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The map label for this gene is eutB [H]

Identifier: 82544902

GI number: 82544902

Start: 2456408

End: 2458030

Strand: Reverse

Name: eutB [H]

Synonym: SBO_2468

Alternate gene names: 82544902

Gene position: 2458030-2456408 (Counterclockwise)

Preceding gene: 82544903

Following gene: 82544900

Centisome position: 54.38

GC content: 56.93

Gene sequence:

>1623_bases
ATGCAAAATGAATTGCAGACCGCGCTCTTTCAGGCGTTCGATACCCTGAATCTGCAACGGGTAAAAACATTTAGCGTTCC
ACCGGTGACGCTTTGCGGTCCGGGCTCGGTGAGCAGTTGCGGACAGCAAGCGCAAACGCGTGGGCTGAAACATCTGTTCG
TGATGGCAGACAGCTTTTTGCATCAGGCAGGGATGACCGCCGGGCTGACGCGCAGCCTGGCTGTTAAAGGCATCGCCATG
ACGCTCTGGCCATGTCCGGTGGGCGAACCGTGCATCACCGACGTGTGTGCAGCCGTGGCGCAGTTGCGTGAGTCAGGCTG
TGATGGGGTGATCGCATTTGGCGGCGGCTCGGTGCTGGATGCGGCGAAAGCCGTGGCGTTGCTGGTGACGAACCCGGATA
GCACGCTGGCAGAGATGTCAGAAACCAGCGTTCTGCAACCGCGCTTGCCGCTGATTGCCTATGAAGATGACTGCGTGACG
CGGCTGATTCAGGACGATGTTAACGAAACGGCCTACAACCAGATTAAAAACTGGAGCATCAGCGAACTGCGTGAGTATGT
GCTGAGCGATGAAACTAGCGTGGACGACATTGCCTTTACCCGCAAAGGGCTGACCTCGGAAGTGGTCGCGGCGGTAGCGA
AGATTTGCTCCAACGCGGACCTGATCTACGGCGCGAAGAAAATGCCGGTAATCAAAAAGGCCAATACCACCATCGGTATT
CCGGGCACCTTCAGCGCCCGTTTGCAGCCAAATGACACTCGTGACGACGTGCAAAGTATCGCCGCGCAAATCTACGAAGG
GCTTTCCTTCGGGGTGGGCGATGCGGTGATCGGCGTCAACCCGGTAACTGACGACGTGGAAAACTTAAGCCGCGTGCTGG
ATACCATTTATGGCGTGATCGACAAATTCAACATCCCAACTCAGGGCTGCGTACTGGCGCACGTCACCACCCAGATCGAA
GCGATTCGTCGCGGCGCGCCTGGCGGACTGATTTTCCAGAGTATCTGTGGCAGCGAAAAAGGGCTGAAAGAGTTTGGCGT
GGAACTGGCGATGCTCGACGAAGCGCGCGCAGTGGGTGCAGAGTTCAACCGTATCGCCGGGGAAAACTGCCTCTACTTCG
AAACCGGACAAGGCTCTGCGCTATCCGCTGGCGCTAACTTCGGCGCTGACCAGGTGACGATGGAAGCACGTAACTATGGG
CTGGCGCGTCATTACGATCCGTTTATCGTCAACACCGTGGTCGGCTTTATTGGGCCGGAGTATCTCTACAACGACCGCCA
GATTATCCGTGCTGGCTTAGAAGATCACTTTATGGGCAAGCTGAGCGGCATCTCTATGGGCTGTGACTGCTGCTACACCA
ACCACGCTGACGCTGACCAGAACCTCAACGAAAACCTGATGATCCTGCTCGCCACCGCAGGCTGCAACTACATCATGGGG
ATGCCGCTGGGTGATGACATCATGCTCAACTACCAGACCACCGCATTCCACGATACCGCCACTGTGCGTCAGTTACTCAA
CCTGCGCCCGTCACCAGAGTTTGAACGCTGGCTGGAAAGCATGGGCATTATGGCAAACGGTCGCCTGACCAAACGGGCGG
GCGATCCGTCACTGTTCTTCTGA

Upstream 100 bases:

>100_bases
AAACAATTCCCGGCGTTACAGGTGCATTTACCGCAGCACAGTTTGTTTATGACCCCGCTGGCGATCGCCAGTAGTGGGCG
AGAGAAAGCGGAGGGGCTAT

Downstream 100 bases:

