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The map label for this gene is fryB

Identifier: 82544855

GI number: 82544855

Start: 2409846

End: 2410172

Strand: Reverse

Name: fryB

Synonym: SBO_2413

Alternate gene names: 82544855

Gene position: 2410172-2409846 (Counterclockwise)

Preceding gene: 82544856

Following gene: 82544854

Centisome position: 53.32

GC content: 50.46

Gene sequence:

>327_bases
ATGAGTAAGAAACTGATTGCCTTATGTGCCTGCCCAATGGGCCTGGCTCACACCTTTATGGCCGCTCAGGCGTTGGAAGA
AGCGGCGGTAGAAGCCGGTTATGAAGTGAAAATTGAAACCCAGGGCGCGGACGGTATCCAGAATCGCCTGACGGCGCAGG
ATATCGCCGAAGCAACCATCATCATCCACTCCGTGGCAGTTACCCCGGAAGATAATGAGCGTTTCGAATCACGCGACGTT
TATGAAATCACTTTGCAGGACGCAATTAAAAACGCTGCGGGCATCATCAAAGAAATCGAAGAGATGATTGCCTCTGAACA
ACAGTAA

Upstream 100 bases:

>100_bases
GATTAATTCTCCAGTAAATGCATTATTTGTCTGGTAACGGCGATTTGTTTTGCACGTTCATAATTTCACTTGTCAACTAT
CACTGAATGAGGAATTAACG

Downstream 100 bases:

>100_bases
TATTCGACAGGGATAATTGTTATGGCCATTAAAAAACGCAGTGCAACCGTTGTGCCTGGCGTATCCGGTGCGGCAGCGGC
AGTTAAGAATCCGCAGGCCT

Product: PTS system enzyme IIB component

Products: NA

Alternate protein names: PTS system fructose-like EIIB component 1

Number of amino acids: Translated: 108; Mature: 107

Protein sequence:

>108_residues
MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGADGIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDV
YEITLQDAIKNAAGIIKEIEEMIASEQQ

Sequences:

>Translated_108_residues
MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGADGIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDV
YEITLQDAIKNAAGIIKEIEEMIASEQQ
>Mature_107_residues
SKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGADGIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVY
EITLQDAIKNAAGIIKEIEEMIASEQQ

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane

COG id: COG1445

COG function: function code G; Phosphotransferase system fructose-specific component IIB

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-2 domain

Homologues:

Organism=Escherichia coli, GI1788730, Length=108, Percent_Identity=100, Blast_Score=217, Evalue=1e-58,
Organism=Escherichia coli, GI1790387, Length=101, Percent_Identity=45.5445544554455, Blast_Score=97, Evalue=2e-22,
Organism=Escherichia coli, GI87082348, Length=100, Percent_Identity=40, Blast_Score=89, Evalue=6e-20,
Organism=Escherichia coli, GI1786951, Length=103, Percent_Identity=34.9514563106796, Blast_Score=74, Evalue=1e-15,
Organism=Escherichia coli, GI1788492, Length=103, Percent_Identity=37.8640776699029, Blast_Score=69, Evalue=5e-14,
Organism=Escherichia coli, GI1790390, Length=104, Percent_Identity=31.7307692307692, Blast_Score=62, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTFB1_ECO57 (P69810)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C91037
- PIR:   E85881
- RefSeq:   NP_288957.1
- RefSeq:   NP_311294.1
- ProteinModelPortal:   P69810
- SMR:   P69810
- EnsemblBacteria:   EBESCT00000025413
- EnsemblBacteria:   EBESCT00000057017
- GeneID:   915630
- GeneID:   959383
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3653
- KEGG:   ecs:ECs3267
- GeneTree:   EBGT00070000031719
- HOGENOM:   HBG564028
- OMA:   NEELTMS
- ProtClustDB:   CLSK880379
- BioCyc:   ECOL83334:ECS3267-MONOMER
- GO:   GO:0005737
- GO:   GO:0016020
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003353
- TIGRFAMs:   TIGR00829

Pfam domain/function: PF02302 PTS_IIB

EC number: =2.7.1.69

Molecular weight: Translated: 11735; Mature: 11604

Theoretical pI: Translated: 4.11; Mature: 4.11

Prosite motif: PS51099 PTS_EIIB_TYPE_2

Important sites: ACT_SITE 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGADGIQNRLTAQDIAEATI
CCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHH
IIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEMIASEQQ
HHHEEEECCCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGADGIQNRLTAQDIAEATI
CCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHH
IIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEMIASEQQ
HHHEEEECCCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796