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The map label for this gene is oprM [H]

Identifier: 82702631

GI number: 82702631

Start: 1717994

End: 1719505

Strand: Reverse

Name: oprM [H]

Synonym: Nmul_A1502

Alternate gene names: 82702631

Gene position: 1719505-1717994 (Counterclockwise)

Preceding gene: 82702632

Following gene: 82702629

Centisome position: 54.0

GC content: 57.21

Gene sequence:

>1512_bases
ATGATGAATGCGCTGGGCCCTATAAGAGGCGGAATTTATGCCGGCTTGATGCTGGTACTGGGCGGCTGTATGGTGGGGCC
CGATTTTGTACGGCCGAATCCACCCGAGACAGCGCGTTACTTGCCCGGGGAGTCGTTGGAAGGAGCAATTCCTGTAAAGG
ATCAGGCTCAATATTTTGATGCAGGAGCTGACCTCATAGCTGACTGGTGGCGGTTGTTCAAATCGCCCAAGTTGAATACG
CTCGTCACGGAGGGGATCAACAACAACCAGAGTCTCAGGGCAGCGCAGGCGGCATTGCGGCAAAGCCAGCATAATCTTCT
CGCTGGTTATGGCGTATTTTTCCCCCAGGCGGACGCTGCGGGTTCGGCCGTCCGGCAGCGGTTCTCGGCAGCGCGCTTTG
GGGGCAGTACCAGTGCAACATTCAACTTGTTCACATTGTCGACGACGGTTACCTATGCGCTCGATGTATTCGGCGGCCAG
CGGCGCGCCGTGGAGAGTCTCAGGGCACAAAGGGATTTTCAGCGCTATACGGTATTGGCCACTTACCTCACGCTAACCGG
CAATATTGTCAATACTGTGATTGCCAGGGCGGCATACGACGCGCAGATCGAGGCAACAAAAGAGCTTATCGCGCTGCAGC
GGGAGCAAATCGAGATCACCCACGCGCAAATCCGGGCTGGAACAGTTCCATACATCAGTGGAGTCACCCTGCAAACGCAG
CTCGCATCTCTTGAAGCCACACTTCCGGCGCTGGAGCAGGGGCGCGGCCAGGCGGAGCATTTGCTCGACACGCTGATGAA
TTACGCGCCAGGGGAGGGGCTGACGGCCCAGATCAGCCTCGCCGATCTTTCATTGCCCAAAGATCTGCCTGTTACACTGC
CTTCCACGCTCGTGCGCCAGCGCCCTGACATTCTCGCTGCCGAAGCCCAGGTTCATCAATTTACGGCGAACATAGGCGTC
GCGACCGCCAATATGCTTCCAAGCTTCACGTTGAGCGGCACCTATGGACAAAACGCCCGGAACCTCGCGGATGTTTTCAT
GAGCAGTGGAAACTTCTGGAGTGCAGGAGCCGCCTTTGTCGCCCCGATATTCCATGGCGGCACGTTATGGCAGCAGCGAA
AGGCCGCCATTGACGCGTACGACCAGGCGCTTGCCAATTATCGCCAGACCGCTCTGAACGCCCTCGGCCAGATAGCGGAC
CTGCTTCTTGCACTCGAGCACGATGCGGAAACGCTTCAGAGTCAACTGCAGCACCTCGATTCAGCCAGCGAAGCATTGCG
CCTCGTCCGGATCAATCATCAGGCGGGCCTCGTCAATGACCTGCAAGTACTGATCACGAACAGTCAATATCTCCAGGCCA
AGCTCGGCTACATCGGGACTTTGGGCCAGCGCTTCCAGGATACTTCGGGACTATTTGTCGCCTTGGGAGGCGGGTGGTGG
AATGCAGAGGAAGAAGGTGAATTGACGGGGATGGAGGAAATTTTTTTCAATCCGGACTCACCTGTTCCCTAG

Upstream 100 bases:

>100_bases
AGGCGCAGGCAGATGCTGCCATGAAAGAGAAGGAGCAGGAAAGAACAGGAAGCAAGCTATATCAGTCTGAGTAGTCAGCA
GCATCAAACCGAAGGCGGAC

Downstream 100 bases:

>100_bases
CAAAGCCCAAACCAGTCGGTTCCGACCGGAGGTGCCGGAAATCAGCGAAGCGATCTTGTTTTGCCACGCTTGTAGCCCCA
TCGTCCCTCACTTCTCGCTG

Product: RND efflux system outer membrane lipoprotein NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MMNALGPIRGGIYAGLMLVLGGCMVGPDFVRPNPPETARYLPGESLEGAIPVKDQAQYFDAGADLIADWWRLFKSPKLNT
LVTEGINNNQSLRAAQAALRQSQHNLLAGYGVFFPQADAAGSAVRQRFSAARFGGSTSATFNLFTLSTTVTYALDVFGGQ
RRAVESLRAQRDFQRYTVLATYLTLTGNIVNTVIARAAYDAQIEATKELIALQREQIEITHAQIRAGTVPYISGVTLQTQ
LASLEATLPALEQGRGQAEHLLDTLMNYAPGEGLTAQISLADLSLPKDLPVTLPSTLVRQRPDILAAEAQVHQFTANIGV
ATANMLPSFTLSGTYGQNARNLADVFMSSGNFWSAGAAFVAPIFHGGTLWQQRKAAIDAYDQALANYRQTALNALGQIAD
LLLALEHDAETLQSQLQHLDSASEALRLVRINHQAGLVNDLQVLITNSQYLQAKLGYIGTLGQRFQDTSGLFVALGGGWW
NAEEEGELTGMEEIFFNPDSPVP

Sequences:

