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The map label for this gene is phaD [H]

Identifier: 82702716

GI number: 82702716

Start: 1820841

End: 1822505

Strand: Reverse

Name: phaD [H]

Synonym: Nmul_A1589

Alternate gene names: 82702716

Gene position: 1822505-1820841 (Counterclockwise)

Preceding gene: 82702717

Following gene: 82702715

Centisome position: 57.24

GC content: 57.9

Gene sequence:

>1665_bases
ATGAATGGCTGGGTGAATCATCTTCCGATATTTCCGGTAGTGATCCCATTGGTTGCGGGAGCAGTCATGCTGCTGTTCGC
CGAAGCGCGGCGTGCTCCGCGCACCATTATCGCCCTTGTTGCCACCCTGTTGAATTTACTCGTGGCCCTCGCGCTCGCCT
ATATCGCAAGCGATGCCGTTCCAAGTATCTGGCCGGATGGTATCGCGGTATATCTGCTCGGTGGCTGGCAGGCACCCTTC
GGTATCGTGCTGGTGCTGGATCGATTGTCTGCTCTGATGCTGGTGCTCAACGCGGTGCTGGCATTGACTTCCCTGATCTA
CGCTTTGGCGCGCTGGCGCAAGGCTGGGCCTCATTTCCAATCGCTGTTCCAGTTCCTGATCATGGGGGTAAACGGCGCTT
TTCTGACCGGCGATCTCTTTAACCTGTTTGTATTTGTGGAGGTGCTGCTGGCCGCGTCATACGGTCTCCTGCTGCACGGT
CTGGGGGAACCTCGCGTCAAGGCTGGTCTCCACTATATCGCGATCAATCTTGCAGGTTCGTTTGTCTTTCTGATTGGCGT
TTCGCTGATCTACGGAGTAACCGGAACGCTCAATATGGCGGACGTGGCGGCGCGCGCCTCACTGCTCACCCAGGACGAGC
GTATACTTTTCGAAACCGGTATCGCCATTCTGGGCAGCGCATTTCTGCTCAAGGCGGGGGCATGGCCTCTGAACTTCTGG
CTGCCGTCTGCTTACGGTACCGCTGCGCCTCCAGTGGCAGCGGTTTTTTCGATTATGACCAAGGTGGGGATTTACGCGCT
GCTGCGGCTGGGGTCGCTGCTGTCCGGCGCTTCCGGGGCGAGCGTCCCGGCGCCATTCAACGAGGGCTGGCTGTTTGGCA
TGGGTATCATTACACTCGTGCTGGGAACAGCGGGTATACTCACCGCCAGCCAGCCACAGCGTCTGGTTGCCTATTGTGTC
ATCGCCTCCGCCGGCACGTTGCTTGCAGCGCTCGGGTTTGGCGGTACGGCCATGAAAGGGGCGGCGCTGTATTACTTGAC
AAGCTCAGTGCTGGCCACAGGCGCTTTCTTCATGCTTTGTGAGATGATCGACCGTACACGGCAAACCGGCGAGGACCTGC
CGCTGGAGATGATGGAGCCTGTCGGACAGGAGCCGGTTGATGCAGATTTTACGGATGAAGTGATAGGTATCGTCATTCCC
GCAGCGATGGCATTTCTGGGCATGGGTTTTCTGGCATGCGCGTTGCTCGTGGCAGGCTTGCCGCCACTATCGGGTTTTAT
CGCCAAGTTCCTTATCCTCTCCGCCACACTAACCGATACTGCGCCGGCGCCAGTACCGGTTACGGTATGGGTACTGTGGG
GAGGAATGCTGGGCTCGAGCCTGATTGCAATCATTGCTCTCTGTCGGATGGGAATACATTTGTTCTGGAGCACGGATGAG
ATCATGACGCCAAGACTGCATCTTGGGGAGGCGGTTCCCGTTGCGGTGCTGCTGCTGCTCAGCGTTGCACTTTCGATAGA
GACGGGTCCGGCAATGGAATACTTGAATCTTGCGGGTAATTCACTGTCTTCACCGCAGGTATATATCGATGCGGTGCTGT
CGGGCAGCCGGCCACCCGGGCCCCCGGCAGTACCTTCAATTCCAGTGGAGGGAATCAGCCCATGA

