Definition Shigella boydii Sb227, complete genome.
Accession NC_007613
Length 4,519,823

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The map label for this gene is gpsA [H]

Identifier: 82545976

GI number: 82545976

Start: 3631427

End: 3632446

Strand: Reverse

Name: gpsA [H]

Synonym: SBO_3614

Alternate gene names: 82545976

Gene position: 3632446-3631427 (Counterclockwise)

Preceding gene: 82545977

Following gene: 82545975

Centisome position: 80.37

GC content: 56.57

Gene sequence:

>1020_bases
ATGAACCAACGTAATGCTTCAATGACTGTGATCGGTGCCGGCTCGTACGGCACCGCTCTTGCCATTACCCTGGCAAGAAA
TGGCCACGAGGTTGTCCTCTGGGGCCATGACCCTGAACATATCGCAACGCTTGAACGCGACCGCTGTAACGCCGCGTTTC
TCCCCGATGTGCCTTTTCCCGATACGCTCCATCTTGAAAGCGATCTCGCCACTGCGCTGGCAGCCAGCCGTAATATTCTC
GTCGTCGTACCCAGCCATGTCTTTGGTGAAGTGCTGCGCCAGATTAAACCGCTGATGCGTCCTGATGCGCGTCTGGTGTG
GGCGACCAAAGGGCTGGAAGCGGAAACCGGACGTCTGTTACAGGACGTGGCGCGTGAGGCCTTAGGCGATCAAATTCCGC
TGGCGGTTATCTCTGGCCCAACGTTTGCGAAAGAACTGGCGGCAGGTTTACCGACGGCTATTTCGTTGGCCTCTACCGAC
CAGACCTTTGCCGATGATCTCCAACAATTGCTGCACTGTGGCAAAAGTTTCCGCGTTTACAGCAACCCGGATTTCATCGG
TGTGCAGCTTGGCGGTGCGGTGAAAAACGTCATTGCCATTGGCGCGGGGATGTCCGACGGTATCGGTTTTGGTGCGAATG
CGCGTACGGCACTGATCACCCGTGGGCTGGCTGAAATGTCGCGTCTTGGCGCGGCGCTGGGTGCCGAGCCTGCCACCTTT
ATGGGCATGGCGGGGCTGGGCGATCTTGTGCTTACCTGTACCGACAACCAGTCGCGTAACCGCCGTTTTGGCATGATGCT
CGGTCAGGGCATGGATGTACAAAGCGCGCAGGAGAAGATTGGTCAGGTGGTGGAAGGCTACCGCAATACGAAAGAAGTCC
GCGAACTGGCGCATCGCTTCGGCGTAGAAATGCCAATAACCGAGGAAATTTATCAAGTATTATATTGCGGAAAAAACGCG
CGCGAGGCAGCATTGACGTTATTAGGTCGTGCACGCAAGGACGAGCGCAGCAGCCACTAA

Upstream 100 bases:

>100_bases
TCCCGCAACTGAACCTTGCGCCGGTTAACTTCGATGCGCTGTTCATGAACTATTTGCAGCAGCAGGCTGGCGAAGGTACT
GAAGAACATCAGGATGCCTG

Downstream 100 bases:

>100_bases
CCCCAGGGAACCTTTGTTACCGCTATGACCCGGCCCGCGCAGAACGGGCCGGTCATTATCTCATCGTGTGGAGTAAGTAA
TGTCGTGTGAAGAACTGGAA

Product: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase

Products: NA

Alternate protein names: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATLERDRCNAAFLPDVPFPDTLHLESDLATALAASRNIL
VVVPSHVFGEVLRQIKPLMRPDARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTALITRGLAEMSRLGAALGAEPATF
MGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSAQEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNA
REAALTLLGRARKDERSSH

Sequences:

>Translated_339_residues
MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATLERDRCNAAFLPDVPFPDTLHLESDLATALAASRNIL
VVVPSHVFGEVLRQIKPLMRPDARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTALITRGLAEMSRLGAALGAEPATF
MGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSAQEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNA
REAALTLLGRARKDERSSH
>Mature_339_residues
MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATLERDRCNAAFLPDVPFPDTLHLESDLATALAASRNIL
VVVPSHVFGEVLRQIKPLMRPDARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTALITRGLAEMSRLGAALGAEPATF
MGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSAQEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNA
REAALTLLGRARKDERSSH

Specific function: De novo phospholipid biosynthesis; glycerol-3 phosphate formation. [C]

COG id: COG0240

COG function: function code C; Glycerol-3-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI33695088, Length=342, Percent_Identity=26.3157894736842, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI24307999, Length=338, Percent_Identity=25.4437869822485, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1790037, Length=339, Percent_Identity=99.7050147492625, Blast_Score=692, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17507425, Length=336, Percent_Identity=23.2142857142857, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI32564399, Length=344, Percent_Identity=25, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI32564403, Length=353, Percent_Identity=24.0793201133144, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI193210136, Length=354, Percent_Identity=24.0112994350282, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI193210134, Length=218, Percent_Identity=27.9816513761468, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324513, Length=340, Percent_Identity=26.7647058823529, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320181, Length=345, Percent_Identity=27.536231884058, Blast_Score=106, Evalue=6e-24,
Organism=Drosophila melanogaster, GI17136204, Length=331, Percent_Identity=25.6797583081571, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI17136202, Length=331, Percent_Identity=25.6797583081571, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI17136200, Length=331, Percent_Identity=25.6797583081571, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI22026922, Length=352, Percent_Identity=23.5795454545455, Blast_Score=85, Evalue=7e-17,
Organism=Drosophila melanogaster, GI281362270, Length=239, Percent_Identity=28.0334728033473, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI45551945, Length=239, Percent_Identity=28.0334728033473, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24648969, Length=183, Percent_Identity=28.9617486338798, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR006168
- InterPro:   IPR006109
- InterPro:   IPR011128
- InterPro:   IPR016040 [H]

Pfam domain/function: PF07479 NAD_Gly3P_dh_C; PF01210 NAD_Gly3P_dh_N [H]

EC number: =1.1.1.94 [H]

Molecular weight: Translated: 36376; Mature: 36376

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00957 NAD_G3PDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATLERDRCNAAFLPDVPFP
CCCCCCEEEEEECCCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHCCCCEEECCCCCCC
DTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPDARLVWATKGLEAETGRLL
CCEECHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
QDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTDQTFADDLQQLLHCGKSFRVY
HHHHHHHCCCCCCEEEECCCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEE
SNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTALITRGLAEMSRLGAALGAEPATF
CCCCEEEEEECHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
MGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSAQEKIGQVVEGYRNTKEVRELAHRF
HHHCCCCCEEEEECCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHC
GVEMPITEEIYQVLYCGKNAREAALTLLGRARKDERSSH
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATLERDRCNAAFLPDVPFP
CCCCCCEEEEEECCCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHCCCCEEECCCCCCC
DTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPDARLVWATKGLEAETGRLL
CCEECHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
QDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTDQTFADDLQQLLHCGKSFRVY
HHHHHHHCCCCCCEEEECCCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEE
SNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTALITRGLAEMSRLGAALGAEPATF
CCCCEEEEEECHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
MGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSAQEKIGQVVEGYRNTKEVRELAHRF
HHHCCCCCEEEEECCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHC
GVEMPITEEIYQVLYCGKNAREAALTLLGRARKDERSSH
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA