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The map label for this gene is yogA [H]

Identifier: 82702702

GI number: 82702702

Start: 1808631

End: 1809641

Strand: Reverse

Name: yogA [H]

Synonym: Nmul_A1575

Alternate gene names: 82702702

Gene position: 1809641-1808631 (Counterclockwise)

Preceding gene: 82702705

Following gene: 82702700

Centisome position: 56.83

GC content: 54.4

Gene sequence:

>1011_bases
ATGACCAGCAAGGCAATCTATGTGCAACCGGGCGGTGGATTTGATCATGTAACGCTGGGCACATGCGAACCAACGGCGCC
AAAAGCAGGCGAGATTACTGTCAGGATTCGGGCAAGCTCATTGAACTACCATGATTACGCAGTGGTAAGCGGGATGTGGC
CACCCCCTGTGCCACGTATTCCAATGTCCGACGGAGCGGGAGAGGTTGTTGCCGTGGGGGAAGGCGTTAACGAATTTGCA
ATAGGTGACCATGTTGTCAGTACCTTCTTTCCAAGTTGGCAGGAAGGACAACCTGAGATTGATAGCATGGGCACAGTTCC
GGGGGATGGCGTCGATGGTTTTGCGTGCCAGCAAGTGACAATGGCCGCTATCGGTTTCACTCACGCCCCGAAGGGTTATA
GTCATGCGGAGGCAGCTACTCTTACGTGCGCGGGTTTAACAGCGTGGCGGGCACTAATGGTAGACGGTCCATTGAAGCCA
GGCGAAACGGTACTGATTCAGGGAACCGGCGGAGTTTCAGTATTCGCCTTGCAATTCGCGAAGGTAGCAGGAGCAACGGT
AATAGCCACTTCTTCCAGTGACGAGAAACTTGCTCGTCTGGAAACGCTTGGGGCAGATCATCTCATCAATTACCGGAAAC
ATGAAAACTGGGGTGATCTGGTACGTGAATTGACGGCGGGACGCGGGGTCGACCATGTGATTGAAGTGGGAGGGCCTGCC
ACGCTGGCTCAGTCGATGATAGCGACGCGTATTGGCGGACACATCGCCGTAATCGGCATTCTGTCGGGTATGACCGGAAC
GCTGCCGTTGCTCGCTGTGTTGACACGGCAATTGCGCCTTCAGGGGCTGGTCGTTGGCAGCCGCCGTCAGCAAATCGAGA
TGATTCGGGCAATCGAAGCTACCGGGCTGCGGCCGGTCATCGACCGTCATTTTCCACTGGAAGAACTCGTGGAAGCTTTT
CGTTATCAGGAAACCAATCGACACTTTGGCAAGATATGTCTTGATATTTGA

Upstream 100 bases:

>100_bases
TCTGTCTACAACATGGTGTTAATGGTTATTTTGTCCGGGATCAAGTATATATACTAACCATTCTTTTAGACCCAACCAAC
GCAAGAATGAGAGGCAAGCG

Downstream 100 bases:

>100_bases
ATGTAACCATCATTCCGAGTAAGGAATGAAAAAGGAAAATGTCATAATCAGCCGGCATGCCTTTTCACCCAATCGACATA
GCGATTCATCCAGTTCTGCA

Product: zinc-containing alcohol dehydrogenase superfamily protein

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MTSKAIYVQPGGGFDHVTLGTCEPTAPKAGEITVRIRASSLNYHDYAVVSGMWPPPVPRIPMSDGAGEVVAVGEGVNEFA
IGDHVVSTFFPSWQEGQPEIDSMGTVPGDGVDGFACQQVTMAAIGFTHAPKGYSHAEAATLTCAGLTAWRALMVDGPLKP
GETVLIQGTGGVSVFALQFAKVAGATVIATSSSDEKLARLETLGADHLINYRKHENWGDLVRELTAGRGVDHVIEVGGPA
TLAQSMIATRIGGHIAVIGILSGMTGTLPLLAVLTRQLRLQGLVVGSRRQQIEMIRAIEATGLRPVIDRHFPLEELVEAF
RYQETNRHFGKICLDI

Sequences:

>Translated_336_residues
MTSKAIYVQPGGGFDHVTLGTCEPTAPKAGEITVRIRASSLNYHDYAVVSGMWPPPVPRIPMSDGAGEVVAVGEGVNEFA
IGDHVVSTFFPSWQEGQPEIDSMGTVPGDGVDGFACQQVTMAAIGFTHAPKGYSHAEAATLTCAGLTAWRALMVDGPLKP
GETVLIQGTGGVSVFALQFAKVAGATVIATSSSDEKLARLETLGADHLINYRKHENWGDLVRELTAGRGVDHVIEVGGPA
TLAQSMIATRIGGHIAVIGILSGMTGTLPLLAVLTRQLRLQGLVVGSRRQQIEMIRAIEATGLRPVIDRHFPLEELVEAF
RYQETNRHFGKICLDI
>Mature_335_residues
TSKAIYVQPGGGFDHVTLGTCEPTAPKAGEITVRIRASSLNYHDYAVVSGMWPPPVPRIPMSDGAGEVVAVGEGVNEFAI
GDHVVSTFFPSWQEGQPEIDSMGTVPGDGVDGFACQQVTMAAIGFTHAPKGYSHAEAATLTCAGLTAWRALMVDGPLKPG
ETVLIQGTGGVSVFALQFAKVAGATVIATSSSDEKLARLETLGADHLINYRKHENWGDLVRELTAGRGVDHVIEVGGPAT
LAQSMIATRIGGHIAVIGILSGMTGTLPLLAVLTRQLRLQGLVVGSRRQQIEMIRAIEATGLRPVIDRHFPLEELVEAFR
YQETNRHFGKICLDI

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI22538446, Length=350, Percent_Identity=27.1428571428571, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI22538444, Length=350, Percent_Identity=27.1428571428571, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI18379349, Length=354, Percent_Identity=27.9661016949153, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI194239674, Length=305, Percent_Identity=25.5737704918033, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI13236495, Length=305, Percent_Identity=25.5737704918033, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI28557745, Length=344, Percent_Identity=29.0697674418605, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI47519420, Length=348, Percent_Identity=27.0114942528736, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI156627571, Length=307, Percent_Identity=25.7328990228013, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI4501939, Length=358, Percent_Identity=24.3016759776536, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI156523966, Length=358, Percent_Identity=24.3016759776536, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI41872631, Length=339, Percent_Identity=25.9587020648968, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI194239676, Length=302, Percent_Identity=24.5033112582781, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1790485, Length=242, Percent_Identity=30.1652892561983, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788073, Length=349, Percent_Identity=27.2206303724928, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87081918, Length=306, Percent_Identity=27.1241830065359, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790045, Length=321, Percent_Identity=25.5451713395639, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789651, Length=286, Percent_Identity=24.8251748251748, Blast_Score=62, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323729, Length=324, Percent_Identity=27.1604938271605, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324486, Length=324, Percent_Identity=27.1604938271605, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323961, Length=350, Percent_Identity=26, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6319520, Length=348, Percent_Identity=24.7126436781609, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319621, Length=284, Percent_Identity=26.4084507042254, Blast_Score=76, Evalue=9e-15,
Organism=Drosophila melanogaster, GI221330659, Length=222, Percent_Identity=27.9279279279279, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19920632, Length=222, Percent_Identity=27.9279279279279, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24581345, Length=222, Percent_Identity=27.027027027027, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 35715; Mature: 35584

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSKAIYVQPGGGFDHVTLGTCEPTAPKAGEITVRIRASSLNYHDYAVVSGMWPPPVPRI
CCCCEEEEECCCCCCEEEECCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCCCCCC
PMSDGAGEVVAVGEGVNEFAIGDHVVSTFFPSWQEGQPEIDSMGTVPGDGVDGFACQQVT
CCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCCHHHHHHH
MAAIGFTHAPKGYSHAEAATLTCAGLTAWRALMVDGPLKPGETVLIQGTGGVSVFALQFA
HHHHCCCCCCCCCCCCHHHEEEEHHHHHHHHHEECCCCCCCCEEEEECCCCCCHHHHHHH
KVAGATVIATSSSDEKLARLETLGADHLINYRKHENWGDLVRELTAGRGVDHVIEVGGPA
HHCCEEEEEECCCCHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEECCCHH
TLAQSMIATRIGGHIAVIGILSGMTGTLPLLAVLTRQLRLQGLVVGSRRQQIEMIRAIEA
HHHHHHHHHHHCCCEEEEEHHCCCCHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHH
TGLRPVIDRHFPLEELVEAFRYQETNRHFGKICLDI
CCCCHHHHCCCCHHHHHHHHHHHHCCCCCCEEEECC
>Mature Secondary Structure 
TSKAIYVQPGGGFDHVTLGTCEPTAPKAGEITVRIRASSLNYHDYAVVSGMWPPPVPRI
CCCEEEEECCCCCCEEEECCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCCCCCC
PMSDGAGEVVAVGEGVNEFAIGDHVVSTFFPSWQEGQPEIDSMGTVPGDGVDGFACQQVT
CCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCCHHHHHHH
MAAIGFTHAPKGYSHAEAATLTCAGLTAWRALMVDGPLKPGETVLIQGTGGVSVFALQFA
HHHHCCCCCCCCCCCCHHHEEEEHHHHHHHHHEECCCCCCCCEEEEECCCCCCHHHHHHH
KVAGATVIATSSSDEKLARLETLGADHLINYRKHENWGDLVRELTAGRGVDHVIEVGGPA
HHCCEEEEEECCCCHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEECCCHH
TLAQSMIATRIGGHIAVIGILSGMTGTLPLLAVLTRQLRLQGLVVGSRRQQIEMIRAIEA
HHHHHHHHHHHCCCEEEEEHHCCCCHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHH
TGLRPVIDRHFPLEELVEAFRYQETNRHFGKICLDI
CCCCHHHHCCCCHHHHHHHHHHHHCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.26 {1,4-naphthoquinone}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]