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The map label for this gene is ygfZ [C]

Identifier: 82702860

GI number: 82702860

Start: 1989317

End: 1990363

Strand: Reverse

Name: ygfZ [C]

Synonym: Nmul_A1737

Alternate gene names: 82702860

Gene position: 1990363-1989317 (Counterclockwise)

Preceding gene: 82702863

Following gene: 82702859

Centisome position: 62.51

GC content: 54.92

Gene sequence:

>1047_bases
ATGAATCCTGTCTGGCACTCCTACCTGCAACACCGCGGTGCGATTTTCCGGGAAGACTGCATTGTCAGCTACGGCAATGC
TGCTGCCGAACTTGAGAGTACCCGATCTGCCAACGTACTGAGTGATCTTTCTCATTTTGGCCTGATTCACTTTTGGGGAG
AGGATGCGGAAACTTTCCTGCAAGGACAATTGAGTTGCGATATAAGGCGCGCCACTACCTCAACAGCCTCCTACGGGAGC
TATTGCAATCCCAAAGGCAGAATTCTCGCGACTTTTCTGATTTGGCGGACGACTGGTGACGGGTATCTCATGCAGCTGCC
TGCAATCTTGCTCGCGGGGATCCAGAAGCGCCTCGCCATGTATGTGCTGCGGGCCAAGGTCAAATTGGCGGACAGCAGTG
GAGCCTGGGTCCACATAGGGGTCGCGGGCCCTCATGCTGCGGCCCTGTTAAGGAAGATCCTGGGGGAAATTCCTGTCGTG
CCTCTCGGAGTGAGGCATGGCGAAAGAGGAAGCATCATCCGTCTTGCGGAGGATCGCTTTCAACTCCTCATTCTTCCCGA
GCAGGCGCCCACTATATGGGAAGATTTGAGCAGGAATGCGACCCAGGTAGGGAAGCCCTGTTGGGACTGGCTGGAAATCC
GGGCAGGTATTCCCCACATCCTGCCCGCAACCCAGGAACAATTCGTGCCCCAGATGGTGAATCTGGATACTCTCGGGGGG
ATAAGCTTTCAGAAAGGTTGTTATCCCGGCCAGGAAATCGTTGCCCGCACGCAGTATCTCGGCAAAATAAAGCGGCGCAT
GTATCTTGCAAATGTTCGTCCAGCAGCGGGTGATCTGCCAATCGAAGCGGGGGATGAGTTATTCAGCGCCGACTTGGGGG
AACAATCTGCGGGTATGGTAGTCAACCCAGCATCGTCCTCCGACGGAGGAACTGATCTATTGGCTGTCATTCAGACCAGC
AGTGTCGAGGCAGGTGAAATTCACTGGAAATCGCTGGAGGGTCCCGCACTGGGAATTATGCCGCTACCTTATTCCGCGAA
TCAGTAA

Upstream 100 bases:

>100_bases
GATGAGCTGTTATCTCAATGCGGGCAATTTGCCGCCCAGCTCGAGTGTATGCTTTATAATTAACTCTTTTGGGGCTTCTT
TGACCAATACCTGCCTTAAC

Downstream 100 bases:

>100_bases
AGAGTAAAAAAGTATGTGTCCGATTTTGTCATTATCAAATAGCAGCATGTCTCTTGATGAGATCCGAACTCCCCGTCAAA
AAACCACGCAGAAAGAACAT

Product: glycine cleavage T protein (aminomethyl transferase)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MNPVWHSYLQHRGAIFREDCIVSYGNAAAELESTRSANVLSDLSHFGLIHFWGEDAETFLQGQLSCDIRRATTSTASYGS
YCNPKGRILATFLIWRTTGDGYLMQLPAILLAGIQKRLAMYVLRAKVKLADSSGAWVHIGVAGPHAAALLRKILGEIPVV
PLGVRHGERGSIIRLAEDRFQLLILPEQAPTIWEDLSRNATQVGKPCWDWLEIRAGIPHILPATQEQFVPQMVNLDTLGG
ISFQKGCYPGQEIVARTQYLGKIKRRMYLANVRPAAGDLPIEAGDELFSADLGEQSAGMVVNPASSSDGGTDLLAVIQTS
SVEAGEIHWKSLEGPALGIMPLPYSANQ

Sequences:

>Translated_348_residues
MNPVWHSYLQHRGAIFREDCIVSYGNAAAELESTRSANVLSDLSHFGLIHFWGEDAETFLQGQLSCDIRRATTSTASYGS
YCNPKGRILATFLIWRTTGDGYLMQLPAILLAGIQKRLAMYVLRAKVKLADSSGAWVHIGVAGPHAAALLRKILGEIPVV
PLGVRHGERGSIIRLAEDRFQLLILPEQAPTIWEDLSRNATQVGKPCWDWLEIRAGIPHILPATQEQFVPQMVNLDTLGG
ISFQKGCYPGQEIVARTQYLGKIKRRMYLANVRPAAGDLPIEAGDELFSADLGEQSAGMVVNPASSSDGGTDLLAVIQTS
SVEAGEIHWKSLEGPALGIMPLPYSANQ
>Mature_348_residues
MNPVWHSYLQHRGAIFREDCIVSYGNAAAELESTRSANVLSDLSHFGLIHFWGEDAETFLQGQLSCDIRRATTSTASYGS
YCNPKGRILATFLIWRTTGDGYLMQLPAILLAGIQKRLAMYVLRAKVKLADSSGAWVHIGVAGPHAAALLRKILGEIPVV
PLGVRHGERGSIIRLAEDRFQLLILPEQAPTIWEDLSRNATQVGKPCWDWLEIRAGIPHILPATQEQFVPQMVNLDTLGG
ISFQKGCYPGQEIVARTQYLGKIKRRMYLANVRPAAGDLPIEAGDELFSADLGEQSAGMVVNPASSSDGGTDLLAVIQTS
SVEAGEIHWKSLEGPALGIMPLPYSANQ

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

Organism=Escherichia coli, GI1789265, Length=326, Percent_Identity=30.6748466257669, Blast_Score=117, Evalue=9e-28,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: NA

Molecular weight: Translated: 37942; Mature: 37942

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPVWHSYLQHRGAIFREDCIVSYGNAAAELESTRSANVLSDLSHFGLIHFWGEDAETFL
CCCHHHHHHHHCCCEEHHCCEEECCCHHHHHHHHCCHHHHHHHHHCCEEEEECCCHHHHH
QGQLSCDIRRATTSTASYGSYCNPKGRILATFLIWRTTGDGYLMQLPAILLAGIQKRLAM
HCCCCEEEEHHCCCCCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
YVLRAKVKLADSSGAWVHIGVAGPHAAALLRKILGEIPVVPLGVRHGERGSIIRLAEDRF
HHHHHHEEEECCCCCEEEEEECCHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCCE
QLLILPEQAPTIWEDLSRNATQVGKPCWDWLEIRAGIPHILPATQEQFVPQMVNLDTLGG
EEEEECCCCCHHHHHHCCCHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCC
ISFQKGCYPGQEIVARTQYLGKIKRRMYLANVRPAAGDLPIEAGDELFSADLGEQSAGMV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCEE
VNPASSSDGGTDLLAVIQTSSVEAGEIHWKSLEGPALGIMPLPYSANQ
EECCCCCCCCCEEEEEEECCCCCCCCEEEEECCCCEEEEEECCCCCCC
>Mature Secondary Structure
MNPVWHSYLQHRGAIFREDCIVSYGNAAAELESTRSANVLSDLSHFGLIHFWGEDAETFL
CCCHHHHHHHHCCCEEHHCCEEECCCHHHHHHHHCCHHHHHHHHHCCEEEEECCCHHHHH
QGQLSCDIRRATTSTASYGSYCNPKGRILATFLIWRTTGDGYLMQLPAILLAGIQKRLAM
HCCCCEEEEHHCCCCCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
YVLRAKVKLADSSGAWVHIGVAGPHAAALLRKILGEIPVVPLGVRHGERGSIIRLAEDRF
HHHHHHEEEECCCCCEEEEEECCHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCCE
QLLILPEQAPTIWEDLSRNATQVGKPCWDWLEIRAGIPHILPATQEQFVPQMVNLDTLGG
EEEEECCCCCHHHHHHCCCHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCC
ISFQKGCYPGQEIVARTQYLGKIKRRMYLANVRPAAGDLPIEAGDELFSADLGEQSAGMV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCEE
VNPASSSDGGTDLLAVIQTSSVEAGEIHWKSLEGPALGIMPLPYSANQ
EECCCCCCCCCEEEEEEECCCCCCCCEEEEECCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA