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The map label for this gene is hybA

Identifier: 82545266

GI number: 82545266

Start: 2870166

End: 2871152

Strand: Direct

Name: hybA

Synonym: SBO_2867

Alternate gene names: 82545266

Gene position: 2870166-2871152 (Clockwise)

Preceding gene: 82545265

Following gene: 82545267

Centisome position: 63.5

GC content: 54.71

Gene sequence:

>987_bases
GTGAACAGACGTAATTTTATTAAAGCAGCCTCCTGCGGGGCATTGCTGACGGGCGCGCTGCCGTCTGTCAGTCATGCGGC
TGCTGAAAACCGCCCGCCAATTCCGGGATCGCTGGGGATGTTGTACGACTCGACCTTGTGCGTAGGCTGCCAGGCTTGCG
TCACCAAGTGTCAGGATATCAACTTCCCTGAACGTAACCCGCAAGGGGAACAGACCTGGTCGAACAACGACAAACTGTCG
CCGTATACCAATAACATCATTCAGGTGTGGACCAGCGGCACAGGGGTCAACAAAGACCAGGAGGAGAACGGCTACGCGTA
CATTAAGAAACAGTGTATGCACTGCGTCGATCCGAACTGTGTCTCTGTGTGCCCGGTCTCTGCACTGAAAAAAGATCCGA
AAACCGGCATTGTCCATTACGACAAAGACGTGTGCACCGGTTGCCGTTACTGCATGGTCGCCTGTCCGTACAACGTGCCG
AAGTACGACTACAACAACCCGTTTGGTGCGCTGCATAAGTGCGAGCTGTGCAACCAGAAAGGTGTGGAACGTCTCGATAA
AGGCGGTCTGCCTGGCTGCGTAGAAGTGTGCCCGGCGGGCGCGGTGATTTTCGGTACGCGTGAAGAGCTGATGGCGGAGG
CGAAAAAACGTCTGGCGCTGAAGCCTGGCAGCGAATACCACTATCCGCGTCAGACGCTGAAATCTGGCGACACTTACCTG
CATACGGTGCCGAAATATTATCCGCATCTGTACGGCGAGAAAGAGGGCGGCGGTACTCAGGTTCTGGTACTGACGGGTGT
GCCTTATGAAAATCTCGACCTGCCGAAACTGGACGATCTTTCTACCGGTGCGCGTTCCGAAAATATTCAACACACCCTGT
ATAAAGGCATGATGCTACCACTGGCTGTGCTGGCGGGCTTAACCGTGCTGGTTCGTCGCAACACCAAAAACGACCATCAC
GACGGAGGAGACGATCATGAGTCATGA

Upstream 100 bases:

>100_bases
GCGGTGTGGTTGGGCTGGTTGCCGGTGTCAGCGTGATGGCGGTGCGTGAACTGGGTCGTCAGCAAAAGAAAGATAACGCT
GACTCACGGGGAGAATAACC

Downstream 100 bases:

>100_bases
TCCACAACCGCTGGGCGGCAAAATCATCAGTAAACCGGTCATGATTTTTGGACCGTTAATCGTCATCTGTATGCTCCTGA
TTGTGAAGCGTCTGGTGTTC

Product: hydrogenase 2 protein HybA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDSTLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLS
PYTNNIIQVWTSGTGVNKDQEENGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRLALKPGSEYHYPRQTLKSGDTYL
HTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPKLDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHH
DGGDDHES

Sequences:

>Translated_328_residues
MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDSTLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLS
PYTNNIIQVWTSGTGVNKDQEENGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRLALKPGSEYHYPRQTLKSGDTYL
HTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPKLDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHH
DGGDDHES
>Mature_328_residues
MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDSTLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLS
PYTNNIIQVWTSGTGVNKDQEENGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRLALKPGSEYHYPRQTLKSGDTYL
HTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPKLDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHH
DGGDDHES

Specific function: Participates in the periplasmic electron-transferring activity of hydrogenase 2 during its catalytic turnover

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Escherichia coli, GI1789370, Length=328, Percent_Identity=100, Blast_Score=681, Evalue=0.0,
Organism=Escherichia coli, GI2367345, Length=255, Percent_Identity=30.5882352941176, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787749, Length=283, Percent_Identity=27.5618374558304, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790326, Length=184, Percent_Identity=33.1521739130435, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1787872, Length=198, Percent_Identity=34.3434343434343, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1787122, Length=198, Percent_Identity=33.8383838383838, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI226510944, Length=215, Percent_Identity=30.6976744186047, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1787478, Length=133, Percent_Identity=33.8345864661654, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787740, Length=99, Percent_Identity=37.3737373737374, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082180, Length=192, Percent_Identity=29.1666666666667, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HYBA_ECO57 (P0AAJ9)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A91114
- RefSeq:   NP_289574.1
- RefSeq:   NP_311908.1
- ProteinModelPortal:   P0AAJ9
- SMR:   P0AAJ9
- EnsemblBacteria:   EBESCT00000027749
- EnsemblBacteria:   EBESCT00000057009
- GeneID:   916288
- GeneID:   958469
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4350
- KEGG:   ecs:ECs3881
- GeneTree:   EBGT00070000031694
- HOGENOM:   HBG726839
- OMA:   CPTGAVI
- ProtClustDB:   PRK10882
- BioCyc:   ECOL83334:ECS3881-MONOMER
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311
- TIGRFAMs:   TIGR01409

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 36003; Mature: 36003

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
5.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDSTLCVGCQACVTKCQDI
CCCCHHHHHHHCCHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
NFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEENGYAYIKKQCMHCVDPNC
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCC
VSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVPKYDYNNPFGALHKCELCNQK
HHCCCHHHHHCCCCCCCEEECHHHHHHHHHEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
GVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRLALKPGSEYHYPRQTLKSGDTYL
HHHHHHCCCCCHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEE
HTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPKLDDLSTGARSENIQHTLYKGMMLP
EHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LAVLAGLTVLVRRNTKNDHHDGGDDHES
HHHHHHHHEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDSTLCVGCQACVTKCQDI
CCCCHHHHHHHCCHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
NFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEENGYAYIKKQCMHCVDPNC
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCC
VSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVPKYDYNNPFGALHKCELCNQK
HHCCCHHHHHCCCCCCCEEECHHHHHHHHHEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
GVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRLALKPGSEYHYPRQTLKSGDTYL
HHHHHHCCCCCHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEE
HTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPKLDDLSTGARSENIQHTLYKGMMLP
EHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LAVLAGLTVLVRRNTKNDHHDGGDDHES
HHHHHHHHEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796