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The map label for this gene is fdoH

Identifier: 82546239

GI number: 82546239

Start: 3934135

End: 3935037

Strand: Reverse

Name: fdoH

Synonym: SBO_3907

Alternate gene names: 82546239

Gene position: 3935037-3934135 (Counterclockwise)

Preceding gene: 82546240

Following gene: 82546238

Centisome position: 87.06

GC content: 55.59

Gene sequence:

>903_bases
ATGGCTTATCAATCTCAAGATATCATTCGTCGTTCCGCGACTAACGGTCTGACCCCCGCGCCTCAGGCGCGGGACTTCCA
GGAAGAAGTGGCGAAACTCATCGACGTCACCACCTGTATCGGCTGTAAAGCCTGTCAGGTGGCGTGTTCAGAGTGGAACG
ACATTCGCGATACCGTCGGCAATAACATTGGGGTGTACGACAACCCCAATGATTTAAGTGCCAAATCGTGGACGGTGATG
CGCTTCTCGGAAGTGGAGCAGAACGACAAACTGGAATGGCTGATCCGCAAAGACGGCTGTATGCACTGTTCCGATCCAGG
CTGTCTGAAAGCGTGCCCGGCGGAAGGTGCAATTATTCAGTATGCCAACGGTATCGTCGACTTCCAGTCCGAGCAGTGCA
TTGGCTGCGGTTATTGCATTGCGGGCTGTCCGTTCGACATTCCGCGCCTCAACCCGGAAGACAACCGCGTCTACAAATGT
ACGCTGTGCGTTGACCGCGTGGTGGTTGGGCAAGAACCAGCCTGCGTGAAGACCTGCCCAACGGGCGCGATTCACTTCGG
TACGAAAGAGTCGATGAAAACGCTGGCGAGCGAGCGCGTGGCGGAGCTGAAAACCCGCGGTTACGACAATGCGGGCCTGT
ACGATCCGGCAGGCGTCGGTGGTACACACGTTATGTACGTGCTGCACCATGCTGACAAGCCAAATCTGTATCATGGCTTG
CCGGAGAACCCGGAAATCAGCGAAACCGTGAAATTCTGGAAAGGCATCTGGAAACCGCTCGCAGCTGTTGGCTTTGCGGC
TACCTTCGCAGCCAGTATCTTCCACTACGTGGGTGTCGGTCCGAACCGTGCGGATGAGGAAGAGAATAATCTGCACGAAG
AGAAAGACGAGGAGCGCAAATGA

Upstream 100 bases:

>100_bases
TGCCAATACTTTAACGCCATTCGTTGGTGATGCGAACACGCAGACGCCGGAGTTTAAGTCCTTCCTTGTGAATGTGGAAA
AGGTGTAACGGAGACGACAT

Downstream 100 bases:

>100_bases
AACGACGTGACACCATCGTGCGCTACACCGCGCCGGAACGTATCAACCACTGGATCACCGCCTTCTGCTTCATCCTAGCG
GCGGTGAGCGGGCTGGGCTT

Product: formate dehydrogenase-O, iron-sulfur subunit

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase iron-sulfur subunit; FDH-Z subunit beta; Formate dehydrogenase-O subunit beta

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKACQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVM
RFSEVEQNDKLEWLIRKDGCMHCSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAGVGGTHVMYVLHHADKPNLYHGL
PENPEISETVKFWKGIWKPLAAVGFAATFAASIFHYVGVGPNRADEEENNLHEEKDEERK

Sequences:

>Translated_300_residues
MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKACQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVM
RFSEVEQNDKLEWLIRKDGCMHCSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAGVGGTHVMYVLHHADKPNLYHGL
PENPEISETVKFWKGIWKPLAAVGFAATFAASIFHYVGVGPNRADEEENNLHEEKDEERK
>Mature_299_residues
AYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKACQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMR
FSEVEQNDKLEWLIRKDGCMHCSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKCT
LCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAGVGGTHVMYVLHHADKPNLYHGLP
ENPEISETVKFWKGIWKPLAAVGFAATFAASIFHYVGVGPNRADEEENNLHEEKDEERK

Specific function: Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron- sulfur centers. Electrons are transferred from the gamma chain to the m

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Escherichia coli, GI1790326, Length=300, Percent_Identity=100, Blast_Score=629, Evalue=0.0,
Organism=Escherichia coli, GI1787749, Length=294, Percent_Identity=77.5510204081633, Blast_Score=493, Evalue=1e-141,
Organism=Escherichia coli, GI1789370, Length=184, Percent_Identity=33.1521739130435, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1787872, Length=167, Percent_Identity=32.3353293413174, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787122, Length=161, Percent_Identity=31.6770186335404, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI2367345, Length=178, Percent_Identity=29.7752808988764, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI226510944, Length=181, Percent_Identity=29.8342541436464, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87082179, Length=172, Percent_Identity=27.906976744186, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787963, Length=148, Percent_Identity=24.3243243243243, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788811, Length=168, Percent_Identity=24.4047619047619, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787740, Length=112, Percent_Identity=28.5714285714286, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787478, Length=87, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FDOH_ECOL6 (P0AAJ6)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_756698.1
- ProteinModelPortal:   P0AAJ6
- SMR:   P0AAJ6
- PRIDE:   P0AAJ6
- EnsemblBacteria:   EBESCT00000044899
- GeneID:   1040442
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c4843
- GeneTree:   EBGT00070000031697
- HOGENOM:   HBG726839
- OMA:   PSPGAII
- ProtClustDB:   CLSK880047
- GO:   GO:0006810
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246
- PIRSF:   PIRSF036298
- TIGRFAMs:   TIGR01582

Pfam domain/function: PF09163 Form-deh_trans

EC number: 1.2.1.2

Molecular weight: Translated: 33101; Mature: 32969

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1ac4a410)-;

Cys/Met content:

5.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
5.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKACQVACSEWNDIRDTVG
CCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHC
NNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMHCSDPGCLKACPAEGAIIQ
CCCCCCCCCCCCCCCCEEEEEHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEE
YANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKCTLCVDRVVVGQEPACVKTCP
ECCCEEECCCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEEEEHHHHCCCCCCCEEECC
TGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAGVGGTHVMYVLHHADKPNLYHGL
CCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCC
PENPEISETVKFWKGIWKPLAAVGFAATFAASIFHYVGVGPNRADEEENNLHEEKDEERK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCH
>Mature Secondary Structure 
AYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKACQVACSEWNDIRDTVG
CCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHC
NNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMHCSDPGCLKACPAEGAIIQ
CCCCCCCCCCCCCCCCEEEEEHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEE
YANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKCTLCVDRVVVGQEPACVKTCP
ECCCEEECCCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEEEEHHHHCCCCCCCEEECC
TGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAGVGGTHVMYVLHHADKPNLYHGL
CCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCC
PENPEISETVKFWKGIWKPLAAVGFAATFAASIFHYVGVGPNRADEEENNLHEEKDEERK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157