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The map label for this gene is nadB1 [H]

Identifier: 82702866

GI number: 82702866

Start: 1995712

End: 1997316

Strand: Reverse

Name: nadB1 [H]

Synonym: Nmul_A1743

Alternate gene names: 82702866

Gene position: 1997316-1995712 (Counterclockwise)

Preceding gene: 82702867

Following gene: 82702863

Centisome position: 62.72

GC content: 56.82

Gene sequence:

>1605_bases
GTGCCACAGCTTACTTTCGATACTCTGATAATTGGCAGCGGCCTGGCCGGGCTCACTCTTGCGCTCAATCTGGCGCAAAA
CAGGAAAGTGGGCCTCATCACCAAGCGTGCCTTGCTCGATGGGGCAAGCGGGTGGGCGCAGGGAGGAATCGCCGCAACAC
TATCGGAAGAAGACTCACCCGAAGCTCACATCCACGATACGCTTGTCGCAGGTGCGGGTCTTTGCAATGAAGAAGTGACG
CGATATGTGGTGGAAAATGGTCCTCAAGCCGTCCAATGGCTGATTGAGCAGGGAGTGCCTTTCACGCGCGACGGCGGTAA
TGGAACCGGTTATCACCTGACGCGTGAGGGCGGTCATAGCGTTCGCCGCGTCATTCACGCGGCGGATGCGACCGGCAAGG
CAGTGCAATTGACATTGATCGAAAGAGCGCATGCCCACCCCAATATCACCGTGCTCGAGTATCACGTTGCAATCGACCTC
ATAACAAGCGCCAAACTGGCCGGCCGTATCGAACCTCCCTGCAAGAAGGGCAATCGCTGCCTGGGCGCTTATGTGCTGGA
CAGCAGGGAAGGGAGGGTGCGCACCATAGCGGCGAATAACACCGTGCTGGCGACAGGTGGAGCGGGCAAGGTCTATCTTT
ACACTACCAATCCGGATACTGCGACCGGCGATGGCATCGCCATGGGCTGGCGCGCGGGTTGCCGCATAGCGAATATGGAG
TTTATCCAGTTTCATCCAACCTGCCTTTATCATCCTCACGCCAAATCGTTCCTCATCAGCGAAGCGGTGCGAGGCGAGGG
CGGCCTGCTGAAACTGCCGGACGGGACGCGTTTCATGCCCGCGCATGATGAACGTGCGGAACTTGCGCCGCGTGACATCG
TTGCGCGTGCAATTGACTTTGAAATGAAGAAGCGCGGGCTCGACTGTGTGTACCTGGATATCTCGCACAAGCCGGCAAAC
TTCCTGAAGGAGCATTTTCCGACCATTTACCAGCGCTGCCTGGAATTCGGAATTGATATCACCAAAGAACCGATACCTGT
GGTGCCTGCGGCTCACTATACATGTGGCGGTGTCGTCACCGATTGGAACGGCAAGACCGACCTGGACAATCTTTATGCGA
TTGGCGAAACCGCCCACACGGGTCTGCATGGAGCCAACCGCCTGGCAAGCAATTCACTGCTCGAGTGCCTGGTATTCGGG
CAGGCCGCGGCACGGGATATCATGCAGCAGACCCCTGAACCTCTCACCGCATTGCCGCAATGGGATGAAAGCCGCGTGAC
CAATGCCGATGAGGAAATCGTCATTTCCCATAACTGGGATGAACTGCGGCGCTTCATGTGGAGCTACGTTGGCATCGTCC
GCACCACTAAACGTTTACAGCGCGCGCAGCACCGCATCAATCTTTTGCGTGAAGAGATCAACGAATACTACGCGAATTTC
CACGTCACCAACGATCTGCTGGAATTGCGCAACCTGGTGGATACCGCCGGTTTGATCGTACAAAGTGCGATGCTGCGGCA
TGAAAGCCGGGGACTACACTACAGCAAGGATTACCCGGACTTACTGCCGGAGGCAAGGGATACGGTGTTGAAGCGGGGAC
AGTAA

Upstream 100 bases:

>100_bases
GGCGAAAGATCAACCTCTGACCTATCGACTTTGCTGGCGTAAAAGCATCCGCCAGGTCTGTTATCATTCCCTGCTCATAA
GAACAAAATAACGACGACCA

Downstream 100 bases:

>100_bases
AGAGGGCTCTGCTGTAAGAATGATAGTGGGTTTCGTCAATTTCTTGTGCACACTTCTTTCGTATCAATCGAAAACTCGCA
TGGCTGCGGCTTGCCCATCC

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO 1; Quinolinate synthase B 1 [H]

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MPQLTFDTLIIGSGLAGLTLALNLAQNRKVGLITKRALLDGASGWAQGGIAATLSEEDSPEAHIHDTLVAGAGLCNEEVT
RYVVENGPQAVQWLIEQGVPFTRDGGNGTGYHLTREGGHSVRRVIHAADATGKAVQLTLIERAHAHPNITVLEYHVAIDL
ITSAKLAGRIEPPCKKGNRCLGAYVLDSREGRVRTIAANNTVLATGGAGKVYLYTTNPDTATGDGIAMGWRAGCRIANME
FIQFHPTCLYHPHAKSFLISEAVRGEGGLLKLPDGTRFMPAHDERAELAPRDIVARAIDFEMKKRGLDCVYLDISHKPAN
FLKEHFPTIYQRCLEFGIDITKEPIPVVPAAHYTCGGVVTDWNGKTDLDNLYAIGETAHTGLHGANRLASNSLLECLVFG
QAAARDIMQQTPEPLTALPQWDESRVTNADEEIVISHNWDELRRFMWSYVGIVRTTKRLQRAQHRINLLREEINEYYANF
HVTNDLLELRNLVDTAGLIVQSAMLRHESRGLHYSKDYPDLLPEARDTVLKRGQ

Sequences:

>Translated_534_residues
MPQLTFDTLIIGSGLAGLTLALNLAQNRKVGLITKRALLDGASGWAQGGIAATLSEEDSPEAHIHDTLVAGAGLCNEEVT
RYVVENGPQAVQWLIEQGVPFTRDGGNGTGYHLTREGGHSVRRVIHAADATGKAVQLTLIERAHAHPNITVLEYHVAIDL
ITSAKLAGRIEPPCKKGNRCLGAYVLDSREGRVRTIAANNTVLATGGAGKVYLYTTNPDTATGDGIAMGWRAGCRIANME
FIQFHPTCLYHPHAKSFLISEAVRGEGGLLKLPDGTRFMPAHDERAELAPRDIVARAIDFEMKKRGLDCVYLDISHKPAN
FLKEHFPTIYQRCLEFGIDITKEPIPVVPAAHYTCGGVVTDWNGKTDLDNLYAIGETAHTGLHGANRLASNSLLECLVFG
QAAARDIMQQTPEPLTALPQWDESRVTNADEEIVISHNWDELRRFMWSYVGIVRTTKRLQRAQHRINLLREEINEYYANF
HVTNDLLELRNLVDTAGLIVQSAMLRHESRGLHYSKDYPDLLPEARDTVLKRGQ
>Mature_533_residues
PQLTFDTLIIGSGLAGLTLALNLAQNRKVGLITKRALLDGASGWAQGGIAATLSEEDSPEAHIHDTLVAGAGLCNEEVTR
YVVENGPQAVQWLIEQGVPFTRDGGNGTGYHLTREGGHSVRRVIHAADATGKAVQLTLIERAHAHPNITVLEYHVAIDLI
TSAKLAGRIEPPCKKGNRCLGAYVLDSREGRVRTIAANNTVLATGGAGKVYLYTTNPDTATGDGIAMGWRAGCRIANMEF
IQFHPTCLYHPHAKSFLISEAVRGEGGLLKLPDGTRFMPAHDERAELAPRDIVARAIDFEMKKRGLDCVYLDISHKPANF
LKEHFPTIYQRCLEFGIDITKEPIPVVPAAHYTCGGVVTDWNGKTDLDNLYAIGETAHTGLHGANRLASNSLLECLVFGQ
AAARDIMQQTPEPLTALPQWDESRVTNADEEIVISHNWDELRRFMWSYVGIVRTTKRLQRAQHRINLLREEINEYYANFH
VTNDLLELRNLVDTAGLIVQSAMLRHESRGLHYSKDYPDLLPEARDTVLKRGQ

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=513, Percent_Identity=34.8927875243665, Blast_Score=233, Evalue=4e-61,
Organism=Escherichia coli, GI1788928, Length=538, Percent_Identity=59.2936802973978, Blast_Score=654, Evalue=0.0,
Organism=Escherichia coli, GI1790597, Length=531, Percent_Identity=33.3333333333333, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1786942, Length=565, Percent_Identity=32.5663716814159, Blast_Score=209, Evalue=4e-55,
Organism=Caenorhabditis elegans, GI17550100, Length=540, Percent_Identity=33.5185185185185, Blast_Score=230, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI17505833, Length=549, Percent_Identity=32.7868852459016, Blast_Score=224, Evalue=7e-59,
Organism=Caenorhabditis elegans, GI71986328, Length=470, Percent_Identity=28.5106382978723, Blast_Score=114, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6322701, Length=558, Percent_Identity=33.6917562724014, Blast_Score=236, Evalue=5e-63,
Organism=Saccharomyces cerevisiae, GI6322416, Length=519, Percent_Identity=33.3333333333333, Blast_Score=229, Evalue=6e-61,
Organism=Saccharomyces cerevisiae, GI6320788, Length=492, Percent_Identity=28.8617886178862, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17137288, Length=540, Percent_Identity=33.5185185185185, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24655642, Length=540, Percent_Identity=33.5185185185185, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24655647, Length=540, Percent_Identity=33.5185185185185, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24663005, Length=567, Percent_Identity=31.7460317460317, Blast_Score=213, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 58864; Mature: 58732

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQLTFDTLIIGSGLAGLTLALNLAQNRKVGLITKRALLDGASGWAQGGIAATLSEEDSP
CCCCEEEHEEECCCHHHHHHEEEHHCCCCEEEEEHHHHHCCCCCCCCCCEEEEECCCCCC
EAHIHDTLVAGAGLCNEEVTRYVVENGPQAVQWLIEQGVPFTRDGGNGTGYHLTREGGHS
CHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCEECCCCCCCCEEEECCCCHH
VRRVIHAADATGKAVQLTLIERAHAHPNITVLEYHVAIDLITSAKLAGRIEPPCKKGNRC
HHHHHHHCCCCCCEEEEEEEHHHCCCCCEEEEEEEEEEEHHHHHHHCCCCCCCHHCCCEE
LGAYVLDSREGRVRTIAANNTVLATGGAGKVYLYTTNPDTATGDGIAMGWRAGCRIANME
EEEEEEECCCCCEEEEEECCEEEEECCCCEEEEEECCCCCCCCCCEEECHHCCCEEECEE
FIQFHPTCLYHPHAKSFLISEAVRGEGGLLKLPDGTRFMPAHDERAELAPRDIVARAIDF
EEEECCEEEECCCHHHHHHHHHHCCCCCEEECCCCCEECCCCCCHHHCCHHHHHHHHHHH
EMKKRGLDCVYLDISHKPANFLKEHFPTIYQRCLEFGIDITKEPIPVVPAAHYTCGGVVT
HHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCEEE
DWNGKTDLDNLYAIGETAHTGLHGANRLASNSLLECLVFGQAAARDIMQQTPEPLTALPQ
CCCCCCCCCHHEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
WDESRVTNADEEIVISHNWDELRRFMWSYVGIVRTTKRLQRAQHRINLLREEINEYYANF
CCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HVTNDLLELRNLVDTAGLIVQSAMLRHESRGLHYSKDYPDLLPEARDTVLKRGQ
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCHHHHHHHHHCCC
>Mature Secondary Structure 
PQLTFDTLIIGSGLAGLTLALNLAQNRKVGLITKRALLDGASGWAQGGIAATLSEEDSP
CCCEEEHEEECCCHHHHHHEEEHHCCCCEEEEEHHHHHCCCCCCCCCCEEEEECCCCCC
EAHIHDTLVAGAGLCNEEVTRYVVENGPQAVQWLIEQGVPFTRDGGNGTGYHLTREGGHS
CHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCEECCCCCCCCEEEECCCCHH
VRRVIHAADATGKAVQLTLIERAHAHPNITVLEYHVAIDLITSAKLAGRIEPPCKKGNRC
HHHHHHHCCCCCCEEEEEEEHHHCCCCCEEEEEEEEEEEHHHHHHHCCCCCCCHHCCCEE
LGAYVLDSREGRVRTIAANNTVLATGGAGKVYLYTTNPDTATGDGIAMGWRAGCRIANME
EEEEEEECCCCCEEEEEECCEEEEECCCCEEEEEECCCCCCCCCCEEECHHCCCEEECEE
FIQFHPTCLYHPHAKSFLISEAVRGEGGLLKLPDGTRFMPAHDERAELAPRDIVARAIDF
EEEECCEEEECCCHHHHHHHHHHCCCCCEEECCCCCEECCCCCCHHHCCHHHHHHHHHHH
EMKKRGLDCVYLDISHKPANFLKEHFPTIYQRCLEFGIDITKEPIPVVPAAHYTCGGVVT
HHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCEEE
DWNGKTDLDNLYAIGETAHTGLHGANRLASNSLLECLVFGQAAARDIMQQTPEPLTALPQ
CCCCCCCCCHHEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
WDESRVTNADEEIVISHNWDELRRFMWSYVGIVRTTKRLQRAQHRINLLREEINEYYANF
CCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HVTNDLLELRNLVDTAGLIVQSAMLRHESRGLHYSKDYPDLLPEARDTVLKRGQ
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]