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The map label for this gene is recG

Identifier: 82546079

GI number: 82546079

Start: 3741021

End: 3743102

Strand: Reverse

Name: recG

Synonym: SBO_3725

Alternate gene names: 82546079

Gene position: 3743102-3741021 (Counterclockwise)

Preceding gene: 82546080

Following gene: 82546077

Centisome position: 82.82

GC content: 56.82

Gene sequence:

>2082_bases
ATGACAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAGCACTTAGTAACAAACTGGCGAA
AATCAACCTGCATACCGTGCAGGATCTACTCTTACACCTTCCCCTGCGCTACGAAGATCGCACCCATCTCTACCCCATCG
GAGAACTACTGCCGGGCGTTTATGCCACGGTGGAAGGCGAAGTGCTGAACTGCAATATCTCCTTCGGCGGTCGGCGGATG
ATGACCTGCCAGATCAGCGACGGTTCCGGCATCCTCACCATGCGCTTTTTCAATTTCAGCGCGGCAATGAAAAATAGCCT
GGCGACGGGCCGCCGTGTACTGGCTTATGGCGAAGCAAAGCGCGGTAAATATGGTGCGGAGATGATCCACCCGGAATACC
GCGTGCAGGGCGATCTCAGCACGCCAGAATTACAGGAAACGCTCACGCCGGTTTATCCAACAACGGAAGGCGTAAAGCAG
GCCACGCTGCGTAAATTAACCGACCAGGCGCTGGATCTGCTCGACACCTGCGCCATTGAAGAACTCCTGCCGCCGGAACT
GTCACAAGGAATGATGACGCTACCGGAAGCGTTGCGCACTTTGCACCGCCCGCCACCGACGCTACAGCTTAGCGATCTGG
AAACCGGGCAGCATCCGGCGCAACGTCGTCTGATTCTGGAAGAACTGCTGGCGCACAACCTCAGCATGTTAGCCTTACGT
GCCGGAGCACAGCGTTTTCATGCCCAGCCGCTGAGCGCCAATGATGCGCTGAAAAATAAACTCCTCGCAGCCTTACCGTT
CAAGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGCCTGG
TACAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGCTGCGTGCGATTGCCCACGGCAAACAGGTG
GCATTGATGGCACCAACCGAATTACTCGCCGAACAGCACGCCAATAACTTCCGCAACTGGTTTGAACCGCTCGGTATCGA
AGTGGGCTGGCTCGCCGGTAAGCAGAAAGGCAAAGCGCGACTGTCTCAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGA
TGATTGTTGGTACGCACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGTCTGGCGCTGGTGATTATCGACGAACAACAT
CGCTTTGGCGTGCATCAGCGTCTGGCGTTATGGGAGAAAGGCCAGCAGCAAGGCTTCCATCCGCATCAGTTGATCATGAC
CGCCACGCCGATCCCCCGCACGCTGGCAATGACGGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAG
GCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCGCCATGCCTGCATA
ACTGAAGGTCGTCAGGCGTACTGGGTTTGTACGTTGATTGAAGAGTCAGAGTTACTGGAAGCGCAGGCGGCGGAAGCTAC
CTGGGAAGAGTTGAAACTGGCGCTACCAGAATTGAATGTTGGCCTGGTACACGGGCGGATGAAACCAGCCGAGAAACAGG
CGGTGATGGCGTCGTTTAAACAAGGTGAGCTACATCTGCTGGTTGCCACTACCGTTATTGAAGTCGGTGTTGATGTGCCT
AACGCCAGCCTGATGATTATCGAAAACCCTGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCG
TGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGCGCAAATTCGCCTGCAAGTGCTGC
GCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCACGCGTCAG
ACGGGTAATGCTGAATTTAAAGTGGCGGATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCGCGACA
TATTCACGAACGTTACCCACAACAGGCAAAAGCCTTGATAGAACGCTGGATGCCGGAGACAGAACGTTACTCGAATGCGT
AA

Upstream 100 bases:

>100_bases
CAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGTGGGCTACTATG
CAGGCTGCAGGGTAAGTGCC

Downstream 100 bases:

>100_bases
AAGGCGGCGGTGAGAAGACCGCCGCTTCATCGGTTAACCGGCAAAAATCGGCAACATCAAATACAACTTAATTACCAGCG
CATTGACGATATCGATAAAG

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 693; Mature: 692

Protein sequence:

>693_residues
MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRYEDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRM
MTCQISDGSGILTMRFFNFSAAMKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQHPAQRRLILEELLAHNLSMLALR
AGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARVVAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQV
ALMAPTELLAEQHANNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLALVIIDEQH
RFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDELPPGRTPVTTVAIPDTRRTDIIDRVRHACI
TEGRQAYWVCTLIEESELLEAQAAEATWEELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVP
NASLMIIENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQKDLEIRGPGELLGTRQ
TGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIERWMPETERYSNA

Sequences:

>Translated_693_residues
MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRYEDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRM
MTCQISDGSGILTMRFFNFSAAMKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQHPAQRRLILEELLAHNLSMLALR
AGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARVVAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQV
ALMAPTELLAEQHANNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLALVIIDEQH
RFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDELPPGRTPVTTVAIPDTRRTDIIDRVRHACI
TEGRQAYWVCTLIEESELLEAQAAEATWEELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVP
NASLMIIENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQKDLEIRGPGELLGTRQ
TGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIERWMPETERYSNA
>Mature_692_residues
TGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRYEDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMM
TCQISDGSGILTMRFFNFSAAMKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQA
TLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQHPAQRRLILEELLAHNLSMLALRA
GAQRFHAQPLSANDALKNKLLAALPFKPTGAQARVVAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVA
LMAPTELLAEQHANNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLALVIIDEQHR
FGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDELPPGRTPVTTVAIPDTRRTDIIDRVRHACIT
EGRQAYWVCTLIEESELLEAQAAEATWEELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPN
ASLMIIENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQKDLEIRGPGELLGTRQT
GNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIERWMPETERYSNA

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain

Homologues:

Organism=Escherichia coli, GI2367254, Length=693, Percent_Identity=99.1341991341991, Blast_Score=1402, Evalue=0.0,
Organism=Escherichia coli, GI1787357, Length=429, Percent_Identity=37.2960372960373, Blast_Score=240, Evalue=2e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECG_ECO57 (Q8XD86)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   G91194
- PIR:   H86041
- RefSeq:   NP_290232.2
- RefSeq:   NP_312554.1
- ProteinModelPortal:   Q8XD86
- SMR:   Q8XD86
- EnsemblBacteria:   EBESCT00000028460
- EnsemblBacteria:   EBESCT00000056623
- GeneID:   915516
- GeneID:   960893
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5078
- KEGG:   ecs:ECs4527
- GeneTree:   EBGT00050000009356
- HOGENOM:   HBG747311
- OMA:   VLGTKQT
- ProtClustDB:   PRK10917
- BioCyc:   ECOL83334:ECS4527-MONOMER
- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027
- InterPro:   IPR004365
- SMART:   SM00487
- SMART:   SM00490
- TIGRFAMs:   TIGR00643

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =3.6.4.12

Molecular weight: Translated: 76498; Mature: 76366

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS51192 HELICASE_ATP_BIND_1; PS51194 HELICASE_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRYEDRTHLYPIGELLPGV
CCCCCCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCCCCCEEEHHHHCCCC
YATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAAMKNSLATGRRVLAYGEAK
EEEECCEEEEEEEECCCCEEEEEEECCCCCEEEEEEECHHHHHHHHHHCCCEEEEECCCC
RGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQATLRKLTDQALDLLDTCAIE
CCCCCHHHCCCCCEEECCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQHPAQRRLILEELLAHNLSMLALR
HHCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHH
AGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARVVAEIERDMALDVPMMRLVQGDVGS
HHHHHHCCCCCCCCHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCC
GKTLVAALAALRAIAHGKQVALMAPTELLAEQHANNFRNWFEPLGIEVGWLAGKQKGKAR
CHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHH
LSQQEAIASGQVQMIVGTHAIFQEQVQFNGLALVIIDEQHRFGVHQRLALWEKGQQQGFH
HHHHHHHHCCCEEEEECHHHHHHHHHHHCCEEEEEECCCHHCCHHHHHHHHHHHHHCCCC
PHQLIMTATPIPRTLAMTAYADLDTSVIDELPPGRTPVTTVAIPDTRRTDIIDRVRHACI
CCEEEEECCCCCHHHHHHHHHCCCHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
TEGRQAYWVCTLIEESELLEAQAAEATWEELKLALPELNVGLVHGRMKPAEKQAVMASFK
HCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCC
QGELHLLVATTVIEVGVDVPNASLMIIENPERLGLAQLHQLRGRVGRGAVASHCVLLYKT
CCCEEEEEEEEHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCHHHCEEEEEEC
PLSKTAQIRLQVLRDSNDGFVIAQKDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPE
CCCCHHHEEEEEEECCCCCEEEEECCCEECCCHHHCCCCCCCCCCEEHHHHHHHHHCCHH
VQRLARHIHERYPQQAKALIERWMPETERYSNA
HHHHHHHHHHHCCHHHHHHHHHCCCCHHHCCCC
>Mature Secondary Structure 
TGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRYEDRTHLYPIGELLPGV
CCCCCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCCCCCEEEHHHHCCCC
YATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAAMKNSLATGRRVLAYGEAK
EEEECCEEEEEEEECCCCEEEEEEECCCCCEEEEEEECHHHHHHHHHHCCCEEEEECCCC
RGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQATLRKLTDQALDLLDTCAIE
CCCCCHHHCCCCCEEECCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQHPAQRRLILEELLAHNLSMLALR
HHCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHH
AGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARVVAEIERDMALDVPMMRLVQGDVGS
HHHHHHCCCCCCCCHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCC
GKTLVAALAALRAIAHGKQVALMAPTELLAEQHANNFRNWFEPLGIEVGWLAGKQKGKAR
CHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHH
LSQQEAIASGQVQMIVGTHAIFQEQVQFNGLALVIIDEQHRFGVHQRLALWEKGQQQGFH
HHHHHHHHCCCEEEEECHHHHHHHHHHHCCEEEEEECCCHHCCHHHHHHHHHHHHHCCCC
PHQLIMTATPIPRTLAMTAYADLDTSVIDELPPGRTPVTTVAIPDTRRTDIIDRVRHACI
CCEEEEECCCCCHHHHHHHHHCCCHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
TEGRQAYWVCTLIEESELLEAQAAEATWEELKLALPELNVGLVHGRMKPAEKQAVMASFK
HCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCC
QGELHLLVATTVIEVGVDVPNASLMIIENPERLGLAQLHQLRGRVGRGAVASHCVLLYKT
CCCEEEEEEEEHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCHHHCEEEEEEC
PLSKTAQIRLQVLRDSNDGFVIAQKDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPE
CCCCHHHEEEEEEECCCCCEEEEECCCEECCCHHHCCCCCCCCCCEEHHHHHHHHHCCHH
VQRLARHIHERYPQQAKALIERWMPETERYSNA
HHHHHHHHHHHCCHHHHHHHHHCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796