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The map label for this gene is hybC

Identifier: 82545268

GI number: 82545268

Start: 2872317

End: 2874020

Strand: Direct

Name: hybC

Synonym: SBO_2869

Alternate gene names: 82545268

Gene position: 2872317-2874020 (Clockwise)

Preceding gene: 82545267

Following gene: 82545269

Centisome position: 63.55

GC content: 53.05

Gene sequence:

>1704_bases
ATGAGCCAGAGAATTACTATTGATCCGGTAACCCGTATTGAGGGGCATTTACGCATCGATTGCGAAATCGAAAATGGCGT
CGTTTCGAAAGCATGGGCTTCCGGTACCATGTGGCGCGGCATGGAAGAGATCGTGAAAAACCGCGATCCGCGCGATGCAT
GGATGATTGTGCAACGTATCTGTGGCGTATGTACTACCACTCACGCGCTGTCTTCCGTTCGTGCGGCAGAAAGTGCGCTG
AATATCGACGTTCCGGTTAACGCGCAATACATCCGTAACATCATTCTGGCTGCGCACACCACGCATGACCATATCGTTCA
TTTCTATCAGCTTTCGGCGCTGGACTGGGTGGACATCACTTCTGCACTGCAAGCTGACCCAACCAAAGCCTCCGAAATGC
TGAAAGGCGTTTCGACCTGGCATCTGAACAGTCCGGAAGAGTTCACTAAAGTTCAGAACAAGATCAAAGATCTGGTTGCC
AGCGGTCAGTTGGGTATTTTCGCCAATGGCTACTGGGGTCACCCGGCGATGAAACTGCCGCCGGAAGTGAACCTGATTGC
GGTAGCGCACTACCTGCAGGCGCTGGAGTGCCAGCGTGACGCTAACCGCGTCGTGGCGCTGCTGGGCGGTAAAACGCCGC
ACATTCAGAACCTGGCGGTAGGTGGTGTCGCGAACCCGATCAACCTCGACGGTTTGGGCGTGCTGAACCTTGAGCGCCTG
ATGTACATCAAGTCTTTCATCGACAAGCTGAGCGACTTTGTTGAACAGGTTTATAAGGTTGATACCGCAGTTATTGCCGC
GTTCTACCCGGAATGGCTGACGCGCGGTAAAGGTGCGGTGAACTACCTGAGCGTGCCGGAATTCCCGACCGACAGTAAAA
ACGGCAGCTTCCTGTTCCCGGGCGGCTACATTGAGAATGCGGATCTGTCCTCGTATCGTCCGATCACTTCTCATTCCGAT
GAATACCTGATCAAAGGCATTCAGGAAAGCGCGAAGCACTCCTGGTATAAAGACGAAGCGCCGCAGGCACCGTGGGAAGG
TACCACCATTCCGGCTTATGATGGTTGGTCTGACGACGGGAAATATTCCTGGGTGAAATCACCGACTTTCTACGGCAAAA
CGGTAGAAGTGGGTCCGCTGGCTAATATGCTGGTGAAACTGGCGGCAGGTCGCGAATCTACCCAGAACAAACTGAATGAA
ATCGTTGCGATTTATCAGAAACTGACTGGCAACACGCTGGAAGTGGCGCAGCTGCACTCCACGCTGGGCCGTATTATTGG
TCGTACCGTTCACTGCTGCGAATTGCAGGATATCCTGCAAAATCAATACAGTGCACTGATCACCAATATCGGCAAAGGCG
ATCACACCACCTTTGTGAAACCGAACATTCCGGCAACGGGTGAGTTCAAAGGTGTTGGCTTCCTCGAAGCGCCGCGCGGT
ATGCTCTCTCACTGGATGGTTATTAAAGACGGTATTATCAGTAACTACCAGGCAGTTGTTCCATCAACCTGGAACTCTGG
TCCGCGTAACTTCAATGATGACGTCGGTCCTTACGAGCAGTCGCTGGTGGGTACACCGGTTGCTGATCCGAATAAACCGC
TGGAAGTGGTGCGTACCATTCACTCCTTCGACCCGTGCATGGCCTGTGCGGTACACGTAGTGGATGCCGACGGCAACGAA
GTGGTTTCAGTGAAGGTTCTGTAA

Upstream 100 bases:

>100_bases
TGGCTATTGAGATTTGCGCTTACATCGTACTCATTCGTCTACTGCCGATACTTCCTCCTTTAAAACAAAACGATCATAAT
CGTCATGAGGCGAGCAAAGC

Downstream 100 bases:

>100_bases
TGCGTATTTTAGTCTTAGGGGTCGGCAATATTTTGCTGACCGATGAAGCCATCGGTGTGCGGATTGTCGAAGCGTTAGAG
CAACGATACATTCTGCCGGA

Product: hydrogenase 2 large subunit

Products: NA

Alternate protein names: HYD2; Membrane-bound hydrogenase 2 large subunit; NiFe hydrogenase

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEIVKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESAL
NIDVPVNAQYIRNIILAAHTTHDHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNLAVGGVANPINLDGLGVLNLERL
MYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRGKGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSD
EYLIKGIQESAKHSWYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRESTQNKLNE
IVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNIGKGDHTTFVKPNIPATGEFKGVGFLEAPRG
MLSHWMVIKDGIISNYQAVVPSTWNSGPRNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNE
VVSVKVL