>Translated_503_residues
MMNALGPIRGGIYAGLMLVLGGCMVGPDFVRPNPPETARYLPGESLEGAIPVKDQAQYFDAGADLIADWWRLFKSPKLNT
LVTEGINNNQSLRAAQAALRQSQHNLLAGYGVFFPQADAAGSAVRQRFSAARFGGSTSATFNLFTLSTTVTYALDVFGGQ
RRAVESLRAQRDFQRYTVLATYLTLTGNIVNTVIARAAYDAQIEATKELIALQREQIEITHAQIRAGTVPYISGVTLQTQ
LASLEATLPALEQGRGQAEHLLDTLMNYAPGEGLTAQISLADLSLPKDLPVTLPSTLVRQRPDILAAEAQVHQFTANIGV
ATANMLPSFTLSGTYGQNARNLADVFMSSGNFWSAGAAFVAPIFHGGTLWQQRKAAIDAYDQALANYRQTALNALGQIAD
LLLALEHDAETLQSQLQHLDSASEALRLVRINHQAGLVNDLQVLITNSQYLQAKLGYIGTLGQRFQDTSGLFVALGGGWW
NAEEEGELTGMEEIFFNPDSPVP
>Mature_503_residues
MMNALGPIRGGIYAGLMLVLGGCMVGPDFVRPNPPETARYLPGESLEGAIPVKDQAQYFDAGADLIADWWRLFKSPKLNT
LVTEGINNNQSLRAAQAALRQSQHNLLAGYGVFFPQADAAGSAVRQRFSAARFGGSTSATFNLFTLSTTVTYALDVFGGQ
RRAVESLRAQRDFQRYTVLATYLTLTGNIVNTVIARAAYDAQIEATKELIALQREQIEITHAQIRAGTVPYISGVTLQTQ
LASLEATLPALEQGRGQAEHLLDTLMNYAPGEGLTAQISLADLSLPKDLPVTLPSTLVRQRPDILAAEAQVHQFTANIGV
ATANMLPSFTLSGTYGQNARNLADVFMSSGNFWSAGAAFVAPIFHGGTLWQQRKAAIDAYDQALANYRQTALNALGQIAD
LLLALEHDAETLQSQLQHLDSASEALRLVRINHQAGLVNDLQVLITNSQYLQAKLGYIGTLGQRFQDTSGLFVALGGGWW
NAEEEGELTGMEEIFFNPDSPVP

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=468, Percent_Identity=25.4273504273504, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI2367348, Length=440, Percent_Identity=23.4090909090909, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 54357; Mature: 54357

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNALGPIRGGIYAGLMLVLGGCMVGPDFVRPNPPETARYLPGESLEGAIPVKDQAQYFD
CCCCCCCHHHHHHHHHHHHHCCHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHH
AGADLIADWWRLFKSPKLNTLVTEGINNNQSLRAAQAALRQSQHNLLAGYGVFFPQADAA
CCHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCEEECCEECCCCCCH
GSAVRQRFSAARFGGSTSATFNLFTLSTTVTYALDVFGGQRRAVESLRAQRDFQRYTVLA
HHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
TYLTLTGNIVNTVIARAAYDAQIEATKELIALQREQIEITHAQIRAGTVPYISGVTLQTQ
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEHHHHCCCCCCCCCCCCHHHH
LASLEATLPALEQGRGQAEHLLDTLMNYAPGEGLTAQISLADLSLPKDLPVTLPSTLVRQ
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHC
RPDILAAEAQVHQFTANIGVATANMLPSFTLSGTYGQNARNLADVFMSSGNFWSAGAAFV
CCCHHHHHHHHHHHHHHCCEEEHHHCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCHHHH
APIFHGGTLWQQRKAAIDAYDQALANYRQTALNALGQIADLLLALEHDAETLQSQLQHLD
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHC
SASEALRLVRINHQAGLVNDLQVLITNSQYLQAKLGYIGTLGQRFQDTSGLFVALGGGWW
CHHHHHHHHEECCCCCCHHCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
NAEEEGELTGMEEIFFNPDSPVP
CCCCCCCCCCCHHHEECCCCCCC
>Mature Secondary Structure
MMNALGPIRGGIYAGLMLVLGGCMVGPDFVRPNPPETARYLPGESLEGAIPVKDQAQYFD
CCCCCCCHHHHHHHHHHHHHCCHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHH
AGADLIADWWRLFKSPKLNTLVTEGINNNQSLRAAQAALRQSQHNLLAGYGVFFPQADAA
CCHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCEEECCEECCCCCCH
GSAVRQRFSAARFGGSTSATFNLFTLSTTVTYALDVFGGQRRAVESLRAQRDFQRYTVLA
HHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
TYLTLTGNIVNTVIARAAYDAQIEATKELIALQREQIEITHAQIRAGTVPYISGVTLQTQ
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEHHHHCCCCCCCCCCCCHHHH
LASLEATLPALEQGRGQAEHLLDTLMNYAPGEGLTAQISLADLSLPKDLPVTLPSTLVRQ
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHC
RPDILAAEAQVHQFTANIGVATANMLPSFTLSGTYGQNARNLADVFMSSGNFWSAGAAFV
CCCHHHHHHHHHHHHHHCCEEEHHHCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCHHHH
APIFHGGTLWQQRKAAIDAYDQALANYRQTALNALGQIADLLLALEHDAETLQSQLQHLD
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHC
SASEALRLVRINHQAGLVNDLQVLITNSQYLQAKLGYIGTLGQRFQDTSGLFVALGGGWW
CHHHHHHHHEECCCCCCHHCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
NAEEEGELTGMEEIFFNPDSPVP
CCCCCCCCCCCHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]