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The map label for this gene is phaG [H]

Identifier: 82702713

GI number: 82702713

Start: 1819731

End: 1820081

Strand: Reverse

Name: phaG [H]

Synonym: Nmul_A1586

Alternate gene names: 82702713

Gene position: 1820081-1819731 (Counterclockwise)

Preceding gene: 82702714

Following gene: 82702712

Centisome position: 57.16

GC content: 55.84

Gene sequence:

>351_bases
ATGACGAATGGCGTGATTCCTTTCTGGGCCGACCTGCTCGGCTCGTTGCTGCTGATCTCAGGCAGTCTGTTGACGCTGAT
CGGATCCCTCGGCCTGCTTCGGTTGCCCAACCTGTTTGCGCGAATGCACGGCATAACGTTGGGAAATACACTCGGCCTCG
GGTGTGTGCTGCTGGCATCGATCCTGCTTGCTTCGATACACGCGGAGCGGTTCGTGTTTCAGGAGGTGTTGATCACACTG
TTCATGATATCGACATCGCCTGTCACCACGATCCTGCTCATGCGCTCCGGGATATACCGCAAGCGTGCGGATACCGAACA
AAAACCGCTATCCAGCCAGAATACGAACTGA

Upstream 100 bases:

>100_bases
CCCGATTTTATTTCGACATCGCCCTGCTGATTGCGCTATTCGGGTTTGTCGGGTCAGCCGCAATGGCCAAATTCCTTTTG
CGGGGCGAGGTGATCGAGCC

Downstream 100 bases:

>100_bases
ACCTGATCCTTTCCCCCTTCCGTCCATCTGTTTTACCACTTCCCCTTTTTCCACTGCAACTGAATAGGTGTCCGTCAATC
ATGCTGCCATCCATTGAACA

Product: Na+/H+ antiporter subunit

Products: NA

Alternate protein names: pH adaptation potassium efflux system protein G; Pha system subunit G [H]

Number of amino acids: Translated: 116; Mature: 115

Protein sequence:

>116_residues
MTNGVIPFWADLLGSLLLISGSLLTLIGSLGLLRLPNLFARMHGITLGNTLGLGCVLLASILLASIHAERFVFQEVLITL
FMISTSPVTTILLMRSGIYRKRADTEQKPLSSQNTN

Sequences:

>Translated_116_residues
MTNGVIPFWADLLGSLLLISGSLLTLIGSLGLLRLPNLFARMHGITLGNTLGLGCVLLASILLASIHAERFVFQEVLITL
FMISTSPVTTILLMRSGIYRKRADTEQKPLSSQNTN
>Mature_115_residues
TNGVIPFWADLLGSLLLISGSLLTLIGSLGLLRLPNLFARMHGITLGNTLGLGCVLLASILLASIHAERFVFQEVLITLF
MISTSPVTTILLMRSGIYRKRADTEQKPLSSQNTN

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG1320

COG function: function code P; Multisubunit Na+/H+ antiporter, MnhG subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit G family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005133 [H]

Pfam domain/function: PF03334 PhaG_MnhG_YufB [H]

EC number: NA

Molecular weight: Translated: 12582; Mature: 12451

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNGVIPFWADLLGSLLLISGSLLTLIGSLGLLRLPNLFARMHGITLGNTLGLGCVLLAS
CCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ILLASIHAERFVFQEVLITLFMISTSPVTTILLMRSGIYRKRADTEQKPLSSQNTN
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TNGVIPFWADLLGSLLLISGSLLTLIGSLGLLRLPNLFARMHGITLGNTLGLGCVLLAS
CCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ILLASIHAERFVFQEVLITLFMISTSPVTTILLMRSGIYRKRADTEQKPLSSQNTN
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]