Sequences:

>Translated_567_residues
MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEIVKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESAL
NIDVPVNAQYIRNIILAAHTTHDHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNLAVGGVANPINLDGLGVLNLERL
MYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRGKGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSD
EYLIKGIQESAKHSWYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRESTQNKLNE
IVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNIGKGDHTTFVKPNIPATGEFKGVGFLEAPRG
MLSHWMVIKDGIISNYQAVVPSTWNSGPRNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNE
VVSVKVL
>Mature_566_residues
SQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEIVKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALN
IDVPVNAQYIRNIILAAHTTHDHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVAS
GQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNLAVGGVANPINLDGLGVLNLERLM
YIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRGKGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDE
YLIKGIQESAKHSWYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRESTQNKLNEI
VAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNIGKGDHTTFVKPNIPATGEFKGVGFLEAPRGM
LSHWMVIKDGIISNYQAVVPSTWNSGPRNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEV
VSVKVL

Specific function: This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family

Homologues:

Organism=Escherichia coli, GI1789368, Length=567, Percent_Identity=100, Blast_Score=1178, Evalue=0.0,
Organism=Escherichia coli, GI1787207, Length=591, Percent_Identity=42.8087986463621, Blast_Score=481, Evalue=1e-137,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MBHM_ECO57 (P0ACE1)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   G85958
- PIR:   G91113
- RefSeq:   NP_289572.1
- RefSeq:   NP_311906.1
- ProteinModelPortal:   P0ACE1
- SMR:   P0ACE1
- MINT:   MINT-1254855
- EnsemblBacteria:   EBESCT00000026512
- EnsemblBacteria:   EBESCT00000056879
- GeneID:   916295
- GeneID:   958476
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4348
- KEGG:   ecs:ECs3879
- GeneTree:   EBGT00050000010530
- HOGENOM:   HBG733231
- OMA:   VHFYHLS
- ProtClustDB:   PRK10467
- BioCyc:   ECOL83334:ECS3879-MONOMER
- InterPro:   IPR001501
- InterPro:   IPR018194

Pfam domain/function: PF00374 NiFeSe_Hases

EC number: =1.12.99.6

Molecular weight: Translated: 62492; Mature: 62361

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00507 NI_HGENASE_L_1; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEIVKNRDPRDAWMIVQRI
CCCCEECCCHHHCCCEEEEEEEECCCEEHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHH
CGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTHDHIVHFYQLSALDWVDIT
HHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHEECCHHHHEEEHHHCCCCHHHHH
SALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVASGQLGIFANGYWGHPAMKLP
HHHCCCCHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
PEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNLAVGGVANPINLDGLGVLNLERL
CCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEECCCCCCCCCCCCCHHHHHHH
MYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRGKGAVNYLSVPEFPTDSKNGSFLFP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEECC
GGYIENADLSSYRPITSHSDEYLIKGIQESAKHSWYKDEAPQAPWEGTTIPAYDGWSDDG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KYSWVKSPTFYGKTVEVGPLANMLVKLAAGRESTQNKLNEIVAIYQKLTGNTLEVAQLHS
CEEEECCCCCCCCEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TLGRIIGRTVHCCELQDILQNQYSALITNIGKGDHTTFVKPNIPATGEFKGVGFLEAPRG
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCHHCCHH
MLSHWMVIKDGIISNYQAVVPSTWNSGPRNFNDDVGPYEQSLVGTPVADPNKPLEVVRTI
HHHHHHHHHCCHHCCCEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHH
HSFDPCMACAVHVVDADGNEVVSVKVL
HHCCHHHHHEEEEEECCCCEEEEEEEC
>Mature Secondary Structure 
SQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEIVKNRDPRDAWMIVQRI
CCCEECCCHHHCCCEEEEEEEECCCEEHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHH
CGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTHDHIVHFYQLSALDWVDIT
HHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHEECCHHHHEEEHHHCCCCHHHHH
SALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVASGQLGIFANGYWGHPAMKLP
HHHCCCCHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
PEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNLAVGGVANPINLDGLGVLNLERL
CCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEECCCCCCCCCCCCCHHHHHHH
MYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRGKGAVNYLSVPEFPTDSKNGSFLFP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEECC
GGYIENADLSSYRPITSHSDEYLIKGIQESAKHSWYKDEAPQAPWEGTTIPAYDGWSDDG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KYSWVKSPTFYGKTVEVGPLANMLVKLAAGRESTQNKLNEIVAIYQKLTGNTLEVAQLHS
CEEEECCCCCCCCEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TLGRIIGRTVHCCELQDILQNQYSALITNIGKGDHTTFVKPNIPATGEFKGVGFLEAPRG
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCHHCCHH
MLSHWMVIKDGIISNYQAVVPSTWNSGPRNFNDDVGPYEQSLVGTPVADPNKPLEVVRTI
HHHHHHHHHCCHHCCCEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHH
HSFDPCMACAVHVVDADGNEVVSVKVL
HHCCHHHHHEEEEEECCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796