Upstream 100 bases:

>100_bases
CAATCGTCATTGGTTTTGCCATGACTGCACTCTTTCTGGTCGTGTTGCTGGCTTCACGGGGTTTGACGGGCAATGATCAC
GTCGATGGGGATCCGGAAGC

Downstream 100 bases:

>100_bases
AACGCTGGCCATCGCTGACACTATTTACTGTCATTCTGTTTGCCTTGTGGCTTCTGCTGAATGATTCCCTGGCGCCGGGC
CAGCTTTTGCTGGGCCTGAT

Product: putative monovalent cation/H+ antiporter subunit D

Products: NAD+; ubiquinol [C]

Alternate protein names: pH adaptation potassium efflux system protein D; Pha system subunit D [H]

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MNGWVNHLPIFPVVIPLVAGAVMLLFAEARRAPRTIIALVATLLNLLVALALAYIASDAVPSIWPDGIAVYLLGGWQAPF
GIVLVLDRLSALMLVLNAVLALTSLIYALARWRKAGPHFQSLFQFLIMGVNGAFLTGDLFNLFVFVEVLLAASYGLLLHG
LGEPRVKAGLHYIAINLAGSFVFLIGVSLIYGVTGTLNMADVAARASLLTQDERILFETGIAILGSAFLLKAGAWPLNFW
LPSAYGTAAPPVAAVFSIMTKVGIYALLRLGSLLSGASGASVPAPFNEGWLFGMGIITLVLGTAGILTASQPQRLVAYCV
IASAGTLLAALGFGGTAMKGAALYYLTSSVLATGAFFMLCEMIDRTRQTGEDLPLEMMEPVGQEPVDADFTDEVIGIVIP
AAMAFLGMGFLACALLVAGLPPLSGFIAKFLILSATLTDTAPAPVPVTVWVLWGGMLGSSLIAIIALCRMGIHLFWSTDE
IMTPRLHLGEAVPVAVLLLLSVALSIETGPAMEYLNLAGNSLSSPQVYIDAVLSGSRPPGPPAVPSIPVEGISP

Sequences:

>Translated_554_residues
MNGWVNHLPIFPVVIPLVAGAVMLLFAEARRAPRTIIALVATLLNLLVALALAYIASDAVPSIWPDGIAVYLLGGWQAPF
GIVLVLDRLSALMLVLNAVLALTSLIYALARWRKAGPHFQSLFQFLIMGVNGAFLTGDLFNLFVFVEVLLAASYGLLLHG
LGEPRVKAGLHYIAINLAGSFVFLIGVSLIYGVTGTLNMADVAARASLLTQDERILFETGIAILGSAFLLKAGAWPLNFW
LPSAYGTAAPPVAAVFSIMTKVGIYALLRLGSLLSGASGASVPAPFNEGWLFGMGIITLVLGTAGILTASQPQRLVAYCV
IASAGTLLAALGFGGTAMKGAALYYLTSSVLATGAFFMLCEMIDRTRQTGEDLPLEMMEPVGQEPVDADFTDEVIGIVIP
AAMAFLGMGFLACALLVAGLPPLSGFIAKFLILSATLTDTAPAPVPVTVWVLWGGMLGSSLIAIIALCRMGIHLFWSTDE
IMTPRLHLGEAVPVAVLLLLSVALSIETGPAMEYLNLAGNSLSSPQVYIDAVLSGSRPPGPPAVPSIPVEGISP
>Mature_554_residues
MNGWVNHLPIFPVVIPLVAGAVMLLFAEARRAPRTIIALVATLLNLLVALALAYIASDAVPSIWPDGIAVYLLGGWQAPF
GIVLVLDRLSALMLVLNAVLALTSLIYALARWRKAGPHFQSLFQFLIMGVNGAFLTGDLFNLFVFVEVLLAASYGLLLHG
LGEPRVKAGLHYIAINLAGSFVFLIGVSLIYGVTGTLNMADVAARASLLTQDERILFETGIAILGSAFLLKAGAWPLNFW
LPSAYGTAAPPVAAVFSIMTKVGIYALLRLGSLLSGASGASVPAPFNEGWLFGMGIITLVLGTAGILTASQPQRLVAYCV
IASAGTLLAALGFGGTAMKGAALYYLTSSVLATGAFFMLCEMIDRTRQTGEDLPLEMMEPVGQEPVDADFTDEVIGIVIP
AAMAFLGMGFLACALLVAGLPPLSGFIAKFLILSATLTDTAPAPVPVTVWVLWGGMLGSSLIAIIALCRMGIHLFWSTDE
IMTPRLHLGEAVPVAVLLLLSVALSIETGPAMEYLNLAGNSLSSPQVYIDAVLSGSRPPGPPAVPSIPVEGISP

