Click here to switch to the map view.

The map label for this gene is glgB

Identifier: 82701850

GI number: 82701850

Start: 829681

End: 831921

Strand: Reverse

Name: glgB

Synonym: Nmul_A0717

Alternate gene names: 82701850

Gene position: 831921-829681 (Counterclockwise)

Preceding gene: 82701853

Following gene: 82701849

Centisome position: 26.13

GC content: 53.9

Gene sequence:

>2241_bases
ATGCGAGCGTATCCTACAATACAGCGCCCCTCCATCAAGCGAGACCCTAGGTTGCATGCCCTGCTGGCTGGACGCCTGCA
CGATCCTTTCAGTTTCCTTGGCCCCCATGTGGAACATGGACATTGGGTGGTGCGCGCCTTCCATCCCCATGCTGCAAGCA
TCTGGATCAACACGGATGACGGTTTCGAAAGCATGACAAGAGCCCATCCTGCCGGAGTTTTTGAATGGTGGGGACTTATA
CCCCTGCCTCTGCCTTATCAGCTACGCATCGAAGAGGCTTCCTTCGAAGCACCCGCACATGTGTATGATATTTATGACGC
CTATGCGTTCCCTCCACAAGTTTCGGAACATGACCTGTATCTGTTCAACGAAGGCCGCTTGCATCAGGCTTACAGGATGC
TCGGTTCTCATTTGGTGGAAATGGAAGGGGTTGCCGGAGTTCGTTTTTCCGTTTGGGCACCGAATGCGGAGCGCGTCAGT
GTGGTCGGAAGCTTTAACCGCTGGGATGGACGTATCCATCCCATGGTCTCCCACGGCGCGAGCGGAGTATGGGAATTGTT
TATCCCCGGCGTGCCGACGGATTCATTTTATAAATATGAAATCCGGAATCGGGATACCGGCGCGATCCTTCTCAAAACGG
ATCCTTATGCGCGCCATTATGAAGTACGTCCCGGCACTGCTGCCCGTGCCCCATCCGCCAGCAGGATGACATGGGGCGAC
GCGCGCTGGATGGCTCAGCGCGCAGTATGGGATTGGCTTGCCACCCCGCTCAATATTTATGAAGTTCATGCCGGGTCATG
GAAACGGCACGCGAACGGAAGTTTTTATTCATACCGGGAACTTGCCGATCACCTGCTCCCGTATGTGCGGGAAATGGGAT
ACACGCATATCGAGTTGCTCCCTATTTCGGAGCATCCCCTCGATGAATCCTGGGGTTATCAGACGACGGGTTATTTTGCC
GCAACCAGTCGCTACGGCACATCGGACGACCTGAAATTTTTCATCAACGCCTGTCATCAGGAGGGAATCGGTGTAATCCT
GGACTGGGTCCCCGCACATTTTCCGCAGGATGCTTTCGCCCTGGCCCGATTCGATGGGAGTGCCCTATATGAACATGAGG
ATCCACGCATGGGACTGCATCAGGACTGGGGCACGCACATTTTCAACTACGGACGCAATGAGGTGAAATCGTTTCTCCTT
TCCAGCGCCCACTACTGGCTATCGGAATTTCATCTTGACGGGCTGCGTGTCGATGCTGTCGCTTCCATGCTCTATCTCGA
CTATTCGCGACTTCCGGGTGAATGGCTGCCCAACAGATATGGAGGCAGGGAGAATCTGGAAGCCATCGATTTTCTGCGTG
AACTGAATATCATGGTGCATCAGGAATTTCCGGGGGCGCTGACACTTGCCGAGGAATCCACTGCATGGCCGGGTGTATCC
CGTCCGACTTATGTCGGCGGGCTGGGTTTTTCCATGAAGTGGAACATGGGCTGGATGAACGACACTCTGGTGTATATGCG
CAATGATCCCGTCTACCGGCGTTTCCATCATGAGCAGTTGACGTTCGGGCAGTTATACGCATACAGCGAAAACTTTGTGC
TGCCGTTCTCCCATGATGAAGTGGTTCACGGCAAAGGTTCGCTTCTGGGCAAGATGCCTGGAGATGCATGGCAACAATTC
GCCAACCTGCGCCTGCTGTTTACCTATCAGATGACATATCCCGGGAAAAAACTCAACTTCATGGGGAATGAATTTGCCCA
GGGGCGTGAGTGGAATTCATCGCGCGAACTCGACTGGTATCAGTTAGGCGATGGCTGGCATGAGGGAGTAAAAATGGCAG
CGCGAGACCTCAACCAACTGTACAGTGCCGTCGCCGCACTGCATCAACGTGATTTCGAGCCGGAAGGCTTTTCGTGGGTG
GATTGTCACGACGCGGATAACTCTGTGATCAGTTATCTGCGCTTTGCACGAAACGGTTCGTTTGCTCTGGTGGTACTCAA
TTTTACGCCGGTGCCACGCTACCGTTACCGCATCGGGGTGCCCACATCGGGAAGCTATCAGGAAATCTTCAATAGCGATT
CTCGTTACTATGGGGGCAGCGATCTCGGTAACATGGGAAGTATCCGCACCACCGGCCAGCCTTCCATGGAACGGATGGAT
TCAATTGTTATCACCCTGCCGCCGCTTGCCGGGCTCATTTTCGTTCATACTGATCTGCCTGCAATCAACACCGACGGATA
A

