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The map label for this gene is glnB [H]

Identifier: 82702764

GI number: 82702764

Start: 1874670

End: 1875008

Strand: Reverse

Name: glnB [H]

Synonym: Nmul_A1638

Alternate gene names: 82702764

Gene position: 1875008-1874670 (Counterclockwise)

Preceding gene: 82702765

Following gene: 82702763

Centisome position: 58.88

GC content: 48.38

Gene sequence:

>339_bases
ATGAAAGAAATCCGCGCATACATTGAGCCCTTCATGTTATCCAAGGTAACGCAGGCGTTACTTGAAATTCCGGATTTCCC
AGGCATGAGTGTCTCGGATTGCCATGGTTTTGGCAGAGAGCGGGTCGCCGATAGCAGTTTCGATCCTTATATGCCGAAAA
AACGCATCGAAATCTTTGCCTCCGATGAGCAGGTGGACAATATTTTCAGCACAGTCATGCAGACCGCCAACACCTATCAG
GACGGCGCAGGCAAAATTTACATCATAGATGTGAGTGGAAGTGGGAATATCAGCAGCGGAGAACGTCATCAGGAAGATTC
CCCTTCCAGACCGGAATAA

Upstream 100 bases:

>100_bases
GGTCATTATCGGCGGGCTCATGAGTTCCACCATTCTTACCCTGACCATGCTTCCTGCGCTCTACTCCAATTTTGAAAAAT
AACCACAACACCCTGCTCTT

Downstream 100 bases:

>100_bases
CAATCATTAAAAGATATAGTTTTCATTTCCAGTATTAATTATTTCGACCTATAAGAACCCTCAAGCACGCCTAAAGCATC
AAATCCTCACCCTTTTCAGT

Product: nitrogen regulatory protein P-II (GlnB, GlnK)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 112; Mature: 112

Protein sequence:

>112_residues
MKEIRAYIEPFMLSKVTQALLEIPDFPGMSVSDCHGFGRERVADSSFDPYMPKKRIEIFASDEQVDNIFSTVMQTANTYQ
DGAGKIYIIDVSGSGNISSGERHQEDSPSRPE

Sequences:

>Translated_112_residues
MKEIRAYIEPFMLSKVTQALLEIPDFPGMSVSDCHGFGRERVADSSFDPYMPKKRIEIFASDEQVDNIFSTVMQTANTYQ
DGAGKIYIIDVSGSGNISSGERHQEDSPSRPE
>Mature_112_residues
MKEIRAYIEPFMLSKVTQALLEIPDFPGMSVSDCHGFGRERVADSSFDPYMPKKRIEIFASDEQVDNIFSTVMQTANTYQ
DGAGKIYIIDVSGSGNISSGERHQEDSPSRPE

Specific function: In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P- II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deur

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1788904, Length=108, Percent_Identity=39.8148148148148, Blast_Score=72, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 12511; Mature: 12511

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEIRAYIEPFMLSKVTQALLEIPDFPGMSVSDCHGFGRERVADSSFDPYMPKKRIEIFA
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHCCCCCCCCCCCCCCEEEEE
SDEQVDNIFSTVMQTANTYQDGAGKIYIIDVSGSGNISSGERHQEDSPSRPE
CCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKEIRAYIEPFMLSKVTQALLEIPDFPGMSVSDCHGFGRERVADSSFDPYMPKKRIEIFA
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHCCCCCCCCCCCCCCEEEEE
SDEQVDNIFSTVMQTANTYQDGAGKIYIIDVSGSGNISSGERHQEDSPSRPE
CCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]