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Escherichia coli, GI1788613, Length=379, Percent_Identity=27.1767810026385, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1788829, Length=454, Percent_Identity=23.568281938326, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 58025; Mature: 58025

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGWVNHLPIFPVVIPLVAGAVMLLFAEARRAPRTIIALVATLLNLLVALALAYIASDAV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PSIWPDGIAVYLLGGWQAPFGIVLVLDRLSALMLVLNAVLALTSLIYALARWRKAGPHFQ
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
SLFQFLIMGVNGAFLTGDLFNLFVFVEVLLAASYGLLLHGLGEPRVKAGLHYIAINLAGS
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCHHEEEEHHHHH
FVFLIGVSLIYGVTGTLNMADVAARASLLTQDERILFETGIAILGSAFLLKAGAWPLNFW
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
LPSAYGTAAPPVAAVFSIMTKVGIYALLRLGSLLSGASGASVPAPFNEGWLFGMGIITLV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
LGTAGILTASQPQRLVAYCVIASAGTLLAALGFGGTAMKGAALYYLTSSVLATGAFFMLC
HHHHHHEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EMIDRTRQTGEDLPLEMMEPVGQEPVDADFTDEVIGIVIPAAMAFLGMGFLACALLVAGL
HHHHHHHHCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PPLSGFIAKFLILSATLTDTAPAPVPVTVWVLWGGMLGSSLIAIIALCRMGIHLFWSTDE
CCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCH
IMTPRLHLGEAVPVAVLLLLSVALSIETGPAMEYLNLAGNSLSSPQVYIDAVLSGSRPPG
HCCCCHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEHHHHCCCCCCC
PPAVPSIPVEGISP
CCCCCCCCCCCCCC
>Mature Secondary Structure
MNGWVNHLPIFPVVIPLVAGAVMLLFAEARRAPRTIIALVATLLNLLVALALAYIASDAV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PSIWPDGIAVYLLGGWQAPFGIVLVLDRLSALMLVLNAVLALTSLIYALARWRKAGPHFQ
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
SLFQFLIMGVNGAFLTGDLFNLFVFVEVLLAASYGLLLHGLGEPRVKAGLHYIAINLAGS
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCHHEEEEHHHHH
FVFLIGVSLIYGVTGTLNMADVAARASLLTQDERILFETGIAILGSAFLLKAGAWPLNFW
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
LPSAYGTAAPPVAAVFSIMTKVGIYALLRLGSLLSGASGASVPAPFNEGWLFGMGIITLV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
LGTAGILTASQPQRLVAYCVIASAGTLLAALGFGGTAMKGAALYYLTSSVLATGAFFMLC
HHHHHHEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EMIDRTRQTGEDLPLEMMEPVGQEPVDADFTDEVIGIVIPAAMAFLGMGFLACALLVAGL
HHHHHHHHCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PPLSGFIAKFLILSATLTDTAPAPVPVTVWVLWGGMLGSSLIAIIALCRMGIHLFWSTDE
CCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCH
IMTPRLHLGEAVPVAVLLLLSVALSIETGPAMEYLNLAGNSLSSPQVYIDAVLSGSRPPG
HCCCCHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEHHHHCCCCCCC
PPAVPSIPVEGISP
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]