Upstream 100 bases:

>100_bases
CAGTTTCTTGGCAAAAATCTCGCGCCCATTGTGTAAATCGGTCCTGCTCGTATGCAGTTGCCGCCCTCGCTACCCTGACT
ATCCACGTTATAATCGATAC

Downstream 100 bases:

>100_bases
GGGAGCAGGTTGATCCTCGAGACAAGTCCATAGCCCTGGGAACCCCTGAACAAATCCCTCACAGGCGCGACGGAGGCTTT
GTTTAGAGTTTCCCTGGTAA

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 746; Mature: 746

Protein sequence:

>746_residues
MRAYPTIQRPSIKRDPRLHALLAGRLHDPFSFLGPHVEHGHWVVRAFHPHAASIWINTDDGFESMTRAHPAGVFEWWGLI
PLPLPYQLRIEEASFEAPAHVYDIYDAYAFPPQVSEHDLYLFNEGRLHQAYRMLGSHLVEMEGVAGVRFSVWAPNAERVS
VVGSFNRWDGRIHPMVSHGASGVWELFIPGVPTDSFYKYEIRNRDTGAILLKTDPYARHYEVRPGTAARAPSASRMTWGD
ARWMAQRAVWDWLATPLNIYEVHAGSWKRHANGSFYSYRELADHLLPYVREMGYTHIELLPISEHPLDESWGYQTTGYFA
ATSRYGTSDDLKFFINACHQEGIGVILDWVPAHFPQDAFALARFDGSALYEHEDPRMGLHQDWGTHIFNYGRNEVKSFLL
SSAHYWLSEFHLDGLRVDAVASMLYLDYSRLPGEWLPNRYGGRENLEAIDFLRELNIMVHQEFPGALTLAEESTAWPGVS
RPTYVGGLGFSMKWNMGWMNDTLVYMRNDPVYRRFHHEQLTFGQLYAYSENFVLPFSHDEVVHGKGSLLGKMPGDAWQQF
ANLRLLFTYQMTYPGKKLNFMGNEFAQGREWNSSRELDWYQLGDGWHEGVKMAARDLNQLYSAVAALHQRDFEPEGFSWV
DCHDADNSVISYLRFARNGSFALVVLNFTPVPRYRYRIGVPTSGSYQEIFNSDSRYYGGSDLGNMGSIRTTGQPSMERMD
SIVITLPPLAGLIFVHTDLPAINTDG

Sequences:

