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The map label for this gene is metR

Identifier: 82546179

GI number: 82546179

Start: 3861991

End: 3862944

Strand: Reverse

Name: metR

Synonym: SBO_3840

Alternate gene names: 82546179

Gene position: 3862944-3861991 (Counterclockwise)

Preceding gene: 82546183

Following gene: 82546175

Centisome position: 85.47

GC content: 55.77

Gene sequence:

>954_bases
ATGATCGAAGTAAAACATCTGAAAACGCTACAAGCGTTGCGGAACTGCGGCTCACTCGCAGCCGCTGCGGCGACGTTGCA
TCAAACGCAATCCGCCCTGTCTCACCAGTTTAGCGATCTGGAACAACGCCTTGGCTTCCGGCTATTTGTGCGTAAAAGCC
AGCCGCTTCGCTTTACACCGCAGGGAGAAATCCTGTTGCAACTGGCAAACCAGGTGCTGCCACAAATTAGCCAGGCACTG
CAAGCCTGCAATGAACCGCAGCAGACGCGTCTGCGCATTGCCATTGAGTGCCATAGCTGTATACAGTGGCTGACGCCCGC
GTTAGAAAATTTCCATAAGAACTGGCCGCAGGTCGAGATGGACTTTAAATCGGGCGTGACATTTGACCCGCAGCCCGCCT
TGCAACAGGGAGAGCTGGATCTGGTAATGACGTCCGATATTCTGCCGCGCAGTGGTCTGCATTATTCGCCGATGTTCGAC
TATGAAGTGCGTCTGGTGTTAGCACCTGACCATCCACTGGCGGCAAAAACGCGTATTACACCGGAAGATCTCGCCAGCGA
GACGCTATTAATTTATCCGGTGCAGCGTAGTCGACTGGATGTCTGGCGGCATTTTCTTCAGCCGGCAGGCGTCAGTCCGT
CACTGAAAAGCGTCGATAACACCTTATTGTTGATTCAGATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGG
GTAGTAGAGAGTTTTGAGCGCCAGGGTCTGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGC
CGTGCGCGATGGCGAGCAGCGTCAGCCAGTTACGGAAGCGTTTATTCGCTCAGCGCGCAATCACGCCTGCGATCATCTGC
CGTTTGTGAAGAGCGCGGAGCGACCCACTTACGATGCACCCACAGTGAGGCCAGGATCACCAGCGCGCCTGTAA

Upstream 100 bases:

>100_bases
GACGAAGGATTTTAGCCGTCCAGATGTTTACACATCCATAATTGGCGGTTACTGTATATTCCTCAAGCGCAAATTATTCA
TGCCGAAGTGAAGGACTTTC

Downstream 100 bases:

>100_bases
TCAACGTTGGCCAGTGCGGCTGTTGGTGCCAGATAGCCAGGTTTACCAGCAGCCCTGCCGGAACGTGCATATTATTCATA
ATTCCCAGCGTTCCGGCGTC

Product: MetR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRLGFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQAL
QACNEPQQTRLRIAIECHSCIQWLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSVDNTLLLIQMVAARMGIAALPHW
VVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQPVTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL

Sequences:

>Translated_317_residues
MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRLGFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQAL
QACNEPQQTRLRIAIECHSCIQWLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSVDNTLLLIQMVAARMGIAALPHW
VVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQPVTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL
>Mature_317_residues
MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRLGFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQAL
QACNEPQQTRLRIAIECHSCIQWLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSVDNTLLLIQMVAARMGIAALPHW
VVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQPVTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes. It is also a negative regulator of its own expression

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1790262, Length=317, Percent_Identity=100, Blast_Score=652, Evalue=0.0,
Organism=Escherichia coli, GI157672245, Length=236, Percent_Identity=24.5762711864407, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1787806, Length=290, Percent_Identity=23.448275862069, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788887, Length=286, Percent_Identity=26.9230769230769, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87082024, Length=274, Percent_Identity=28.4671532846715, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787530, Length=207, Percent_Identity=27.536231884058, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1787879, Length=254, Percent_Identity=25.5905511811024, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87081904, Length=219, Percent_Identity=28.7671232876712, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1790399, Length=255, Percent_Identity=27.843137254902, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1789204, Length=264, Percent_Identity=25.7575757575758, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790208, Length=299, Percent_Identity=25.0836120401338, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1786401, Length=245, Percent_Identity=24.4897959183673, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786448, Length=207, Percent_Identity=26.5700483091787, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789173, Length=176, Percent_Identity=28.4090909090909, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1789506, Length=190, Percent_Identity=24.2105263157895, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): METR_ECO57 (P0A9G0)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D86070
- PIR:   F91223
- RefSeq:   NP_290460.1
- RefSeq:   NP_312785.1
- ProteinModelPortal:   P0A9G0
- SMR:   P0A9G0
- EnsemblBacteria:   EBESCT00000029106
- EnsemblBacteria:   EBESCT00000057070
- GeneID:   915146
- GeneID:   960330
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5349
- KEGG:   ecs:ECs4758
- GeneTree:   EBGT00050000008328
- HOGENOM:   HBG685425
- OMA:   EQRQPVI
- ProtClustDB:   PRK15421
- BioCyc:   ECOL83334:ECS4758-MONOMER
- GO:   GO:0005737
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00039

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 35629; Mature: 35629

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRLGFRLFVRKSQPLRFTP
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEECC
QGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQWLTPALENFHKNWPQVEM
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEHHHHHHHHHHHHHHHHCCCCCEEE
DFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFDYEVRLVLAPDHPLAAKTRIT
HHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCC
PEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSVDNTLLLIQMVAARMGIAALPHW
HHHHCCCEEEEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQPVTEAFIRSARNHACDHLPFVKSAE
HHHHHHHCCEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCC
RPTYDAPTVRPGSPARL
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRLGFRLFVRKSQPLRFTP
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEECC
QGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQWLTPALENFHKNWPQVEM
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEHHHHHHHHHHHHHHHHCCCCCEEE
DFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFDYEVRLVLAPDHPLAAKTRIT
HHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCC
PEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSVDNTLLLIQMVAARMGIAALPHW
HHHHCCCEEEEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQPVTEAFIRSARNHACDHLPFVKSAE
HHHHHHHCCEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCC
RPTYDAPTVRPGSPARL
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796