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The map label for this gene is lip2 [H]

Identifier: 82702950

GI number: 82702950

Start: 2088427

End: 2089398

Strand: Direct

Name: lip2 [H]

Synonym: Nmul_A1827

Alternate gene names: 82702950

Gene position: 2088427-2089398 (Clockwise)

Preceding gene: 82702949

Following gene: 82702959

Centisome position: 65.59

GC content: 58.44

Gene sequence:

>972_bases
ATGGCACTTGATCCCGATCTTGCAGCATTTCTCGAACTTGTGGAAGCGGGCATCAGCAATGGTGTCCCTCGCTTGCACGA
GCTGCCGCCCGCGAAAGCGCGCGAACAATACGATATATCCACCCTGGCTCTGGATAGCCCAGGTATGGAGGTCGCGAGCG
TCAAGGAAATCGGCATCCCTTCCCGGGATGGAAATGAGATAACGGCAAGACTCTATGCGCCTCTGCTGGATGAGCCGTTG
AACGGCCTGGCGGCGCCCGCGCTGCTGTATTTTCATGGAGGCGGATATTGCGTGGGCAGCCTGGATTCCCACGACTCGCT
ATGCCGGACACTGGCTGCGCTGACCCCCTGCTGCGTGCTGAACGCCAGCTACCGGTTGGCGCCTGAGCATCCATTCCCCA
CGGCGGTACATGATGCGCAGGATGCCTACCGATGGCTTCTTTCCAACGGACTCGCTCATGGTATCGATCCTCAACGGATA
GCCGTGGGAGGCGATAGCGCAGGGGGCACGCTGGCCATCGGATTGACTATCGCCGCCAGGGAAATGGATTGGCCACAACC
GGTATTCCAGGCGTTGCTCTACCCGTGCACAAGCGCGTGGCAGAATACCGATTCGCACCGCCGCTTCGCAAAGGGATACC
TGCTCGAAGCTGCGACACTGCAATGGATGTTCAGCAATTACCTCACCAGCGAAAGGGACCGGACAGACTGGCGTTTTGCT
CCCCTCGAGGCAAAGGATTTGAGCAGCCTCGCTCCGGCATTCATCGCCGTGGCGGAATACGATCCGCTCGTGGATGAAGG
AATTGAATACGCCAACAGACTCAAAGATGCCGGCGTTCCCACTCAGCTCAAAATCTACGAGGGCATGACGCACGACTTTG
CGCGCCTGACGAATATCCTCAACGACGCAAGCAAGGTGCGTGAAGAAGTGGCGCAGCAATTACAGAGGGCTTTTGCTGCG
CCTCGAACCTAG

Upstream 100 bases:

>100_bases
GGGGACGCGACGTACTGTGATCGGGACGTTGAAACTCAGGTTTTAAATGTCCGTATTTACATTCCAGAGAAACTTGCCCG
ACGATAACTGAGACGCTCGG

Downstream 100 bases:

>100_bases
CCAGGATGGCACCCTTCACGCTCCCATCGTGTCATCCATGTTTGCCAATGGCCATGCCGCCTCTCGTAACGCCTCCAGGG
CATCACGCATGGGCACCGGC

Product: lipolytic protein

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MALDPDLAAFLELVEAGISNGVPRLHELPPAKAREQYDISTLALDSPGMEVASVKEIGIPSRDGNEITARLYAPLLDEPL
NGLAAPALLYFHGGGYCVGSLDSHDSLCRTLAALTPCCVLNASYRLAPEHPFPTAVHDAQDAYRWLLSNGLAHGIDPQRI
AVGGDSAGGTLAIGLTIAAREMDWPQPVFQALLYPCTSAWQNTDSHRRFAKGYLLEAATLQWMFSNYLTSERDRTDWRFA
PLEAKDLSSLAPAFIAVAEYDPLVDEGIEYANRLKDAGVPTQLKIYEGMTHDFARLTNILNDASKVREEVAQQLQRAFAA
PRT

Sequences:

>Translated_323_residues
MALDPDLAAFLELVEAGISNGVPRLHELPPAKAREQYDISTLALDSPGMEVASVKEIGIPSRDGNEITARLYAPLLDEPL
NGLAAPALLYFHGGGYCVGSLDSHDSLCRTLAALTPCCVLNASYRLAPEHPFPTAVHDAQDAYRWLLSNGLAHGIDPQRI
AVGGDSAGGTLAIGLTIAAREMDWPQPVFQALLYPCTSAWQNTDSHRRFAKGYLLEAATLQWMFSNYLTSERDRTDWRFA
PLEAKDLSSLAPAFIAVAEYDPLVDEGIEYANRLKDAGVPTQLKIYEGMTHDFARLTNILNDASKVREEVAQQLQRAFAA
PRT
>Mature_322_residues
ALDPDLAAFLELVEAGISNGVPRLHELPPAKAREQYDISTLALDSPGMEVASVKEIGIPSRDGNEITARLYAPLLDEPLN
GLAAPALLYFHGGGYCVGSLDSHDSLCRTLAALTPCCVLNASYRLAPEHPFPTAVHDAQDAYRWLLSNGLAHGIDPQRIA
VGGDSAGGTLAIGLTIAAREMDWPQPVFQALLYPCTSAWQNTDSHRRFAKGYLLEAATLQWMFSNYLTSERDRTDWRFAP
LEAKDLSSLAPAFIAVAEYDPLVDEGIEYANRLKDAGVPTQLKIYEGMTHDFARLTNILNDASKVREEVAQQLQRAFAAP
RT

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=293, Percent_Identity=26.962457337884, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI206597554, Length=278, Percent_Identity=29.4964028776978, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI157041239, Length=274, Percent_Identity=28.1021897810219, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI68051721, Length=295, Percent_Identity=27.7966101694915, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI226423947, Length=303, Percent_Identity=26.7326732673267, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI61966717, Length=263, Percent_Identity=26.9961977186312, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1786682, Length=315, Percent_Identity=28.2539682539683, Blast_Score=112, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71996133, Length=371, Percent_Identity=27.4932614555256, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17540028, Length=336, Percent_Identity=27.3809523809524, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17567059, Length=282, Percent_Identity=27.6595744680851, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI72001146, Length=327, Percent_Identity=25.0764525993884, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI115533410, Length=90, Percent_Identity=37.7777777777778, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI115533412, Length=90, Percent_Identity=37.7777777777778, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24656084, Length=98, Percent_Identity=39.7959183673469, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24656076, Length=98, Percent_Identity=39.7959183673469, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI20130169, Length=98, Percent_Identity=39.7959183673469, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 35265; Mature: 35134

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS01173 LIPASE_GDXG_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALDPDLAAFLELVEAGISNGVPRLHELPPAKAREQYDISTLALDSPGMEVASVKEIGIP
CCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHCCCCCEEEEECCCCCCHHHHHHCCCC
SRDGNEITARLYAPLLDEPLNGLAAPALLYFHGGGYCVGSLDSHDSLCRTLAALTPCCVL
CCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHC
NASYRLAPEHPFPTAVHDAQDAYRWLLSNGLAHGIDPQRIAVGGDSAGGTLAIGLTIAAR
CCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEEEEEEEEC
EMDWPQPVFQALLYPCTSAWQNTDSHRRFAKGYLLEAATLQWMFSNYLTSERDRTDWRFA
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEC
PLEAKDLSSLAPAFIAVAEYDPLVDEGIEYANRLKDAGVPTQLKIYEGMTHDFARLTNIL
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHH
NDASKVREEVAQQLQRAFAAPRT
HHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ALDPDLAAFLELVEAGISNGVPRLHELPPAKAREQYDISTLALDSPGMEVASVKEIGIP
CCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHCCCCCEEEEECCCCCCHHHHHHCCCC
SRDGNEITARLYAPLLDEPLNGLAAPALLYFHGGGYCVGSLDSHDSLCRTLAALTPCCVL
CCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHC
NASYRLAPEHPFPTAVHDAQDAYRWLLSNGLAHGIDPQRIAVGGDSAGGTLAIGLTIAAR
CCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEEEEEEEEC
EMDWPQPVFQALLYPCTSAWQNTDSHRRFAKGYLLEAATLQWMFSNYLTSERDRTDWRFA
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEC
PLEAKDLSSLAPAFIAVAEYDPLVDEGIEYANRLKDAGVPTQLKIYEGMTHDFARLTNIL
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHH
NDASKVREEVAQQLQRAFAAPRT
HHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]