map view.

The map label for this gene is oprM [H]

Identifier: 82702961

GI number: 82702961

Start: 2120944

End: 2122332

Strand: Direct

Name: oprM [H]

Synonym: Nmul_A1840

Alternate gene names: 82702961

Gene position: 2120944-2122332 (Clockwise)

Preceding gene: 82702959

Following gene: 82702962

Centisome position: 66.61

GC content: 57.74

Gene sequence:

>1389_bases
ATGGCCAGAATATTTGCGATAATAATTGTTTCGATTTCCCTCGCCGGAATTGGCGGTTGCAAAACATCTCCACTGAGAAA
TCCTGGAGATGCAGGCGCAGCAATCCCAGCCAGATGGACAGCGGATAAAGGGACTCCACAACCCACCCCGGAAAACTGGG
TAGAAACTTTTGGCGACCCTAATCTGAGCGCCGTGGTTAACAGCGCCCTGGCGGATAATTATCAACTGAAGGCTGCGGCG
GCGCGGGTAAAAGCTGCGGTGGCCCAGGCGCGCATAGACGGTGCAAGCCGATGGCCGCAACTGCTCTTTGCCCCGGATTA
CCAATATGCGCAAATCCGCGAGGAGGGATTCGGCTCAGCTCGCTTCAGTGTGTTTGAAACATTGTTCAGCCTGAGCTGGG
AGCTGGATGTGTGGGGACGGATAAGCGACTTCCAGCAGGCAGCAATAAGAGAAGCGAATGCGACCGCAACCGATTTTCAA
GGGGCGCGCCTGTCTCTGGCCGCACGGGTGGCGCAGAGTTATTTTGAGCTTGCCGAAGCCAGACTGCAAGCAGCAGTGGC
GGAGCGATCGGTAAAGGATCGCAGCGTTATTGTCGACCTGGTGCGGGGGCGCTTCGCCCGGGGTTTGACGAAAGCGCTGG
ATGTGCGCCTGGCCCTCACCGACCTTGCCAACGCGGAGGCCGAGCTTACCCAGGCGCGCAACCAAGTTCAGATCGTGGCA
CGCCGACTGCAAGTGCTGCTTGGGCGCTATCCGGAAGCTGTGCTGAGGGCGACTGCGGAACTGCCTGAACTTCCACCCGA
TATGCCAGCGGGGTTGCCCTCGGAATTGCTGGAAAGACGGCCGGATCTCGTCTCTGCCTATGATCGTTTACTGGCTGCCG
ACTCCCGTCTGCAAAGCACGAGAAAATTACTGCTGCCGCGAGTTGCCCTGACTGCAGCGGGAGGAATGCGCAGCCAGGCA
CTGACCGAATTGGTGGATCCCAGGGCTCTCGCATGGAACGTGCTCATCGGGTTGGCGCAACCGGTATTCACGGGCGGGCG
CATCCGCGGCCAGATCGAACTTGACAAGGCACAGACCGAGCAGGCCCTTAATCTGTACAAGGACACGGCACTCAATGCTT
TCCGGGAAGTGGAGCAGGCGCTGGCAGCCGACGAATGGCTACGGAAGGAAGAAAAAGCCTTGAGAAGTGCGGTTGAGCAA
ACCGAAGCAAGCCGAAAACTGGCAGTCTATTCCTATCAACATGGGTATATCGAAATTCTTACCCTGCTGGATAGCTACCG
CAGCACCCTCACTGCCCAGAGTGCGCATCTTTCCGTCAGGCGCCAGCTTCTGAGCAATCGAATCAATCTTTACCTGGCAT
TAGGAGGCAGCATTTGCGAGGCGTGCTGA

Upstream 100 bases:

>100_bases
CTTATTATCATATAGCAATTTCTATTTTACAATATGGATCAACTTCCGGTTCTGAAGGATAGAGTGGATATCCTCACTTT
CCGGATCGGCTTGAACCGAA

Downstream 100 bases:

>100_bases
AACAACTGGCAAGCAAGGGCATGCTCAAAATCATCATCCCCGTTCTTGTTGTGCTGGCCGGCATCAGTATTTCGTGGGCG
ATTGCTGTTCACCGCCCGTC

Product: RND efflux system outer membrane lipoprotein NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MARIFAIIIVSISLAGIGGCKTSPLRNPGDAGAAIPARWTADKGTPQPTPENWVETFGDPNLSAVVNSALADNYQLKAAA
ARVKAAVAQARIDGASRWPQLLFAPDYQYAQIREEGFGSARFSVFETLFSLSWELDVWGRISDFQQAAIREANATATDFQ
GARLSLAARVAQSYFELAEARLQAAVAERSVKDRSVIVDLVRGRFARGLTKALDVRLALTDLANAEAELTQARNQVQIVA
RRLQVLLGRYPEAVLRATAELPELPPDMPAGLPSELLERRPDLVSAYDRLLAADSRLQSTRKLLLPRVALTAAGGMRSQA
LTELVDPRALAWNVLIGLAQPVFTGGRIRGQIELDKAQTEQALNLYKDTALNAFREVEQALAADEWLRKEEKALRSAVEQ
TEASRKLAVYSYQHGYIEILTLLDSYRSTLTAQSAHLSVRRQLLSNRINLYLALGGSICEAC

Sequences:

>Translated_462_residues
MARIFAIIIVSISLAGIGGCKTSPLRNPGDAGAAIPARWTADKGTPQPTPENWVETFGDPNLSAVVNSALADNYQLKAAA
ARVKAAVAQARIDGASRWPQLLFAPDYQYAQIREEGFGSARFSVFETLFSLSWELDVWGRISDFQQAAIREANATATDFQ
GARLSLAARVAQSYFELAEARLQAAVAERSVKDRSVIVDLVRGRFARGLTKALDVRLALTDLANAEAELTQARNQVQIVA
RRLQVLLGRYPEAVLRATAELPELPPDMPAGLPSELLERRPDLVSAYDRLLAADSRLQSTRKLLLPRVALTAAGGMRSQA
LTELVDPRALAWNVLIGLAQPVFTGGRIRGQIELDKAQTEQALNLYKDTALNAFREVEQALAADEWLRKEEKALRSAVEQ
TEASRKLAVYSYQHGYIEILTLLDSYRSTLTAQSAHLSVRRQLLSNRINLYLALGGSICEAC
>Mature_461_residues
ARIFAIIIVSISLAGIGGCKTSPLRNPGDAGAAIPARWTADKGTPQPTPENWVETFGDPNLSAVVNSALADNYQLKAAAA
RVKAAVAQARIDGASRWPQLLFAPDYQYAQIREEGFGSARFSVFETLFSLSWELDVWGRISDFQQAAIREANATATDFQG
ARLSLAARVAQSYFELAEARLQAAVAERSVKDRSVIVDLVRGRFARGLTKALDVRLALTDLANAEAELTQARNQVQIVAR
RLQVLLGRYPEAVLRATAELPELPPDMPAGLPSELLERRPDLVSAYDRLLAADSRLQSTRKLLLPRVALTAAGGMRSQAL
TELVDPRALAWNVLIGLAQPVFTGGRIRGQIELDKAQTEQALNLYKDTALNAFREVEQALAADEWLRKEEKALRSAVEQT
EASRKLAVYSYQHGYIEILTLLDSYRSTLTAQSAHLSVRRQLLSNRINLYLALGGSICEAC

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=425, Percent_Identity=29.1764705882353, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI2367348, Length=434, Percent_Identity=25.3456221198157, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50582; Mature: 50451

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIFAIIIVSISLAGIGGCKTSPLRNPGDAGAAIPARWTADKGTPQPTPENWVETFGDP
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCC
NLSAVVNSALADNYQLKAAAARVKAAVAQARIDGASRWPQLLFAPDYQYAQIREEGFGSA
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHCCCCEEECCCCHHHHHHHHCCCHH
RFSVFETLFSLSWELDVWGRISDFQQAAIREANATATDFQGARLSLAARVAQSYFELAEA
HHHHHHHHHHHCCCEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
RLQAAVAERSVKDRSVIVDLVRGRFARGLTKALDVRLALTDLANAEAELTQARNQVQIVA
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RRLQVLLGRYPEAVLRATAELPELPPDMPAGLPSELLERRPDLVSAYDRLLAADSRLQST
HHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHH
RKLLLPRVALTAAGGMRSQALTELVDPRALAWNVLIGLAQPVFTGGRIRGQIELDKAQTE
HHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHHH
QALNLYKDTALNAFREVEQALAADEWLRKEEKALRSAVEQTEASRKLAVYSYQHGYIEIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHH
TLLDSYRSTLTAQSAHLSVRRQLLSNRINLYLALGGSICEAC
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHCCC
>Mature Secondary Structure 
ARIFAIIIVSISLAGIGGCKTSPLRNPGDAGAAIPARWTADKGTPQPTPENWVETFGDP
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCC
NLSAVVNSALADNYQLKAAAARVKAAVAQARIDGASRWPQLLFAPDYQYAQIREEGFGSA
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHCCCCEEECCCCHHHHHHHHCCCHH
RFSVFETLFSLSWELDVWGRISDFQQAAIREANATATDFQGARLSLAARVAQSYFELAEA
HHHHHHHHHHHCCCEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
RLQAAVAERSVKDRSVIVDLVRGRFARGLTKALDVRLALTDLANAEAELTQARNQVQIVA
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RRLQVLLGRYPEAVLRATAELPELPPDMPAGLPSELLERRPDLVSAYDRLLAADSRLQST
HHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHH
RKLLLPRVALTAAGGMRSQALTELVDPRALAWNVLIGLAQPVFTGGRIRGQIELDKAQTE
HHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHHH
QALNLYKDTALNAFREVEQALAADEWLRKEEKALRSAVEQTEASRKLAVYSYQHGYIEIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHH
TLLDSYRSTLTAQSAHLSVRRQLLSNRINLYLALGGSICEAC
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]