>Translated_746_residues
MRAYPTIQRPSIKRDPRLHALLAGRLHDPFSFLGPHVEHGHWVVRAFHPHAASIWINTDDGFESMTRAHPAGVFEWWGLI
PLPLPYQLRIEEASFEAPAHVYDIYDAYAFPPQVSEHDLYLFNEGRLHQAYRMLGSHLVEMEGVAGVRFSVWAPNAERVS
VVGSFNRWDGRIHPMVSHGASGVWELFIPGVPTDSFYKYEIRNRDTGAILLKTDPYARHYEVRPGTAARAPSASRMTWGD
ARWMAQRAVWDWLATPLNIYEVHAGSWKRHANGSFYSYRELADHLLPYVREMGYTHIELLPISEHPLDESWGYQTTGYFA
ATSRYGTSDDLKFFINACHQEGIGVILDWVPAHFPQDAFALARFDGSALYEHEDPRMGLHQDWGTHIFNYGRNEVKSFLL
SSAHYWLSEFHLDGLRVDAVASMLYLDYSRLPGEWLPNRYGGRENLEAIDFLRELNIMVHQEFPGALTLAEESTAWPGVS
RPTYVGGLGFSMKWNMGWMNDTLVYMRNDPVYRRFHHEQLTFGQLYAYSENFVLPFSHDEVVHGKGSLLGKMPGDAWQQF
ANLRLLFTYQMTYPGKKLNFMGNEFAQGREWNSSRELDWYQLGDGWHEGVKMAARDLNQLYSAVAALHQRDFEPEGFSWV
DCHDADNSVISYLRFARNGSFALVVLNFTPVPRYRYRIGVPTSGSYQEIFNSDSRYYGGSDLGNMGSIRTTGQPSMERMD
SIVITLPPLAGLIFVHTDLPAINTDG
>Mature_746_residues
MRAYPTIQRPSIKRDPRLHALLAGRLHDPFSFLGPHVEHGHWVVRAFHPHAASIWINTDDGFESMTRAHPAGVFEWWGLI
PLPLPYQLRIEEASFEAPAHVYDIYDAYAFPPQVSEHDLYLFNEGRLHQAYRMLGSHLVEMEGVAGVRFSVWAPNAERVS
VVGSFNRWDGRIHPMVSHGASGVWELFIPGVPTDSFYKYEIRNRDTGAILLKTDPYARHYEVRPGTAARAPSASRMTWGD
ARWMAQRAVWDWLATPLNIYEVHAGSWKRHANGSFYSYRELADHLLPYVREMGYTHIELLPISEHPLDESWGYQTTGYFA
ATSRYGTSDDLKFFINACHQEGIGVILDWVPAHFPQDAFALARFDGSALYEHEDPRMGLHQDWGTHIFNYGRNEVKSFLL
SSAHYWLSEFHLDGLRVDAVASMLYLDYSRLPGEWLPNRYGGRENLEAIDFLRELNIMVHQEFPGALTLAEESTAWPGVS
RPTYVGGLGFSMKWNMGWMNDTLVYMRNDPVYRRFHHEQLTFGQLYAYSENFVLPFSHDEVVHGKGSLLGKMPGDAWQQF
ANLRLLFTYQMTYPGKKLNFMGNEFAQGREWNSSRELDWYQLGDGWHEGVKMAARDLNQLYSAVAALHQRDFEPEGFSWV
DCHDADNSVISYLRFARNGSFALVVLNFTPVPRYRYRIGVPTSGSYQEIFNSDSRYYGGSDLGNMGSIRTTGQPSMERMD
SIVITLPPLAGLIFVHTDLPAINTDG

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=678, Percent_Identity=29.646017699115, Blast_Score=194, Evalue=2e-49,
Organism=Escherichia coli, GI1789839, Length=734, Percent_Identity=51.7711171662125, Blast_Score=729, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554896, Length=678, Percent_Identity=26.8436578171091, Blast_Score=194, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI32564391, Length=536, Percent_Identity=28.544776119403, Blast_Score=174, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6320826, Length=669, Percent_Identity=26.7563527653214, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI28573410, Length=626, Percent_Identity=28.7539936102236, Blast_Score=189, Evalue=5e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_NITMU (Q2YB47)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411416.1
- ProteinModelPortal:   Q2YB47
- SMR:   Q2YB47
- STRING:   Q2YB47
- GeneID:   3786063
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0717
- eggNOG:   COG0296
- HOGENOM:   HBG287139
- OMA:   WMATRAA
- PhylomeDB:   Q2YB47
- ProtClustDB:   PRK05402
- BioCyc:   NMUL323848:NMUL_A0717-MONOMER
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: =2.4.1.18

Molecular weight: Translated: 85380; Mature: 85380

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: ACT_SITE 313-313 ACT_SITE 348-348 ACT_SITE 353-353 ACT_SITE 416-416 ACT_SITE 418-418 ACT_SITE 471-471 ACT_SITE 538-538 ACT_SITE 539-539

