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The map label for this gene is emrY

Identifier: 82544835

GI number: 82544835

Start: 2382570

End: 2384108

Strand: Reverse

Name: emrY

Synonym: SBO_2393

Alternate gene names: 82544835

Gene position: 2384108-2382570 (Counterclockwise)

Preceding gene: 82544836

Following gene: 82544833

Centisome position: 52.75

GC content: 41.65

Gene sequence:

>1539_bases
ATGGCAATCACTAAATCAACTCCGGCACCATTAACCGGTGGGACGTTATGGTGCGTCACTATTGCATTGTCATTAGCGAC
ATTTATGCAAATGTTGGATTCCACTATTTCTAACGTCGCAATACCGACAATATCTGGCTTTCTGGGAGCATCAACAGACG
AAGGCACCTGGGTTATCACCTCGTTTGGTGTAGCAAATGCCATTGCGATCCCTGTTACTGGCAGGTTGGCACAAAGAATA
GGCGAATTAAGATTATTTTTACTTTCAGTAACCTTTTTTTCGCTGTCTTCATTAATGTGTAGCCTATCGACCAATCTTGA
TGTGCTGATATTTTTTAGAGTCGTTCAGGGGTTAATGGCGGGGCCGTTAATTCCACTGTCACAGAGTTTATTATTAAGGA
ATTATCCGCCAGAAAAAAGAACATTTGCTCTGGCATTATGGTCAATGACCGTGATTATCGCTCCGATATGTGGGCCGATA
TTGGGCGGTTATATTTGTGATAACTTTAGCTGGGGTTGGATATTTTTAATCAATGTCCCTATGGGGATTATCGTCCTGAC
ATTATGCTTAACCTTACTTAAAGGAAGAGAAACTGAGACTTCACCGGTCAAAATGAATCTACCAGGACTGACCCTGTTAG
TGCTCGGTGTTGGTGGCTTGCAAATTATGCTTGATAAAGGGCGCGATCTGGATTGGTTCAACTCGAGTACAATAATAATA
TTAACAGTAGTATCAGTTATTTCTCTGATCTCTTTAGTCATTTGGGAGTCGACCTCAGAGAACCCGATTCTTGATCTCAG
TTTGTTTAAGTCCCGTAACTTCACCATTGGTATTGTGAGTATCACATGCGCGTATTTATTTTACTCTGGAGCGATCGTCC
TTATGCCGCAGTTACTCCAGGAAACGATGGGGTATAATGCGATATGGGCCGGACTTGCTTATGCGCCCATCGGCATCATG
CCACTATTAATTTCACCTTTGATAGGACGTTATGGCAACAAAATAGACATGCGGTTGTTAGTGACATTTAGTTTTTTGAT
GTATGCGGTTTGCTATTACTGGCGTTCTGTGACATTTATGCCAACGATTGATTTTACAGGCATCATTTTGCCGCAGTTTT
TTCAGGGATTCGCCGTTGCCTGTTTCTTTTTACCCTTAACAACGATTTCGTTTTCAGGCTTGCCAGATAATAAATTTGCC
AATGCCTCGAGTATGAGTAATTTTTTTCGTACCTTGTCAGGATCAGTTGGTACGTCGTTGACAATGACGCTGTGGGGACG
ACGCGAATCGTTACACCATAGTCAGTTGACAGCAACCATCGATCAATTTAACCCCGTGTTTAATTCATCGTCACAAATTA
TGGATAAATATTATGGTTCGCTTTCAGGAGTTCTTAATGAAATTAATAATGAAATAACCCAGCAGTCACTTTCTATTTCT
GCAAATGAGATTTTCCGTATGGCGGCTATTGCTTTTATCTTACTTACGGTTTTGGTTTGGTTTGCGAAACCGCCGTTTAC
AGCGAAAGGCGTTGGGTGA

Upstream 100 bases:

>100_bases
TGACCTCCATGCCGGCTTATACCAGTAAGGCTTTAGTTATCGATACCAGTCCGATAGAAAAAGAAATTAGCAACATTATT
TCGCATAATGGACAACTTTA

Downstream 100 bases:

>100_bases
TGATAAAAGGAGGGGGTTATAGCGGGTAAGACCGGGGATCTTAAGTCTGCGCCATCGGCAGCATCGCTCACCCAGGGAAA
GGATTGCGATGCTGCGTTGA

Product: multidrug resistance protein Y

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTISGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRI
GELRLFLLSVTFFSLSSLMCSLSTNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVGGLQIMLDKGRDLDWFNSSTIII
LTVVSVISLISLVIWESTSENPILDLSLFKSRNFTIGIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIM
PLLISPLIGRYGNKIDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFSGLPDNKFA
NASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSSSQIMDKYYGSLSGVLNEINNEITQQSLSIS
ANEIFRMAAIAFILLTVLVWFAKPPFTAKGVG

Sequences:

>Translated_512_residues
MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTISGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRI
GELRLFLLSVTFFSLSSLMCSLSTNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVGGLQIMLDKGRDLDWFNSSTIII
LTVVSVISLISLVIWESTSENPILDLSLFKSRNFTIGIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIM
PLLISPLIGRYGNKIDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFSGLPDNKFA
NASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSSSQIMDKYYGSLSGVLNEINNEITQQSLSIS
ANEIFRMAAIAFILLTVLVWFAKPPFTAKGVG
>Mature_511_residues
AITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTISGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIG
ELRLFLLSVTFFSLSSLMCSLSTNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPIL
GGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVGGLQIMLDKGRDLDWFNSSTIIIL
TVVSVISLISLVIWESTSENPILDLSLFKSRNFTIGIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMP
LLISPLIGRYGNKIDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFSGLPDNKFAN
ASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSSSQIMDKYYGSLSGVLNEINNEITQQSLSISA
NEIFRMAAIAFILLTVLVWFAKPPFTAKGVG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family

Homologues:

Organism=Escherichia coli, GI1788710, Length=512, Percent_Identity=100, Blast_Score=1023, Evalue=0.0,
Organism=Escherichia coli, GI1789042, Length=497, Percent_Identity=62.374245472837, Blast_Score=625, Evalue=1e-180,
Organism=Escherichia coli, GI1790195, Length=437, Percent_Identity=32.2654462242563, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1788392, Length=426, Percent_Identity=29.3427230046948, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI87081983, Length=418, Percent_Identity=26.7942583732057, Blast_Score=129, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6322958, Length=192, Percent_Identity=28.6458333333333, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325455, Length=153, Percent_Identity=28.7581699346405, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=28, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EMRY_ECOLI (P52600)

Other databases:

- EMBL:   D78168
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   D65010
- RefSeq:   AP_002967.1
- RefSeq:   NP_416868.1
- ProteinModelPortal:   P52600
- DIP:   DIP-9507N
- MINT:   MINT-1286081
- STRING:   P52600
- PRIDE:   P52600
- EnsemblBacteria:   EBESCT00000002698
- EnsemblBacteria:   EBESCT00000016002
- GeneID:   946835
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2364
- KEGG:   eco:b2367
- EchoBASE:   EB3068
- EcoGene:   EG13283
- eggNOG:   COG0477
- GeneTree:   EBGT00050000008825
- HOGENOM:   HBG759108
- OMA:   DFTGIIM
- ProtClustDB:   CLSK880371
- BioCyc:   EcoCyc:EMRY-MONOMER
- Genevestigator:   P52600
- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- TIGRFAMs:   TIGR00711

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 56002; Mature: 55871

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x19fe9074)-; HASH(0x1a80708c)-; HASH(0x19e8ef9c)-; HASH(0x1ad4d87c)-; HASH(0x1a3b761c)-; HASH(0x1addc950)-; HASH(0x19a29e58)-; HASH(0x1ad4d9a8)-; HASH(0x1abe8560)-; HASH(0x18c29f94)-; HASH(0x1970e3ac)-; HASH(0x1ad5ad60)-; HASH(0x1a9c79ac)-; HASH(0x1a63c990)-;

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTISGFLGASTDEGTWVIT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEE
SFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSLSTNLDVLIFFRVVQGLMA
ECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
GPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPILGGYICDNFSWGWIFLINVP
CCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
MGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVGGLQIMLDKGRDLDWFNSSTIII
HHHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEEECCCCEEEEEECCCCCCCCCCCCHHH
LTVVSVISLISLVIWESTSENPILDLSLFKSRNFTIGIVSITCAYLFYSGAIVLMPQLLQ
HHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEHHHHHHHHHHHCCHHHHHHHHHH
ETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNKIDMRLLVTFSFLMYAVCYYWRSVTFM
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEE
PTIDFTGIILPQFFQGFAVACFFLPLTTISFSGLPDNKFANASSMSNFFRTLSGSVGTSL
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEE
TMTLWGRRESLHHSQLTATIDQFNPVFNSSSQIMDKYYGSLSGVLNEINNEITQQSLSIS
EEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ANEIFRMAAIAFILLTVLVWFAKPPFTAKGVG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
>Mature Secondary Structure 
AITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTISGFLGASTDEGTWVIT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEE
SFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSLSTNLDVLIFFRVVQGLMA
ECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
GPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPILGGYICDNFSWGWIFLINVP
CCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
MGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVGGLQIMLDKGRDLDWFNSSTIII
HHHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEEECCCCEEEEEECCCCCCCCCCCCHHH
LTVVSVISLISLVIWESTSENPILDLSLFKSRNFTIGIVSITCAYLFYSGAIVLMPQLLQ
HHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEHHHHHHHHHHHCCHHHHHHHHHH
ETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNKIDMRLLVTFSFLMYAVCYYWRSVTFM
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEE
PTIDFTGIILPQFFQGFAVACFFLPLTTISFSGLPDNKFANASSMSNFFRTLSGSVGTSL
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEE
TMTLWGRRESLHHSQLTATIDQFNPVFNSSSQIMDKYYGSLSGVLNEINNEITQQSLSIS
EEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ANEIFRMAAIAFILLTVLVWFAKPPFTAKGVG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503