>100_bases
TGACGCGGAGATAACACATCATGGATCAAAAACAGATTGAAGAAATTGTACGCAGCGTGATGGCGTCAATGGGACAAACG
GGTAATGCTGCCAACTTACT

Product: ethanolamine ammonia-lyase, heavy chain

Products: NA

Alternate protein names: Ethanolamine ammonia-lyase large subunit [H]

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQAQTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAM
TLWPCPVGEPCITDVCAAVAQLRESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAYEDDCVT
RLIQDDVNETAYNQIKNWSISELREYVLSDETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGI
PGTFSARLQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNIPTQGCVLAHVTTQIE
AIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFNRIAGENCLYFETGQGSALSAGANFGADQVTMEARNYG
LARHYDPFIVNTVVGFIGPEYLYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIMG
MPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPSLFF

Sequences:

>Translated_540_residues
MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQAQTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAM
TLWPCPVGEPCITDVCAAVAQLRESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAYEDDCVT
RLIQDDVNETAYNQIKNWSISELREYVLSDETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGI
PGTFSARLQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNIPTQGCVLAHVTTQIE
AIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFNRIAGENCLYFETGQGSALSAGANFGADQVTMEARNYG
LARHYDPFIVNTVVGFIGPEYLYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIMG
MPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPSLFF
>Mature_540_residues
MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQAQTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAM
TLWPCPVGEPCITDVCAAVAQLRESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAYEDDCVT
RLIQDDVNETAYNQIKNWSISELREYVLSDETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGI
PGTFSARLQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNIPTQGCVLAHVTTQIE
AIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFNRIAGENCLYFETGQGSALSAGANFGADQVTMEARNYG
LARHYDPFIVNTVVGFIGPEYLYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIMG
MPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPSLFF

Specific function: Ethanolamine utilization. [C]

COG id: COG4303

COG function: function code E; Ethanolamine ammonia-lyase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082106, Length=403, Percent_Identity=97.7667493796526, Blast_Score=817, Evalue=0.0,
Organism=Escherichia coli, GI87082107, Length=212, Percent_Identity=77.3584905660377, Blast_Score=315, Evalue=4e-87,
Organism=Escherichia coli, GI48994951, Length=131, Percent_Identity=28.2442748091603, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1789163, Length=174, Percent_Identity=27.0114942528736, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010628 [H]

Pfam domain/function: PF06751 EutB [H]

EC number: =4.3.1.7 [H]

Molecular weight: Translated: 58244; Mature: 58244

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQAQTRGLKHLFVMADSFL
CCHHHHHHHHHHHHCCCHHHEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
HQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQLRESGCDGVIAFGGGSVLD
HHHCCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHH
AAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAYEDDCVTRLIQDDVNETAYNQIKNWSI
HHCEEEEEEECCCHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCH
SELREYVLSDETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGI
HHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCCCCCEEEECCCEEEC
PGTFSARLQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVI
CCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHH
DKFNIPTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGA
HCCCCCCCCEEEHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHCCEEEEHHHHHHHHH
EFNRIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPE
HHHHHCCCCEEEEECCCCCEEECCCCCCCHHEEHHHHHCCCHHCCCCHHHHHHHHHHCHH
YLYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIMG
HHHCCHHHHHHCHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCEEEEEEECCCCEEEE
MPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPSLFF
CCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHCCCEECCEEEECCCCCCCCC
>Mature Secondary Structure
MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQAQTRGLKHLFVMADSFL
CCHHHHHHHHHHHHCCCHHHEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
HQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQLRESGCDGVIAFGGGSVLD
HHHCCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHH
AAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAYEDDCVTRLIQDDVNETAYNQIKNWSI
HHCEEEEEEECCCHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCH
SELREYVLSDETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGI
HHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCCCCCEEEECCCEEEC
PGTFSARLQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVI
CCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHH
DKFNIPTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGA
HCCCCCCCCEEEHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHCCEEEEHHHHHHHHH
EFNRIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPE
HHHHHCCCCEEEEECCCCCEEECCCCCCCHHEEHHHHHCCCHHCCCCHHHHHHHHHHCHH
YLYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIMG
HHHCCHHHHHHCHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCEEEEEEECCCCEEEE
MPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPSLFF
CCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHCCCEECCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]