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The map label for this gene is rfaH

Identifier: 82546193

GI number: 82546193

Start: 3877769

End: 3878257

Strand: Reverse

Name: rfaH

Synonym: SBO_3854

Alternate gene names: 82546193

Gene position: 3878257-3877769 (Counterclockwise)

Preceding gene: 82546196

Following gene: 82546183

Centisome position: 85.81

GC content: 49.28

Gene sequence:

>489_bases
ATGCAATCCTGGTATTTACTGTACTGCAAGCGCGGGCAACTTCAACGTGCCCAGGAACACCTCGAAAGACAGGCTGTGAA
TTGCCTGGCACCGATGATCACCCTGGAAAAAATCGTGCGTGGAAAACGTACTGCAGTCAGTGAGCCATTGTTTCCCAACT
ACCTGTTTGTCGAATTTGATCCAGAAGTGATTCATACCACGACTATCAACGCGACCCGCGGTGTCAGCCACTTCGTGCGC
TTTGGCGCGTCGCCAGCGATAGTCCCATCGGCGGTGATTCATCAGCTATCGGTATATAAACCGAAAGACATTGTCGATCC
GGCAACACCTTATCCGGGTGATAAGGTGATTATTACCGAAGGCGCGTTCGAAGGCTTTCAGGCCATTTTCACCGAACCCG
ATGGTGAGGCTCGTTCCATGCTATTGCTTAATCTTATTAATAAAGAGATTAAGCACAGTGTGAAGAACACCGAGTTCCGC
AAACTCTAA

Upstream 100 bases:

>100_bases
AGCGTGAACTCTGACGGTATAACGCAAACCGGGGAATATAATTAACTTAGCGTAAAGCTTTTGCTATCCTTGCGCCCCGA
TTAAACGGATAAGAGTCATT

Downstream 100 bases:

>100_bases
AACGCAATCCCAAACAGTGTTTTGACATTGGCATCCGTGGTGGCAGCCAGCCATGCGGCATCTTCTCCACGCCAGTGCGC
AATACGTTGCAAAATATGGT

Product: transcriptional activator RfaH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRTAVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVR
FGASPAIVPSAVIHQLSVYKPKDIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
KL

Sequences:

>Translated_162_residues
MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRTAVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVR
FGASPAIVPSAVIHQLSVYKPKDIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
KL
>Mature_162_residues
MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRTAVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVR
FGASPAIVPSAVIHQLSVYKPKDIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
KL

Specific function: Transcriptional activator of hemolysin synthesis and secretion, also required for expression of sex factor and lipopolysaccharide biosynthesis genes. Transcriptional activator of rfaD/htrM and htrL

COG id: COG0250

COG function: function code K; Transcription antiterminator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790276, Length=162, Percent_Identity=100, Blast_Score=338, Evalue=1e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFAH_ECO57 (P0AFW1)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B91225
- PIR:   H86071
- RefSeq:   NP_290472.1
- RefSeq:   NP_312797.1
- ProteinModelPortal:   P0AFW1
- SMR:   P0AFW1
- EnsemblBacteria:   EBESCT00000023913
- EnsemblBacteria:   EBESCT00000057766
- GeneID:   915134
- GeneID:   960318
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5362
- KEGG:   ecs:ECs4770
- GeneTree:   EBGT00050000011661
- HOGENOM:   HBG751162
- OMA:   CKRGQLL
- ProtClustDB:   PRK09014
- BioCyc:   ECOL83334:ECS4770-MONOMER
- GO:   GO:0006350
- InterPro:   IPR005824
- InterPro:   IPR010215
- InterPro:   IPR006645
- InterPro:   IPR014722
- Gene3D:   G3DSA:2.30.30.30
- Gene3D:   G3DSA:3.30.70.940
- SMART:   SM00739
- SMART:   SM00738
- TIGRFAMs:   TIGR01955

Pfam domain/function: PF02357 NusG; SSF82679 Transcrpt_antiterm_NusG_N

EC number: NA

Molecular weight: Translated: 18340; Mature: 18340

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRTAVSEPLFPNYLFVEFD
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEEEEC
PEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPKDIVDPATPYPGDKVIITE
CCEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEC
GAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFRKL
CCCCCHHHEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRTAVSEPLFPNYLFVEFD
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEEEEC
PEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPKDIVDPATPYPGDKVIITE
CCEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEC
GAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFRKL
CCCCCHHHEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796