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAYPTIQRPSIKRDPRLHALLAGRLHDPFSFLGPHVEHGHWVVRAFHPHAASIWINTDD
CCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHCCCCCCCCEEEEEECCCEEEEEEECCC
GFESMTRAHPAGVFEWWGLIPLPLPYQLRIEEASFEAPAHVYDIYDAYAFPPQVSEHDLY
CHHHHHHCCCCCHHHHCCCCCCCCCCEEEEECCCCCCCCCEEEHHHHHCCCCCCCCCCEE
LFNEGRLHQAYRMLGSHLVEMEGVAGVRFSVWAPNAERVSVVGSFNRWDGRIHPMVSHGA
EECCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCCCEECHHHHCCC
SGVWELFIPGVPTDSFYKYEIRNRDTGAILLKTDPYARHYEVRPGTAARAPSASRMTWGD
CCEEEEEECCCCCCCEEEEEECCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCH
ARWMAQRAVWDWLATPLNIYEVHAGSWKRHANGSFYSYRELADHLLPYVREMGYTHIELL
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEE
PISEHPLDESWGYQTTGYFAATSRYGTSDDLKFFINACHQEGIGVILDWVPAHFPQDAFA
ECCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEE
LARFDGSALYEHEDPRMGLHQDWGTHIFNYGRNEVKSFLLSSAHYWLSEFHLDGLRVDAV
EEECCCCEEECCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEHHHH
ASMLYLDYSRLPGEWLPNRYGGRENLEAIDFLRELNIMVHQEFPGALTLAEESTAWPGVS
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCEEEEECCCCCCCCCC
RPTYVGGLGFSMKWNMGWMNDTLVYMRNDPVYRRFHHEQLTFGQLYAYSENFVLPFSHDE
CCCEEECCCEEEEECCCCCCCEEEEEECCCHHHHHHHCCCCEEEEEEECCCEEEECCCCC
VVHGKGSLLGKMPGDAWQQFANLRLLFTYQMTYPGKKLNFMGNEFAQGREWNSSRELDWY
EECCCCCEEECCCCHHHHHHCCEEEEEEEEEECCCCEEECCCCHHHCCCCCCCCCCCCEE
QLGDGWHEGVKMAARDLNQLYSAVAALHQRDFEPEGFSWVDCHDADNSVISYLRFARNGS
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHCCCC
FALVVLNFTPVPRYRYRIGVPTSGSYQEIFNSDSRYYGGSDLGNMGSIRTTGQPSMERMD
EEEEEEECCCCCCEEEEECCCCCCCHHHHHCCCCCEECCCCCCCCCCCCCCCCHHHHHHC
SIVITLPPLAGLIFVHTDLPAINTDG
EEEEEECCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure
MRAYPTIQRPSIKRDPRLHALLAGRLHDPFSFLGPHVEHGHWVVRAFHPHAASIWINTDD
CCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHCCCCCCCCEEEEEECCCEEEEEEECCC
GFESMTRAHPAGVFEWWGLIPLPLPYQLRIEEASFEAPAHVYDIYDAYAFPPQVSEHDLY
CHHHHHHCCCCCHHHHCCCCCCCCCCEEEEECCCCCCCCCEEEHHHHHCCCCCCCCCCEE
LFNEGRLHQAYRMLGSHLVEMEGVAGVRFSVWAPNAERVSVVGSFNRWDGRIHPMVSHGA
EECCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCCCEECHHHHCCC
SGVWELFIPGVPTDSFYKYEIRNRDTGAILLKTDPYARHYEVRPGTAARAPSASRMTWGD
CCEEEEEECCCCCCCEEEEEECCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCH
ARWMAQRAVWDWLATPLNIYEVHAGSWKRHANGSFYSYRELADHLLPYVREMGYTHIELL
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEE
PISEHPLDESWGYQTTGYFAATSRYGTSDDLKFFINACHQEGIGVILDWVPAHFPQDAFA
ECCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEE
LARFDGSALYEHEDPRMGLHQDWGTHIFNYGRNEVKSFLLSSAHYWLSEFHLDGLRVDAV
EEECCCCEEECCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEHHHH
ASMLYLDYSRLPGEWLPNRYGGRENLEAIDFLRELNIMVHQEFPGALTLAEESTAWPGVS
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCEEEEECCCCCCCCCC
RPTYVGGLGFSMKWNMGWMNDTLVYMRNDPVYRRFHHEQLTFGQLYAYSENFVLPFSHDE
CCCEEECCCEEEEECCCCCCCEEEEEECCCHHHHHHHCCCCEEEEEEECCCEEEECCCCC
VVHGKGSLLGKMPGDAWQQFANLRLLFTYQMTYPGKKLNFMGNEFAQGREWNSSRELDWY
EECCCCCEEECCCCHHHHHHCCEEEEEEEEEECCCCEEECCCCHHHCCCCCCCCCCCCEE
QLGDGWHEGVKMAARDLNQLYSAVAALHQRDFEPEGFSWVDCHDADNSVISYLRFARNGS
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHCCCC
FALVVLNFTPVPRYRYRIGVPTSGSYQEIFNSDSRYYGGSDLGNMGSIRTTGQPSMERMD
EEEEEEECCCCCCEEEEECCCCCCCHHHHHCCCCCEECCCCCCCCCCCCCCCCHHHHHHC
SIVITLPPLAGLIFVHTDLPAINTDG
EEEEEECCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA