BacMap

Definition	Shigella boydii Sb227, complete genome.
Accession	NC_007613
Length	4,519,823

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The map label for this gene is selB [H]

Identifier: 82545952

GI number: 82545952

Start: 3598919

End: 3600763

Strand: Reverse

Name: selB [H]

Synonym: SBO_3590

Alternate gene names: 82545952

Gene position: 3600763-3598919 (Counterclockwise)

Preceding gene: 82545953

Following gene: 82545951

Centisome position: 79.67

GC content: 56.04

Gene sequence:

>1845_bases
ATGATTATTGCGACTGCCGGACACGTTGACCACGGCAAAACAACCTTATTGCAGGCGATTACTGGCGTAAATGCTGACCG
TCTGCCGGAAGAAAAAAAGCGCGGCATGACCATCGATCTCGGCTATGCCTACTGGCCGCAGCCGGATGGTCGCGTGCCTA
GTTTTATCGACGTACCCGGTCATGAAAAGTTTCTTTCCAACATGCTGGCGGGCGTTGGTGGTATCGATCACGCGCTGTTG
GTGGTGGCGTGCGATGACGGCGTGATGGCGCAGACCCGTGAGCATCTGGCGATTTTGCAGCTGACCGGTAACCCGATGCT
GACAGTGGCGCTGACCAAGGCCGATCGCGTGGACGAAGCGCGTGTTGATGAGGTTGAACGCCAGGTAAAGGAGGTGCTGC
GGGAATACGGTTTTGCTGAGGCAAAACTGTTTATCACCGCAGCAACCGAAGGTCGGGGAATTGATGCCCTGCGCGAGCAT
CTGCTTCAGTTACCAGGACGTGAACACGCCAGCCAACATAGTTTCCGCCTCGCGATCGACCGCGCATTTACCGTAAAAGG
TGCCGGGCTGGTCGTCACCGGTACGGCGTTAAGCGGGGAAGTGAAGGTAGGCGATTCACTCTGGCTGACTGGTGTAAATA
AACCGATGCGTGTACGTGCGCTGCATGCACAAAACCAGCCAACAGAAACCGCCCATGCCGGGCAGCGTATCGCGCTTAAC
ATCGCGGGTGATGCGGAAAAAGAGCAGATTAACCGTGGCGACTGGCTGCTTGCCGATGCGCCGCCAGAGCCGTTCACACG
GGTGATTGTCGAGCTTCAAACCCATACACCGCTGACCCAGTGGCAGCCGCTGCATATTCACCACGCCGCCAGCCACGTCA
CGGGACGCGTTTCACTGCTGGAAGATAACCTTGCTGAACTGGTCTTCGACACCCCGTTATGGCTGGCAGATAACGACCGC
CTGGTATTGCGCGATATCTCTGCCCGCAACACGCTGGCCGGAGCGCGCGTCGTGATGCTTAACCCGCCGCGTCGCGGTAA
ACGTAAACCAGAGTATCTGCAATGGCTGGCTTCCCTCGCCAGGGCACAGAATGATAGCGAAGCGTTAGCCGTTCACCTGC
AACGCGGCGCGGTTAATCTTCCTGATTTCGCCTGGGCGCGCCAGCTCAACGGCGAAGGGATGCGCGAACTGCTGCAACAG
CCTGGCTATATTCAGGCTGGTTATAGCTTGTTGAATGCGCCGGTTGCCGCTCGCTGGCAACGGAAAATTCTCGACACATT
AGCGACTTATCATGAGCAACATCGCGATGAACCTGGGCCTGGGCGCGAACGTCTGCGACGTATGGCGTTGCCAATGGAAG
ATGAAGCGCTAGTACTGTTGCTGATTGAAAAGATGCGCGAAAGCGGCGACATCCTCAGTCATCACGGTTGGCTGCATCTG
CCGGATCACAAAGCGGGCTTCAGCGAAGAGCAGCAGGCCATCTGGCAAAAAGCAGAGCCGCTGTTTAGTGACGAGCCGTG
GTGGGTGCGTGATCTGGCAAAAGAGACGGGTACCGACGAGCAGGCAATGCGCTTGACGCTAAGCCAGGCGGCGCAGCAAG
GAATAATTACCGCGATCGTCAAAGATCGTTATTACCGTAACGATCGGATTGTCGAATTTGCCAATATGATCCGCGATCTC
GATCAGGAGTGTGGTTCAACCTGCGCGGCGGATTTCCGCGATCGCTTAGGCGTAGGCCGAAAGCTGGCAATTCAGATTCT
GGAATATTTTGACCGCATTGGCTTTACGCGTCGTCGTGGAAATGATCATTTATTGCGCGACGCATTATTATTTCCGGAAA
AATAA

Upstream 100 bases:

>100_bases
AATTGCCAGTGCCGGTGATTGGTCGTATTTATGACGGACGATTGTGGCTGGATTTACGCTGCCTTGAAGATGAGCAACGG
TTTTTGGAGATGTTGTTGAA

Downstream 100 bases:

>100_bases
GGAAATGATTAATTAATTTTTAAAATAAATTAATACAAATTTCTTATGATTTTAAAAAAAGCACATTGTTTAATGAATAC
AATGTGCTTTTTATTAGATT

Product: selenocysteinyl-tRNA-specific translation factor

Products: NA

Alternate protein names: SelB translation factor [H]

Number of amino acids: Translated: 614; Mature: 614

Protein sequence:

>614_residues
MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAYWPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALL
VVACDDGVMAQTREHLAILQLTGNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
LLQLPGREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRVRALHAQNQPTETAHAGQRIALN
IAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHTPLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDR
LVLRDISARNTLAGARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGEGMRELLQQ
PGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMALPMEDEALVLLLIEKMRESGDILSHHGWLHL
PDHKAGFSEEQQAIWQKAEPLFSDEPWWVRDLAKETGTDEQAMRLTLSQAAQQGIITAIVKDRYYRNDRIVEFANMIRDL
DQECGSTCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK

Sequences:

>Translated_614_residues
MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAYWPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALL
VVACDDGVMAQTREHLAILQLTGNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
LLQLPGREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRVRALHAQNQPTETAHAGQRIALN
IAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHTPLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDR
LVLRDISARNTLAGARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGEGMRELLQQ
PGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMALPMEDEALVLLLIEKMRESGDILSHHGWLHL
PDHKAGFSEEQQAIWQKAEPLFSDEPWWVRDLAKETGTDEQAMRLTLSQAAQQGIITAIVKDRYYRNDRIVEFANMIRDL
DQECGSTCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK
>Mature_614_residues
MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAYWPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALL
VVACDDGVMAQTREHLAILQLTGNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
LLQLPGREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRVRALHAQNQPTETAHAGQRIALN
IAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHTPLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDR
LVLRDISARNTLAGARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGEGMRELLQQ
PGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMALPMEDEALVLLLIEKMRESGDILSHHGWLHL
PDHKAGFSEEQQAIWQKAEPLFSDEPWWVRDLAKETGTDEQAMRLTLSQAAQQGIITAIVKDRYYRNDRIVEFANMIRDL
DQECGSTCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK

Specific function: Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP [H]

COG id: COG3276

COG function: function code J; Selenocysteine-specific translation elongation factor

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. SelB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=371, Percent_Identity=29.6495956873315, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI54607086, Length=285, Percent_Identity=26.3157894736842, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI5729864, Length=376, Percent_Identity=25.2659574468085, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI223555963, Length=376, Percent_Identity=25.2659574468085, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI53729339, Length=241, Percent_Identity=29.4605809128631, Blast_Score=75, Evalue=3e-13,
Organism=Homo sapiens, GI53729337, Length=241, Percent_Identity=29.4605809128631, Blast_Score=75, Evalue=3e-13,
Organism=Homo sapiens, GI82546879, Length=321, Percent_Identity=24.2990654205607, Blast_Score=75, Evalue=3e-13,
Organism=Homo sapiens, GI45593140, Length=323, Percent_Identity=24.1486068111455, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI194097354, Length=311, Percent_Identity=24.1157556270096, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI194018520, Length=311, Percent_Identity=24.1157556270096, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI194018522, Length=387, Percent_Identity=22.9974160206718, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI46094014, Length=383, Percent_Identity=22.976501305483, Blast_Score=67, Evalue=6e-11,
Organism=Escherichia coli, GI2367247, Length=614, Percent_Identity=97.7198697068404, Blast_Score=1225, Evalue=0.0,
Organism=Escherichia coli, GI1790412, Length=383, Percent_Identity=30.0261096605744, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789737, Length=377, Percent_Identity=30.2387267904509, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789559, Length=251, Percent_Identity=29.4820717131474, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789108, Length=293, Percent_Identity=25.938566552901, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25141371, Length=293, Percent_Identity=32.7645051194539, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17556456, Length=407, Percent_Identity=27.5184275184275, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17506081, Length=260, Percent_Identity=26.5384615384615, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI115532067, Length=304, Percent_Identity=27.3026315789474, Blast_Score=96, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI115532065, Length=304, Percent_Identity=27.3026315789474, Blast_Score=96, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17552884, Length=404, Percent_Identity=23.5148514851485, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17569207, Length=404, Percent_Identity=23.5148514851485, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI32566629, Length=300, Percent_Identity=26, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71994658, Length=234, Percent_Identity=29.9145299145299, Blast_Score=76, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=303, Percent_Identity=32.3432343234323, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6325337, Length=401, Percent_Identity=23.1920199501247, Blast_Score=80, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6319594, Length=401, Percent_Identity=23.1920199501247, Blast_Score=80, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6324550, Length=173, Percent_Identity=28.3236994219653, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320377, Length=305, Percent_Identity=25.2459016393443, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI281363316, Length=281, Percent_Identity=34.1637010676157, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI17864358, Length=281, Percent_Identity=34.1637010676157, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI19921738, Length=284, Percent_Identity=32.7464788732394, Blast_Score=129, Evalue=8e-30,
Organism=Drosophila melanogaster, GI19922690, Length=263, Percent_Identity=30.0380228136882, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI45550900, Length=377, Percent_Identity=23.0769230769231, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17137380, Length=350, Percent_Identity=24.8571428571429, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24652838, Length=407, Percent_Identity=21.6216216216216, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI17137572, Length=407, Percent_Identity=21.6216216216216, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI45553807, Length=371, Percent_Identity=23.1805929919137, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI45553816, Length=371, Percent_Identity=23.1805929919137, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24651721, Length=371, Percent_Identity=23.1805929919137, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17864154, Length=371, Percent_Identity=23.1805929919137, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28572034, Length=161, Percent_Identity=32.9192546583851, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015190
- InterPro:   IPR015191
- InterPro:   IPR000795
- InterPro:   IPR004161
- InterPro:   IPR009000
- InterPro:   IPR004535
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF09106 SelB-wing_2; PF09107 SelB-wing_3 [H]

EC number: NA

Molecular weight: Translated: 68806; Mature: 68806

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAYWPQPDGRVPSFIDVPG
CEEEECCCCCCCHHHHHHHHHCCCHHCCCHHHHCCCEEEECEEECCCCCCCCCCEEECCC
HEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLTGNPMLTVALTKADRVDEA
HHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHCCEEEEEECCCCEEEEEEECCCCCCHH
RVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREHLLQLPGREHASQHSFRLAID
HHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEE
RAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRVRALHAQNQPTETAHAGQRIALN
EEEEEECCCEEEEECEECCCEEECCCEEEEECCCCEEEEEEECCCCCCHHHHCCCEEEEE
IAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHTPLTQWQPLHIHHAASHVTGRVSLL
ECCCCCHHHCCCCCEEEECCCCCHHHHHHEEEECCCCCCCCCCEEEEHHHHHHHHHHHHH
EDNLAELVFDTPLWLADNDRLVLRDISARNTLAGARVVMLNPPRRGKRKPEYLQWLASLA
HHHHHHHHHCCCEEEECCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHH
RAQNDSEALAVHLQRGAVNLPDFAWARQLNGEGMRELLQQPGYIQAGYSLLNAPVAARWQ
HCCCCCCEEEEEEECCCCCCCCHHHHHHCCCHHHHHHHHCCCCCHHHHHHHCCCHHHHHH
RKILDTLATYHEQHRDEPGPGRERLRRMALPMEDEALVLLLIEKMRESGDILSHHGWLHL
HHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEECCCCEEEC
PDHKAGFSEEQQAIWQKAEPLFSDEPWWVRDLAKETGTDEQAMRLTLSQAAQQGIITAIV
CCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHH
KDRYYRNDRIVEFANMIRDLDQECGSTCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRG
HHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEECCC
NDHLLRDALLFPEK
CCHHHHHHHCCCCC
>Mature Secondary Structure
MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAYWPQPDGRVPSFIDVPG
CEEEECCCCCCCHHHHHHHHHCCCHHCCCHHHHCCCEEEECEEECCCCCCCCCCEEECCC
HEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLTGNPMLTVALTKADRVDEA
HHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHCCEEEEEECCCCEEEEEEECCCCCCHH
RVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREHLLQLPGREHASQHSFRLAID
HHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEE
RAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRVRALHAQNQPTETAHAGQRIALN
EEEEEECCCEEEEECEECCCEEECCCEEEEECCCCEEEEEEECCCCCCHHHHCCCEEEEE
IAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHTPLTQWQPLHIHHAASHVTGRVSLL
ECCCCCHHHCCCCCEEEECCCCCHHHHHHEEEECCCCCCCCCCEEEEHHHHHHHHHHHHH
EDNLAELVFDTPLWLADNDRLVLRDISARNTLAGARVVMLNPPRRGKRKPEYLQWLASLA
HHHHHHHHHCCCEEEECCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHH
RAQNDSEALAVHLQRGAVNLPDFAWARQLNGEGMRELLQQPGYIQAGYSLLNAPVAARWQ
HCCCCCCEEEEEEECCCCCCCCHHHHHHCCCHHHHHHHHCCCCCHHHHHHHCCCHHHHHH
RKILDTLATYHEQHRDEPGPGRERLRRMALPMEDEALVLLLIEKMRESGDILSHHGWLHL
HHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEECCCCEEEC
PDHKAGFSEEQQAIWQKAEPLFSDEPWWVRDLAKETGTDEQAMRLTLSQAAQQGIITAIV
CCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHH
KDRYYRNDRIVEFANMIRDLDQECGSTCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRG
HHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEECCC
NDHLLRDALLFPEK
CCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2531290; 8041620; 9278503; 2140572 [H]